press release
... Two years after starting his post-doc at the MRC-LMB, Jan fast-tracked to become a Group Leader, and has focused on "Molecular Machines." Recently, his group has discovered another protein in bacteria that was thought to be a 'signature' protein for eukaryotic cells: dynamin. They continue to explor ...
... Two years after starting his post-doc at the MRC-LMB, Jan fast-tracked to become a Group Leader, and has focused on "Molecular Machines." Recently, his group has discovered another protein in bacteria that was thought to be a 'signature' protein for eukaryotic cells: dynamin. They continue to explor ...
Lamin proteins form an internal nucleoskeleton as well as a
... Encapsulated and eluted cells were lightly fixed (10 minutes; 0°C) with 0.1% glutaraldehyde or 1% paraformaldehyde in PB, washed 2× in 0.1 M Na/K phosphate Sörensen buffer (SB; pH 7.4), incubated (10 minutes) in 0.02 M glycine in SB, and rewashed in SB. After incubation (20 minutes; 20°C) with 5% no ...
... Encapsulated and eluted cells were lightly fixed (10 minutes; 0°C) with 0.1% glutaraldehyde or 1% paraformaldehyde in PB, washed 2× in 0.1 M Na/K phosphate Sörensen buffer (SB; pH 7.4), incubated (10 minutes) in 0.02 M glycine in SB, and rewashed in SB. After incubation (20 minutes; 20°C) with 5% no ...
Induction of fungal cell wall stress
... suspension was diluted to the concentration of 2 x 104 CFU/mL in the complete media (CM) [Bennett and Lasure, 1991]. One hundred μL of A. niger (RD 6.47 or JD 1.1) was added to each well after adding the plant extracts, compounds and controls. Aspergillus niger N402 was used to indicate the GFP non- ...
... suspension was diluted to the concentration of 2 x 104 CFU/mL in the complete media (CM) [Bennett and Lasure, 1991]. One hundred μL of A. niger (RD 6.47 or JD 1.1) was added to each well after adding the plant extracts, compounds and controls. Aspergillus niger N402 was used to indicate the GFP non- ...
Full Text - Journal of Skin and Stem Cell
... Results: Stem cells located in skin cells have shown interesting properties such as plasticity, transdifferentiation, and specificity. Mesenchymal cells of the dermis, hypodermis, and other sources are currently being investigated to promote regeneration. Conclusions: Because skin stem cells are hig ...
... Results: Stem cells located in skin cells have shown interesting properties such as plasticity, transdifferentiation, and specificity. Mesenchymal cells of the dermis, hypodermis, and other sources are currently being investigated to promote regeneration. Conclusions: Because skin stem cells are hig ...
08 Cytokinins
... Specific role -- regulate the progression of the cell cycle. In absence of either auxin or cytokinin -- G1 or G2 Supply missing hormone -- 12-24 hours – division begins Activate cyclin-dependent kinases (CDK). Activation of CDK – allows transition from G2 to mitosis. Promotion of accumulatio ...
... Specific role -- regulate the progression of the cell cycle. In absence of either auxin or cytokinin -- G1 or G2 Supply missing hormone -- 12-24 hours – division begins Activate cyclin-dependent kinases (CDK). Activation of CDK – allows transition from G2 to mitosis. Promotion of accumulatio ...
Targeted delivery of a novel anticancer compound anisomelic acid using chitosan-
... search for agents that may treat or ameliorate the affiliation of cancer, natural products have provided an endless supply of active compounds that are increasingly being exploited. In many cases, this quest has already resulted in leads and scaffolds for drugs1-3 and many reports highlight the use ...
... search for agents that may treat or ameliorate the affiliation of cancer, natural products have provided an endless supply of active compounds that are increasingly being exploited. In many cases, this quest has already resulted in leads and scaffolds for drugs1-3 and many reports highlight the use ...
Carbohydrates Learning Objective Carbohydrates
... Glycosyl transferases are specific enzymes that bring about transfer of activated sugar residues onto other substrates. The activated sugar linked to a nucleotide moiety such as UDP is cleaved and covalently linked with the substrate which could either be another monosaccharide unit, a polysaccharid ...
... Glycosyl transferases are specific enzymes that bring about transfer of activated sugar residues onto other substrates. The activated sugar linked to a nucleotide moiety such as UDP is cleaved and covalently linked with the substrate which could either be another monosaccharide unit, a polysaccharid ...
Lymphocyte macrophage interactions: peripolesis of A. D.J.
