Efficient delivery of active Cas9 protein and targetspecific sgRNA to
... Heterodimerizer ligand. Gesicles were collected from the media 48–72 hours later, concentrated via centrifugation, and stored at −70°C until use on target cells. Knockout of ZsGreen1 in a broad range of cell types HEK 293T, HeLa, MCF7, NIH3T3, HT1080, CHOK1, RPE, Raji, Jurkat, HepG2, and KBM7 cell ...
... Heterodimerizer ligand. Gesicles were collected from the media 48–72 hours later, concentrated via centrifugation, and stored at −70°C until use on target cells. Knockout of ZsGreen1 in a broad range of cell types HEK 293T, HeLa, MCF7, NIH3T3, HT1080, CHOK1, RPE, Raji, Jurkat, HepG2, and KBM7 cell ...
Supplementary Methods, Figure, Table and Movie Legends
... frame activation pulse. Images were captured every 5 s for 75 frames. At least three animals were imaged for each probe, and care was taken to minimize pixel saturation in the raw data. The level of photo-bleaching which did not cause photo-damage was estimated to be 40 % in vivo, and therefore limi ...
... frame activation pulse. Images were captured every 5 s for 75 frames. At least three animals were imaged for each probe, and care was taken to minimize pixel saturation in the raw data. The level of photo-bleaching which did not cause photo-damage was estimated to be 40 % in vivo, and therefore limi ...
Two yeast forkhead genes regulate the cell cycle and pseudohyphal growth.
... expression is seen when the SFF sites are removed from the CLB2 promoter5,6. The constitutive expression of the genes in the CLB2 cluster explains why the fkh1 fkh2 mutant is viable. Misregulation of genes in the CLB2 cluster might result in secondary effects. In particular, the CLB2 cluster encodes ...
... expression is seen when the SFF sites are removed from the CLB2 promoter5,6. The constitutive expression of the genes in the CLB2 cluster explains why the fkh1 fkh2 mutant is viable. Misregulation of genes in the CLB2 cluster might result in secondary effects. In particular, the CLB2 cluster encodes ...
THE ORGANISMS
... A plant such as the one in the drawing above looks and behaves very differently from insects that might feed on it and from fungi that might grow on its roots. Likewise, different types of single-celled organisms, such as amebas and bacteria, vary in appearance. Differences in the cells of the organ ...
... A plant such as the one in the drawing above looks and behaves very differently from insects that might feed on it and from fungi that might grow on its roots. Likewise, different types of single-celled organisms, such as amebas and bacteria, vary in appearance. Differences in the cells of the organ ...
APOPTOSIS: An overview
... Decrease in ATP due to failure of mitochondrial energy production by oxidants ...
... Decrease in ATP due to failure of mitochondrial energy production by oxidants ...
DOT1A-dependent H3K76 methylation is required for replication
... We reported previously that H3K76me2 is mainly restricted to mitosis and cytokinesis (2). To obtain a complete picture of the H3K76 methylation pattern during the cell cycle, we generated an antibody specific for H3K76 mono-methylation. The specificity of the anti-H3K76me1 antibody was confirmed by pep ...
... We reported previously that H3K76me2 is mainly restricted to mitosis and cytokinesis (2). To obtain a complete picture of the H3K76 methylation pattern during the cell cycle, we generated an antibody specific for H3K76 mono-methylation. The specificity of the anti-H3K76me1 antibody was confirmed by pep ...
Cell Project
... c. There must be some type of background music – it can be a real song or you can make up your own beat, either way, please do not turn in a video of you talking the lyrics. You will NOT receive credit if that is done! d. The lyrics of the song/rap must be understandable (if you would like to edit y ...
... c. There must be some type of background music – it can be a real song or you can make up your own beat, either way, please do not turn in a video of you talking the lyrics. You will NOT receive credit if that is done! d. The lyrics of the song/rap must be understandable (if you would like to edit y ...
View PDF
... of Stem Cell Research and Therapy you will find reviews that discuss different aspects of adult muscle stem cell biology. Work over the last 50 years attributed to satellite cells, which are muscle stem cells closely associated with the myofibers, a central role in mediating the regenerative respons ...
