The Plant Cell - Department of Biology
... binding properties using a bacterially expressed protein. The full-length KIC cDNA sequence was inserted into pET32a vector, which expresses the KIC protein as an S-tag fusion. The fusion protein is predicted to produce a polypeptide of 33 kD. The expressed proteins were isolated and a polypeptide o ...
... binding properties using a bacterially expressed protein. The full-length KIC cDNA sequence was inserted into pET32a vector, which expresses the KIC protein as an S-tag fusion. The fusion protein is predicted to produce a polypeptide of 33 kD. The expressed proteins were isolated and a polypeptide o ...
Early events in protein folding
... polypeptide chain searches out its final native conformation from an inconceivably large number of available conformations. A polypeptide chain of 101 amino acid residues would have to sample 3100 = 5 × 1047 conformations, if each bond connecting two consecutive residues has only three possible conf ...
... polypeptide chain searches out its final native conformation from an inconceivably large number of available conformations. A polypeptide chain of 101 amino acid residues would have to sample 3100 = 5 × 1047 conformations, if each bond connecting two consecutive residues has only three possible conf ...
Ribosomal Stalk Protein L12: Structure, Function and
... ribosome i.e. mRNA decoding and peptide bond formation are driven by rRNA. In each ribosomal subunit rRNA makes the back bone or the core to which the r-proteins attach. The r-proteins often have distinct functions, yet in general, they play an important role in maintenance of the structural integri ...
... ribosome i.e. mRNA decoding and peptide bond formation are driven by rRNA. In each ribosomal subunit rRNA makes the back bone or the core to which the r-proteins attach. The r-proteins often have distinct functions, yet in general, they play an important role in maintenance of the structural integri ...
Endoplasmic reticulum localization of the low density lipoprotein
... growth factor homology domain, transmembrane domain, and cytoplasmic tail would be unnecessary for such a protein to mediate apoB degradation. To create this ER-localized LDLR, we used a truncated form of the human LDLR that binds to LDL in a Ca2⫹-dependent manner (10), analogous to the full-length ...
... growth factor homology domain, transmembrane domain, and cytoplasmic tail would be unnecessary for such a protein to mediate apoB degradation. To create this ER-localized LDLR, we used a truncated form of the human LDLR that binds to LDL in a Ca2⫹-dependent manner (10), analogous to the full-length ...
Regulation of axon growth by the JIP1–AKT axis
... neurons with the proteasome inhibitor MG132 rescued both JIP1 and AKT protein levels in axon tips, indicating that activation of glutamate neurotransmission can regulate the stability of these proteins (Fig. 5A–C). It has previously been reported that activation of kainate glutamate receptors in an ...
... neurons with the proteasome inhibitor MG132 rescued both JIP1 and AKT protein levels in axon tips, indicating that activation of glutamate neurotransmission can regulate the stability of these proteins (Fig. 5A–C). It has previously been reported that activation of kainate glutamate receptors in an ...
This article was published in an Elsevier journal. The attached copy
... often show a broader specificity, accepting both 6-oxo and 6aminopurine nucleosides. However, there are some exceptions to this type of sorting because some enzymes show subunit compositions other than trimeric or hexameric [2]. Based on the present knowledge, we can only say that mammalian and yeas ...
... often show a broader specificity, accepting both 6-oxo and 6aminopurine nucleosides. However, there are some exceptions to this type of sorting because some enzymes show subunit compositions other than trimeric or hexameric [2]. Based on the present knowledge, we can only say that mammalian and yeas ...
Acyl-CoA oxidase is imported as a heteropentameric, cofactor
... with antibodies to peroxisomal matrix (ICL, THI, and MLS) and membrane (Pex2p) proteins. Numbers and arrows indicate peak fractions for peroxisomes (P), plasma membrane (PM), mitochondria (M), endoplasmic reticulum (ER), Golgi region (G), and vacuoles (V). (D) Doublelabeling, indirect immunofluoresc ...
... with antibodies to peroxisomal matrix (ICL, THI, and MLS) and membrane (Pex2p) proteins. Numbers and arrows indicate peak fractions for peroxisomes (P), plasma membrane (PM), mitochondria (M), endoplasmic reticulum (ER), Golgi region (G), and vacuoles (V). (D) Doublelabeling, indirect immunofluoresc ...
The contribution of the Trp/Met/Phe residues to physical interactions
... physically and biochemically [25], as a critical part of the DNA damage response [26]. The physical binding of p53 TA1 domain to CBP C-terminus promotes acetylation of the p53 Cterminal lysine residues by CBP/p300. The acetylations of the lysine residues contribute to the p53 stabilization by disabl ...
... physically and biochemically [25], as a critical part of the DNA damage response [26]. The physical binding of p53 TA1 domain to CBP C-terminus promotes acetylation of the p53 Cterminal lysine residues by CBP/p300. The acetylations of the lysine residues contribute to the p53 stabilization by disabl ...
Ph.D. Thesis Azar Shahpiri
... acid (GA) signal by synthesizing hydrolytic enzymes that are released to the endosperm, before undergoing cell death. The barley (Hordeum vulgare) aleurone layer can be separated from the other seed tissues and maintained in culture, allowing the study of GA, abscisic acid (ABA) and other signals in ...
