Analysis of Protein Interactions at Native Chloroplast Membranes by
... quantified by total internal reflection ellipsometry (TIRE). We show that native chloroplast membranes deposited on goldcoated glass slides using Langmuir-Schaefer films retain functional receptors capable of binding chaperones with high specificity and affinity. Taking into account the low chaperon ...
... quantified by total internal reflection ellipsometry (TIRE). We show that native chloroplast membranes deposited on goldcoated glass slides using Langmuir-Schaefer films retain functional receptors capable of binding chaperones with high specificity and affinity. Taking into account the low chaperon ...
Mycobacterium tuberculosis evades macrophage defenses by
... envelope that prevents bacteria from escaping. This process leads to containment and killing of the bacteria and is also associated with rapid priming of antigen-specific T cell responses. In contrast, virulent mycobacteria such as wild-type strains of MTb cause macrophage death by a process that pr ...
... envelope that prevents bacteria from escaping. This process leads to containment and killing of the bacteria and is also associated with rapid priming of antigen-specific T cell responses. In contrast, virulent mycobacteria such as wild-type strains of MTb cause macrophage death by a process that pr ...
Undergraduate/Graduate Category: Interdisciplinary Topics, Centers and Institutes Degree Level:Graduate
... • Liposome composed of lipid with neutral spontaneous curvature cannot fuse spontaneously in 50mM CaCl 2 solution at room temperature • Experiments were repeated for 56 times in identical conditions, liposome fusion was found in 5 of them ...
... • Liposome composed of lipid with neutral spontaneous curvature cannot fuse spontaneously in 50mM CaCl 2 solution at room temperature • Experiments were repeated for 56 times in identical conditions, liposome fusion was found in 5 of them ...
Mechanisms of mitochondrial protein import
... Atm1p, an ATP-binding-cassette transporter) appear to follow the general Tim17-23 matrix pathway (discussed in the next section). As the N-terminus of the carrier protein becomes exposed to the IMS, it binds to a soluble 70 kDa complex consisting of Tim9 and Tim10 proteins. Such an interaction pulls ...
... Atm1p, an ATP-binding-cassette transporter) appear to follow the general Tim17-23 matrix pathway (discussed in the next section). As the N-terminus of the carrier protein becomes exposed to the IMS, it binds to a soluble 70 kDa complex consisting of Tim9 and Tim10 proteins. Such an interaction pulls ...
The Plasma Membrane of the Cyanobacterium
... evolutionary origin and the functional advantages of thylakoid membranes but also might include insights regarding thylakoid formation during chloroplast differentiation. Based on biochemical isolation and direct in vivo characterization, we report here structural and functional domains in the cytop ...
... evolutionary origin and the functional advantages of thylakoid membranes but also might include insights regarding thylakoid formation during chloroplast differentiation. Based on biochemical isolation and direct in vivo characterization, we report here structural and functional domains in the cytop ...
Protein translocation across mitochondrial membranes
... against a synthetic mitochondrial presequence(*’). Surprisingly the primary sequence of P32 was found to be identical to that of the yeast phosphate carrier(30). Immunoprecipitation from digitonin extracts of mitochondria using antibodies against MOM19 and MOM72 led to the discovery of a receptor co ...
... against a synthetic mitochondrial presequence(*’). Surprisingly the primary sequence of P32 was found to be identical to that of the yeast phosphate carrier(30). Immunoprecipitation from digitonin extracts of mitochondria using antibodies against MOM19 and MOM72 led to the discovery of a receptor co ...
Total Internal reflection Fluorescence Microscopy: Instrumentation
... proportional to evanescent intensity I(z). If fluorophore moves from z1 to z2, z= z1- z2 = dln (I2/I1) This relationship is valid even in those cases where there are multiple fluorophores attached to the same structure or irregularly shaped fluorophores. ...
... proportional to evanescent intensity I(z). If fluorophore moves from z1 to z2, z= z1- z2 = dln (I2/I1) This relationship is valid even in those cases where there are multiple fluorophores attached to the same structure or irregularly shaped fluorophores. ...