... in size, enzyme content, motility and membrane markers [4]. Some lavage fluids contain veiled or dendritic antigen-presenting cells (APCs), which resemble macrophages but are not phagocytic or adherent and are also highly mobile [5]. The two types of APC have different functions. The macrophages ing ...
... in size, enzyme content, motility and membrane markers [4]. Some lavage fluids contain veiled or dendritic antigen-presenting cells (APCs), which resemble macrophages but are not phagocytic or adherent and are also highly mobile [5]. The two types of APC have different functions. The macrophages ing ...
The detection of nuclear matrix in most primitive present
... nucleus may express some characteristics of the primitive nucleus. The nucleus of Giardia lamblia , a parasitic archezoan, was investigated in the present work. The dense aggregates of heterochromatin of Giardia lamblia , which might be mistaken as nucleolus, could occasionally be observed in nucleu ...
... nucleus may express some characteristics of the primitive nucleus. The nucleus of Giardia lamblia , a parasitic archezoan, was investigated in the present work. The dense aggregates of heterochromatin of Giardia lamblia , which might be mistaken as nucleolus, could occasionally be observed in nucleu ...
Statistical Analysis of the Patterns of Spore Formation
... occurs at a critical point 15to 20 min into a round of chromosome replication (Mandelstam & Higgs, 1974). Now, if there is an immediate response to resuspension, that is necessary for sporulation, this response cannot be equated with ‘initiation’ (as used by Mandelstam & Higgs, 1974). However, recen ...
... occurs at a critical point 15to 20 min into a round of chromosome replication (Mandelstam & Higgs, 1974). Now, if there is an immediate response to resuspension, that is necessary for sporulation, this response cannot be equated with ‘initiation’ (as used by Mandelstam & Higgs, 1974). However, recen ...
Beyond apoptosis: nonapoptotic cell death in physiology and disease
... alike, but are distinct from apoptosis, and are probably caused by different mechanisms (Jaattela and Tschopp 2003). In particular, at late stages of both accidental necrosis and necrosis-like PCD, nuclear morphology is similar and is characterized by the absence of apoptotic features. However, the ...
... alike, but are distinct from apoptosis, and are probably caused by different mechanisms (Jaattela and Tschopp 2003). In particular, at late stages of both accidental necrosis and necrosis-like PCD, nuclear morphology is similar and is characterized by the absence of apoptotic features. However, the ...
Bio 226: Cell and Molecular Biology
... • Periclinal = perpendicular to surface: get longer • Anticlinal = parallel to surface: add more layers Now must decide which way to elongate: which walls to stretch ...
... • Periclinal = perpendicular to surface: get longer • Anticlinal = parallel to surface: add more layers Now must decide which way to elongate: which walls to stretch ...
Name - Wsfcs
... kókkos, grain, and seed) or rod-shaped, called bacilli (sing. bacillus, from Latin baculus, stick). Elongation is associated with swimming. Some rod-shaped bacteria, called vibrio, are slightly curved or comma-shaped; others, can be spiral-shaped, called spirilla, or tightly coiled, called spirochae ...
... kókkos, grain, and seed) or rod-shaped, called bacilli (sing. bacillus, from Latin baculus, stick). Elongation is associated with swimming. Some rod-shaped bacteria, called vibrio, are slightly curved or comma-shaped; others, can be spiral-shaped, called spirilla, or tightly coiled, called spirochae ...
Geometrical constraints in the scaling relationships between
... and indirectly, through limits for the transport of material to the cell and within the cell or through the cell metabolism. As all these constraints are possibly bidirectional, we explicitly do not assume any cause-and-effect chain and limit ourselves to symmetrical scaling relationships. The simpl ...
... and indirectly, through limits for the transport of material to the cell and within the cell or through the cell metabolism. As all these constraints are possibly bidirectional, we explicitly do not assume any cause-and-effect chain and limit ourselves to symmetrical scaling relationships. The simpl ...
the Cytoskeleton in Plant Development1
... division changes, this change is always predicted by a corresponding localization of the preprophase band (reviewed in Wick, 199111421). The division spindle is always laid down per- ...
... division changes, this change is always predicted by a corresponding localization of the preprophase band (reviewed in Wick, 199111421). The division spindle is always laid down per- ...
Imaging the fate of histone Cse4 reveals de novo replacement in S
... Before a cell divides, it must create an exact copy of each of its chromosomes. The two copies of the chromosome are linked together at a region called the centromere. To separate them, structures called microtubules attach to each side of the centromere via a structure called the kinetochore. The k ...
