Gene Linkage
... – Plasmid: A small, circular DNA molecule in bacterial cells that is separate from the bacteria’s chromosome. ...
... – Plasmid: A small, circular DNA molecule in bacterial cells that is separate from the bacteria’s chromosome. ...
Lab #5a Mr. Green Genes-DNA Sequence
... to identify genes and other functional or evolutionarily significant sequences is a critical for the 21st century biologist. In this exercise, we will learn some of the fundamental techniques of the rapidly expanding field of bioinformatics. We will begin by identifying open reading frames within th ...
... to identify genes and other functional or evolutionarily significant sequences is a critical for the 21st century biologist. In this exercise, we will learn some of the fundamental techniques of the rapidly expanding field of bioinformatics. We will begin by identifying open reading frames within th ...
emboj2008205-sup
... of expansions and contractions of repeat tracts during mitotic divisions, we re-streaked yeast colonies that have been verified for the presence of (GAA)340 full size repeats on complete media. Ten colonies were then selected for PCR amplification to look for changes in the length of the repetitive ...
... of expansions and contractions of repeat tracts during mitotic divisions, we re-streaked yeast colonies that have been verified for the presence of (GAA)340 full size repeats on complete media. Ten colonies were then selected for PCR amplification to look for changes in the length of the repetitive ...
Practical Molecular Biology and Genetic Engineering
... Craig Venter's 1st synthetic cell had neither synthetic cytoplasm nor synthetic DNA, only the DNA fragments that were assembled by a yeast cell in a multistep process were synthetic. The NIH originally wanted to confine all recombinant DNA technology to class 3 facilities. A company has produced a D ...
... Craig Venter's 1st synthetic cell had neither synthetic cytoplasm nor synthetic DNA, only the DNA fragments that were assembled by a yeast cell in a multistep process were synthetic. The NIH originally wanted to confine all recombinant DNA technology to class 3 facilities. A company has produced a D ...
Bio 102 Practice Problems
... EcoRI and BamHI. She then obtains a cloning vector and digests it with the same two enzymes. She then runs a gel, which is shown at the right. a. Which enzyme would she want to use for cloning the potato DNA: EcoRI, or BamHI? Explain why you made your choice. b. Notice that the cloning vector made n ...
... EcoRI and BamHI. She then obtains a cloning vector and digests it with the same two enzymes. She then runs a gel, which is shown at the right. a. Which enzyme would she want to use for cloning the potato DNA: EcoRI, or BamHI? Explain why you made your choice. b. Notice that the cloning vector made n ...
Bio-Rad pGLO kit
... a powerful tool wherein DNA from one organism is added to the DNA of a different species. The results of genetic modification in agriculture have resulted in plants that can make their own pesticides and resist herbicides. In this lesson, green fluorescent protein (GFP) is inserted or added to non-v ...
... a powerful tool wherein DNA from one organism is added to the DNA of a different species. The results of genetic modification in agriculture have resulted in plants that can make their own pesticides and resist herbicides. In this lesson, green fluorescent protein (GFP) is inserted or added to non-v ...
Automating the Promega Wizard® SV 96 Plasmid DNA Purification
... Plasmid DNA is useful in a wide range of molecular biology applications. However, purification of plasmid DNA from pelleted bacterial culture can be time-consuming when done manually. Automation of this process can deliver significant increases in throughput as well as higher levels of precision and ...
... Plasmid DNA is useful in a wide range of molecular biology applications. However, purification of plasmid DNA from pelleted bacterial culture can be time-consuming when done manually. Automation of this process can deliver significant increases in throughput as well as higher levels of precision and ...
Final
... parenthesis that most accurately completes the statement. (1 point each). The study of variation in bacteria has several features that are distinct from the study of genetics in eukaryotic organisms. Bacteria typically have (a single, two, multiple) chromosome(s) that is(are) composed of (single str ...
... parenthesis that most accurately completes the statement. (1 point each). The study of variation in bacteria has several features that are distinct from the study of genetics in eukaryotic organisms. Bacteria typically have (a single, two, multiple) chromosome(s) that is(are) composed of (single str ...
Multiple Mechanisms Contribute to Lateral Transfer of an
... used for tagging with minitransposon EZ-Tn5,R6Kgori/KAN-2. using the EZTn5 ,R6Kgori/KAN-2. insertion kit (Epicenter Biotechnologies, USA) following the manufacturer’s protocols. The isolated plasmid preparation and minitransposon was taken in equimolar concentrations and incubated with transposase f ...
... used for tagging with minitransposon EZ-Tn5,R6Kgori/KAN-2. using the EZTn5 ,R6Kgori/KAN-2. insertion kit (Epicenter Biotechnologies, USA) following the manufacturer’s protocols. The isolated plasmid preparation and minitransposon was taken in equimolar concentrations and incubated with transposase f ...
Lab 5 minipreps
... proteins that recognize and bind to specific DNA sequences and cut the DNA at or near the recognition site. Restriction enzymes were originally discovered through their ability to break down, or "restrict" foreign DNA. In their natural environment, the bacterial cell, they serve a protective functio ...
