KEY
... of N2 that it reduces to ammonia. Yet even this large input underestimates the total energetic investment in nitrogen fixation. Briefly explain why merely counting the ATPs consumed underestimates the overall energetic cost of nitrogen fixation. The 8 electrons used to reduce N2 (and H+) are derived ...
... of N2 that it reduces to ammonia. Yet even this large input underestimates the total energetic investment in nitrogen fixation. Briefly explain why merely counting the ATPs consumed underestimates the overall energetic cost of nitrogen fixation. The 8 electrons used to reduce N2 (and H+) are derived ...
Title - Iowa State University
... a. Pyruvate is processed to release one molecule of carbon dioxide, and the remaining carbons are used to form acetyl CoA. b. One molecule of glucose is broken into two molecules of pyruvate, ATP is produced from ADP, and NAD+ is reduced to form NADH. c. Acetyl CoA is oxidized to two molecules of ca ...
... a. Pyruvate is processed to release one molecule of carbon dioxide, and the remaining carbons are used to form acetyl CoA. b. One molecule of glucose is broken into two molecules of pyruvate, ATP is produced from ADP, and NAD+ is reduced to form NADH. c. Acetyl CoA is oxidized to two molecules of ca ...
Using sunlight to make food (sugar) 6CO2 +
... They are used in the dark reaction which takes place in the stroma ...
... They are used in the dark reaction which takes place in the stroma ...
This paper is published in a part-themed issue of Photochemical
... removing air1 . Besides luciferin and luciferase, O2 is required for all bioluminescence to occur.7 While at Princeton Harvey accepted William McElroy as a PhD student and this would represent the start of a life-long study of firefly bioluminescence. McElroy’s research was seminal for a large number ...
... removing air1 . Besides luciferin and luciferase, O2 is required for all bioluminescence to occur.7 While at Princeton Harvey accepted William McElroy as a PhD student and this would represent the start of a life-long study of firefly bioluminescence. McElroy’s research was seminal for a large number ...
Title - Iowa State University
... certain cell types and organs? - Hormones act only on cells that express the appropriate receptor. - Target cells respond to a particular hormone because they contain a receptor for that hormone. 31. How does a cell being able to respond to a signal affect the regulation of gene expression? - A horm ...
... certain cell types and organs? - Hormones act only on cells that express the appropriate receptor. - Target cells respond to a particular hormone because they contain a receptor for that hormone. 31. How does a cell being able to respond to a signal affect the regulation of gene expression? - A horm ...
LOYOLA COLLEGE (AUTONOMOUS), CHENNAI – 600 034
... 6. Primary metabolism synthesizes building block metabolite and energy in uniform amounts. 7. The fluxes in and out of the Black box model are given by a constant rate and a variable substrate and product rate. 8. Recombinant PCR cannot be used for making novel proteins. 9. Strongly weak nodes are i ...
... 6. Primary metabolism synthesizes building block metabolite and energy in uniform amounts. 7. The fluxes in and out of the Black box model are given by a constant rate and a variable substrate and product rate. 8. Recombinant PCR cannot be used for making novel proteins. 9. Strongly weak nodes are i ...
STEP Experience Reflection – Chelsey Williams
... MicroRNA 122 (miR-122) is the most abundant liver- specific microRNA. After undergoing cellular processing, it is expressed during late stages of embryonic development. In the laboratory of Dr. Jacob and Dr. Ghoshal, miR-122 was found to be reduced in both rodent and human HCCs. Furthermore, loss of ...
... MicroRNA 122 (miR-122) is the most abundant liver- specific microRNA. After undergoing cellular processing, it is expressed during late stages of embryonic development. In the laboratory of Dr. Jacob and Dr. Ghoshal, miR-122 was found to be reduced in both rodent and human HCCs. Furthermore, loss of ...
ENZYME
... Used for treating arthiritis When used in conjunction with trypsin and rutin is as effective as prescribed analgesics in the osteoarthiritis management. ...
... Used for treating arthiritis When used in conjunction with trypsin and rutin is as effective as prescribed analgesics in the osteoarthiritis management. ...
Test Review Guide ch. 7, 9, 10
... 9. Name three different carbon products made from pyruvate 10. The first chemical reaction in the Krebs cycle is ____ 11. The final energy products (and number) of each turn of the Krebs Cycle. 12.How many NADHS, FADH2, ATP are produced in the Krebs cycle? 13. Where is phosphorylation reaction subs ...
