Transformation Lab - Towson University
... encoded in the foreign DNA. Transformation enables inexpensive and reliable production of important medical products such as insulin, human growth hormone, and other replacement hormone and gene therapies. In bacteria, the haploid genome is a single circular chromosome. This differs from eukaryotic ...
... encoded in the foreign DNA. Transformation enables inexpensive and reliable production of important medical products such as insulin, human growth hormone, and other replacement hormone and gene therapies. In bacteria, the haploid genome is a single circular chromosome. This differs from eukaryotic ...
12_Lecture_Presentation - Cornerstone Charter Academy
... 12.1 Genes can be cloned in recombinant plasmids Steps in cloning a gene 1. Plasmid DNA is isolated 2. DNA containing the gene of interest is isolated 3. Plasmid DNA is treated with restriction enzyme that cuts in one place, opening the circle ...
... 12.1 Genes can be cloned in recombinant plasmids Steps in cloning a gene 1. Plasmid DNA is isolated 2. DNA containing the gene of interest is isolated 3. Plasmid DNA is treated with restriction enzyme that cuts in one place, opening the circle ...
F plasmid
... • A piece of DNA called the F factor is required for the production of sex pili • The F factor can exist as a separate plasmid or as DNA within the bacterial chromosome Copyright © 2008 Pearson Education, Inc., publishing as Pearson Benjamin Cummings ...
... • A piece of DNA called the F factor is required for the production of sex pili • The F factor can exist as a separate plasmid or as DNA within the bacterial chromosome Copyright © 2008 Pearson Education, Inc., publishing as Pearson Benjamin Cummings ...
The Amino Terminus of the Yeast F1-ATPase {j
... Plasmid pSEY303 contains a truncated form of the yeast SUC2 gene. The SUC2 gene has been cloned (3) and its entire nucleotide sequence has been determined (34). SUC2 contains a single Bam HI site at codon 263 in its coding sequence. This site was eliminated by sodium bisulfite mutagenesis. 2 l'g of ...
... Plasmid pSEY303 contains a truncated form of the yeast SUC2 gene. The SUC2 gene has been cloned (3) and its entire nucleotide sequence has been determined (34). SUC2 contains a single Bam HI site at codon 263 in its coding sequence. This site was eliminated by sodium bisulfite mutagenesis. 2 l'g of ...
suppression of the ras1 mutant phenotype. encoding a protein
... of the CDC25 gene (11), was unable to restore conjugal efficiency to SPR2A. To search for unknown suppressors of RASY"I-22, we screened plasmid libraries of S. pombe genomic DNA cloned into shuttle vectors for plasmids conferring conjugal efficiency to SPR2A upon transformation. Conjugation in Leu+ ...
... of the CDC25 gene (11), was unable to restore conjugal efficiency to SPR2A. To search for unknown suppressors of RASY"I-22, we screened plasmid libraries of S. pombe genomic DNA cloned into shuttle vectors for plasmids conferring conjugal efficiency to SPR2A upon transformation. Conjugation in Leu+ ...
3. Bacteria - la068.k12.sd.us
... Just like plants, bacteria have a cell wall to protect them from the elements. ...
... Just like plants, bacteria have a cell wall to protect them from the elements. ...
shRNA FAQ - Functional Genomics Facility
... TRC1.5 clones are in the exact same vector backbone as the TRC1 clones. Features of the pLKO.1-puro vector allow for transient or stable transfection of the shRNA as well as production of lentiviral particles. Stable gene silencing is selected using the puromycinselectable marker while self-inactiva ...
... TRC1.5 clones are in the exact same vector backbone as the TRC1 clones. Features of the pLKO.1-puro vector allow for transient or stable transfection of the shRNA as well as production of lentiviral particles. Stable gene silencing is selected using the puromycinselectable marker while self-inactiva ...
