2009 exam 3
... A. The initiator tRNA could be in (the P site) (the A site) (the E site) (A or P) (A or E) (E or P) (any of these). B. Methionine should be attached directly to (tRNA #1) (AA #2 = amino acid #2) (tRNA #2) (AA #3) (peptidyl transferase) (either tRNA) (tRNA or AA #2) (either AA) (none of these) (any o ...
... A. The initiator tRNA could be in (the P site) (the A site) (the E site) (A or P) (A or E) (E or P) (any of these). B. Methionine should be attached directly to (tRNA #1) (AA #2 = amino acid #2) (tRNA #2) (AA #3) (peptidyl transferase) (either tRNA) (tRNA or AA #2) (either AA) (none of these) (any o ...
Lab 7: Molecular Biology
... these chromosomes. Although these circular DNA molecules are usually broken into linear fragments during purification, some bacteria also possess smaller extrachromosomal circular DNA molecules that are easier to isolate without breaking. These extrachromosomal DNA molecules are called plasmids and ...
... these chromosomes. Although these circular DNA molecules are usually broken into linear fragments during purification, some bacteria also possess smaller extrachromosomal circular DNA molecules that are easier to isolate without breaking. These extrachromosomal DNA molecules are called plasmids and ...
Supplemental Data Whorl-Specific Expression of
... Plasmid Construction All reporter genes were constructed by standard molecular biology techniques. All constructs, except for #3, were based on pSUP-GUS (BsaBI), which was made by inserting a uidA-coding region in the BsaBI site (8243094 of chromosome 3; blunt end, position 1848 of TAC clone K14B15 ...
... Plasmid Construction All reporter genes were constructed by standard molecular biology techniques. All constructs, except for #3, were based on pSUP-GUS (BsaBI), which was made by inserting a uidA-coding region in the BsaBI site (8243094 of chromosome 3; blunt end, position 1848 of TAC clone K14B15 ...
No Slide Title
... • DNA from the organism of interest is divided into small pieces that are then placed into individual cells (usually bacterial). • These can then be separated as individual colonies on plates, and they can be screened to find the gene of interest. • This process is also called molecular cloning. ...
... • DNA from the organism of interest is divided into small pieces that are then placed into individual cells (usually bacterial). • These can then be separated as individual colonies on plates, and they can be screened to find the gene of interest. • This process is also called molecular cloning. ...
pDsRed-Express-1 Vector
... different promoters and promoter/enhancer combinations inserted into the multiple cloning site (MCS). It encodes DsRedExpress, a variant of Discosoma sp. red fluorescent protein (DsRed; 1). DsRed-Express contains nine amino acid substitutions which improve the solubility of the protein, reduce the t ...
... different promoters and promoter/enhancer combinations inserted into the multiple cloning site (MCS). It encodes DsRedExpress, a variant of Discosoma sp. red fluorescent protein (DsRed; 1). DsRed-Express contains nine amino acid substitutions which improve the solubility of the protein, reduce the t ...
Bacterial transformation - BLI-Research-Synbio-2014-session-1
... DNA fragments can be joined easily together. • When DNA from two sources is joined together, the enzyme DNA ligase is used to catalyze bonding between sugar and phosphate groups in the DNA backbone. • DNA from a “foreign” source (plant, animal, viral, bacterial, yeast) is generally bonded to vector ...
... DNA fragments can be joined easily together. • When DNA from two sources is joined together, the enzyme DNA ligase is used to catalyze bonding between sugar and phosphate groups in the DNA backbone. • DNA from a “foreign” source (plant, animal, viral, bacterial, yeast) is generally bonded to vector ...
Unit 4 Cram Sheet
... Creating a human protein must begin with finding something to “grow” that protein. The easiest living organism to use for this purpose is bacteria. This involves using recombinant DNA technology to custom-design bacteria that can produce the proteins we want. This process has several steps, which wi ...