CK12 Passive Transport - Diffusion, Osmosis, and Facilitated Diffusion
... As was stated in the previous section, a semipermeable membrane allows small particles like oxygen and water to pass right through the cell membrane. Particles such as sugar and amino acids are too large to fit through the semipermeable cell membrane and need to find a passage way through the membra ...
... As was stated in the previous section, a semipermeable membrane allows small particles like oxygen and water to pass right through the cell membrane. Particles such as sugar and amino acids are too large to fit through the semipermeable cell membrane and need to find a passage way through the membra ...
6.5 Neurons and Synapses
... Choline reabsorbed back into pre-synaptic neuron where it is converted back into a neurotransmitter ...
... Choline reabsorbed back into pre-synaptic neuron where it is converted back into a neurotransmitter ...
How is the intracellular fate of the Legionella pneumophila
... which, if any, of the Icm/Dot proteins are effector proteins, but there are at least two candidates. DotI/IcmL contains two amphipathic -sheet regions6, structures which are found in pore-forming toxins. The pore-forming ability, which was shown to be associated with the Icm/Dot proteins7, might be ...
... which, if any, of the Icm/Dot proteins are effector proteins, but there are at least two candidates. DotI/IcmL contains two amphipathic -sheet regions6, structures which are found in pore-forming toxins. The pore-forming ability, which was shown to be associated with the Icm/Dot proteins7, might be ...
Triton X-100 promotes a cholesterol
... We analysed the lipid structure of the cell surface of COS cells by means of two-photon microscopy. The fluorescent probe Laurdan has been used to characterize phase separation in model membranes [24,25] and visualize ordered domains on the surface of living cells [21]. Laurdan does not partition pr ...
... We analysed the lipid structure of the cell surface of COS cells by means of two-photon microscopy. The fluorescent probe Laurdan has been used to characterize phase separation in model membranes [24,25] and visualize ordered domains on the surface of living cells [21]. Laurdan does not partition pr ...
Case Study: Membranes - Theoretical and Computational
... monly found types of synthetic phospholipids lipid bilayers. in laboratory experiments include dipalmitoylglycero-phosphocholine (DPPC), 1-palmitoyl2-oleoyl-glycerol-phosphocholne (POPC), and 1-palmitoyl-2-oleoyl-glycerophosphoethanolamine (POPE). We will use VMD to look at the structures of these l ...
... monly found types of synthetic phospholipids lipid bilayers. in laboratory experiments include dipalmitoylglycero-phosphocholine (DPPC), 1-palmitoyl2-oleoyl-glycerol-phosphocholne (POPC), and 1-palmitoyl-2-oleoyl-glycerophosphoethanolamine (POPE). We will use VMD to look at the structures of these l ...
Lipid Domain Theory of General Anesthesia - Deep Blue
... plasma membranes of intact cells are unable to fully phase separate but rather form structures on the length-scale of the cortical meshwork that may have significance in membrane component transport across the membrane. Because one of our goals is to observe phase separation in membrane, interferenc ...
... plasma membranes of intact cells are unable to fully phase separate but rather form structures on the length-scale of the cortical meshwork that may have significance in membrane component transport across the membrane. Because one of our goals is to observe phase separation in membrane, interferenc ...
A major glycoprotein of the nuclear pore complex is a membrane
... proteins of the pore complex have been identified in higher eucaryotes by imunocytochemistry (for review, see Gerace and Burke, 1988). The first of these was a glycoprotein called gpl90 that was identified in rat liver nuclear envelopes with polyclonal antibodies (Gerace et al., 1982). This protein ...
... proteins of the pore complex have been identified in higher eucaryotes by imunocytochemistry (for review, see Gerace and Burke, 1988). The first of these was a glycoprotein called gpl90 that was identified in rat liver nuclear envelopes with polyclonal antibodies (Gerace et al., 1982). This protein ...
Membrane Protein Sorting in the Yeast Secretory Pathway
... punctate structures per cell that exhibit a somewhat random distribution within the cytoplasmic compartment (Franzusoff et al., 1991; Redding et al., 1991). Thus, the Golgi apparatus of yeast is not localized to a perinuclear location, as in mammalian cells, but rather is dispersed throughout the ce ...
