076075.Supplemental Data Text
... (IOD). In order to normalize for differences in total staining intensity between different 2-DE images, the amount of different spots were expressed as the percentage of the individual spot IOD per total IOD of all the spots (% IOD). The results were manually inspected and corrected when needed. We ...
... (IOD). In order to normalize for differences in total staining intensity between different 2-DE images, the amount of different spots were expressed as the percentage of the individual spot IOD per total IOD of all the spots (% IOD). The results were manually inspected and corrected when needed. We ...
Introduction to Metabolism
... Metabolic pathways consist of sequential steps. There are more than 2,000 metabolic reactions, each catalyzed by a distinct enzyme. The enzymes may be physically separate requiring the intermediate metabolites to diffuse from one active site to the next or enzymes may form a multienzyme complex whe ...
... Metabolic pathways consist of sequential steps. There are more than 2,000 metabolic reactions, each catalyzed by a distinct enzyme. The enzymes may be physically separate requiring the intermediate metabolites to diffuse from one active site to the next or enzymes may form a multienzyme complex whe ...
Title: Direct Analysis of Fexofenadine and Pseudoephedrine in
... subject. Urine samples were collected 2 and 4 hours postingestion for direct analysis by LAESI-MS. A 20 µL volume of each sample was deposited in the wells of a low volume 96-well plate and analyzed by LAESI-MS using the Protea LAESI DP-1000 system integrated with a Thermo ...
... subject. Urine samples were collected 2 and 4 hours postingestion for direct analysis by LAESI-MS. A 20 µL volume of each sample was deposited in the wells of a low volume 96-well plate and analyzed by LAESI-MS using the Protea LAESI DP-1000 system integrated with a Thermo ...
Chemical laboratories Dipl.-Ing.(FH) Giovanna
... Automated Determination of ammonium, phosphate, nitrate and nitrite by flow injection analysis (FIA) ...
... Automated Determination of ammonium, phosphate, nitrate and nitrite by flow injection analysis (FIA) ...
March 2017 - Human Metabolome Technologies
... arise from the need for cancer cells to generate the energy and biomass that is necessary to maintain cell growth and proliferation. As a result, cancer metabolism represents the greatest opportunity for the next generation of cancer therapeutics after the immunotherapy era. As our understanding evo ...
... arise from the need for cancer cells to generate the energy and biomass that is necessary to maintain cell growth and proliferation. As a result, cancer metabolism represents the greatest opportunity for the next generation of cancer therapeutics after the immunotherapy era. As our understanding evo ...
Characterisation of glycogenic and ketogenic metabolic pathways
... hydrolyzed casein on the postprandial diabetic glucose response. To study the effect of varying amino acid load (e.g. low glycine) in nutrients on growth processes in experimental tumor tissue and secondly the effect in patient cancers. Method: Based on the [1-13C]pyruvate (animals and humans) metab ...
... hydrolyzed casein on the postprandial diabetic glucose response. To study the effect of varying amino acid load (e.g. low glycine) in nutrients on growth processes in experimental tumor tissue and secondly the effect in patient cancers. Method: Based on the [1-13C]pyruvate (animals and humans) metab ...
PDF
... Hillenkamp, and K.Tanaka who developed the ion trap method, electrospray ionization, matrixassisted laser desorption ionization and soft laser desorption. Mass spectrometry is a powerful and sensitive technique which is used to detect, identify and quantitate molecules by measuring the mass-tocharge ...
... Hillenkamp, and K.Tanaka who developed the ion trap method, electrospray ionization, matrixassisted laser desorption ionization and soft laser desorption. Mass spectrometry is a powerful and sensitive technique which is used to detect, identify and quantitate molecules by measuring the mass-tocharge ...
A Study of the Effects of Exercise on the Urinary Metabolome
... downstream metabolic effects of a single type of exercise tailored specifically to each unique metabolome or even metabotype [8,9]. Exercise, as an external challenge to the human metabolome, creates an immediate response (turn-over rate in seconds [10]) across this biological matrix, which, unlike ...
