Role of cystinosin in vesicular trafficking and membrane fusion
... structures and a diminution of the usual pattern of small discrete intracytoplasmic vesicles characteristic of lysosomes. The number of these structures was drastically decreased when cystinosin C-terminal tail, its 5th inter-TM loop, or both motifs were altered. The enlarged lysosomes are reminisce ...
... structures and a diminution of the usual pattern of small discrete intracytoplasmic vesicles characteristic of lysosomes. The number of these structures was drastically decreased when cystinosin C-terminal tail, its 5th inter-TM loop, or both motifs were altered. The enlarged lysosomes are reminisce ...
Research Roundup - The Journal of Cell Biology
... within minutes. Proteasome levels then remained high in the spines for up to an hour. Ubiquitinated proteins, targeted for destruction, also initially increased, but shortly thereafter decreased as the proteasomes arrived and got to work. Thus, the study shows that proteasomes are dynamic machines t ...
... within minutes. Proteasome levels then remained high in the spines for up to an hour. Ubiquitinated proteins, targeted for destruction, also initially increased, but shortly thereafter decreased as the proteasomes arrived and got to work. Thus, the study shows that proteasomes are dynamic machines t ...
Type III Secretion System
... are important in TTSS by preventing premature interactions of the secreted factors with other proteins. Chaperones also ensure presecretory stabilization and efficient secretion Lack of specific chaperones can reduce the secretion of the protein due to degradation in the bacteria cytoplasm E.g ...
... are important in TTSS by preventing premature interactions of the secreted factors with other proteins. Chaperones also ensure presecretory stabilization and efficient secretion Lack of specific chaperones can reduce the secretion of the protein due to degradation in the bacteria cytoplasm E.g ...
Slide 1
... pseudosubstrate peptide allows the substrate specificity and inhibition by the R subunit to be understood as due to complementarity with specific residues in the c subunit. ...
... pseudosubstrate peptide allows the substrate specificity and inhibition by the R subunit to be understood as due to complementarity with specific residues in the c subunit. ...
Foreign Gene Expression and Protein Production
... • Prokaryotic promoter—ribosome binding site—MCS— transcription termination site • Prokaryotic selectable marker ...
... • Prokaryotic promoter—ribosome binding site—MCS— transcription termination site • Prokaryotic selectable marker ...
Lecture 16 Outline
... Cell Shape. Formation of Tubulin Filaments- Depends on tubulin dimer concentration High concentrations lead to growth or assemble, while Low concentrations lead to disassembly or depolymerization. Above Critical concentration (concentration of free tubulin dimers) assembly will occur, below- disasse ...
... Cell Shape. Formation of Tubulin Filaments- Depends on tubulin dimer concentration High concentrations lead to growth or assemble, while Low concentrations lead to disassembly or depolymerization. Above Critical concentration (concentration of free tubulin dimers) assembly will occur, below- disasse ...
Supplement_2_-_PLoS_
... C1q, mannose-binding lectin (MBL), and pulmonary surfactant protein A (SPA) interact with human monocytes and macrophages, resulting in the enhancement of phagocytosis of suboptimally opsonized targets. mAbs that recognize a cell surface molecule of 126,000 Mr, designated C1qRP, have been shown to i ...
... C1q, mannose-binding lectin (MBL), and pulmonary surfactant protein A (SPA) interact with human monocytes and macrophages, resulting in the enhancement of phagocytosis of suboptimally opsonized targets. mAbs that recognize a cell surface molecule of 126,000 Mr, designated C1qRP, have been shown to i ...
1. Overview
... • Coordinates can be extracted and viewed • Comparisons of structures allows identification of structural motifs • Proteins with similar functions and sequences = homologs ...
... • Coordinates can be extracted and viewed • Comparisons of structures allows identification of structural motifs • Proteins with similar functions and sequences = homologs ...
Protein Reading Questions Due Monday File
... 8. Explain the properties of the amino acid groups below, based on their R-group: a. Nonpolar side chains/Hydrophobic: b. Polar side chains/ Hydrophilic: c. Electrically charged side chains/Hydrophilic: 9. What are the bonds between amino acids in a polypeptide called AND what type of bond is it? ...
