Colony PCR - University of Illinois Crop Sciences
... Taq polymerase leaves an “A” overhang • Taq is the thermostable DNA polymerase from Thermus aquaticus we used for PCR. • When Taq synthesizes a new strand, it always puts an extra “A” at the end • This can be useful, but note: other polymerases do not do this, they leave “blunt” ends. Only Taq poly ...
... Taq polymerase leaves an “A” overhang • Taq is the thermostable DNA polymerase from Thermus aquaticus we used for PCR. • When Taq synthesizes a new strand, it always puts an extra “A” at the end • This can be useful, but note: other polymerases do not do this, they leave “blunt” ends. Only Taq poly ...
Diversity of Living Things
... • Cell wall • Nucleoid – chromosomal DNA • Plasmids – small circular pieces of DNA • Capsule – composed of polysaccharides, protects cell against phagocytosis ...
... • Cell wall • Nucleoid – chromosomal DNA • Plasmids – small circular pieces of DNA • Capsule – composed of polysaccharides, protects cell against phagocytosis ...
Biology 230 Microbiology - Harford Community College
... • All members of living world that are not prokaryotes are in domain eukarya • May be single celled or multi-cellular • Always contain true membrane-bound nucleus and other internal organelles • Far more complex than prokaryotes ...
... • All members of living world that are not prokaryotes are in domain eukarya • May be single celled or multi-cellular • Always contain true membrane-bound nucleus and other internal organelles • Far more complex than prokaryotes ...
Microlog Minutes/1
... Try initial isolation on plain BUG or TSA media and take the second subculture to BUG with blood. If the organism grows after first subculture on BUG w/ blood, but the growth is poor, let it grow enough to make sure you have a pure culture. Subculture a single colony using a swab that has been dippe ...
... Try initial isolation on plain BUG or TSA media and take the second subculture to BUG with blood. If the organism grows after first subculture on BUG w/ blood, but the growth is poor, let it grow enough to make sure you have a pure culture. Subculture a single colony using a swab that has been dippe ...
Summer Bio153S Lecture 7: prokaryotes
... • use energy from the sun but extract electrons from substances other than water • photosynthesis does not release oxygen • most species are strict anaerobes ...
... • use energy from the sun but extract electrons from substances other than water • photosynthesis does not release oxygen • most species are strict anaerobes ...
H2S production hydrogen sulfide production
... • Medium has the pH indicator – bromthymol blue • Green – neutral • Blue – basic • Yellow – acidic • When bacteria use citrate ammonium ions are released. • Environment becomes basic - blue ...
... • Medium has the pH indicator – bromthymol blue • Green – neutral • Blue – basic • Yellow – acidic • When bacteria use citrate ammonium ions are released. • Environment becomes basic - blue ...
mrsa - Bergen.org
... A: Peptidoglycan layer Polymer of sugars and amino acids for structure and support. Note the difference in thickness between the two cells. This difference is what allows gram-positive and gram-negative bacteria to stain in separate colors. B: Cytoplasmic Membrane Encases the cell’s cytoplasm. C ...
... A: Peptidoglycan layer Polymer of sugars and amino acids for structure and support. Note the difference in thickness between the two cells. This difference is what allows gram-positive and gram-negative bacteria to stain in separate colors. B: Cytoplasmic Membrane Encases the cell’s cytoplasm. C ...
LECTURE 12 THE BACTERIA
... • Deinococcus can survive extremely high levels of UV and Gamma radiation. Is Gm- but outer membrane is surrounded by a protein sheath (S-layer) thus it stains Gm+. Has several copies of chromosome per cell and is very efficient at DNA repair….. ...
... • Deinococcus can survive extremely high levels of UV and Gamma radiation. Is Gm- but outer membrane is surrounded by a protein sheath (S-layer) thus it stains Gm+. Has several copies of chromosome per cell and is very efficient at DNA repair….. ...
No Slide Title
... PG is a complex sugar and peptide structure important for cell wall stability and shape. ...
... PG is a complex sugar and peptide structure important for cell wall stability and shape. ...
Bacteria Quiz - NagelBeelmanScience
... ____3. The process where one bacterium transfers its genes to another cell over a “bridge” is called A. conjugation B. decomposer C. binary fission D. respiration ____4. Organisms that break down large chemicals from dead organisms into small chemicals are called A. conjugation B. decomposer C. bina ...
... ____3. The process where one bacterium transfers its genes to another cell over a “bridge” is called A. conjugation B. decomposer C. binary fission D. respiration ____4. Organisms that break down large chemicals from dead organisms into small chemicals are called A. conjugation B. decomposer C. bina ...
OR208 The gut microbiota of termites: evolutionary origin and
... symbionts that specifically colonize the flagellates. These flagellate symbionts were recruited among the gut bacteria and apparently serve to complement deficits in the nitrogen metabolism of their flagellate hosts. When the flagellates were lost in higher termites (family Termitidae), the availabi ...
... symbionts that specifically colonize the flagellates. These flagellate symbionts were recruited among the gut bacteria and apparently serve to complement deficits in the nitrogen metabolism of their flagellate hosts. When the flagellates were lost in higher termites (family Termitidae), the availabi ...
Using serial dilutions and plating to establish viable bacterial cell
... microorganisms using a haemocytometer, or by serial diluting the bacteria and plating the diluted bacteria on media that supports the growth of the micro-organisms. The latter method is somewhat more time consuming, but provides statistically accurate and repeatable results. This method is also the ...
... microorganisms using a haemocytometer, or by serial diluting the bacteria and plating the diluted bacteria on media that supports the growth of the micro-organisms. The latter method is somewhat more time consuming, but provides statistically accurate and repeatable results. This method is also the ...
