![Supporting Information Text S4. Examples of de novo sequencing](http://s1.studyres.com/store/data/020646850_1-37a336a5d6875783c0266cc83235e600-300x300.png)
Supporting Information Text S4. Examples of de novo sequencing
... accurate measured mass of 1114.5144 Da (Fig. S4-1) a prominent row of y-type ions can be identified, most of them have also complementary b-type ions. The mass accuracy of all annotated peaks is less than 10 ppm (typically less than 2). The gap between 7th and 10th amino acids can be explained accor ...
... accurate measured mass of 1114.5144 Da (Fig. S4-1) a prominent row of y-type ions can be identified, most of them have also complementary b-type ions. The mass accuracy of all annotated peaks is less than 10 ppm (typically less than 2). The gap between 7th and 10th amino acids can be explained accor ...
Carbohydrate and sugar structure
... Chemolithotrophs – obtain free energy from oxidation of inorganic compounds such as NH3, H2S, or Fe2+. ...
... Chemolithotrophs – obtain free energy from oxidation of inorganic compounds such as NH3, H2S, or Fe2+. ...
Lecture Note 1
... isolation of novel antibiotics and antimicrobial peptides from various organisms. Individual components in the crude sample are isolated based on the differences in their physical and chemical properties. The bioactive component is identified by testing the activities of these isolated compounds. Th ...
... isolation of novel antibiotics and antimicrobial peptides from various organisms. Individual components in the crude sample are isolated based on the differences in their physical and chemical properties. The bioactive component is identified by testing the activities of these isolated compounds. Th ...
Appendix B HISS Codes for Metabolic Investigations
... The department also performs a very wide range of routine analyses and additionally supports sweat testing, endocrine, nutrition and gastroenterology teams with a comprehensive panel of tests. PRENATAL DIAGNOSES are undertaken for a wide variety of conditions. The diagnostic basis of the proband is ...
... The department also performs a very wide range of routine analyses and additionally supports sweat testing, endocrine, nutrition and gastroenterology teams with a comprehensive panel of tests. PRENATAL DIAGNOSES are undertaken for a wide variety of conditions. The diagnostic basis of the proband is ...
Lecture 19
... Chemolithotrophs – obtain free energy from oxidation of inorganic compounds such as NH3, H2S, or Fe2+. ...
... Chemolithotrophs – obtain free energy from oxidation of inorganic compounds such as NH3, H2S, or Fe2+. ...
ion exchange chromatography
... It is a non-denaturing technique. It can be used at all stages and scales of purification An IEX separation can be controlled by changing pH, salt concentration and/or the ion exchange media It can serve as a concentrating step. A large volume of dilute sample can be applied to a media, and the a ...
... It is a non-denaturing technique. It can be used at all stages and scales of purification An IEX separation can be controlled by changing pH, salt concentration and/or the ion exchange media It can serve as a concentrating step. A large volume of dilute sample can be applied to a media, and the a ...
Ultramer® Oligonucleotides - Integrated DNA Technologies
... of Ultramer Oligonucleotides for de novo gene construction. Many of the published procedures for constructing specific DNA sequences from oligos use relatively short sequences (≤60mer) with overlapping stretches of common sequence that permit annealing, followed by assembly using PCR-based strategie ...
... of Ultramer Oligonucleotides for de novo gene construction. Many of the published procedures for constructing specific DNA sequences from oligos use relatively short sequences (≤60mer) with overlapping stretches of common sequence that permit annealing, followed by assembly using PCR-based strategie ...
Kidney function test
... collection periods, for inulin assay. – GFR is taken as the mean for each period. ...
... collection periods, for inulin assay. – GFR is taken as the mean for each period. ...
Chem 301 Biological Chemistry I Laboratory Lab 7: Protein
... NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY You have learned about Nuclear magnetic resonance (NMR) with regard to organic molecule identification and chemical structure determination. But NMR is a powerful tool for studying biological molecules including proteins, nucleic acids, lipids and carbohydrate ...
... NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY You have learned about Nuclear magnetic resonance (NMR) with regard to organic molecule identification and chemical structure determination. But NMR is a powerful tool for studying biological molecules including proteins, nucleic acids, lipids and carbohydrate ...