... 8. Explain the properties of the amino acid groups below, based on their R-group: a. Nonpolar side chains/Hydrophobic: b. Polar side chains/ Hydrophilic: c. Electrically charged side chains/Hydrophilic: 9. What are the bonds between amino acids in a polypeptide called AND what type of bond is it? ...
Enzyme-linked secondary antibodies
... The sensitivity of the Sandwich ELISA is dependent on four factors: ...
... The sensitivity of the Sandwich ELISA is dependent on four factors: ...
HSC70 (Hsp73) Protein (active) Catalog# SPR
... endoplasmic reticulum and the cytosol, as well as in bacteria. The genes show a high degree of conservation, having at least 5O% identity (2). The N-terminal two thirds of hsp70s are more conserved than the C-terminal third. Hsp70 binds ATP with high affinity and possesses a weak ATPase activity whi ...
... endoplasmic reticulum and the cytosol, as well as in bacteria. The genes show a high degree of conservation, having at least 5O% identity (2). The N-terminal two thirds of hsp70s are more conserved than the C-terminal third. Hsp70 binds ATP with high affinity and possesses a weak ATPase activity whi ...
Laura Bassi Centres of Expertise - PlantBioP Plant
... 10-20%. Demand for these products by far exceeds the production capacity and their cost-intensive processes, which are mainly based on mammalian cells, are an enormous hurdle hindering world-wide application of the drugs. New procedures for generating these biopharmaceutical products have been devel ...
... 10-20%. Demand for these products by far exceeds the production capacity and their cost-intensive processes, which are mainly based on mammalian cells, are an enormous hurdle hindering world-wide application of the drugs. New procedures for generating these biopharmaceutical products have been devel ...
Regulation
... leader region (region between operator and first structual gene). 162 nucleotides with a transcriptional termination site at 3' end. encodes for a short polypeptide chain (14 amino acid) which has two tryp codons. base pairing that can occur within leader region impact that specific base p ...
... leader region (region between operator and first structual gene). 162 nucleotides with a transcriptional termination site at 3' end. encodes for a short polypeptide chain (14 amino acid) which has two tryp codons. base pairing that can occur within leader region impact that specific base p ...
OriGene Technologies launches over 5,000 heavy isotope labeled
... company, has announced the first of its kind launch of over 5,000 heavy isotope labeled human proteins as internal standards for SRM/MRM (single reaction monitoring, multiple reaction monitoring) mass spectrometry analyses. The announcement was made at the 2010 American Society for Mass Spectrometry ...
... company, has announced the first of its kind launch of over 5,000 heavy isotope labeled human proteins as internal standards for SRM/MRM (single reaction monitoring, multiple reaction monitoring) mass spectrometry analyses. The announcement was made at the 2010 American Society for Mass Spectrometry ...
Proteins – Organic/Macromolecule #3
... Name:_____________________________________Date:________Per:_____ Proteins are organic molecules. They are built from the connection of many amino acids into a long chain. Proteins have many functions that can be remembered by this acronym STEM(Structure, Transport, Enzymes and Movement). Proteins pr ...
... Name:_____________________________________Date:________Per:_____ Proteins are organic molecules. They are built from the connection of many amino acids into a long chain. Proteins have many functions that can be remembered by this acronym STEM(Structure, Transport, Enzymes and Movement). Proteins pr ...
Proteins – Organic/Macromolecule #3
... Name:_____________________________________Date:________Per:_____ Proteins are organic molecules. They are built from the connection of many amino acids into a long chain. Proteins have many functions that can be remembered by this acronym STEM(Structure, Transport, Enzymes and Movement). Proteins pr ...
... Name:_____________________________________Date:________Per:_____ Proteins are organic molecules. They are built from the connection of many amino acids into a long chain. Proteins have many functions that can be remembered by this acronym STEM(Structure, Transport, Enzymes and Movement). Proteins pr ...
Identification of HLA-A*0201-Restricted CD8+ Cytotoxic T
... using live attenuated herpes viruses or entire herpes proteins have been unsuccessful because they produce pathogenic immune responses and/or fail to induce strong protective CD8+ T cell responses. T cell-mediated immune responses are believed to play an important role in controlling herpes infectio ...
