Characterization of the protein recognized by the monoclonal
... The objective of this study was to characterize low molecular weight proteins of B. burgdorferi sensu lato. Our main focus was a protein around 12 kDa, that is reactive with D6, a monoclonal antibody specific for B. garinii isolates. ...
... The objective of this study was to characterize low molecular weight proteins of B. burgdorferi sensu lato. Our main focus was a protein around 12 kDa, that is reactive with D6, a monoclonal antibody specific for B. garinii isolates. ...
Cloning - iGEM 2016
... A single colony was picked from LB-agar plate and inoculated into mini prep containing 10 mL of LB-medium and appropriate antibiotic for selection. ...
... A single colony was picked from LB-agar plate and inoculated into mini prep containing 10 mL of LB-medium and appropriate antibiotic for selection. ...
Chapter 13 Vocabulary Name
... 4. restriction enzyme: enzyme that cuts sugar-phosphate bonds in the DNA backbone at specific points within particular nucleotide sequences in DNA (Concept 13.2) 5. genomic library: complete collection of cloned DNA fragments from an organism (Concept 13.2) 6. nucleic acid probe: radioactively label ...
... 4. restriction enzyme: enzyme that cuts sugar-phosphate bonds in the DNA backbone at specific points within particular nucleotide sequences in DNA (Concept 13.2) 5. genomic library: complete collection of cloned DNA fragments from an organism (Concept 13.2) 6. nucleic acid probe: radioactively label ...
Discovery of Introns
... are read from the same nucleotides in the same reading frame (see chapter 13). How can it be a deletion and not be a deletion at the same time? In the human adenovirus (which causes the common cold), eight genes are transcribed late in the virus life cycle on one long RNA molecule accounting for mos ...
... are read from the same nucleotides in the same reading frame (see chapter 13). How can it be a deletion and not be a deletion at the same time? In the human adenovirus (which causes the common cold), eight genes are transcribed late in the virus life cycle on one long RNA molecule accounting for mos ...
Chapter 4 Cellular Metabolism
... process is going to depend on how many Carbons are in the piece the cell is working on. How many ATPs formed will also depend on this. Nucleic Acids: DNA & RNA ...
... process is going to depend on how many Carbons are in the piece the cell is working on. How many ATPs formed will also depend on this. Nucleic Acids: DNA & RNA ...
Answer any EIGHT questions from Section A. Each question carries
... DNA generated is itself used as a template for replication, setting in motion a chain reaction in which the DNA template is exponentially amplified. PCR can be extensively modified to perform a wide array of genetic manipulations. Almost all PCR applications employ a heat-stable DNA polymerase, such ...
... DNA generated is itself used as a template for replication, setting in motion a chain reaction in which the DNA template is exponentially amplified. PCR can be extensively modified to perform a wide array of genetic manipulations. Almost all PCR applications employ a heat-stable DNA polymerase, such ...
Chapter 8: Microbial Genetics 1. Gene Expression Gene Expression
... The lac repressor protein by default is bound to the operator sequence, thus blocking part of the promoter and preventing RNA polymerase from binding and initiating transcription of the lacZ, lacY & lacA genes. • the lac operon is OFF since there’s no need for these gene products in the absence of l ...
... The lac repressor protein by default is bound to the operator sequence, thus blocking part of the promoter and preventing RNA polymerase from binding and initiating transcription of the lacZ, lacY & lacA genes. • the lac operon is OFF since there’s no need for these gene products in the absence of l ...
DNA, RNA, and Proteins part 2 - Tri-City
... Instructions for making a protein are transferred ...
... Instructions for making a protein are transferred ...
Life on Mars
... experiments. A ‘positive control’ PCR, using a sample we know contains DNA is used to check that the PCR is working. A ‘negative control’, without DNA, is carried out to check that samples have not been contaminated during PCR preparation. Positive controls can also be used to exclude so-called “fal ...
... experiments. A ‘positive control’ PCR, using a sample we know contains DNA is used to check that the PCR is working. A ‘negative control’, without DNA, is carried out to check that samples have not been contaminated during PCR preparation. Positive controls can also be used to exclude so-called “fal ...
Transcription
... is red, and the non-template strand is yellow. The rudder wedges apart the DNA-RNA hybrid as the polymerase moves. For simplicity only the polypeptide backbone of the rudder is shown in the righthand figure, and the DNA exiting from the polymerase has been omitted. Because the RNA polymerase is depi ...
... is red, and the non-template strand is yellow. The rudder wedges apart the DNA-RNA hybrid as the polymerase moves. For simplicity only the polypeptide backbone of the rudder is shown in the righthand figure, and the DNA exiting from the polymerase has been omitted. Because the RNA polymerase is depi ...
