Synthetic membrane transporters J Middleton Boon and Bradley D
... tubular antiparallel β-sheet structures in lipid bilayers. ...
... tubular antiparallel β-sheet structures in lipid bilayers. ...
Preparation of right-side-out plasma membrane
... (w/v) trichloroacetic acid (TCA). Latent activity was determined as shown in the enzyme latency section below. 5'-Nucleotidase is an accepted plasma membrane marker in animal cells which has also been used in fungi (Scarborough, 1975). The hydrolysis of AMP with resultant liberation of inorganic pho ...
... (w/v) trichloroacetic acid (TCA). Latent activity was determined as shown in the enzyme latency section below. 5'-Nucleotidase is an accepted plasma membrane marker in animal cells which has also been used in fungi (Scarborough, 1975). The hydrolysis of AMP with resultant liberation of inorganic pho ...
Topology and Phosphorylation of Soybean Nodulin
... were transcribed in vitro after linking them to bacteriophage T3 promoter. In vitro translation of these transcripts in a rabbit reticulocyte lysate, in the presence or absence of canine pancreatic microsomal membranes, suggested that nodulin-26 is cotranslationally inserted into the microsomes with ...
... were transcribed in vitro after linking them to bacteriophage T3 promoter. In vitro translation of these transcripts in a rabbit reticulocyte lysate, in the presence or absence of canine pancreatic microsomal membranes, suggested that nodulin-26 is cotranslationally inserted into the microsomes with ...
Evolution of the Bacterial Flagellum
... channels. We surmise that MotAB arose as a simple channel complex, allowing gated proton flow across the bacterial membrane, thereby dissipating H⫹ and stabilizing the cytoplasmic pH. Once these channels existed, they could have been recruited for other functions such as motility and outer membrane ...
... channels. We surmise that MotAB arose as a simple channel complex, allowing gated proton flow across the bacterial membrane, thereby dissipating H⫹ and stabilizing the cytoplasmic pH. Once these channels existed, they could have been recruited for other functions such as motility and outer membrane ...
Labeling Proteins with Small Molecules by Site
... order to be generally useful. Here we report such a method in which target proteins are expressed as fusions to a peptide carrier protein (PCP) excised from a nonribosomal peptide synthetase (NRPS). The Sfp phosphopantetheinyl transferase was used to label PCP site-specifically with small molecule-p ...
... order to be generally useful. Here we report such a method in which target proteins are expressed as fusions to a peptide carrier protein (PCP) excised from a nonribosomal peptide synthetase (NRPS). The Sfp phosphopantetheinyl transferase was used to label PCP site-specifically with small molecule-p ...
Batesian Mimicry: Can a Leopard Change Its Spots — and Get
... Figure 1. Batesian mimicry shifts across a faunal suture zone in Peru. Mimicry represents a unique opportunity to study variation in the degree of local adaptation, since the warning signals used by local model species represent known fitness optima for mimics. Forest butterfly communities in Amazon ...
... Figure 1. Batesian mimicry shifts across a faunal suture zone in Peru. Mimicry represents a unique opportunity to study variation in the degree of local adaptation, since the warning signals used by local model species represent known fitness optima for mimics. Forest butterfly communities in Amazon ...
- Wiley Online Library
... steps occurring in the plant. In this same paper, the localization of a GUS^VirE2 fusion was investigated. The fusion protein localized in the nucleus of plant cells and this localization was dependent on the presence of NLSs (nuclear localization signals) on VirE2. These results are in apparent con ...
... steps occurring in the plant. In this same paper, the localization of a GUS^VirE2 fusion was investigated. The fusion protein localized in the nucleus of plant cells and this localization was dependent on the presence of NLSs (nuclear localization signals) on VirE2. These results are in apparent con ...
Phospholipids are amphipathic molecules that make up
... The cell membrane consists of two adjacent layers of phospholipids, which form a bilayer. The fatty acid tails of phospholipids face inside, away from water, whereas the phosphate heads face the outward aqueous side. Since the heads face outward, one layer is exposed to the interior of the cell and ...