... of Stem Cell Research and Therapy you will find reviews that discuss different aspects of adult muscle stem cell biology. Work over the last 50 years attributed to satellite cells, which are muscle stem cells closely associated with the myofibers, a central role in mediating the regenerative respons ...
Chapter 13
... • Endospores – Unique structures produced by some bacteria that are a defensive strategy against unfavorable conditions ...
... • Endospores – Unique structures produced by some bacteria that are a defensive strategy against unfavorable conditions ...
It is essential for students to know the three major tenets of the cell
... In the development of most multicellular organisms, a single cell (fertilized egg) gives rise to many different types of cells, each with a different structure and corresponding function. ○ The fertilized egg gives rise to a large number of cells through cell division, but the process of cell divi ...
... In the development of most multicellular organisms, a single cell (fertilized egg) gives rise to many different types of cells, each with a different structure and corresponding function. ○ The fertilized egg gives rise to a large number of cells through cell division, but the process of cell divi ...
Final review packet
... 1. Complete the final review study guide on the next page (must receive at least 75%) (35 points) 2. Have the contract at the bottom of this page signed 1. Final review study guide: On the following page is a list of prompts similar to your study guides throughout the semester and organized to refle ...
... 1. Complete the final review study guide on the next page (must receive at least 75%) (35 points) 2. Have the contract at the bottom of this page signed 1. Final review study guide: On the following page is a list of prompts similar to your study guides throughout the semester and organized to refle ...
classkingdomsppt
... tell the difference between living, dead, and non-living things. Prior to the 1600's many people ...
... tell the difference between living, dead, and non-living things. Prior to the 1600's many people ...
The Myriad Roles of Anillin during Cytokinesis Alisa J. Piekny1 and
... In further support for the idea that Anillin regulates or stabilizes RhoA localization, Anillin also interacts with Ect2 (Table 1), an activator of RhoA (A. J. Piekny, unpublished observations). This interaction occurs independently of RhoA and requires the AH domain of Anillin and the PH domain of ...
... In further support for the idea that Anillin regulates or stabilizes RhoA localization, Anillin also interacts with Ect2 (Table 1), an activator of RhoA (A. J. Piekny, unpublished observations). This interaction occurs independently of RhoA and requires the AH domain of Anillin and the PH domain of ...
Wellcome Trust Centre For Cell
... As a result of surgery or injury, our internal organs may become damaged, with associated internal bleeding. As the organ heals, cells move into the blood clot which over time is replaced by unwanted and painful fibrous tissue known as an ‘adhesion’. We have studied the surface of tendons and discov ...
... As a result of surgery or injury, our internal organs may become damaged, with associated internal bleeding. As the organ heals, cells move into the blood clot which over time is replaced by unwanted and painful fibrous tissue known as an ‘adhesion’. We have studied the surface of tendons and discov ...
E - U of L Class Index
... reverse reaction. This makes the reaction reversible – which is necessary in order to accurately measure the cell potential. To completely describe the electrochemical cell, we list the reactants and products at both electrodes – with the oxidation reaction at the left so that the electrons “flow” f ...
... reverse reaction. This makes the reaction reversible – which is necessary in order to accurately measure the cell potential. To completely describe the electrochemical cell, we list the reactants and products at both electrodes – with the oxidation reaction at the left so that the electrons “flow” f ...
Standard B-2
... • In the development of most multicellular organisms, a single cell (fertilized egg) gives rise to many different types of cells, each with a different structure and corresponding function. ○ The fertilized egg gives rise to a large number of cells through cell division, but the process of cell divi ...
... • In the development of most multicellular organisms, a single cell (fertilized egg) gives rise to many different types of cells, each with a different structure and corresponding function. ○ The fertilized egg gives rise to a large number of cells through cell division, but the process of cell divi ...
Cells: The Building Blocks of Life
... small society. It must receive food and water in order to survive. This food must be converted into energy and other valuable nutrients. The nutrients have to be processed, packaged and sent to the right part of the cell. Certain balances must be maintained inside the cell in order for it to survive ...
