Molecular characterisation of RecQ homologues in Arabidopsis
... a powerful tool for identifying putative homologous proteins by database searches with sequence motives of genes of known function from different organisms. Such a database search using TBLASTN with the conserved helicase motives of the Bloom and the Werner syndrome protein sequences of H.sapiens re ...
... a powerful tool for identifying putative homologous proteins by database searches with sequence motives of genes of known function from different organisms. Such a database search using TBLASTN with the conserved helicase motives of the Bloom and the Werner syndrome protein sequences of H.sapiens re ...
Final Exam 2012 - Med Study Group
... the inhibitor molecule may be chemically unrelated to the substrate. 35. If an enzyme solution is saturated with substrate, the most effective way to obtain an even faster yield of products is to • add more of the enzyme. • heat the solution to 90°C. • add more substrate. • add an allosteric inhibit ...
... the inhibitor molecule may be chemically unrelated to the substrate. 35. If an enzyme solution is saturated with substrate, the most effective way to obtain an even faster yield of products is to • add more of the enzyme. • heat the solution to 90°C. • add more substrate. • add an allosteric inhibit ...
Cybertory Manual (WP) - Attotron Biosensor Corporation
... phosphate groups of DNA make it an acid; they are highly negatively charged in aqueous solution at neutral pH. When negatively charged DNA molecules are placed in an electric field, they migrate toward the positive electrode. A matrix or gel placed in the path of the migrating DNA molecules can reta ...
... phosphate groups of DNA make it an acid; they are highly negatively charged in aqueous solution at neutral pH. When negatively charged DNA molecules are placed in an electric field, they migrate toward the positive electrode. A matrix or gel placed in the path of the migrating DNA molecules can reta ...
Sample newsletter January 2017
... Gel concentration also affects the movement of DNA fragments. There is a linear relationship between the logarithm of the mobility of the DNA and the gel concentration. By altering the agarose concentration it is possible to control the range of sizes of fragments that can be separated by electropho ...
... Gel concentration also affects the movement of DNA fragments. There is a linear relationship between the logarithm of the mobility of the DNA and the gel concentration. By altering the agarose concentration it is possible to control the range of sizes of fragments that can be separated by electropho ...
Lab 9
... In class we looked at pieces of DNA molecules, which were coded as sequences of bases: A, T, G, or C. We created a class called BSequence that read in a file, named the species and created a list of the base sequence. Recall that in a base sequence there may occur many genes and also gaps of junk wh ...
... In class we looked at pieces of DNA molecules, which were coded as sequences of bases: A, T, G, or C. We created a class called BSequence that read in a file, named the species and created a list of the base sequence. Recall that in a base sequence there may occur many genes and also gaps of junk wh ...
AUTOMATED DNA SEQUENCING, MegaBACE 1000
... fragment sizes from 25 base pairs up to 1000 bases are produced by the sequencing chemistry with each fragment containing a single fluorescent label that corresponds to the last DNA letter of the fragment and by matching the color to size the original DNA sequence can be easily determined. (Hawkins ...
... fragment sizes from 25 base pairs up to 1000 bases are produced by the sequencing chemistry with each fragment containing a single fluorescent label that corresponds to the last DNA letter of the fragment and by matching the color to size the original DNA sequence can be easily determined. (Hawkins ...
What is your DNA Alias
... different proteins have specific functions, such as making our hearts, hair, eyes, and ears. The building blocks of proteins are called amino acids. There are 20 amino acids. The order of DNA letters, determines the order of the amino acids, and therefore the type of protein that is made. Each amino ...
... different proteins have specific functions, such as making our hearts, hair, eyes, and ears. The building blocks of proteins are called amino acids. There are 20 amino acids. The order of DNA letters, determines the order of the amino acids, and therefore the type of protein that is made. Each amino ...
What is your DNA Alias
... different proteins have specific functions, such as making our hearts, hair, eyes, and ears. The building blocks of proteins are called amino acids. There are 20 amino acids. The order of DNA letters, determines the order of the amino acids, and therefore the type of protein that is made. Each amino ...
... different proteins have specific functions, such as making our hearts, hair, eyes, and ears. The building blocks of proteins are called amino acids. There are 20 amino acids. The order of DNA letters, determines the order of the amino acids, and therefore the type of protein that is made. Each amino ...