... Creating a human protein must begin with finding something to “grow” that protein. The easiest living organism to use for this purpose is bacteria. This involves using recombinant DNA technology to custom-design bacteria that can produce the proteins we want. This process has several steps, which wi ...
Practice exam 3 key
... pUC map (4 pts) -1 pt for each error in site or fragment length; 1 pt if just the 3 sites shown IF relative spacing approximately correct. Plasmids X and Y (8 pts): vector fragments correct (3 pts); yfg/EcoRI fragment = 1 kb (1 pt); BamHI site subdivides yfg/EcoRI fragment into 0.2 kb and 0.8 kb (2 ...
... pUC map (4 pts) -1 pt for each error in site or fragment length; 1 pt if just the 3 sites shown IF relative spacing approximately correct. Plasmids X and Y (8 pts): vector fragments correct (3 pts); yfg/EcoRI fragment = 1 kb (1 pt); BamHI site subdivides yfg/EcoRI fragment into 0.2 kb and 0.8 kb (2 ...
Construction and stable transformation of Tetrahymena
... main goals of Tetrahymena laboratories will be characterizing the unknown biological function of these genes in lifecycle by using functional complementation analysis based on phenotype with transfering genomic DNA library into mutant Tetrahymena cells using AC based vectors. There is no published l ...
... main goals of Tetrahymena laboratories will be characterizing the unknown biological function of these genes in lifecycle by using functional complementation analysis based on phenotype with transfering genomic DNA library into mutant Tetrahymena cells using AC based vectors. There is no published l ...
crispr - UNM Biology
... byproduct. However, The spacer between each repeat varied between organisms. • Jansen et al, 2002 Four Cas genes near the repeats with helicase, and nuclease protein domains. • Together coined the term CRISPR: Clustered Regularly Interspaced Short Palindromic Repeats describing the loci ...
... byproduct. However, The spacer between each repeat varied between organisms. • Jansen et al, 2002 Four Cas genes near the repeats with helicase, and nuclease protein domains. • Together coined the term CRISPR: Clustered Regularly Interspaced Short Palindromic Repeats describing the loci ...
Design, synthesis and screening of potential
... action of metallo- -lactamases capable of hydrolysing the -lactam rings found in antibiotics such as penicillins, cephalosporins and carbapenems. The most recent member of the family wields the particular enzyme New Delhi Metallo- -lactamase (NDM-1). This monomeric enzyme has a molecular mass of ...
... action of metallo- -lactamases capable of hydrolysing the -lactam rings found in antibiotics such as penicillins, cephalosporins and carbapenems. The most recent member of the family wields the particular enzyme New Delhi Metallo- -lactamase (NDM-1). This monomeric enzyme has a molecular mass of ...
Recombinant DNA Technology
... What are the differences between a plasmid and a chromosome? A plasmid is a circle of DNA that comes from bacterial cells. Many of them contain genes for antibiotic resistance. Chromosomal DNA is linear DNA. (Human DNA contains both introns and exons whereas plasmid DNA does not contain introns.) Sc ...
... What are the differences between a plasmid and a chromosome? A plasmid is a circle of DNA that comes from bacterial cells. Many of them contain genes for antibiotic resistance. Chromosomal DNA is linear DNA. (Human DNA contains both introns and exons whereas plasmid DNA does not contain introns.) Sc ...
Exam #3 Review
... strands of DNA can always serve as the template for the synthesis of the other strand. c. the hydrogen bonds holding the strands of nucleotides together can be broken in a process called denaturation or melting. d. all of the above. Practice: In what ways is RNA different than DNA? B. DNA can be tra ...
... strands of DNA can always serve as the template for the synthesis of the other strand. c. the hydrogen bonds holding the strands of nucleotides together can be broken in a process called denaturation or melting. d. all of the above. Practice: In what ways is RNA different than DNA? B. DNA can be tra ...