... small society. It must receive food and water in order to survive. This food must be converted into energy and other valuable nutrients. The nutrients have to be processed, packaged and sent to the right part of the cell. Certain balances must be maintained inside the cell in order for it to survive ...
Competency Goal 2 - Pitt County Schools
... How does compartmentalization organize a cell’s functions? How are the structures of the various subcellular organelles related to their functions? How do organelles function together in cellular processes? What factors limit cell size? MEMBRANES (Chapter 8) What is the current model of the molecula ...
... How does compartmentalization organize a cell’s functions? How are the structures of the various subcellular organelles related to their functions? How do organelles function together in cellular processes? What factors limit cell size? MEMBRANES (Chapter 8) What is the current model of the molecula ...
Biology 11 - Correspondence Studies
... trees and flowers are living; but what about things, such as bacteria and viruses, that cannot be seen with the naked eye? With the invention of the microscope, the microscopic world was discovered. The distinction between living and non-living became more difficult to discern. Through years of resear ...
... trees and flowers are living; but what about things, such as bacteria and viruses, that cannot be seen with the naked eye? With the invention of the microscope, the microscopic world was discovered. The distinction between living and non-living became more difficult to discern. Through years of resear ...
1 - ISpatula
... which are decrease of brain size or heart size to meet the diminished blood supply. 4-Inadiquant nutrition: malnutrition especially protein malnutrition, the body tends to use muscle as a source of energy because of depleted all other stores, which called (muscle wasting) or chchexia, this mainly se ...
... which are decrease of brain size or heart size to meet the diminished blood supply. 4-Inadiquant nutrition: malnutrition especially protein malnutrition, the body tends to use muscle as a source of energy because of depleted all other stores, which called (muscle wasting) or chchexia, this mainly se ...
Potassium balance
... Excretion = intake Normally urinary excretion = intake – (small amount ...
... Excretion = intake Normally urinary excretion = intake – (small amount ...
Diffusion Worksheet
... Define simple diffusion: ____________________________________________________________ Define dialysis: __________________________________________________________________ Define osmosis: __________________________________________________________________ Define facilitated diffusion: _________________ ...
... Define simple diffusion: ____________________________________________________________ Define dialysis: __________________________________________________________________ Define osmosis: __________________________________________________________________ Define facilitated diffusion: _________________ ...
Mitosis
Mitosis is a part of the cell cycle in which chromosomes in a cell nucleus are separated into two identical sets of chromosomes, each in its own nucleus. In general, mitosis (division of the nucleus) is often followed by cytokinesis, which divides the cytoplasm, organelles and cell membrane into two new cells containing roughly equal shares of these cellular components. Mitosis and cytokinesis together define the mitotic (M) phase of an animal cell cycle—the division of the mother cell into two daughter cells, genetically identical to each other and to their parent cell.The process of mitosis is divided into stages corresponding to the completion of one set of activities and the start of the next. These stages are prophase, prometaphase, metaphase, anaphase, and telophase. During mitosis, the chromosomes, which have already duplicated, condense and attach to fibers that pull one copy of each chromosome to opposite sides of the cell. The result is two genetically identical daughter nuclei. The cell may then divide by cytokinesis to produce two daughter cells. Producing three or more daughter cells instead of normal two is a mitotic error called tripolar mitosis or multipolar mitosis (direct cell triplication / multiplication). Other errors during mitosis can induce apoptosis (programmed cell death) or cause mutations. Certain types of cancer can arise from such mutations.Mitosis occurs only in eukaryotic cells and the process varies in different organisms. For example, animals undergo an ""open"" mitosis, where the nuclear envelope breaks down before the chromosomes separate, while fungi undergo a ""closed"" mitosis, where chromosomes divide within an intact cell nucleus. Furthermore, most animal cells undergo a shape change, known as mitotic cell rounding, to adopt a near spherical morphology at the start of mitosis. Prokaryotic cells, which lack a nucleus, divide by a different process called binary fission.