Isolation of Spherosomes with Lysosome Characteristics from
... Fractionation of cell free extracts from corn and tobacco seedlings by density gradient centri fugation resulted in the isolation of two fractions containing acid hydrolases: protease, phos phatase, esterase and ribonuclease. In one fraction prospherosomes were the predominant cyto plasmic struct ...
... Fractionation of cell free extracts from corn and tobacco seedlings by density gradient centri fugation resulted in the isolation of two fractions containing acid hydrolases: protease, phos phatase, esterase and ribonuclease. In one fraction prospherosomes were the predominant cyto plasmic struct ...
Identification and localization of a β‐COP‐like protein involved in the
... b-COP proteins are part of a complex named the coatomer, which probably recruits some speci®c areas of the endomembrane system. Immuno¯uorescence analyses by confocal laser scanning microscopy showed that b-COP-like proteins marked predominantly the plant Golgi apparatus. Other proteins known to be ...
... b-COP proteins are part of a complex named the coatomer, which probably recruits some speci®c areas of the endomembrane system. Immuno¯uorescence analyses by confocal laser scanning microscopy showed that b-COP-like proteins marked predominantly the plant Golgi apparatus. Other proteins known to be ...
Isolation of Spherosomes with Lysosome
... Fractionation of cell free extracts from corn and tobacco seedlings by density gradient centri fugation resulted in the isolation of two fractions containing acid hydrolases: protease, phos phatase, esterase and ribonuclease. In one fraction prospherosomes were the predominant cyto plasmic struct ...
... Fractionation of cell free extracts from corn and tobacco seedlings by density gradient centri fugation resulted in the isolation of two fractions containing acid hydrolases: protease, phos phatase, esterase and ribonuclease. In one fraction prospherosomes were the predominant cyto plasmic struct ...
Bronxville HS Page 1 of 8 AP Biology Mr. Ippolito AP Biology
... Describe the structure and function of the nucleus and its component parts as found in eukaryotic cells. ...
... Describe the structure and function of the nucleus and its component parts as found in eukaryotic cells. ...
MyoD as a gatekeeper to cell cycle progression
... absence of MyoD phosphorylation. In wildtype cells, p21 binds cyclin B-Cdc2 in G2 repressing the complex’s activity. p21 levels decline rapidly upon entry into mitosis, releasing cyclin B-Cdc2 from its inhibitory effects. An uncontrolled buildup of p21 would, therefore, prevent cyclin B-Cdc2 from in ...
... absence of MyoD phosphorylation. In wildtype cells, p21 binds cyclin B-Cdc2 in G2 repressing the complex’s activity. p21 levels decline rapidly upon entry into mitosis, releasing cyclin B-Cdc2 from its inhibitory effects. An uncontrolled buildup of p21 would, therefore, prevent cyclin B-Cdc2 from in ...
Live imaging genetically-encoded fluorescent proteins in embryonic
... The pluripotency of ES cells, their ease of manipulation in culture, and their ability to contribute to the mouse germline, has not only facilitated a plethora of strategies for genetic modifications at base pair resolution (See for examples [55-57]), but has also provided a model of differentiation ...
... The pluripotency of ES cells, their ease of manipulation in culture, and their ability to contribute to the mouse germline, has not only facilitated a plethora of strategies for genetic modifications at base pair resolution (See for examples [55-57]), but has also provided a model of differentiation ...
SNAREs: Cogs and Coordinators in Signaling
... bundle of coiled helices that draws the membrane surfaces together for docking and fusion (Fig. 1). Elements of this SNARE complex differ widely in size and structure, but they share common structural motifs, notably those contributing to interactions at the core of the SNARE complex. Within the bun ...
... bundle of coiled helices that draws the membrane surfaces together for docking and fusion (Fig. 1). Elements of this SNARE complex differ widely in size and structure, but they share common structural motifs, notably those contributing to interactions at the core of the SNARE complex. Within the bun ...
Phenotypic Analysis of Temperature-Sensitive Yeast Actin Mutants.
... 2A). Often there is a concentration of patches at the neck of the bud. Permissively grown actl-2 cells exhibit a different phenotype. Bars or ribbons of actin appear to cross the mother cell diametrically; the bars do not pass through the nucleus, nor do they show an orientation toward the bud (Figu ...
... 2A). Often there is a concentration of patches at the neck of the bud. Permissively grown actl-2 cells exhibit a different phenotype. Bars or ribbons of actin appear to cross the mother cell diametrically; the bars do not pass through the nucleus, nor do they show an orientation toward the bud (Figu ...
Gene transcription is coordinated with, but not dependent on, cell
... worms on the plates were L4, and embryos were harvested the next day. MQ130 were harvested after growth in asynchronous culture for several generations at constant temperature (20°C). To obtain fixed embryos, worms ...