Chapter 6
... » Protozoans – ciliates, choanoflagellates, amoeboids (foraminiferans, radiolarians) ...
... » Protozoans – ciliates, choanoflagellates, amoeboids (foraminiferans, radiolarians) ...
Chapter 27
... Spontaneous mutations may occur in a single gene at an average rate of one in ten million per cell division in E. coli. It has been suggested that about 9 million new mutations arise per day in the E. coli found in the human intestine. New mutations, though individually rare, can greatly increase ge ...
... Spontaneous mutations may occur in a single gene at an average rate of one in ten million per cell division in E. coli. It has been suggested that about 9 million new mutations arise per day in the E. coli found in the human intestine. New mutations, though individually rare, can greatly increase ge ...
Microbial culture and growth - Microbiology and Molecular Genetics
... In a continuous culture, all cells in a population achieve a steady state, which allows detailed study of bacterial physiology. The chemostat ensures logarithmic growth by constantly adding and removing equal amounts of culture media. Note that the human gastrointestinal tract is engineered much lik ...
... In a continuous culture, all cells in a population achieve a steady state, which allows detailed study of bacterial physiology. The chemostat ensures logarithmic growth by constantly adding and removing equal amounts of culture media. Note that the human gastrointestinal tract is engineered much lik ...
Veterinary Bacteriology and Virology 101
... Excessive clotting causes blocked capillaries Organ failure begins with cardiovascular and respiratory failure, followed by additional organ failure. ...
... Excessive clotting causes blocked capillaries Organ failure begins with cardiovascular and respiratory failure, followed by additional organ failure. ...
Chapter 27 - cloudfront.net
... 19. List the major groups of bacteria, describe their mode of nutrition, some characteristic features and representative examples. ...
... 19. List the major groups of bacteria, describe their mode of nutrition, some characteristic features and representative examples. ...
Food preservation - Eduspace
... Reduction of pH value in situ by microbial action and sometimes additional inhibition by the lactic and acetic acids formed and by other microbial products. (e.g. ethanol,bacteriocins) ...
... Reduction of pH value in situ by microbial action and sometimes additional inhibition by the lactic and acetic acids formed and by other microbial products. (e.g. ethanol,bacteriocins) ...
Notes are available
... 1. Conjugation occurs when a bacterium passes DNA to a second bacterium through a tube (sex pilus) that temporarily joins two cells; occurs only between bacteria in same or closely related species. 2. Transformation involves bacteria taking up free pieces of DNA secreted by live bacteria or released ...
... 1. Conjugation occurs when a bacterium passes DNA to a second bacterium through a tube (sex pilus) that temporarily joins two cells; occurs only between bacteria in same or closely related species. 2. Transformation involves bacteria taking up free pieces of DNA secreted by live bacteria or released ...
Micro-Ch10-13_B.pdf
... 67) Specialized transduction differs from generalized transduction in that specialized transduction A) Transfers specific DNA. B) Kills the host. C) Involves lysogeny. D) Transfers DNA from one cell to another. E) None of the above. 68) A viral species is a group of viruses that A) Have the same mor ...
... 67) Specialized transduction differs from generalized transduction in that specialized transduction A) Transfers specific DNA. B) Kills the host. C) Involves lysogeny. D) Transfers DNA from one cell to another. E) None of the above. 68) A viral species is a group of viruses that A) Have the same mor ...
Bacterial Cell Structure, Function and Classification
... such as slime in sink drains and toilets, but they are also responsible for many types of more serious infections. Dental plaque is the result of biofilm formation. Patients who receive implants (artificial hips or heart valves) or have in-dwelling devices such as venous or urinary catheters may get ...
... such as slime in sink drains and toilets, but they are also responsible for many types of more serious infections. Dental plaque is the result of biofilm formation. Patients who receive implants (artificial hips or heart valves) or have in-dwelling devices such as venous or urinary catheters may get ...
Chapter 1: The Microbial World and You
... Antibiotic: Substance produced by a microorganism that in small amounts inhibits the growth of another microbe. Antibiotic producing microbes include: Gram-Positive Rods: Bacillus subtilis: Bacitracin Bacillus polymyxa: Polymyxin ...
... Antibiotic: Substance produced by a microorganism that in small amounts inhibits the growth of another microbe. Antibiotic producing microbes include: Gram-Positive Rods: Bacillus subtilis: Bacitracin Bacillus polymyxa: Polymyxin ...
Host Defense Against Bacterial Toxins: Mechanisms and Therapies
... Host Defense Against Bacterial Toxins: Mechanisms and Therapies Pore-forming toxins are the largest single class of proteinaceous bacterial toxins produced by both Gram-positive and Gram-negative bacterial species. Many pore-forming toxins have been proven to be key virulence factors in major pathog ...
... Host Defense Against Bacterial Toxins: Mechanisms and Therapies Pore-forming toxins are the largest single class of proteinaceous bacterial toxins produced by both Gram-positive and Gram-negative bacterial species. Many pore-forming toxins have been proven to be key virulence factors in major pathog ...
Bacterial Diseases of Plants
... occur singly or in colonies of cells. Bacteria are classified into two main groups based on cell wall structure, which can be determined by a simple staining procedure called the Gram stain. Gram negative bacteria stain red or pink and Gram positive bacteria stain purple. The difference in color is ...
... occur singly or in colonies of cells. Bacteria are classified into two main groups based on cell wall structure, which can be determined by a simple staining procedure called the Gram stain. Gram negative bacteria stain red or pink and Gram positive bacteria stain purple. The difference in color is ...