... Before a cell divides, it must create an exact copy of each of its chromosomes. The two copies of the chromosome are linked together at a region called the centromere. To separate them, structures called microtubules attach to each side of the centromere via a structure called the kinetochore. The k ...
Opposite Polarity of Virus Budding and of Viral Envelope
... two viruses or to cell type differences in the sorting machinery. To answer this question it was necessary to study the sorting ofboth viruses in the two different epithelial cell systems. Since MDCK cells were not susceptible to SV infection, we compared the polarized budding ofSV and SFV in FRT ce ...
... two viruses or to cell type differences in the sorting machinery. To answer this question it was necessary to study the sorting ofboth viruses in the two different epithelial cell systems. Since MDCK cells were not susceptible to SV infection, we compared the polarized budding ofSV and SFV in FRT ce ...
In Vitro Cytotoxicity of Oxide Nanoparticles: Comparison
... FIGURE 5. Morphological changes of MSTO cell cultures after 6 days incubation: (A) control cells without treatment; (B) 7.5 ppm crocidolite (see arrow for a single fiber), enlargement of a single cell which has increased in size, fibers surrounding the cell; (C) 7.5 ppm ZrO2, no visible changes in ...
... FIGURE 5. Morphological changes of MSTO cell cultures after 6 days incubation: (A) control cells without treatment; (B) 7.5 ppm crocidolite (see arrow for a single fiber), enlargement of a single cell which has increased in size, fibers surrounding the cell; (C) 7.5 ppm ZrO2, no visible changes in ...
Discovery and Characterization of Novel Small Molecule Inhibitors
... washed three times in ice-cold phosphate-buffered saline supplemented with protease and phosphatase inhibitors. Immunocomplexes were boiled in SDS electrophoresis loading buffer and supernatants were resolved on a 12% Tris-glycine gel. Proteins were transferred to a nitrocellulose membrane and blott ...
... washed three times in ice-cold phosphate-buffered saline supplemented with protease and phosphatase inhibitors. Immunocomplexes were boiled in SDS electrophoresis loading buffer and supernatants were resolved on a 12% Tris-glycine gel. Proteins were transferred to a nitrocellulose membrane and blott ...
NEK1 Facilitates Cohesin Removal during Mammalian
... Meiosis is a specialized form of cell division that is highly conserved from fungi to humans; beginning with one round of pre-meiotic replication, followed by two rounds of division, to produce haploid gametes for sexual reproduction. The defining stage of meiosis, prophase I [1] encompasses two cri ...
... Meiosis is a specialized form of cell division that is highly conserved from fungi to humans; beginning with one round of pre-meiotic replication, followed by two rounds of division, to produce haploid gametes for sexual reproduction. The defining stage of meiosis, prophase I [1] encompasses two cri ...
Tissue Engineering - Gateway Coalition
... minimize the use of animals, using animal models only when necessary and at a later stage of material development. ...
... minimize the use of animals, using animal models only when necessary and at a later stage of material development. ...
Mitosis
Mitosis is a part of the cell cycle in which chromosomes in a cell nucleus are separated into two identical sets of chromosomes, each in its own nucleus. In general, mitosis (division of the nucleus) is often followed by cytokinesis, which divides the cytoplasm, organelles and cell membrane into two new cells containing roughly equal shares of these cellular components. Mitosis and cytokinesis together define the mitotic (M) phase of an animal cell cycle—the division of the mother cell into two daughter cells, genetically identical to each other and to their parent cell.The process of mitosis is divided into stages corresponding to the completion of one set of activities and the start of the next. These stages are prophase, prometaphase, metaphase, anaphase, and telophase. During mitosis, the chromosomes, which have already duplicated, condense and attach to fibers that pull one copy of each chromosome to opposite sides of the cell. The result is two genetically identical daughter nuclei. The cell may then divide by cytokinesis to produce two daughter cells. Producing three or more daughter cells instead of normal two is a mitotic error called tripolar mitosis or multipolar mitosis (direct cell triplication / multiplication). Other errors during mitosis can induce apoptosis (programmed cell death) or cause mutations. Certain types of cancer can arise from such mutations.Mitosis occurs only in eukaryotic cells and the process varies in different organisms. For example, animals undergo an ""open"" mitosis, where the nuclear envelope breaks down before the chromosomes separate, while fungi undergo a ""closed"" mitosis, where chromosomes divide within an intact cell nucleus. Furthermore, most animal cells undergo a shape change, known as mitotic cell rounding, to adopt a near spherical morphology at the start of mitosis. Prokaryotic cells, which lack a nucleus, divide by a different process called binary fission.