... downstream metabolic effects of a single type of exercise tailored specifically to each unique metabolome or even metabotype [8,9]. Exercise, as an external challenge to the human metabolome, creates an immediate response (turn-over rate in seconds [10]) across this biological matrix, which, unlike ...
Chapter 14 Review Question Answers
... in the formation of the reactive o-quinones from these catechols). Thus, the two possible reactive metabolites are the arene-oxide and the o-quinone shown in Figure 3 of this paper. ...
... in the formation of the reactive o-quinones from these catechols). Thus, the two possible reactive metabolites are the arene-oxide and the o-quinone shown in Figure 3 of this paper. ...
Mass Spectrometry
... plate and coated with a matrix solution. The sample is then dried and introduced into a MALDI-TOF spectrometer which ionizes it and analyzes the ensuing mass spectra. If enough samples are taken a data array of the sampled tissue consisting of 1000-30,000 spots may be created to create a spatial map ...
... plate and coated with a matrix solution. The sample is then dried and introduced into a MALDI-TOF spectrometer which ionizes it and analyzes the ensuing mass spectra. If enough samples are taken a data array of the sampled tissue consisting of 1000-30,000 spots may be created to create a spatial map ...
Free Fatty acids - Sheffield Metabolic Laboratory
... as 3-hydroxybutyrate and free fatty acids (or non-esterified, NEFA). All are normally present in blood and have a vital role in energy metabolism. These compounds are linked through a number of different pathways, which interact depending on nutritional status. During normal nutritional status (i.e. ...
... as 3-hydroxybutyrate and free fatty acids (or non-esterified, NEFA). All are normally present in blood and have a vital role in energy metabolism. These compounds are linked through a number of different pathways, which interact depending on nutritional status. During normal nutritional status (i.e. ...
5713171075 Praveena EDUCATION Kingston University, London
... performed overall operational planning and activities for the implementation and conduct of studies (clinical trials, observational studies, compassionate use and registries) including data management activities in compliance with GCP’s, SOPs and standards within established timelines and budgets. P ...
... performed overall operational planning and activities for the implementation and conduct of studies (clinical trials, observational studies, compassionate use and registries) including data management activities in compliance with GCP’s, SOPs and standards within established timelines and budgets. P ...
Metabolic diseases of the liver
... Clinical patterns of metabolic disease involving the liver • Newborn acute metabolic crisis mimics sepsis • Severe vomiting and failure to thrive • Recurrent episodes of vomiting and encephalopathy with acidosis • Progressive retardation or seizures with hepatomegaly • Hepatomegaly with/without jau ...
... Clinical patterns of metabolic disease involving the liver • Newborn acute metabolic crisis mimics sepsis • Severe vomiting and failure to thrive • Recurrent episodes of vomiting and encephalopathy with acidosis • Progressive retardation or seizures with hepatomegaly • Hepatomegaly with/without jau ...
... INTRODUCTION The major bottleneck in metabolite identification is data processing because it is still mainly a manual process. The biggest challenge for automated data processing is the large number of false positives which may be generated. A powerful tool to help for the removal of these false pos ...
Eds., Y. Murakami, K. Nakayama, S.-I. Kitamura, H. Iwata and... © by TERRAPUB, 2008.
... characterize chemicals and environmental stresses (Iwahashi, 2000). In recent years, DNA microarray technology has developed rapidly and been widely adopted as a tool for understanding biological systems at the genomic level (Iwahashi, 2006). Furthermore, this technology can be combined with proteom ...
... characterize chemicals and environmental stresses (Iwahashi, 2000). In recent years, DNA microarray technology has developed rapidly and been widely adopted as a tool for understanding biological systems at the genomic level (Iwahashi, 2006). Furthermore, this technology can be combined with proteom ...