... worms on the plates were L4, and embryos were harvested the next day. MQ130 were harvested after growth in asynchronous culture for several generations at constant temperature (20°C). To obtain fixed embryos, worms ...
A GTPase gate for protein import into chloroplasts
... The scenario of a preprotein targeting cycle involving cognate GTPases bears an uncanny resemblance to the targeting of the ribosome–nascent chain complexes (RNC) to the endoplasmic reticulum (ER) membrane by the signal recognition particle (SRP) and SRP receptor (SR)20. SRP associates with RNCs in ...
... The scenario of a preprotein targeting cycle involving cognate GTPases bears an uncanny resemblance to the targeting of the ribosome–nascent chain complexes (RNC) to the endoplasmic reticulum (ER) membrane by the signal recognition particle (SRP) and SRP receptor (SR)20. SRP associates with RNCs in ...
Plant nuclear bodies
... into the nucleus, where they form protein complexes and accumulate in speckle-like nuclear domains [50,51,52]. The biological function of these domains is unknown but their formation is related to function and light response. For example, the domains vary in size and content of the active phytoch ...
... into the nucleus, where they form protein complexes and accumulate in speckle-like nuclear domains [50,51,52]. The biological function of these domains is unknown but their formation is related to function and light response. For example, the domains vary in size and content of the active phytoch ...
Reading Science! 6.12CD: Classification of Organisms What’s In A Name? Lexile 870L
... meant to say that it doesn’t matter what we call something. It may not matter to fiction writers what things are called, but it is very important to scientists. Scientists need a way to organize and classify groups of life forms. How scientists do that has changed over time, as we have learned more ...
... meant to say that it doesn’t matter what we call something. It may not matter to fiction writers what things are called, but it is very important to scientists. Scientists need a way to organize and classify groups of life forms. How scientists do that has changed over time, as we have learned more ...
Optical tweezers - Niels Bohr Institutet
... To address possible physiological damage by a 1064 nm laser on living organisms, different bifferent bacterial types were optically trapped. • Simultaneously, the emission from a pH sensitive fluorophore (CFDA, GFP) inside the organism was monitored. • The capability of a cell to maintain a pH gradi ...
... To address possible physiological damage by a 1064 nm laser on living organisms, different bifferent bacterial types were optically trapped. • Simultaneously, the emission from a pH sensitive fluorophore (CFDA, GFP) inside the organism was monitored. • The capability of a cell to maintain a pH gradi ...
Non-Flagellar Swimming in Marine Synechococcus
... manipulation of Synechococcus has made it possible to address the problem at the molecular level (Brahamsha, 1996b). In the course of experiments designed to detect cell-surface components that may be involved in swimming, a major polypeptide of 130 kDa was identified whose decrease in abundance fol ...
... manipulation of Synechococcus has made it possible to address the problem at the molecular level (Brahamsha, 1996b). In the course of experiments designed to detect cell-surface components that may be involved in swimming, a major polypeptide of 130 kDa was identified whose decrease in abundance fol ...
Full Text - The International Journal of Developmental Biology
... segments. These 8nuclear threads", named chromosomes a decade later, then would split lengthwise. The halves would move towards the poles of the "achromatic" spindle, to transform there into the chromatin of newly forming daughter nuclei and cells. Flemming being a pathologist, the main aim he procl ...
... segments. These 8nuclear threads", named chromosomes a decade later, then would split lengthwise. The halves would move towards the poles of the "achromatic" spindle, to transform there into the chromatin of newly forming daughter nuclei and cells. Flemming being a pathologist, the main aim he procl ...
Topological characterization of the essential Escherichia coli cell
... ftsW gene. To generate L-lactamase fusions we followed the method described by [17]. Various truncated forms of the ftsW gene (at the 3P end) were ampli¢ed by PCR from the E. coli chromosomal DNA using as primers oligonucleotides generating a KpnI site. The oligonucleotide A60 (5P-CGTACCAGGTACCGCCTG ...
... ftsW gene. To generate L-lactamase fusions we followed the method described by [17]. Various truncated forms of the ftsW gene (at the 3P end) were ampli¢ed by PCR from the E. coli chromosomal DNA using as primers oligonucleotides generating a KpnI site. The oligonucleotide A60 (5P-CGTACCAGGTACCGCCTG ...
Chapter 3 Cells and Tissues
... equal to the outside. • Hypertonic- the concentration of solutes is greater outside the cell than inside it. ...
... equal to the outside. • Hypertonic- the concentration of solutes is greater outside the cell than inside it. ...