... 9. Name three different carbon products made from pyruvate 10. The first chemical reaction in the Krebs cycle is ____ 11. The final energy products (and number) of each turn of the Krebs Cycle. 12.How many NADHS, FADH2, ATP are produced in the Krebs cycle? 13. Where is phosphorylation reaction subs ...
Expediting Purification of Cellular Proteins
... from a complex mixture is one of the standard methods for the detection of proteinprotein interactions. To circumvent the need for specific antibodies in affinity purification and subsequent detection, proteins can be expressed in fusion with a tag, i.e., an extension that has a high-affinity bindin ...
... from a complex mixture is one of the standard methods for the detection of proteinprotein interactions. To circumvent the need for specific antibodies in affinity purification and subsequent detection, proteins can be expressed in fusion with a tag, i.e., an extension that has a high-affinity bindin ...
Bacterial and Firefly Luciferase Genes in Transgenic Plants
... a complex with luciferase and, by energy transfer from the flavin to the secondary emitter 6,7-dimethyl-8-(l1D-ribityll-lumazine, blue light (475 nm) is emitted [Lee et al., 19891. In Vibrio fischeri strain Y-1 energy transfer occurs from excited flavin intermediates to a flavincontaining secondary ...
... a complex with luciferase and, by energy transfer from the flavin to the secondary emitter 6,7-dimethyl-8-(l1D-ribityll-lumazine, blue light (475 nm) is emitted [Lee et al., 19891. In Vibrio fischeri strain Y-1 energy transfer occurs from excited flavin intermediates to a flavincontaining secondary ...
Random Mutagenesis of Luciola mingrelica Firefly Luciferase
... click-beetles and some other species with λmax and spectral shape virtually unchanged upon decreasing pH, ii) pH-sensitive firefly luciferases, whose λmax shifts from 540-570 nm (green or yellow-green light) at pH 8.0 to ~620 nm (red light) at pH 6. This significant shift is usually explained by t ...
... click-beetles and some other species with λmax and spectral shape virtually unchanged upon decreasing pH, ii) pH-sensitive firefly luciferases, whose λmax shifts from 540-570 nm (green or yellow-green light) at pH 8.0 to ~620 nm (red light) at pH 6. This significant shift is usually explained by t ...
Mammalian two-hybrid (M2H) and co-immunoprecipitation (co
... SCN5A was cloned into pCMV-AD vector. The two plasmids and pFR-Luc reporter plasmid (0.25ug) were co-transfected into HEK293 cells using lipofectamine 2000 at different concentration (0.01ug, 0.1ug, 0.5ug, respectively). pBD-p53 +pAD-SV40T+ pFR-Luc reporter plasmid were used for interaction positive ...
... SCN5A was cloned into pCMV-AD vector. The two plasmids and pFR-Luc reporter plasmid (0.25ug) were co-transfected into HEK293 cells using lipofectamine 2000 at different concentration (0.01ug, 0.1ug, 0.5ug, respectively). pBD-p53 +pAD-SV40T+ pFR-Luc reporter plasmid were used for interaction positive ...
GREEN FLUORESCENT PROTEIN (GFP) GFP is produced by the
... occasionally the proton does not return to the chromophore, so the neutral chromophore is photoisomerized to the anionic form. Wild-type GFP folds fairly efficiently when expressed at or below room temperature, but its folding efficiency declines steeply at higher temperatures. Presumably this natur ...
... occasionally the proton does not return to the chromophore, so the neutral chromophore is photoisomerized to the anionic form. Wild-type GFP folds fairly efficiently when expressed at or below room temperature, but its folding efficiency declines steeply at higher temperatures. Presumably this natur ...
Answers for extension worksheet – Option C
... The shape and bonding between enzyme and substrate are specific. If the substrate is not the correct shape and does not have appropriate charges on its molecule, it will not bind to the active site and the reaction will not proceed. ...
... The shape and bonding between enzyme and substrate are specific. If the substrate is not the correct shape and does not have appropriate charges on its molecule, it will not bind to the active site and the reaction will not proceed. ...
Bioluminescence
... • Where the sunlight is dim, it can be used as camouflage. • Many small plankton use flashes of light to startle their predators in an attempt to interrupt their feeding. ...