7.1 Techniques for Producing and Analyzing DNA
... Restriction endonuclease recognizes specific nucleotide sequences and cleaves the double stranded DNA. The enzyme will cut at the restriction site of the target sequence. Characteristics of Restriction Enzyme: a) Sequence Specificity: Each enzyme recognizes a specific sequence which it cut on every ...
... Restriction endonuclease recognizes specific nucleotide sequences and cleaves the double stranded DNA. The enzyme will cut at the restriction site of the target sequence. Characteristics of Restriction Enzyme: a) Sequence Specificity: Each enzyme recognizes a specific sequence which it cut on every ...
![2. Biotechnology Booklet [A2]](http://s1.studyres.com/store/data/000881801_1-016d6ea2a9343e8acf42bae0fafd1de9-300x300.png)
2. Biotechnology Booklet [A2]
... Livestock breeds frequently produce only one individual per pregnancy and all individuals in a herd will have different traits. Cloning (by embryo splitting or other means) makes it possible to produce high value herds with identical traits more quickly. This technique also has applications in the m ...
... Livestock breeds frequently produce only one individual per pregnancy and all individuals in a herd will have different traits. Cloning (by embryo splitting or other means) makes it possible to produce high value herds with identical traits more quickly. This technique also has applications in the m ...
No Origin, No Problem for Yeast DNA Replication
... To ensure proper transmission of genetic information, cells must accurately replicate their genome during each cell cycle. In budding yeast, DNA replication initiates from well-defined origins called autonomously replicating sequences (ARSs), while in multicellular organisms replication it is though ...
... To ensure proper transmission of genetic information, cells must accurately replicate their genome during each cell cycle. In budding yeast, DNA replication initiates from well-defined origins called autonomously replicating sequences (ARSs), while in multicellular organisms replication it is though ...
LABORATORY 3: Site-Directed Mutagenesis of Blue
... generation of random mutations in chromosomal DNA (such as those induced by X-rays and chemicals). Although these methods of random mutagenesis provided a valuable tool for classical genetic studies, the usefulness of the mutations was limited because it was not possible to target a specific gene or ...
... generation of random mutations in chromosomal DNA (such as those induced by X-rays and chemicals). Although these methods of random mutagenesis provided a valuable tool for classical genetic studies, the usefulness of the mutations was limited because it was not possible to target a specific gene or ...
PCR of GFP - the BIOTECH Project
... These are small volumes; you will need to be sure to look at the pipette tip when you are pipetting to make certain that the components are being added. Some groups will amplify control reaction, for each control reaction add the following to a PCR thin walled tube: ...
... These are small volumes; you will need to be sure to look at the pipette tip when you are pipetting to make certain that the components are being added. Some groups will amplify control reaction, for each control reaction add the following to a PCR thin walled tube: ...
19.1 Somatostatin Was the First Human Peptide Hormone Produced
... commercial potential. Instead, it was chosen because the researchers thought it would be technically less difficult than other hormones. Somatostatin is very small (only 14 amino acids long), which requires a short coding sequence, and it can be detected easily. Before discussing the details of this ...
... commercial potential. Instead, it was chosen because the researchers thought it would be technically less difficult than other hormones. Somatostatin is very small (only 14 amino acids long), which requires a short coding sequence, and it can be detected easily. Before discussing the details of this ...
Binding of ColEl-kan Plasmid DNA by Tobacco
... the screening procedure employed required that the kanamycin resistance gene be maintained within the plant cell for many generations. Maintenance via autonomous replication of the plasmid would require that the plasmid be taken up physically intact by the protoplasts. This may not have occurred. Th ...
... the screening procedure employed required that the kanamycin resistance gene be maintained within the plant cell for many generations. Maintenance via autonomous replication of the plasmid would require that the plasmid be taken up physically intact by the protoplasts. This may not have occurred. Th ...
5. Prokaryotes 11B
... 3. Parasitism = one organism is harmed while the other benefits (usually multi-cellular and nonlethal) Pathogen = similar to parasites, but more often unicellular and lethal ...