... The cell membrane consists of two adjacent layers of phospholipids, which form a bilayer. The fatty acid tails of phospholipids face inside, away from water, whereas the phosphate heads face the outward aqueous side. Since the heads face outward, one layer is exposed to the interior of the cell and ...
STRUCTURE AND FUNCTION OF THE CHLOROPLAST Ndh
... that binds NADH. Several experiments and the probable analogy with the respiratory complex I suggest the probable orientation in thylakoid of the Ndh complex shown bellow. The nuclear genes that probably encode the subunits NQO1, NQO2 y NQO3 (in white) still remain to be identified. In contrast, the ...
... that binds NADH. Several experiments and the probable analogy with the respiratory complex I suggest the probable orientation in thylakoid of the Ndh complex shown bellow. The nuclear genes that probably encode the subunits NQO1, NQO2 y NQO3 (in white) still remain to be identified. In contrast, the ...
ATP-driven Pumps
... In addition there is a change in binding site affinity: allows ion pick-up at low concn on one side allows ion release at high concn on other side These are the two ways in which free energy from ATP hydrolysis is expended. ...
... In addition there is a change in binding site affinity: allows ion pick-up at low concn on one side allows ion release at high concn on other side These are the two ways in which free energy from ATP hydrolysis is expended. ...
FYVE-dependent endosomal targeting of an arrestin-related
... signaling and their trafficking. The arrestin superfamilly includes several arrestin domain-containing proteins and the structurally related protein Vps26. In Dictyostelium discoideum, the arrestin-domain containing proteins form a family of six members, namely AdcA to -F. In contrast to canonical a ...
... signaling and their trafficking. The arrestin superfamilly includes several arrestin domain-containing proteins and the structurally related protein Vps26. In Dictyostelium discoideum, the arrestin-domain containing proteins form a family of six members, namely AdcA to -F. In contrast to canonical a ...
SuperSignal Western Blot Enhancer
... Tween® is a registered trademark of ICI Americas. This product (“Product”) is warranted to operate or perform substantially in conformance with published Product specifications in effect at the time of sale, as set forth in the Product documentation, specifications and/or accompanying package insert ...
... Tween® is a registered trademark of ICI Americas. This product (“Product”) is warranted to operate or perform substantially in conformance with published Product specifications in effect at the time of sale, as set forth in the Product documentation, specifications and/or accompanying package insert ...
Role of cholesterol in SNARE-mediated trafficking on intracellular
... recycling endosomes (RE) by either endocytosis of LDL through clathrin coated pits (ccp), or selective cholesteryl ester (CE) uptake by SRB1 from HDL in cholesterol-rich (chol-rich, e.g. caveolae) plasma membrane domains (shown in dark gray). (2) LDL is then transported to late endosomes/multivesicu ...
... recycling endosomes (RE) by either endocytosis of LDL through clathrin coated pits (ccp), or selective cholesteryl ester (CE) uptake by SRB1 from HDL in cholesterol-rich (chol-rich, e.g. caveolae) plasma membrane domains (shown in dark gray). (2) LDL is then transported to late endosomes/multivesicu ...
The Plasma Membrane
... In this form of pinocytosis, called clathrin-mediated endocytosis, cell-surface receptors bind to individual molecules of the substance to be taken into the cell and then move laterally across the plasma membrane to a pit, coated on its underside with the protein clathrin, that will become a vesicle ...
... In this form of pinocytosis, called clathrin-mediated endocytosis, cell-surface receptors bind to individual molecules of the substance to be taken into the cell and then move laterally across the plasma membrane to a pit, coated on its underside with the protein clathrin, that will become a vesicle ...
University of Groningen AthPEX10, ariuclear gene essential
... their development, whereas the viable, green seeds developed into brown mature seeds (Fig. 1E). Analyses of two heterozygous lines yielded a frequency of 21.3% ⫾ 7.1% (SD) and 21.6% ⫾ 4.5% lethal seeds in agreement with a recessive lethal segregation of the embryos homozygous for the insertion. In a ...