... • Where the sunlight is dim, it can be used as camouflage. • Many small plankton use flashes of light to startle their predators in an attempt to interrupt their feeding. ...
N-Terminal Intramolecularly Conserved Histidines of Three Domains
... their pH-activity profiles mimicked that of the proteolytic fragments reported earlier. Replacement of N-terminal histidines by alanine by site-directed mutagenesis identified four that are involved in the loss of activity at high pH. This system illustrates an unusual, possibly unique mechanism for ...
... their pH-activity profiles mimicked that of the proteolytic fragments reported earlier. Replacement of N-terminal histidines by alanine by site-directed mutagenesis identified four that are involved in the loss of activity at high pH. This system illustrates an unusual, possibly unique mechanism for ...
Reporter genes
... into the culture medium by transfected cells. The hGH from the supernatant of the culture medium binds to the antibody on the plate. Subsequently, the bound hGH is detected in two steps via a digoxigenincoupled anti-hGH antibody and a peroxidase-coupled anti-digoxigenin ...
... into the culture medium by transfected cells. The hGH from the supernatant of the culture medium binds to the antibody on the plate. Subsequently, the bound hGH is detected in two steps via a digoxigenincoupled anti-hGH antibody and a peroxidase-coupled anti-digoxigenin ...
GFP
... into the culture medium by transfected cells. The hGH from the supernatant of the culture medium binds to the antibody on the plate. Subsequently, the bound hGH is detected in two steps via a digoxigenincoupled anti-hGH antibody and a peroxidase-coupled anti-digoxigenin ...
... into the culture medium by transfected cells. The hGH from the supernatant of the culture medium binds to the antibody on the plate. Subsequently, the bound hGH is detected in two steps via a digoxigenincoupled anti-hGH antibody and a peroxidase-coupled anti-digoxigenin ...
Presentation
... to characterize influence of viscous media on the binding characteristics of bacterial luciferase by steady-state and timeresolved fluorescence. Fig. 2 - Native and unfolded conformation of the protein in the presence of osmolytes. [C. Le Coeur at al., Life Sciences and Biology, 2005] ...
... to characterize influence of viscous media on the binding characteristics of bacterial luciferase by steady-state and timeresolved fluorescence. Fig. 2 - Native and unfolded conformation of the protein in the presence of osmolytes. [C. Le Coeur at al., Life Sciences and Biology, 2005] ...
Bioluminescence: Luciferin-Lucferase ATP Detection Assay
... macroscopic measurements are the foundation for most HTS methods, which rely heavily on the use of multi• Light must be absorbed by across well plates to rapidly measure a single parameter a large number of samples. Furthermore, fluorescent asfluorophore tothereach excited state says can also be aff ...
... macroscopic measurements are the foundation for most HTS methods, which rely heavily on the use of multi• Light must be absorbed by across well plates to rapidly measure a single parameter a large number of samples. Furthermore, fluorescent asfluorophore tothereach excited state says can also be aff ...
Supplementary Notes - Word file
... Wild-type (T217I) and mutant luciferases were produced in Escherichia coli HB101 cells. The recombinant E. coli cells producing the enzymes were grown aerobically at 30˚C for 18 h in LB medium containing 0.1 mg/ml ampicillin. The cells harvested from a 4-L culture were disrupted by sonication. The c ...
... Wild-type (T217I) and mutant luciferases were produced in Escherichia coli HB101 cells. The recombinant E. coli cells producing the enzymes were grown aerobically at 30˚C for 18 h in LB medium containing 0.1 mg/ml ampicillin. The cells harvested from a 4-L culture were disrupted by sonication. The c ...
Bioluminescence
... Chemistry: Imidazolopyrazinone Found in all tissues of the animal Highest concentration in digestive gland, liver hepatopancreas, organs with high levels of oxidative reactions ...
... Chemistry: Imidazolopyrazinone Found in all tissues of the animal Highest concentration in digestive gland, liver hepatopancreas, organs with high levels of oxidative reactions ...
Luciferase
Luciferase is a generic term for the class of oxidative enzymes used in bioluminescence and is distinct from a photoprotein. The name is derived from Lucifer, the root of which means 'light-bearer' (lucem ferre). One example is the firefly luciferase (EC 1.13.12.7) from the firefly Photinus pyralis. ""Firefly luciferase"" as a laboratory reagent often refers to P. pyralis luciferase although recombinant luciferases from several other species of fireflies are also commercially available.