Is the process of manipulating genes and genomes Biotechnology
... enzymes, the result will always be a set of restriction fragments which will have at least one single-stranded end, called a sticky-end -Sticky ends can form hydrogen bonds with complementary single-stranded-pieces of DNA these unions can be sealed with the enzyme DNA ligase -Is DNA that has been ar ...
... enzymes, the result will always be a set of restriction fragments which will have at least one single-stranded end, called a sticky-end -Sticky ends can form hydrogen bonds with complementary single-stranded-pieces of DNA these unions can be sealed with the enzyme DNA ligase -Is DNA that has been ar ...
proteins
... Genetic code: table that gives the correspondence between each possible triplet and each amino acid ...
... Genetic code: table that gives the correspondence between each possible triplet and each amino acid ...
RTP DNA/RNA Virus Mini Kit
... Virus RNA was isolated from 200 µl HCV infected human serum sample using the RTP® DNA/RNA Virus Mini Kit (serum contain 50.000 HCV copies/ml, detected by Cobas Amplicor Monitor® HCV Roche, Mannheim). 5 µl out of 60 µl of the eluted virus RNA was used in a in-house HCV-RT-PCR (EZ buffer/rTth). The RT ...
... Virus RNA was isolated from 200 µl HCV infected human serum sample using the RTP® DNA/RNA Virus Mini Kit (serum contain 50.000 HCV copies/ml, detected by Cobas Amplicor Monitor® HCV Roche, Mannheim). 5 µl out of 60 µl of the eluted virus RNA was used in a in-house HCV-RT-PCR (EZ buffer/rTth). The RT ...
More Exam Practice - Iowa State University
... 1.Describe where the light reactions and the calvin cycle take place in a plant cell and what the inputs and outputs are of the two stages. The light reactions occur in the thylakoid membranes of chloroplasts. The inputs are H2O and light energy and the outputs are NADPH, ATP, and oxygen. The calvi ...
... 1.Describe where the light reactions and the calvin cycle take place in a plant cell and what the inputs and outputs are of the two stages. The light reactions occur in the thylakoid membranes of chloroplasts. The inputs are H2O and light energy and the outputs are NADPH, ATP, and oxygen. The calvi ...
A. thaliana genotyping with a CAPS marker for a pks3
... 3 fragments: 29 bp, 182 bp, and 307 bp. The pks3-7 mutant sequence is missing an MboI restriction site, and digestion of the mutant 518 bp fragment yielded 2 fragments: 211 bp and 307 bp. Digested samples were analyzed using the QIAxcel capillary electrophoresis system with the QIAxcel DNA Screening ...
... 3 fragments: 29 bp, 182 bp, and 307 bp. The pks3-7 mutant sequence is missing an MboI restriction site, and digestion of the mutant 518 bp fragment yielded 2 fragments: 211 bp and 307 bp. Digested samples were analyzed using the QIAxcel capillary electrophoresis system with the QIAxcel DNA Screening ...
Lecture 4: DNA transcription
... Gene expression efficiency- How is transcription controlled? E.g. When to transcribe gene? How many copies to be transcribed? DNA binding proteins usually regulate transcriptional activity. These are proteins that recognise & bind to specific sequences on DNA. Recognition is determined by specific s ...
... Gene expression efficiency- How is transcription controlled? E.g. When to transcribe gene? How many copies to be transcribed? DNA binding proteins usually regulate transcriptional activity. These are proteins that recognise & bind to specific sequences on DNA. Recognition is determined by specific s ...
Real-time polymerase chain reaction
A real-time polymerase chain reaction is a laboratory technique of molecular biology based on the polymerase chain reaction (PCR). It monitors the amplification of a targeted DNA molecule during the PCR, i.e. in real-time, and not at its end, as in conventional PCR. Real-time PCR can be used quantitatively (Quantitative real-time PCR), semi-quantitatively, i.e. above/below a certain amount of DNA molecules (Semi quantitative real-time PCR) or qualitatively (Qualitative real-time PCR).Two common methods for the detection of PCR products in real-time PCR are: (1) non-specific fluorescent dyes that intercalate with any double-stranded DNA, and (2) sequence-specific DNA probes consisting of oligonucleotides that are labelled with a fluorescent reporter which permits detection only after hybridization of the probe with its complementary sequence.The Minimum Information for Publication of Quantitative Real-Time PCR Experiments (MIQE) guidelines propose that the abbreviation qPCR be used for quantitative real-time PCR and that RT-qPCR be used for reverse transcription–qPCR [1]. The acronym ""RT-PCR"" commonly denotes reverse transcription polymerase chain reaction and not real-time PCR, but not all authors adhere to this convention.