... punctate structures per cell that exhibit a somewhat random distribution within the cytoplasmic compartment (Franzusoff et al., 1991; Redding et al., 1991). Thus, the Golgi apparatus of yeast is not localized to a perinuclear location, as in mammalian cells, but rather is dispersed throughout the ce ...
Imaging T-tubules: dynamic membrane
... at jSR junctions is an important emergent area of membrane physiology and disease mechanisms. Ventricular myocytes (VMs) may critically depend on the variability and integrity of thousands of TT-associated CRUs in order to sustain 3 000 000 000 contraction–relaxation cycles during a typical human l ...
... at jSR junctions is an important emergent area of membrane physiology and disease mechanisms. Ventricular myocytes (VMs) may critically depend on the variability and integrity of thousands of TT-associated CRUs in order to sustain 3 000 000 000 contraction–relaxation cycles during a typical human l ...
groupfeb08 - University of Pennsylvania
... Effect of Glycocalyx (Experimental Data) Binding of carriers increases about 4 fold upon infusion of heparinase. Glycocalyx may shield beads from binding to ICAMs ...
... Effect of Glycocalyx (Experimental Data) Binding of carriers increases about 4 fold upon infusion of heparinase. Glycocalyx may shield beads from binding to ICAMs ...
The munc13-4–rab27 complex is specifically
... munc13-4 is an unusual rab27 effector in this pathway because it lacks a conserved rab27-binding domain (R27BD), does not need rab27 interaction for membrane association, and is the only known essential rab27 effector in cytotoxic lymphocytes. Rab27 binding requires aa 240-543,10,21 but more precise ...
... munc13-4 is an unusual rab27 effector in this pathway because it lacks a conserved rab27-binding domain (R27BD), does not need rab27 interaction for membrane association, and is the only known essential rab27 effector in cytotoxic lymphocytes. Rab27 binding requires aa 240-543,10,21 but more precise ...
1. Small Scale Expression of Tagged Recombinant Proteins
... 1. Thaw the required number of tubes of cells on ice and mix gently to ensure that the cells are evenly suspended. Transform also with empty vector. 2. Add 1 μl of the DNA solution directly to the cells. Stir gently to mix. 3. Place the tubes on ice for 5 min. 4. Heat the tubes for exactly 30 s in a ...
... 1. Thaw the required number of tubes of cells on ice and mix gently to ensure that the cells are evenly suspended. Transform also with empty vector. 2. Add 1 μl of the DNA solution directly to the cells. Stir gently to mix. 3. Place the tubes on ice for 5 min. 4. Heat the tubes for exactly 30 s in a ...
Plant Nutrition: Root Transporters on the Move
... transport of nutrients and water toward the vascular tissues is achieved by a battery of specialized transporters and channels. Modulating the amount and the localization of these membrane transport proteins appears as a way to drive their activity and is essential to maintain nutrient homeostasis i ...
... transport of nutrients and water toward the vascular tissues is achieved by a battery of specialized transporters and channels. Modulating the amount and the localization of these membrane transport proteins appears as a way to drive their activity and is essential to maintain nutrient homeostasis i ...
4-6
... membrane can vary considerably and is a function of membrane thickness and pore size. The water permeability is indicated by its ultrafiltration coefficient (KUf). The KUf is defined as the number of milliliters of fluid per hour that will be transferred across the membrane per mm Hg pressure gradie ...
... membrane can vary considerably and is a function of membrane thickness and pore size. The water permeability is indicated by its ultrafiltration coefficient (KUf). The KUf is defined as the number of milliliters of fluid per hour that will be transferred across the membrane per mm Hg pressure gradie ...
SNARE (protein)
SNARE proteins (an acronym derived from ""SNAP (Soluble NSF Attachment Protein) REceptor"") are a large protein superfamily consisting of more than 60 members in yeast and mammalian cells. The primary role of SNARE proteins is to mediate vesicle fusion, that is, the fusion of vesicles with their target membrane bound compartments (such as a lysosome). The best studied SNAREs are those that mediate docking of synaptic vesicles with the presynaptic membrane in neurons. These SNAREs are the targets of the bacterial neurotoxins responsible for botulism and tetanus.