For Official Use ENV/JM/BIO(2006)6/REV3 Working
... Circular or linear extrachromosomal replicons; self-transferable or mobilisable plasmids are vehicles for the transmission of genetic information between a broad or narrow range of species Viruses that infect prokaryotes; can integrate into the host genome and then be vehicles for horizontal gene tr ...
... Circular or linear extrachromosomal replicons; self-transferable or mobilisable plasmids are vehicles for the transmission of genetic information between a broad or narrow range of species Viruses that infect prokaryotes; can integrate into the host genome and then be vehicles for horizontal gene tr ...
pGLO Transformation Lab Introduction to Transformation In this lab
... fluorescent protein which causes them to glow a brilliant green color under ultraviolet light. In this activity, you will learn about the process of moving genes from one organism to another with the aid of a plasmid. In addition to one large chromosome, bacteria naturally contain one or more small ...
... fluorescent protein which causes them to glow a brilliant green color under ultraviolet light. In this activity, you will learn about the process of moving genes from one organism to another with the aid of a plasmid. In addition to one large chromosome, bacteria naturally contain one or more small ...
Botany LBC-101 Unit-II lect
... cytoplasm. They consist of ribosomal RNA (rRNA). Their DNA contains a single, circular molecule, which is compact and tightly wound. No protein is associated with DNA. 7. The archaebacterial cell may contain plasmids, which are small, circular pieces of DNA. They can duplicate independent of a large ...
... cytoplasm. They consist of ribosomal RNA (rRNA). Their DNA contains a single, circular molecule, which is compact and tightly wound. No protein is associated with DNA. 7. The archaebacterial cell may contain plasmids, which are small, circular pieces of DNA. They can duplicate independent of a large ...
Transformation of Clostridium Thermocellum by Electroporation
... orientation of the 50 –30 flanks and the int region affects the resulting intermediate chromosomal configuration. If the orientation of the flanks is the same as that shown in Fig. 17.3 (i.e., the order is 50 flank, 30 flank, cat, hpt, and int region), the intermediate chromosomal configuration will ...
... orientation of the 50 –30 flanks and the int region affects the resulting intermediate chromosomal configuration. If the orientation of the flanks is the same as that shown in Fig. 17.3 (i.e., the order is 50 flank, 30 flank, cat, hpt, and int region), the intermediate chromosomal configuration will ...
Chapter 20~ DNA Technology & Genomics
... Single circular chromosome ◦ haploid ◦ naked DNA no histone proteins ...
... Single circular chromosome ◦ haploid ◦ naked DNA no histone proteins ...
Biotechnology and Recombinant DNA I. Tools of Biotechnology
... • phage/viruses • Cosmid • BACs – bacterial artificial chromosomes/YACs – yeast artificial chromosomes – linear plasmids Vector features 1. Origin of replication (ori) and other replication functions for stable maintenance in host cells. Shuttle vector: can replicate in several different species (e. ...
... • phage/viruses • Cosmid • BACs – bacterial artificial chromosomes/YACs – yeast artificial chromosomes – linear plasmids Vector features 1. Origin of replication (ori) and other replication functions for stable maintenance in host cells. Shuttle vector: can replicate in several different species (e. ...
CHAPTER 18 OBJECTIVES-BACTERIAL GENOME The Genetics of
... 2. Describe the natural function of restriction enzymes and explain how they are used in recombinant DNA technology. 3. Explain how the creation of sticky ends by restriction enzymes is useful in producing a recombinant DNA molecule. 4. Outline the procedures for cloning a eukaryotic gene in a bacte ...
... 2. Describe the natural function of restriction enzymes and explain how they are used in recombinant DNA technology. 3. Explain how the creation of sticky ends by restriction enzymes is useful in producing a recombinant DNA molecule. 4. Outline the procedures for cloning a eukaryotic gene in a bacte ...