... using live attenuated herpes viruses or entire herpes proteins have been unsuccessful because they produce pathogenic immune responses and/or fail to induce strong protective CD8+ T cell responses. T cell-mediated immune responses are believed to play an important role in controlling herpes infectio ...
Trafficking of Proteins to Membranes
... Trafficking of Proteins to Membranes 1. Protein fate is determined by N-terminal signal sequences 15-30 amino acids long. All contain ~10 hydrophobic residues. 2. When 70-80 amino acids have been polymerised during translation, and the signal sequence has emerged into the cytosol, it is recognised b ...
... Trafficking of Proteins to Membranes 1. Protein fate is determined by N-terminal signal sequences 15-30 amino acids long. All contain ~10 hydrophobic residues. 2. When 70-80 amino acids have been polymerised during translation, and the signal sequence has emerged into the cytosol, it is recognised b ...
Conclusion: a) The nuclear localization signal (NLS)
... 2) Nuclear export signal (NES): is in the proteins that associate with the mRNA. The details of such signals are not clear because several different sequences have been shown to serve as NES in different proteins—more diverse than the NLS. 3) The receptors: exportins The receptors for the NES sequen ...
... 2) Nuclear export signal (NES): is in the proteins that associate with the mRNA. The details of such signals are not clear because several different sequences have been shown to serve as NES in different proteins—more diverse than the NLS. 3) The receptors: exportins The receptors for the NES sequen ...
Ubiquitin Found to Mark Pathogen-Containing Vacuoles
... Dr. Coers and his colleagues wanted to figure out how the GBPs know which membrane-bound structures to go after. The researchers first conducted a large-scale screen for proteins involved in the clearance of pathogens. To their surprise, their search turned up a few proteins that play a role in ubiq ...
... Dr. Coers and his colleagues wanted to figure out how the GBPs know which membrane-bound structures to go after. The researchers first conducted a large-scale screen for proteins involved in the clearance of pathogens. To their surprise, their search turned up a few proteins that play a role in ubiq ...
Unfolded Protein Response (UPR)
... Isolation of yeast mutant which can not induce expression of BiP mRNA against misfolded protein accumulation in the ER. ...
... Isolation of yeast mutant which can not induce expression of BiP mRNA against misfolded protein accumulation in the ER. ...
Presentation Slides
... Works in presence of PEX1-G843D protein, Does not work in PEX1 null cells, PEX6 null cells, or PEX 12 cells with a missense mutation Indicates some specificity, as expected with a pharmacologic chaperone ...
... Works in presence of PEX1-G843D protein, Does not work in PEX1 null cells, PEX6 null cells, or PEX 12 cells with a missense mutation Indicates some specificity, as expected with a pharmacologic chaperone ...
Processing and Presentation
... proteasomes are introduced into the rough endoplasmic reticulum through an ATP dependent process mediated by TAP A chaperone, calnexin, associates with alpha chain. The asspciation induces conformational change that allows microglobulin to form quaternary complex. Two additional proteins, calreticul ...
... proteasomes are introduced into the rough endoplasmic reticulum through an ATP dependent process mediated by TAP A chaperone, calnexin, associates with alpha chain. The asspciation induces conformational change that allows microglobulin to form quaternary complex. Two additional proteins, calreticul ...
Use of molecular docking to highlight the mechanism of activators
... Dottorato di Ricerca in Chimica del Farmaco (XIII Ciclo) Farnesyl protein transferase (FTase) catalyzes the transfer of a farnesyl group from farnesyl diphosphate (FPP) to a specific cysteine residue of a substrate protein through covalent attachment1,2. This enzyme, like as geranylgeranyl-transfera ...
... Dottorato di Ricerca in Chimica del Farmaco (XIII Ciclo) Farnesyl protein transferase (FTase) catalyzes the transfer of a farnesyl group from farnesyl diphosphate (FPP) to a specific cysteine residue of a substrate protein through covalent attachment1,2. This enzyme, like as geranylgeranyl-transfera ...
Proteasome
Proteasomes are protein complexes inside all eukaryotes and archaea, and in some bacteria. The main function of the proteasome is to degrade unneeded or damaged proteins by proteolysis, a chemical reaction that breaks peptide bonds.