DOC - Uni Basel Research Database
... Organisation / Research unit Departement Biozentrum / Mass Spectrometry (Jenö) Project start 01.12.2015 Probable end 30.11.2016 Status Completed Exploring the proteome on a system-wide level is essential for obtaining information on the molecular mechanisms of diseases and fundamental biological pro ...
... Organisation / Research unit Departement Biozentrum / Mass Spectrometry (Jenö) Project start 01.12.2015 Probable end 30.11.2016 Status Completed Exploring the proteome on a system-wide level is essential for obtaining information on the molecular mechanisms of diseases and fundamental biological pro ...
Direct Drug Metabolism Monitoring in a Live Single Hepatic Cell by
... (cytoplasm, a vacuole or a nucleus) was sucked into a nanospray tip (HUMANIX) independently, as shown in Fig. 1. Then, 3 μL of a solvent (90% MeOH/8.0% H2O/2.0% formic acid) was added from the back-end of the tip. These samples were directly introduced by nano-electrospray ionization into a LTQ Orbi ...
... (cytoplasm, a vacuole or a nucleus) was sucked into a nanospray tip (HUMANIX) independently, as shown in Fig. 1. Then, 3 μL of a solvent (90% MeOH/8.0% H2O/2.0% formic acid) was added from the back-end of the tip. These samples were directly introduced by nano-electrospray ionization into a LTQ Orbi ...
P F I
... a core facility funded by the Vermont Genetics Network to serve the Lead Institution and research throughout the state as part of the IDeA Program mission of building research infrastructure. The facility provides a central resource of mass spectrometry-based proteomics technologies to identify, cha ...
... a core facility funded by the Vermont Genetics Network to serve the Lead Institution and research throughout the state as part of the IDeA Program mission of building research infrastructure. The facility provides a central resource of mass spectrometry-based proteomics technologies to identify, cha ...
Biochemistry of Metabolism Target Audience Those wishing to
... study of biochemistry and metabolism. Students will consider key concepts including: organic and inorganic chemistry, oxidation and reduction, acids and buffers, free radicals, biological molecules and enzymes. Content Students will then build up their understanding of metabolic pathways by learning ...
... study of biochemistry and metabolism. Students will consider key concepts including: organic and inorganic chemistry, oxidation and reduction, acids and buffers, free radicals, biological molecules and enzymes. Content Students will then build up their understanding of metabolic pathways by learning ...
Technological Innovations In Ayurveda Author name
... Mass Spectroscopy (MS)-Mass spectrometry is the most sensitive and selective method for molecular analysis and powder drug(inorganic) studies and can yield information on the molecular weight as well as the structure of the molecule. Combining chromatography with mass spectrometry provides the advan ...
... Mass Spectroscopy (MS)-Mass spectrometry is the most sensitive and selective method for molecular analysis and powder drug(inorganic) studies and can yield information on the molecular weight as well as the structure of the molecule. Combining chromatography with mass spectrometry provides the advan ...
Takeda/Duke/RTI meeting
... • Modules for sterols, phospholipids, and eicosanoids in development “Non-Targeted” MS Methods • ~1000 compound spectral library developed (with Agilent, Oliver Fiehn, UC Davis) for non-targeted GC/MS • LC-MS/MS for non-targeted analysis of thousands of metabolites/sample ...
... • Modules for sterols, phospholipids, and eicosanoids in development “Non-Targeted” MS Methods • ~1000 compound spectral library developed (with Agilent, Oliver Fiehn, UC Davis) for non-targeted GC/MS • LC-MS/MS for non-targeted analysis of thousands of metabolites/sample ...
Inference in Metabolic Network Models using Flux
... –! how the regulatory network interacts with the metabolic network •! in many cases, though, we can still make interesting predictions using constraint-based models •! key insight: instead of calculating exactly what a network does, narrow the range of possibilities by constraints ...
... –! how the regulatory network interacts with the metabolic network •! in many cases, though, we can still make interesting predictions using constraint-based models •! key insight: instead of calculating exactly what a network does, narrow the range of possibilities by constraints ...