... acid (GA) signal by synthesizing hydrolytic enzymes that are released to the endosperm, before undergoing cell death. The barley (Hordeum vulgare) aleurone layer can be separated from the other seed tissues and maintained in culture, allowing the study of GA, abscisic acid (ABA) and other signals in ...
Effects of macromolecular crowding on protein folding and
... chains of similar molecular weight (dextran 70), this observation suggests that additional factors besides size exclusion effects play a role in determining protein refolding yields. The exact nature of any interactions of lysozyme with BSA and ovalbumin is unknown, but the fact that hen lysozyme is ...
... chains of similar molecular weight (dextran 70), this observation suggests that additional factors besides size exclusion effects play a role in determining protein refolding yields. The exact nature of any interactions of lysozyme with BSA and ovalbumin is unknown, but the fact that hen lysozyme is ...
Protein Interaction Technical Handbook
... interactions of proteins in a given cellular proteome (now often referred to as the “interactome”) will be the next milestone along the road to understanding the biochemistry of the cell. The ~30,000 genes of the human genome are speculated to give rise to 1 x 106 proteins through a series of post-t ...
... interactions of proteins in a given cellular proteome (now often referred to as the “interactome”) will be the next milestone along the road to understanding the biochemistry of the cell. The ~30,000 genes of the human genome are speculated to give rise to 1 x 106 proteins through a series of post-t ...
The Platform Protein Is Essential for Type IV Pilus
... Type IV pili (T4P)5 are long helical fibers composed of pilin subunits and are expressed on the surfaces of a wide variety of Gram-positive, Gram-negative, and archaeal species (1, 2). T4P assembly systems are evolutionarily and functionally related to the type II secretion (T2S) system, which is re ...
... Type IV pili (T4P)5 are long helical fibers composed of pilin subunits and are expressed on the surfaces of a wide variety of Gram-positive, Gram-negative, and archaeal species (1, 2). T4P assembly systems are evolutionarily and functionally related to the type II secretion (T2S) system, which is re ...
Identification of proteins localized to the contractile vacuole of
... V-H+-ATPase subunit a (100 kDa Subunit) Subunit a of V-ATPase (96~116 kDa protein) has been cloned from rat brain (Perin et al. 1991), human (Li et al. 1996), yeast (Manolson et al. 1992), and Dictyostelium (Liu and Clarke 1996) . It has an N-terminal hydrophilic domain exposed to the cytoplasm and ...
... V-H+-ATPase subunit a (100 kDa Subunit) Subunit a of V-ATPase (96~116 kDa protein) has been cloned from rat brain (Perin et al. 1991), human (Li et al. 1996), yeast (Manolson et al. 1992), and Dictyostelium (Liu and Clarke 1996) . It has an N-terminal hydrophilic domain exposed to the cytoplasm and ...
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... flanking NH2- and COOH-terminal sequences show more divergence. Mammals have three PP1 genes, encoding the isoforms PP1␣, PP1␥, and PP1/␦. Two splice variants can be generated from the PP1␥ gene, PP1␥1 and PP1␥2. With the exception of the testis-enriched PP1␥2, the mammalian isoforms are ubiquitous ...
... flanking NH2- and COOH-terminal sequences show more divergence. Mammals have three PP1 genes, encoding the isoforms PP1␣, PP1␥, and PP1/␦. Two splice variants can be generated from the PP1␥ gene, PP1␥1 and PP1␥2. With the exception of the testis-enriched PP1␥2, the mammalian isoforms are ubiquitous ...
Complex Degradation Processes Lead to Non
... framework, into which one can incorporate additional molecular details about specific degradation mechanisms. Experimentally, the decay patterns are often determined by the measurement of the decaying average amount of each mRNA species at steady state expression, in a cell culture after the interru ...
... framework, into which one can incorporate additional molecular details about specific degradation mechanisms. Experimentally, the decay patterns are often determined by the measurement of the decaying average amount of each mRNA species at steady state expression, in a cell culture after the interru ...
Presequence-Independent Manner Transported by the TIM23
... imidazole, 1 mM PMSF, 50 mM NaH2PO4), and bound proteins were eluted using the same buffer supplemented with 300 mM imidazole. Subsequently, imidazole was diluted using buffer C, and samples were incubated with IgG-Sepharose beads. Beads were washed extensively with buffer C, and bound protein was r ...
... imidazole, 1 mM PMSF, 50 mM NaH2PO4), and bound proteins were eluted using the same buffer supplemented with 300 mM imidazole. Subsequently, imidazole was diluted using buffer C, and samples were incubated with IgG-Sepharose beads. Beads were washed extensively with buffer C, and bound protein was r ...
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... become Pfr and translocate into the nucleus, where phys regulate the transcription of a number of genes that mediate plant light responses [12,13]. Interestingly, phy proteins form granular structures called photobodies (originally called nuclear speckles) in the nucleus in response to light. Althou ...