... 3. Parasitism = one organism is harmed while the other benefits (usually multi-cellular and nonlethal) Pathogen = similar to parasites, but more often unicellular and lethal ...
as a PDF
... Virulent phages = Phages that lyse their host cells. Lytic cycle = A viral replication cycle that results in the death or lysis of the host cell. The lytic cycle of phage T4 illustrates this type of replication cycle: 1. Phage attaches to cell surface. • T4 recognizes a host cell by a complementary ...
... Virulent phages = Phages that lyse their host cells. Lytic cycle = A viral replication cycle that results in the death or lysis of the host cell. The lytic cycle of phage T4 illustrates this type of replication cycle: 1. Phage attaches to cell surface. • T4 recognizes a host cell by a complementary ...
Characterization of the IEll0 Gene of Herpes Simplex Virus Type 1
... As shown in Fig. 1, IE gene 1 is located in the RL element of the HSV-1 genome. We have determined the complete sequence of RL, together with adjacent regions of UL: the whole sequence will be presented elsewhere (L. J. Perry & D. J. McGeoch, unpublished). Residue numbering in this paper is based on ...
... As shown in Fig. 1, IE gene 1 is located in the RL element of the HSV-1 genome. We have determined the complete sequence of RL, together with adjacent regions of UL: the whole sequence will be presented elsewhere (L. J. Perry & D. J. McGeoch, unpublished). Residue numbering in this paper is based on ...
Bacterial evolution and the cost of antibiotic resistance
... hosts with both the ancestral and evolved plasmids, giving four genotypes: B0/P0, B0/P500, B500/P0, and B500/P500, where B and P denote the bacteria and plasmid, respectively, and subscripts 0 and 500 indicate the ancestral (naive) and evolved forms, respectively. Each of these genotypes was placed ...
... hosts with both the ancestral and evolved plasmids, giving four genotypes: B0/P0, B0/P500, B500/P0, and B500/P500, where B and P denote the bacteria and plasmid, respectively, and subscripts 0 and 500 indicate the ancestral (naive) and evolved forms, respectively. Each of these genotypes was placed ...
Production of bacterial cellulose by agitation culture
... bacterial cellulose synthase activity have been bred by genetic engineering, with branches of their metabolic pathway blocked to decrease the amounts of by-products (refs.8,9). We bred mutant cells whose growth was increased to increase the enhancement of BC productivity (ref.10). Several host-vecto ...
... bacterial cellulose synthase activity have been bred by genetic engineering, with branches of their metabolic pathway blocked to decrease the amounts of by-products (refs.8,9). We bred mutant cells whose growth was increased to increase the enhancement of BC productivity (ref.10). Several host-vecto ...
Chapter 20
... Gel electrophoresis separates macromolecules on the basis of their rate of movement through a gel in an electric field. How far a DNA molecule travels while the current is on is inversely proportional to its length. A mixture of DNA molecules, usually fragments produced by restriction enzyme digesti ...
... Gel electrophoresis separates macromolecules on the basis of their rate of movement through a gel in an electric field. How far a DNA molecule travels while the current is on is inversely proportional to its length. A mixture of DNA molecules, usually fragments produced by restriction enzyme digesti ...
The Amino Terminus of the Yeast F1-ATPase B
... segment (3' of the structural gene) contained in this plasmid was removed by digestion with Xma 1 followed by SI nuclease treatment and ligation with T4 DNA ligase. This Sma I/Xma l--deleted derivative of YCp50 was then digested with Eco RI and Sal I and ligated with a 3.3-kb Eeo RI-Sal I DNA fragme ...
... segment (3' of the structural gene) contained in this plasmid was removed by digestion with Xma 1 followed by SI nuclease treatment and ligation with T4 DNA ligase. This Sma I/Xma l--deleted derivative of YCp50 was then digested with Eco RI and Sal I and ligated with a 3.3-kb Eeo RI-Sal I DNA fragme ...