Integration of Mammalian Metabolism
... •Interactions between tissues and organs are mediated by hormone signals carried via bloodstream. ...
... •Interactions between tissues and organs are mediated by hormone signals carried via bloodstream. ...
論文要旨・審査の要旨
... PCR-RFLP analyses. I have successfully completed and validated the SmartAmp primer set for accurate detection and discrimination of all possible homozygotes and heterozygotes of the SNP 2269G>A in the ABCC4 gene within 40 minutes. Multi-omics--The essential dynamic power behind comprehensive unders ...
... PCR-RFLP analyses. I have successfully completed and validated the SmartAmp primer set for accurate detection and discrimination of all possible homozygotes and heterozygotes of the SNP 2269G>A in the ABCC4 gene within 40 minutes. Multi-omics--The essential dynamic power behind comprehensive unders ...
Cardiac Energy Dependence on Glucose Increases
... 25°C/min to 290°C, and a final 8 minutes maintenance at 290°C. Electron impact ionization (70 eV) at full scan mode (m/z 40 to 600) was used, with an acquisition rate of 20 spectra/sec in the TOFMS setting. The data generated in the GC-TOFMS instrument were analyzed by the ChromaTOF software (v4.33; ...
... 25°C/min to 290°C, and a final 8 minutes maintenance at 290°C. Electron impact ionization (70 eV) at full scan mode (m/z 40 to 600) was used, with an acquisition rate of 20 spectra/sec in the TOFMS setting. The data generated in the GC-TOFMS instrument were analyzed by the ChromaTOF software (v4.33; ...
doc - EU-RL GMFF
... obtained for CRMs and subsequently on data from routine analysis. A minimum of 10 data to calculate the RSDr from an estimated 0.1% CRM-derived sample can be obtained in various ways but a valuable option could be to perform five extractions and two quantitative analyses on each extract. In this way ...
... obtained for CRMs and subsequently on data from routine analysis. A minimum of 10 data to calculate the RSDr from an estimated 0.1% CRM-derived sample can be obtained in various ways but a valuable option could be to perform five extractions and two quantitative analyses on each extract. In this way ...
THE APPLICATION OF TANDEM MASS SPECTROMETRY TO
... containing isotope-enriched internal standards is added. These standards are used to quantify Phe, Tyr, and the other α-AAs that are extracted into the methanol. It is important to note that quantification of the compounds in a DBS is different from clinical analyses that use liquid blood, plasma, o ...
... containing isotope-enriched internal standards is added. These standards are used to quantify Phe, Tyr, and the other α-AAs that are extracted into the methanol. It is important to note that quantification of the compounds in a DBS is different from clinical analyses that use liquid blood, plasma, o ...
CH 3
... How Build Novel Metabolic Pathways? • Whole metabolic pathways: no single gene or small number of genes confer selective advantage • Cannot build a step at a time Dilemma: how were entire pathways constructed during evolution? ...
... How Build Novel Metabolic Pathways? • Whole metabolic pathways: no single gene or small number of genes confer selective advantage • Cannot build a step at a time Dilemma: how were entire pathways constructed during evolution? ...
Role of fructose and uric acid in obesity and insulin resistance
... fructose-containing sugars. Fructose is distinct from other sugars in its initial metabolism via ketohexokinase (KHK), which bypasses regulated steps of glycolysis and rapidly depletes ATP followed by purine degradation via xanthine oxidoreductase (XOR) and, in addition, formation of the triglycerid ...
... fructose-containing sugars. Fructose is distinct from other sugars in its initial metabolism via ketohexokinase (KHK), which bypasses regulated steps of glycolysis and rapidly depletes ATP followed by purine degradation via xanthine oxidoreductase (XOR) and, in addition, formation of the triglycerid ...
A Rapid UPLC™ MS/MS Method for Determining Specific
... physiological amino acids using only a few microliters of blood within a period of approximately two (2) minutes. This technological advance has allowed for population-based screening of newborns for inherited disorders of amino acid metabolism and is now considered the standard of care in the Unite ...
... physiological amino acids using only a few microliters of blood within a period of approximately two (2) minutes. This technological advance has allowed for population-based screening of newborns for inherited disorders of amino acid metabolism and is now considered the standard of care in the Unite ...