Molecular cloning, characterization, and homologous
... of chromosomal DNA and 3 units (1 μL) of Taq polymerase (Fermentas). PCR was carried out in 40 cycles: 1 min at 94 °C, 1 min at 55 °C, and 2 min at 72 °C. The initial denaturation was carried out at 94 °C for 3 min. The final extension was performed for 10 min at 72 °C. Reaction mixtures were run in ...
... of chromosomal DNA and 3 units (1 μL) of Taq polymerase (Fermentas). PCR was carried out in 40 cycles: 1 min at 94 °C, 1 min at 55 °C, and 2 min at 72 °C. The initial denaturation was carried out at 94 °C for 3 min. The final extension was performed for 10 min at 72 °C. Reaction mixtures were run in ...
DNA
... • The polymerase chain reaction, PCR, can produce many copies of a specific target segment of DNA • A three-step cycle—heating, cooling, and replication—brings about a chain reaction that produces an exponentially growing population of ...
... • The polymerase chain reaction, PCR, can produce many copies of a specific target segment of DNA • A three-step cycle—heating, cooling, and replication—brings about a chain reaction that produces an exponentially growing population of ...
Monitoring viral DNA release with capillary electrophoresis
... reveals extensive effort and time required for each sample preparation. In our work with T5 phages, we were able to avoid numerous microbiological techniques required to prepare the viral samples by using a commercial source of T5 phages. Capillary electrophoresis allowed rapid detection of commerci ...
... reveals extensive effort and time required for each sample preparation. In our work with T5 phages, we were able to avoid numerous microbiological techniques required to prepare the viral samples by using a commercial source of T5 phages. Capillary electrophoresis allowed rapid detection of commerci ...
Exam 3
... A) What are the values for the Vmax and Km in the absence and presence of inhibitor? Include units in your answer in order to receive full credit. B) What type of inhibitor is exhibited? C) A different inhibitor is known to act in a competitive fashion and when added at a concentration of 1mM causes ...
... A) What are the values for the Vmax and Km in the absence and presence of inhibitor? Include units in your answer in order to receive full credit. B) What type of inhibitor is exhibited? C) A different inhibitor is known to act in a competitive fashion and when added at a concentration of 1mM causes ...
Lecture3- Molecular Biology-1(2013).
... Function: Translation process (from mRNA to protein synthesis) It transfers amino acids to the growing protein chain ...
... Function: Translation process (from mRNA to protein synthesis) It transfers amino acids to the growing protein chain ...
Identification and Characterization of KLK-L4, a New Kallikrein
... this tissue) were selected for identification of the full structure of the gene. Different PCR reactions were performed using one reverse primer (L4-R1) together with each of the forward primers located in upstream exons that were found in the different EST clones (primers L4-B, L4-D, L4-E) (Table I ...
... this tissue) were selected for identification of the full structure of the gene. Different PCR reactions were performed using one reverse primer (L4-R1) together with each of the forward primers located in upstream exons that were found in the different EST clones (primers L4-B, L4-D, L4-E) (Table I ...
Real-time polymerase chain reaction
A real-time polymerase chain reaction is a laboratory technique of molecular biology based on the polymerase chain reaction (PCR). It monitors the amplification of a targeted DNA molecule during the PCR, i.e. in real-time, and not at its end, as in conventional PCR. Real-time PCR can be used quantitatively (Quantitative real-time PCR), semi-quantitatively, i.e. above/below a certain amount of DNA molecules (Semi quantitative real-time PCR) or qualitatively (Qualitative real-time PCR).Two common methods for the detection of PCR products in real-time PCR are: (1) non-specific fluorescent dyes that intercalate with any double-stranded DNA, and (2) sequence-specific DNA probes consisting of oligonucleotides that are labelled with a fluorescent reporter which permits detection only after hybridization of the probe with its complementary sequence.The Minimum Information for Publication of Quantitative Real-Time PCR Experiments (MIQE) guidelines propose that the abbreviation qPCR be used for quantitative real-time PCR and that RT-qPCR be used for reverse transcription–qPCR [1]. The acronym ""RT-PCR"" commonly denotes reverse transcription polymerase chain reaction and not real-time PCR, but not all authors adhere to this convention.