A Novel Chimeric Low-Molecular-Weight Glutenin
... were encoded by the complex Glu-3 loci on the short arms of homeologous group 1 chromosomes in hexaploid wheat (Singh and Shepherd 1988; Gupta and Shepherd 1990; Yan et al. 1999). In the past, an increasing number of LMW-GS genes have been isolated and characterized from common wheat (D’Ovidio and M ...
... were encoded by the complex Glu-3 loci on the short arms of homeologous group 1 chromosomes in hexaploid wheat (Singh and Shepherd 1988; Gupta and Shepherd 1990; Yan et al. 1999). In the past, an increasing number of LMW-GS genes have been isolated and characterized from common wheat (D’Ovidio and M ...
IGV Handout file
... The Integrative Genomics Viewer is a visualization tool for exploring and analyzing large genome datasets. It is a lightweight genomic data viewer on which you can work with prebuilt genomes or load any genome that you want. It may be used for viewing a variety of data such as expression data, NGS a ...
... The Integrative Genomics Viewer is a visualization tool for exploring and analyzing large genome datasets. It is a lightweight genomic data viewer on which you can work with prebuilt genomes or load any genome that you want. It may be used for viewing a variety of data such as expression data, NGS a ...
Lecture 7 - Pitt CPATH Project
... Sometimes you have not find any similarity If so: change the expect value to 1000 or even larger and click “BLAST”, keep raising the expect value until you get hits, then evaluate hits by position • This will show very weak similarity which can be better ...
... Sometimes you have not find any similarity If so: change the expect value to 1000 or even larger and click “BLAST”, keep raising the expect value until you get hits, then evaluate hits by position • This will show very weak similarity which can be better ...
Draft of first homework
... Many students thought that tiling array probes are better if they overlap mRNAs (and, conversely, that probes are bad if they are not overlapping mRNAs). This is not true, as the whole point with a tiling array is to be unbiased in terms of annotation, so that you can see any mRNAs, including unknow ...
... Many students thought that tiling array probes are better if they overlap mRNAs (and, conversely, that probes are bad if they are not overlapping mRNAs). This is not true, as the whole point with a tiling array is to be unbiased in terms of annotation, so that you can see any mRNAs, including unknow ...
General background text Pharmacogenetics - CYP3A4
... the functionality of a protein (for example the enzyme or the receptor), but also the physical manifestation of a disease. The phenotype is a result of the genotype that a person possesses, the degree of expression of the gene in question and the combination with environmental factors such as co-med ...
... the functionality of a protein (for example the enzyme or the receptor), but also the physical manifestation of a disease. The phenotype is a result of the genotype that a person possesses, the degree of expression of the gene in question and the combination with environmental factors such as co-med ...
Seed Germination Multiplexed Quantitative Gene Expression
... analyzing a single gene at a time using SYBR-green detection. Real-time reverse-transcription quantitative PCR (real-time qPCR) is widely used for gene expression, having replaced Northern blots as the preferred method by which gene expression is quantified. Real-time qPCR has proven to be reproduci ...
... analyzing a single gene at a time using SYBR-green detection. Real-time reverse-transcription quantitative PCR (real-time qPCR) is widely used for gene expression, having replaced Northern blots as the preferred method by which gene expression is quantified. Real-time qPCR has proven to be reproduci ...
Recombinant DNA WS
... 1. Sequence each event of creating protein using recombinant DNA in the correct order. Use the word bank to fill in the blanks in the sequencing cutouts. 2. Draw a picture to match the process, making sure to label your drawing with the underlined words from the cut-outs. Use a different color to re ...
... 1. Sequence each event of creating protein using recombinant DNA in the correct order. Use the word bank to fill in the blanks in the sequencing cutouts. 2. Draw a picture to match the process, making sure to label your drawing with the underlined words from the cut-outs. Use a different color to re ...
CH 15 Chromosomal Basis of Inheritance Mendelian
... Morgan discovered that genes can be linked, but the linkage was incomplete, because some recombinant phenotypes were observed. He proposed that some process must occasionally break the physical connection between genes on the same chromosome. That mechanism was the crossing over of homologous chromo ...
... Morgan discovered that genes can be linked, but the linkage was incomplete, because some recombinant phenotypes were observed. He proposed that some process must occasionally break the physical connection between genes on the same chromosome. That mechanism was the crossing over of homologous chromo ...
Limitations of Pseudogenes in Identifying Gene Losses
... the v5.3 genome assembly and annotation to determine if any of these potential gene losses are simply due to gaps in the v4.3 assembly. The 114 query sequences were searched against the v5.3 D. melanogaster genome using BLASTn with the same criteria as before. Interestingly, 23 query genes hit very ...
... the v5.3 genome assembly and annotation to determine if any of these potential gene losses are simply due to gaps in the v4.3 assembly. The 114 query sequences were searched against the v5.3 D. melanogaster genome using BLASTn with the same criteria as before. Interestingly, 23 query genes hit very ...
The genomes of four tapeworm species reveal adaptations to
... One chromosome is complete from telomere to telomere, and 13 of the expected 18 telomeres are joined to scaffolds (Fig. 1a). This quality and completeness is comparable to that of the first publications of Caenorhabditis elegans and Drosophila melanogaster genomes8,9. The 115- to 141-megabase (Mb) n ...