Genome-Based Metabolic Mapping and C Flux
... modeling of dynamic isotopic labeling patterns (Young et al., 2011). This methodology allowed for mapping fluxes under autotrophic growth conditions (Young et al., 2011), because it overcame the limitation of traditional steady-state 13C metabolic flux analysis (MFA), which fails to resolve the flux fr ...
... modeling of dynamic isotopic labeling patterns (Young et al., 2011). This methodology allowed for mapping fluxes under autotrophic growth conditions (Young et al., 2011), because it overcame the limitation of traditional steady-state 13C metabolic flux analysis (MFA), which fails to resolve the flux fr ...
Identification of Aspartic and Isoaspartic Acid Residues in Amyloid β
... the deposition of Aβ peptides in sporadic AD.10 In vitro experiments showed increased fibrillogenesis and enhanced neurotoxicity of isoAsp23-containing Aβ peptides;10,19 however, other results suggested that Aβ aggregative ability and neurotoxicity were not enhanced by this modification.20 Similar s ...
... the deposition of Aβ peptides in sporadic AD.10 In vitro experiments showed increased fibrillogenesis and enhanced neurotoxicity of isoAsp23-containing Aβ peptides;10,19 however, other results suggested that Aβ aggregative ability and neurotoxicity were not enhanced by this modification.20 Similar s ...
HD Rx of Hyperammonemia (Gregory et al, Vol. 5,abst. 55P
... Conclusion Hyperammonemia is a medical emergency When medical management does not work consider RRT early HD should be used initially with HF in tandem Liver transplant should be considered if medical and RRT management is not ...
... Conclusion Hyperammonemia is a medical emergency When medical management does not work consider RRT early HD should be used initially with HF in tandem Liver transplant should be considered if medical and RRT management is not ...
Animal Energetics II PPT
... Animals control the inner workings of cells through the use of enzymes, which interconvert macromolecules to create building blocks and control the flow of energy ...
... Animals control the inner workings of cells through the use of enzymes, which interconvert macromolecules to create building blocks and control the flow of energy ...
New Mexico Medicaid Utilization Review Medical Justification for
... 1. Will the requested nutritional product be delivered by a feeding tube? If yes, please provide the diagnosis or the clinical condition that has led to the need for tube-feeding. ...
... 1. Will the requested nutritional product be delivered by a feeding tube? If yes, please provide the diagnosis or the clinical condition that has led to the need for tube-feeding. ...
Towards the storage metabolome: profiling the barley vacuole
... than protoplast levels (>90% of compound in the vacuole, >1.5 fold change (FC)), seven were invariant (30~90%, 0.5~1.5 FC) and seven displayed a lower vacuolar level (<30%, <0.5 FC) (Table I and Figure 1). Interestingly, histidine and alanine were high level more abundant in the barley vacuole than ...
... than protoplast levels (>90% of compound in the vacuole, >1.5 fold change (FC)), seven were invariant (30~90%, 0.5~1.5 FC) and seven displayed a lower vacuolar level (<30%, <0.5 FC) (Table I and Figure 1). Interestingly, histidine and alanine were high level more abundant in the barley vacuole than ...
Metabolite and isotopologue profiling in plants. Studies on the
... Table 1. The range of different strategies employed in metabolic experiments (Dunn et al., 2005) Metabolomics Non-biased identification and quantification of all metabolites in a biological system. The analytical techniques must be highly selective and sensitive. No one analytical technique, or comb ...
... Table 1. The range of different strategies employed in metabolic experiments (Dunn et al., 2005) Metabolomics Non-biased identification and quantification of all metabolites in a biological system. The analytical techniques must be highly selective and sensitive. No one analytical technique, or comb ...
Feasibility Study of using FAIMS to Detect Carbonyl Sulfide in Propane
... Sample preparation and introduction FAIMS can be used to detect volatiles in aqueous, solid and gaseous matrices and can consequently be used for a wide variety of applications. The user requirements and sample matrix for each application define the sample preparation and introduction steps required ...
... Sample preparation and introduction FAIMS can be used to detect volatiles in aqueous, solid and gaseous matrices and can consequently be used for a wide variety of applications. The user requirements and sample matrix for each application define the sample preparation and introduction steps required ...