Practice test 2
... DNA would be classified as a _____. a. clone c. plasmid b. DNA fingerprint d. transgenic organism 8. In 1974, Stanley Cohen and Herbert Boyer inserted a gene from an African clawed frog into a bacterium. The bacterium produced the protein coded for by the inserted frog gene. This insertion of a smal ...
... DNA would be classified as a _____. a. clone c. plasmid b. DNA fingerprint d. transgenic organism 8. In 1974, Stanley Cohen and Herbert Boyer inserted a gene from an African clawed frog into a bacterium. The bacterium produced the protein coded for by the inserted frog gene. This insertion of a smal ...
DNA technologies
... If cDNAs are made from the entire collection of mRNAs being expressed within a tissue or organism the collective cDNA clones when placed in a suitable plasmid are called a "cDNA library." This cDNA library should contain the entire repertoire of proteins being used by the host cells just before mRNA ...
... If cDNAs are made from the entire collection of mRNAs being expressed within a tissue or organism the collective cDNA clones when placed in a suitable plasmid are called a "cDNA library." This cDNA library should contain the entire repertoire of proteins being used by the host cells just before mRNA ...
Expression systems for industrial Gram
... of which have a limited host-range. vectors have been based on the which is functional in all studied ...
... of which have a limited host-range. vectors have been based on the which is functional in all studied ...
ScrFl restriction/modification system from
... 100 mM NaCl, 5 mM dithiothreitol and 2 mM 2-mercaptoethanol. Reactions were incubated at 37 "C for 60 min followed by electrophoresis. Biological ScrFI activity was assessed by comparing the titres of the lactococcal phage 4c2 on the host of interest relative to a non-restricting host. Enumeration o ...
... 100 mM NaCl, 5 mM dithiothreitol and 2 mM 2-mercaptoethanol. Reactions were incubated at 37 "C for 60 min followed by electrophoresis. Biological ScrFI activity was assessed by comparing the titres of the lactococcal phage 4c2 on the host of interest relative to a non-restricting host. Enumeration o ...
Plasmid
.svg?width=300)
A plasmid is a small DNA molecule within a cell that is physically separated from a chromosomal DNA and can replicate independently. They are most commonly found in bacteria as small, circular, double-stranded DNA molecules; however, plasmids are sometimes present in archaea and eukaryotic organisms. In nature, plasmids often carry genes that may benefit the survival of the organism, for example antibiotic resistance. While the chromosomes are big and contain all the essential information for living, plasmids usually are very small and contain only additional information. Artificial plasmids are widely used as vectors in molecular cloning, serving to drive the replication of recombinant DNA sequences within host organisms.Plasmids are considered replicons, a unit of DNA capable of replicating autonomously within a suitable host. However, plasmids, like viruses, are not generally classified as life. Plasmids can be transmitted from one bacterium to another (even of another species) via three main mechanisms: transformation, transduction, and conjugation. This host-to-host transfer of genetic material is called horizontal gene transfer, and plasmids can be considered part of the mobilome. Unlike viruses (which encase their genetic material in a protective protein coat called a capsid), plasmids are ""naked"" DNA and do not encode genes necessary to encase the genetic material for transfer to a new host. However, some classes of plasmids encode the conjugative ""sex"" pilus necessary for their own transfer. The size of the plasmid varies from 1 to over 200 kbp, and the number of identical plasmids in a single cell can range anywhere from one to thousands under some circumstances.The relationship between microbes and plasmid DNA is neither parasitic nor mutualistic, because each implies the presence of an independent species living in a detrimental or commensal state with the host organism. Rather, plasmids provide a mechanism for horizontal gene transfer within a population of microbes and typically provide a selective advantage under a given environmental state. Plasmids may carry genes that provide resistance to naturally occurring antibiotics in a competitive environmental niche, or the proteins produced may act as toxins under similar circumstances, or allow the organism to utilize particular organic compounds that would be advantageous when nutrients are scarce.