Novel functions for Rab GTPases in multiple aspects of tumour
... Rab proteins are part of the large Ras superfamily of small GTPases. There are more than 60 members in humans and each of them is specifically localized to a subcellular membrane compartment, of which it controls the intracellular traffic [1]. Rab proteins cycle between an inactive cytosolic GDP-bou ...
... Rab proteins are part of the large Ras superfamily of small GTPases. There are more than 60 members in humans and each of them is specifically localized to a subcellular membrane compartment, of which it controls the intracellular traffic [1]. Rab proteins cycle between an inactive cytosolic GDP-bou ...
Catalog 2 Version: January 2013 University of Illinois at Chicago
... of IG20 gene expression renders thyroid cancer cells susceptible to apoptosis. J Clin Endocrinol Metab. 2009 Apr;94(4):1467-71. ...
... of IG20 gene expression renders thyroid cancer cells susceptible to apoptosis. J Clin Endocrinol Metab. 2009 Apr;94(4):1467-71. ...
A fibroblast is a type of cell that synthesizes the extracellular matrix
... monolayers and are not restricted by a polarizing attachment to a basal lamina on one side, although they may contribute to basal lamina components in some situations (eg subepithelial myofibroblasts in intestine may secrete the α-2 chain carrying component of the laminin which is absent only in reg ...
... monolayers and are not restricted by a polarizing attachment to a basal lamina on one side, although they may contribute to basal lamina components in some situations (eg subepithelial myofibroblasts in intestine may secrete the α-2 chain carrying component of the laminin which is absent only in reg ...
Isolation of Spherosomes with Lysosome Characteristics from
... Fractionation of cell free extracts from corn and tobacco seedlings by density gradient centri fugation resulted in the isolation of two fractions containing acid hydrolases: protease, phos phatase, esterase and ribonuclease. In one fraction prospherosomes were the predominant cyto plasmic struct ...
... Fractionation of cell free extracts from corn and tobacco seedlings by density gradient centri fugation resulted in the isolation of two fractions containing acid hydrolases: protease, phos phatase, esterase and ribonuclease. In one fraction prospherosomes were the predominant cyto plasmic struct ...
Dynamical Neuroscience: A Viewpoint
... • “barriers to collaborations between mathematicians and biologists” • “a shift from mathematical analysis to computer simulation due mostly to improvements in computer power and accessibility.. With the shift being made possible to include more information in models and still derive useful insights ...
... • “barriers to collaborations between mathematicians and biologists” • “a shift from mathematical analysis to computer simulation due mostly to improvements in computer power and accessibility.. With the shift being made possible to include more information in models and still derive useful insights ...
Chemotaxis
... By integrating over time it solves both problems Berg (1988) • if memory is 1 min and bacterium is moving at 30 m sec-1, concentration comparison is being made over ~2 mm, or 1000-bodylengths – lessens accuracy needed to 1 in 10 • memory is actually only ~1-10 sec • Why not have longer memory? - inf ...
... By integrating over time it solves both problems Berg (1988) • if memory is 1 min and bacterium is moving at 30 m sec-1, concentration comparison is being made over ~2 mm, or 1000-bodylengths – lessens accuracy needed to 1 in 10 • memory is actually only ~1-10 sec • Why not have longer memory? - inf ...
The song of lipids and proteins: dynamic lipid
... in seven PPI species. PPIs have been implicated in a plethora of processes and their classic ‘textbook’ role is to serve as substrates for the generation of secondary messengers (typically diacylglycerol (DAG) and inositol 1,4,5-trisphosphate ...
... in seven PPI species. PPIs have been implicated in a plethora of processes and their classic ‘textbook’ role is to serve as substrates for the generation of secondary messengers (typically diacylglycerol (DAG) and inositol 1,4,5-trisphosphate ...
Cytokinesis
Cytokinesis (cyto- + kinesis) is the process during cell division in which the cytoplasm of a single eukaryotic cell is divided to form two daughter cells. It usually initiates during the early stages of mitosis, and sometimes meiosis, splitting a mitotic cell in two, to ensure that chromosome number is maintained from one generation to the next. After cytokinesis two (daughter) cells will be formed that are exact copies of the (parent) original cell. After cytokinesis, each daughter cell is in the interphase portion of the cell cycle. In animal cells, one notable exception to the normal process of cytokinesis is oogenesis (the creation of an ovum in the ovarian follicle of the ovary), where the ovum takes almost all the cytoplasm and organelles, leaving very little for the resulting polar bodies, which then die. Another form of mitosis without cytokinesis occurs in the liver, yielding multinucleate cells. In plant cells, a dividing structure known as the cell plate forms within the centre of the cytoplasm and a new cell wall forms between the two daughter cells.Cytokinesis is distinguished from the prokaryotic process of binary fission.