... become Pfr and translocate into the nucleus, where phys regulate the transcription of a number of genes that mediate plant light responses [12,13]. Interestingly, phy proteins form granular structures called photobodies (originally called nuclear speckles) in the nucleus in response to light. Althou ...
Resistance of Helicoverpa armigera to Cry1Ac toxin from Bacillus
... insect larval population processed the protein to the biologically active 65 kDa core protein, while the resistant insect larval population yielded a mixture of 95 kDa and 68 kDa Cry1Ac polypeptides. N-terminal sequencing of these 95 and 68 kDa polypeptides produced by gut juices of resistant insect ...
... insect larval population processed the protein to the biologically active 65 kDa core protein, while the resistant insect larval population yielded a mixture of 95 kDa and 68 kDa Cry1Ac polypeptides. N-terminal sequencing of these 95 and 68 kDa polypeptides produced by gut juices of resistant insect ...
Co-translational Targeting and Translocation of the Amino Terminus
... glycosylation of the tRNA-nascent opsins (48%). Because the addition of GTP was as efficient as supplementation with cytosol, the only significant/relevant factors removed by the desalting treatment were small energy molecules. In yeast, post-translational translocation depends on cytosolic proteins ...
... glycosylation of the tRNA-nascent opsins (48%). Because the addition of GTP was as efficient as supplementation with cytosol, the only significant/relevant factors removed by the desalting treatment were small energy molecules. In yeast, post-translational translocation depends on cytosolic proteins ...
Translocation of Structural P Proteins in the Phloem
... Nelson and Van Bel, 1998). However, little is known about the mechanisms or effects of translocating the numerous proteins that are known to be expressed specifically within the phloem tissue. The phloem of most angiosperms contains proteinaceous structures, collectively called P proteins (phloem pr ...
... Nelson and Van Bel, 1998). However, little is known about the mechanisms or effects of translocating the numerous proteins that are known to be expressed specifically within the phloem tissue. The phloem of most angiosperms contains proteinaceous structures, collectively called P proteins (phloem pr ...
BAG1, a negative regulator of Hsp70 chaperone activity, uncouples
... was incubated with either Hsp70, or Hsp70 and BAG-1 in the presence or absence of ATP. In the absence of ATP, Hsp70 forms a stable complex with [125I]RCMLA, as detected in the native gel assay (Figure 2, lane 2), that rapidly dissociates upon addition of ATP (Figure 2, lanes 4–9). In striking contra ...
... was incubated with either Hsp70, or Hsp70 and BAG-1 in the presence or absence of ATP. In the absence of ATP, Hsp70 forms a stable complex with [125I]RCMLA, as detected in the native gel assay (Figure 2, lane 2), that rapidly dissociates upon addition of ATP (Figure 2, lanes 4–9). In striking contra ...
The Copper Efflux Regulator CueR Is Subject to ATP
... pool to the cellular demand. In E. coli five different ATPdependent proteases (AAA+ proteases, ATPases associated with a variety of cellular activities), namely ClpXP, ClpAP, HslUV, Lon, and FtsH, are responsible for quality control of proteins as well as for the regulated turnover of intact protein ...
... pool to the cellular demand. In E. coli five different ATPdependent proteases (AAA+ proteases, ATPases associated with a variety of cellular activities), namely ClpXP, ClpAP, HslUV, Lon, and FtsH, are responsible for quality control of proteins as well as for the regulated turnover of intact protein ...
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... (c) digestion of bulk cytoplasm expressly to replenish amino acids and energy during starvation or growth factor withdrawal; (d) acting on or in concert with the molecular machineries and organelles at the interface between cell survival and cell death (e) controlling and acting as an effector or a ...
... (c) digestion of bulk cytoplasm expressly to replenish amino acids and energy during starvation or growth factor withdrawal; (d) acting on or in concert with the molecular machineries and organelles at the interface between cell survival and cell death (e) controlling and acting as an effector or a ...
Purification and Properties of the Dictyostelium Calpain
... motility, we have investigated whether a calpain-like protein is expressed in this organism. Contig 13130 (Sanger Institute Dictyostelium sequencing project) was identified as a three-exon gene that encodes a calpain-like protein. Using a custom peptide antibody to assay for the presence of this put ...
... motility, we have investigated whether a calpain-like protein is expressed in this organism. Contig 13130 (Sanger Institute Dictyostelium sequencing project) was identified as a three-exon gene that encodes a calpain-like protein. Using a custom peptide antibody to assay for the presence of this put ...
Structure and Function Relationships between ATPase Family, AAA
... Faithful replication of a cell’s genetic material during replication is essential in order to pass genetic material from one generation to the next; however, DNA-damaging agents constantly injure the genome. High fidelity replication of the cell’s genetic material is normally carried out through the ...
... Faithful replication of a cell’s genetic material during replication is essential in order to pass genetic material from one generation to the next; however, DNA-damaging agents constantly injure the genome. High fidelity replication of the cell’s genetic material is normally carried out through the ...
Proteasome
Proteasomes are protein complexes inside all eukaryotes and archaea, and in some bacteria. The main function of the proteasome is to degrade unneeded or damaged proteins by proteolysis, a chemical reaction that breaks peptide bonds.