... proteins that recognize and bind to specific DNA sequences and cut the DNA at or near the recognition site. Restriction enzymes were originally discovered through their ability to break down, or "restrict" foreign DNA. In their natural environment, the bacterial cell, they serve a protective functio ...
Environmental reservoirs of antibiotic resistance genes
... • CTX-M-15 is carried throughout a wide range of genetic contexts and plasmids • Contexts were seen in human pathogens, including several novel genetic contexts ...
... • CTX-M-15 is carried throughout a wide range of genetic contexts and plasmids • Contexts were seen in human pathogens, including several novel genetic contexts ...
Document
... • Found in most bacterial cells. • Self-replicating, extra chromosomal DNA. • Closed, circular, double-stranded. • Smaller than chromosomal DNA with only 3,000-30,000 base pairs. • Contain information for translation of specialized and protective proteins. ...
... • Found in most bacterial cells. • Self-replicating, extra chromosomal DNA. • Closed, circular, double-stranded. • Smaller than chromosomal DNA with only 3,000-30,000 base pairs. • Contain information for translation of specialized and protective proteins. ...
enzymes and vectors
... SHUTTLE VECTORS • Possess two origin for replication (ori E & ori Euk). • Can be expressed in either host • Can be grown in one host and shifted to another host • ori E functions in E. coli & ori Euk functions in eukaryotic cells like yeast. ...
... SHUTTLE VECTORS • Possess two origin for replication (ori E & ori Euk). • Can be expressed in either host • Can be grown in one host and shifted to another host • ori E functions in E. coli & ori Euk functions in eukaryotic cells like yeast. ...
Lab 10 Study Guide
... Plasmids are double stranded circular DNA that exist in addition to the single chromosome in bacteria. They carry genes that may be beneficial under environmental stress allowing cells to adapt very quickly to a changing environment. This causes evolution to occur over very short period of time. ...
... Plasmids are double stranded circular DNA that exist in addition to the single chromosome in bacteria. They carry genes that may be beneficial under environmental stress allowing cells to adapt very quickly to a changing environment. This causes evolution to occur over very short period of time. ...
Yeast Transformation
... 7. Remove 10 µL of the resuspended cells to 90 µL of sterile water in a microcentrifuge tube. This sample will be serially diluted for a spot plate (step 10) that you will use to calculate the transformation efficiency. 8. Plate the remainder of the mixture on a selective media lacking uracil. ...
... 7. Remove 10 µL of the resuspended cells to 90 µL of sterile water in a microcentrifuge tube. This sample will be serially diluted for a spot plate (step 10) that you will use to calculate the transformation efficiency. 8. Plate the remainder of the mixture on a selective media lacking uracil. ...
lab 10 dna transformation student guide
... initiation site of DNA replication in E. coli. It must be present or the plasmid DNA will not be replicated. Bluescript is a high copy number plasmid, which means that replication occurs repeatedly until as many as 500-700 plasmids are present within the cell. The gene for chloramphenicol resistance ...
... initiation site of DNA replication in E. coli. It must be present or the plasmid DNA will not be replicated. Bluescript is a high copy number plasmid, which means that replication occurs repeatedly until as many as 500-700 plasmids are present within the cell. The gene for chloramphenicol resistance ...
Hybrid Plasmids Containing the Pyruvate
... strain were not significantly amplified suggesting that expression of the ace and lpd genes could be tightly coupled to metabolic need (despite a high copy number) or that the average plasmid copy number is itself not significantly greater than one. In the case of pGS5 the elevated lipoamide dehydro ...
... strain were not significantly amplified suggesting that expression of the ace and lpd genes could be tightly coupled to metabolic need (despite a high copy number) or that the average plasmid copy number is itself not significantly greater than one. In the case of pGS5 the elevated lipoamide dehydro ...
S1 Supporting Information
... The amplified 5.1 kb fragment was BglII digested and ligated with the 3.4 kb pyrG fragment to give plasmid pMAT768. A 5.5 kb replacement fragment harboring the pyrG gene flanked by 1.1 kb and 1.0 kb of sequences adjacent to 136157 was released from plasmid pMAT768 by PvuII digestion, amplified with ...
... The amplified 5.1 kb fragment was BglII digested and ligated with the 3.4 kb pyrG fragment to give plasmid pMAT768. A 5.5 kb replacement fragment harboring the pyrG gene flanked by 1.1 kb and 1.0 kb of sequences adjacent to 136157 was released from plasmid pMAT768 by PvuII digestion, amplified with ...
in no vatio ns fo ru m - GE Healthcare Life Sciences
... All the isolated plasmid DNA samples were of sufficient quality (irrespective of the purification kit used) to facilitate the amplification of a 1187-bp product (Fig 4). Comparable band intensities were observed for each individual DNA polymerase when we compared QIAprep to illustra plasmid DNA templat ...
... All the isolated plasmid DNA samples were of sufficient quality (irrespective of the purification kit used) to facilitate the amplification of a 1187-bp product (Fig 4). Comparable band intensities were observed for each individual DNA polymerase when we compared QIAprep to illustra plasmid DNA templat ...