... their development, whereas the viable, green seeds developed into brown mature seeds (Fig. 1E). Analyses of two heterozygous lines yielded a frequency of 21.3% ⫾ 7.1% (SD) and 21.6% ⫾ 4.5% lethal seeds in agreement with a recessive lethal segregation of the embryos homozygous for the insertion. In a ...
Antibacterial activity of zinc(II) and copper(II) terpyridine
... therapy, (ii) copper chelating agents were developed to treat Wilson disease, an autosomal recessive genetic disorder that causes copper accumulation primarily in the liver. In general, organic ligands can contribute to better transport of metal ions through the lipophillic regions of cell membranes ...
... therapy, (ii) copper chelating agents were developed to treat Wilson disease, an autosomal recessive genetic disorder that causes copper accumulation primarily in the liver. In general, organic ligands can contribute to better transport of metal ions through the lipophillic regions of cell membranes ...
Transmembrane domains control exclusion of membrane proteins
... extracellular domain of CD1b fused to the TMD of various membrane proteins. We used CD1b as a reporter protein because this protein normally traffics through endocytic compartments (Jackman et al., 1998) and thus should not be sensitive to proteases found in endosomal compartments. Indeed, we have o ...
... extracellular domain of CD1b fused to the TMD of various membrane proteins. We used CD1b as a reporter protein because this protein normally traffics through endocytic compartments (Jackman et al., 1998) and thus should not be sensitive to proteases found in endosomal compartments. Indeed, we have o ...
Membrane Trafficking During Plant Cytokinesis
... vesicle budding components that function in concert with dynamin to promote membrane scission (46). In addition to their role in vesicular trafficking, dynamin and dynamin-related proteins have been linked to a number of diverse processes including actin dynamics (47). The plant-specific, dynamin-rela ...
... vesicle budding components that function in concert with dynamin to promote membrane scission (46). In addition to their role in vesicular trafficking, dynamin and dynamin-related proteins have been linked to a number of diverse processes including actin dynamics (47). The plant-specific, dynamin-rela ...
Neurophysiology
... Synaptic cleft – fluid filled space between pre and post synaptic neuron Postsynaptic neuron - transmits impulse away from synapse, contains receptors for neurotransmitters ...
... Synaptic cleft – fluid filled space between pre and post synaptic neuron Postsynaptic neuron - transmits impulse away from synapse, contains receptors for neurotransmitters ...
Neurophysiology
... Synaptic cleft – fluid filled space between pre and post synaptic neuron Postsynaptic neuron - transmits impulse away from synapse, contains receptors for neurotransmitters ...
... Synaptic cleft – fluid filled space between pre and post synaptic neuron Postsynaptic neuron - transmits impulse away from synapse, contains receptors for neurotransmitters ...
The YidC/Oxa1/Alb3 protein family Saller, Manfred J.
... Biological membranes of living cells form hydrophobic barriers that separate the intracellular space from the extracellular environment and allow the maintenance of distinct cellular compartments. Many essential cellular processes depend on the function of membrane proteins that are anchored to the ...
... Biological membranes of living cells form hydrophobic barriers that separate the intracellular space from the extracellular environment and allow the maintenance of distinct cellular compartments. Many essential cellular processes depend on the function of membrane proteins that are anchored to the ...
Membrane Proteins - Hillsborough Community College
... matrix (fibers and other substances outside the cell) may be anchored to membrane proteins, which help maintain cell shape and fix the location of certain membrane proteins. Others play a role in cell movement or bind adjacent cells together. ...
... matrix (fibers and other substances outside the cell) may be anchored to membrane proteins, which help maintain cell shape and fix the location of certain membrane proteins. Others play a role in cell movement or bind adjacent cells together. ...
SNARE (protein)
SNARE proteins (an acronym derived from ""SNAP (Soluble NSF Attachment Protein) REceptor"") are a large protein superfamily consisting of more than 60 members in yeast and mammalian cells. The primary role of SNARE proteins is to mediate vesicle fusion, that is, the fusion of vesicles with their target membrane bound compartments (such as a lysosome). The best studied SNAREs are those that mediate docking of synaptic vesicles with the presynaptic membrane in neurons. These SNAREs are the targets of the bacterial neurotoxins responsible for botulism and tetanus.