Recombinant DNA Technology
... What are the differences between a plasmid and a chromosome? A plasmid is a circle of DNA that comes from bacterial cells. Many of them contain genes for antibiotic resistance. Chromosomal DNA is linear DNA. (Human DNA contains both introns and exons whereas plasmid DNA does not contain introns.) Sc ...
... What are the differences between a plasmid and a chromosome? A plasmid is a circle of DNA that comes from bacterial cells. Many of them contain genes for antibiotic resistance. Chromosomal DNA is linear DNA. (Human DNA contains both introns and exons whereas plasmid DNA does not contain introns.) Sc ...
PowerPoint 演示文稿
... The pili make specific contact with a receptor on the recipient and then retract, pulling the two cells together. The contacts between the donor and recipient cells then become stabilized, probably from fusion of the outer membranes, and the DNA is then transferred from one cell to another. ...
... The pili make specific contact with a receptor on the recipient and then retract, pulling the two cells together. The contacts between the donor and recipient cells then become stabilized, probably from fusion of the outer membranes, and the DNA is then transferred from one cell to another. ...
Chapter 13 Presentation-Meiosis and Chromosomes
... organism to the next within a species. They are the vehicles of heredity. Minor differences in the sequences of base pairs on these chromosomes is what contributes to variation. ...
... organism to the next within a species. They are the vehicles of heredity. Minor differences in the sequences of base pairs on these chromosomes is what contributes to variation. ...
Chapter 2
... peptidoglycan, whereas gram-negative bacteria do not have teichoic acids. 2. The gram-negative bacteria have a complex outer layer consisting of lipopolysaccharide, lipoprotein, and phospholipid. ● Lying between the outer-membrane layer and the cytoplasmic membrane in gramnegative bacteria is the pe ...
... peptidoglycan, whereas gram-negative bacteria do not have teichoic acids. 2. The gram-negative bacteria have a complex outer layer consisting of lipopolysaccharide, lipoprotein, and phospholipid. ● Lying between the outer-membrane layer and the cytoplasmic membrane in gramnegative bacteria is the pe ...
Plasmid
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A plasmid is a small DNA molecule within a cell that is physically separated from a chromosomal DNA and can replicate independently. They are most commonly found in bacteria as small, circular, double-stranded DNA molecules; however, plasmids are sometimes present in archaea and eukaryotic organisms. In nature, plasmids often carry genes that may benefit the survival of the organism, for example antibiotic resistance. While the chromosomes are big and contain all the essential information for living, plasmids usually are very small and contain only additional information. Artificial plasmids are widely used as vectors in molecular cloning, serving to drive the replication of recombinant DNA sequences within host organisms.Plasmids are considered replicons, a unit of DNA capable of replicating autonomously within a suitable host. However, plasmids, like viruses, are not generally classified as life. Plasmids can be transmitted from one bacterium to another (even of another species) via three main mechanisms: transformation, transduction, and conjugation. This host-to-host transfer of genetic material is called horizontal gene transfer, and plasmids can be considered part of the mobilome. Unlike viruses (which encase their genetic material in a protective protein coat called a capsid), plasmids are ""naked"" DNA and do not encode genes necessary to encase the genetic material for transfer to a new host. However, some classes of plasmids encode the conjugative ""sex"" pilus necessary for their own transfer. The size of the plasmid varies from 1 to over 200 kbp, and the number of identical plasmids in a single cell can range anywhere from one to thousands under some circumstances.The relationship between microbes and plasmid DNA is neither parasitic nor mutualistic, because each implies the presence of an independent species living in a detrimental or commensal state with the host organism. Rather, plasmids provide a mechanism for horizontal gene transfer within a population of microbes and typically provide a selective advantage under a given environmental state. Plasmids may carry genes that provide resistance to naturally occurring antibiotics in a competitive environmental niche, or the proteins produced may act as toxins under similar circumstances, or allow the organism to utilize particular organic compounds that would be advantageous when nutrients are scarce.