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Virus entry into a polarized epithelial cell line (MDCK)
Virus entry into a polarized epithelial cell line (MDCK)

... BCV or influenza C virus. Bound virus was detected by a colour assay based on the viral acetylesterase as described elsewhere (Schultze et al., I993). • Gangliosides. MDCK I and MDCK II cells were pretreated with sialidase from C. perfringens (1 U/ml) for I h at 37 °C. Following incubation with BBG ...
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Interferon



Interferons (IFNs) are a group of signaling proteins made and released by host cells in response to the presence of several pathogens, such as viruses, bacteria, parasites, and also tumor cells. In a typical scenario, a virus-infected cell will release interferons causing nearby cells to heighten their anti-viral defenses.IFNs belong to the large class of proteins known as cytokines, molecules used for communication between cells to trigger the protective defenses of the immune system that help eradicate pathogens. Interferons are named for their ability to ""interfere"" with viral replication by protecting cells from virus infections. IFNs also have various other functions: they activate immune cells, such as natural killer cells and macrophages; they increase host defenses by up-regulating antigen presentation by virtue of increasing the expression of major histocompatibility complex (MHC) antigens. Certain symptoms of infections, such as fever, muscle pain and ""flu-like symptoms"", are also caused by the production of IFNs and other cytokines.More than twenty distinct IFN genes and proteins have been identified in animals, including humans. They are typically divided among three classes: Type I IFN, Type II IFN, and Type III IFN. IFNs belonging to all three classes are important for fighting viral infections and for the regulation of the immune system.
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