基因定点整合
... transgenes derived from different sources typically integrate at different locations in the plant genome, which may lead to various expression patterns and possible segregation of the transgenes in the offspring. ...
... transgenes derived from different sources typically integrate at different locations in the plant genome, which may lead to various expression patterns and possible segregation of the transgenes in the offspring. ...
Schedule of Lecture and Laboratory Sessions
... Define: prototroph, auxotroph, minimal, selective, and complete media Contrast nutritional, conditional, and resistance mutations in bacteria Describe parasexual mating (conjugation) between F+ and F- bacteria including role of pilus Explain the F factor, what it encodes, and the mechanism of transf ...
... Define: prototroph, auxotroph, minimal, selective, and complete media Contrast nutritional, conditional, and resistance mutations in bacteria Describe parasexual mating (conjugation) between F+ and F- bacteria including role of pilus Explain the F factor, what it encodes, and the mechanism of transf ...
April 8
... T-DNA contains “oncogenic genes” that cause overproduction of auxin and cytokinin: cause transformed cells to form tumors Also have gene forcing cell to make opines: funny amino acids that only Agro can use: convert host into factory feeding Agro! Plant mol biologists have “disarmed” the Ti plasmid ...
... T-DNA contains “oncogenic genes” that cause overproduction of auxin and cytokinin: cause transformed cells to form tumors Also have gene forcing cell to make opines: funny amino acids that only Agro can use: convert host into factory feeding Agro! Plant mol biologists have “disarmed” the Ti plasmid ...
Manipulating Yeast Genome Using Plasmid Vectors. In: Gene Expression Technology.
... pBR322. 6 This plasmid has a bacterial replication origin that allows maintenance at high copy number, and two selectable antibiotic resistance genes. The bla gene encodes resistance to the fl-lactam ampicillin, and the tet gene encodes resistance to tetracycline. In addition, pBR322 has a number of ...
... pBR322. 6 This plasmid has a bacterial replication origin that allows maintenance at high copy number, and two selectable antibiotic resistance genes. The bla gene encodes resistance to the fl-lactam ampicillin, and the tet gene encodes resistance to tetracycline. In addition, pBR322 has a number of ...
In vitro conjugal transfer of tetracycline resistance from Lactobacillus
... include the genera Pediococcus, Enterococcus, Lactobacillus and Leuconostoc but not the genus Lactococcus [23]. Our ¢ndings indicate that the R-plasmids of the investigated Tcr Lactobacillus strains have di¡erent conjugation abilities: some plasmids were transferable to the genera Enterococcus and L ...
... include the genera Pediococcus, Enterococcus, Lactobacillus and Leuconostoc but not the genus Lactococcus [23]. Our ¢ndings indicate that the R-plasmids of the investigated Tcr Lactobacillus strains have di¡erent conjugation abilities: some plasmids were transferable to the genera Enterococcus and L ...
Recombinant DNA - Rose
... Plasmid preparation procedures are non-specific: they can be used to purify any plasmid present within the bacteria. This is a major advantage, because it means that the protocol does not need to be changed for different plasmids. However, it also means that it is possible to purify the wrong plasm ...
... Plasmid preparation procedures are non-specific: they can be used to purify any plasmid present within the bacteria. This is a major advantage, because it means that the protocol does not need to be changed for different plasmids. However, it also means that it is possible to purify the wrong plasm ...
Plasmid
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A plasmid is a small DNA molecule within a cell that is physically separated from a chromosomal DNA and can replicate independently. They are most commonly found in bacteria as small, circular, double-stranded DNA molecules; however, plasmids are sometimes present in archaea and eukaryotic organisms. In nature, plasmids often carry genes that may benefit the survival of the organism, for example antibiotic resistance. While the chromosomes are big and contain all the essential information for living, plasmids usually are very small and contain only additional information. Artificial plasmids are widely used as vectors in molecular cloning, serving to drive the replication of recombinant DNA sequences within host organisms.Plasmids are considered replicons, a unit of DNA capable of replicating autonomously within a suitable host. However, plasmids, like viruses, are not generally classified as life. Plasmids can be transmitted from one bacterium to another (even of another species) via three main mechanisms: transformation, transduction, and conjugation. This host-to-host transfer of genetic material is called horizontal gene transfer, and plasmids can be considered part of the mobilome. Unlike viruses (which encase their genetic material in a protective protein coat called a capsid), plasmids are ""naked"" DNA and do not encode genes necessary to encase the genetic material for transfer to a new host. However, some classes of plasmids encode the conjugative ""sex"" pilus necessary for their own transfer. The size of the plasmid varies from 1 to over 200 kbp, and the number of identical plasmids in a single cell can range anywhere from one to thousands under some circumstances.The relationship between microbes and plasmid DNA is neither parasitic nor mutualistic, because each implies the presence of an independent species living in a detrimental or commensal state with the host organism. Rather, plasmids provide a mechanism for horizontal gene transfer within a population of microbes and typically provide a selective advantage under a given environmental state. Plasmids may carry genes that provide resistance to naturally occurring antibiotics in a competitive environmental niche, or the proteins produced may act as toxins under similar circumstances, or allow the organism to utilize particular organic compounds that would be advantageous when nutrients are scarce.