... One chromosome is complete from telomere to telomere, and 13 of the expected 18 telomeres are joined to scaffolds (Fig. 1a). This quality and completeness is comparable to that of the first publications of Caenorhabditis elegans and Drosophila melanogaster genomes8,9. The 115- to 141-megabase (Mb) n ...
Yeast genome evolution-the origin of the species
... genes in yeast genomes are truly redundant. If any such genes existed, there would be no advantage to maintaining them and hence no selection against losing them, so we would expect them to have been mutated and lost from genomes long ago. Genes with no obvious phenotype may nevertheless make a smal ...
... genes in yeast genomes are truly redundant. If any such genes existed, there would be no advantage to maintaining them and hence no selection against losing them, so we would expect them to have been mutated and lost from genomes long ago. Genes with no obvious phenotype may nevertheless make a smal ...
Single-step generation of rabbits carrying a targeted allele of the
... capacity to accomplish gene targeting with high accuracy. Although no transgene was detected in our experiments, fewer than 5% of the genetically targeted offspring expressed the hCas9 transgene in the mouse system [12]. Moreover, compared with mouse data in general (more than 10% of genetically mod ...
... capacity to accomplish gene targeting with high accuracy. Although no transgene was detected in our experiments, fewer than 5% of the genetically targeted offspring expressed the hCas9 transgene in the mouse system [12]. Moreover, compared with mouse data in general (more than 10% of genetically mod ...
AACL BIOFLUX
... than the larger wild type. So blond is not a mutation directly affecting melanin sythesis. It is because the melanophores are so small in size and number that blond guppies look less dark than their wildtype counterparts. What we found fascinating in the Goodrich et al 1944 paper is the speculation ...
... than the larger wild type. So blond is not a mutation directly affecting melanin sythesis. It is because the melanophores are so small in size and number that blond guppies look less dark than their wildtype counterparts. What we found fascinating in the Goodrich et al 1944 paper is the speculation ...
Gene Expression of Heart and Adipocyte Fatty Acid
... quantitative reverse transcription polymerase-chain reaction (FQ-RT-PCR). The primers were designed according to the sequences of HFABP, A-FABP and GAPDH genes in Gallus gallus, which were used as target genes and internal reference gene, respectively. The levels of H-FABP and A-FABP gene expression ...
... quantitative reverse transcription polymerase-chain reaction (FQ-RT-PCR). The primers were designed according to the sequences of HFABP, A-FABP and GAPDH genes in Gallus gallus, which were used as target genes and internal reference gene, respectively. The levels of H-FABP and A-FABP gene expression ...
Part 2 - Laboratory of Aquaculture & Artemia Reference Center
... On going proteomic research at IART in Artemia on ...
... On going proteomic research at IART in Artemia on ...
Journal of Biotechnology
... gaps revealed that most of the gaps (25 of 34) between scaffolded contigs were relatively small (3 ± 10 bp). They seem to be due to inverted repeats forming stable secondary structures which might result in no or poor amplification during the various PCR steps involved in library preparation and NGS ...
... gaps revealed that most of the gaps (25 of 34) between scaffolded contigs were relatively small (3 ± 10 bp). They seem to be due to inverted repeats forming stable secondary structures which might result in no or poor amplification during the various PCR steps involved in library preparation and NGS ...
The Ensembl Database
... Nonetheless, this is useful for finding putative orthologs and for discovering regulatory regions using multiple sequence alignments ...
... Nonetheless, this is useful for finding putative orthologs and for discovering regulatory regions using multiple sequence alignments ...
Justification of Size Estimates for Tomato Genome Sequencing
... Additional Information. When the sequencing project is advanced to the stage where BAC contigs can be assayed for both total non-redundant sequence length and physical distance based on in situ hybridization, we will be able to develop an additional estimate of euchromatin physical size through vali ...
... Additional Information. When the sequencing project is advanced to the stage where BAC contigs can be assayed for both total non-redundant sequence length and physical distance based on in situ hybridization, we will be able to develop an additional estimate of euchromatin physical size through vali ...
Microarray statistical validation and functional annotation
... Molecular Function: the tasks performed by individual gene, products; examples are transcription factor and DNA helicase. ...
... Molecular Function: the tasks performed by individual gene, products; examples are transcription factor and DNA helicase. ...
Making sense of genetic variation!
... Population genetics describes variation within and between species There are two major areas of interest: •!Describe degrees of genetic variation within and between individuals and/or population •!infer the evolutionary mechanisms responsible for the origins and maintenance of genetic variation Muta ...
... Population genetics describes variation within and between species There are two major areas of interest: •!Describe degrees of genetic variation within and between individuals and/or population •!infer the evolutionary mechanisms responsible for the origins and maintenance of genetic variation Muta ...
Copy-number variation
Copy-number variations (CNVs)—a form of structural variation—are alterations of the DNA of a genome that results in the cell having an abnormal or, for certain genes, a normal variation in the number of copies of one or more sections of the DNA. CNVs correspond to relatively large regions of the genome that have been deleted (fewer than the normal number) or duplicated (more than the normal number) on certain chromosomes. For example, the chromosome that normally has sections in order as A-B-C-D might instead have sections A-B-C-C-D (a duplication of ""C"") or A-B-D (a deletion of ""C"").This variation accounts for roughly 13% of human genomic DNA and each variation may range from about one kilobase (1,000 nucleotide bases) to several megabases in size. CNVs contrast with single-nucleotide polymorphisms (SNPs), which affect only one single nucleotide base.