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Review Questions
Review Questions

... cytoplasm? DNA is transcribed into messenger RNA. 2. What is a transcript? A transcript is not a copy of the original but has the same information but stored in a different form. Court reporters make a transcript of courtroom proceedings. They type out what everyone says during a trial. Rather than ...
Chapter 13
Chapter 13

... In the absence of tryptophan, the ribosome stalls at the tryptophan codons and an alternative secondary structure prevents formation of the hairpin, so that transcription continues. ...
RNA and Protein Synthesis
RNA and Protein Synthesis

... sex cells, contains a complete copy of your DNA. Why, then, are some cells nerve cells with dendrites and axons, while others are red blood cells that have lost their nuclei and are packed with hemoglobin? Why are cells so different in structure and function? If the characteristics of a cell depend ...
From Gene to Protein
From Gene to Protein

...   They protect mRNA from hydrolytic enzymes.   They help ribosomes attach to the 5ʹ′ end. ...
LOCALIZATION OF A MOLECULE
LOCALIZATION OF A MOLECULE

... Methods for localization • PROTEIN Immunolocalization endogenous or tagged? Direct detection via gfp or enzyme (b-gal) Gold Particle Cell fractionation • RNA In Situ Hybridization in cells, tissues, embryos • DNA FISH ...
Dicer-Like
Dicer-Like

... What is Dicer’s role in RNAi? • Activated by exogenous double-stranded (ds) RNA • miRNA (micro RNA) -small, non-coding regions of double-stranded (ds) RNA 21-22 nucleotides ...
Chapter 13
Chapter 13

... 2. It is made of monomers called nucleotides 3. There are two differences between a DNA & an RNA nucleotide: - RNA has ribose instead of deoxyribose - RNA has the base Uracil instead of ...
Gene_expression
Gene_expression

... make the mRNA able to create proteins. • Cells do this by spliceosome's binding on either side of an intron, looping the intron into a circle and then cleaving it off. • The two ends of the exons are then joined ...
Messenger RNA profiling: a prototype method to supplant
Messenger RNA profiling: a prototype method to supplant

... resembling mRNA structure but located in DNA Control: amplify DNA, look for ...
Journal
Journal

... Given the amino acid sequence histidine-tryptophan-leucinelysine-proline: What is the sequence of bases coding for this ...
Unit 5 : Cell Growth and Reproduction Lesson 1: Genes and
Unit 5 : Cell Growth and Reproduction Lesson 1: Genes and

... • Ribosomes are either ___________________________ or are free floating in the cytoplasm. • If the protein is being made for the plasma membrane they will be made on the rER. • If protein is being made for the cell to use they will be made on free ribosomes. • The protein made on rER will be packed ...
Translation
Translation

... • for each C encountered on the DNA strand, a G is inserted in the RNA • for each G, a C • for each T, an A • however, each A on the DNA guides the insertion of the pyrimidine uracil, U instead of A ...
Semester Test Practice Test
Semester Test Practice Test

... • b. it is difficult to destroy in the lab • c. it is single-celled ...
Document
Document

... 3’ end of the transcript typically contains AAUAAA or AUUAAA. This sequence is recognized by an enzyme that cleaves the newly synthesized transcript ~20 nucleotides downstream. ...
Key
Key

... 15. For each of the following sequences, indicate if it is made of DNA, RNA or amino acids and then name the protein or complex that will bind to it. ...
Assignment 1 solution
Assignment 1 solution

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Week 2 - DQ`s
Week 2 - DQ`s

... chromosomes of cells undergoing mitosis will be visible. How does colchicine achieve this effect? What is the natural source of colchicines. ...
P-RNA (Phyto-Ribonucleic Acid) What is RNA? Why do we need it
P-RNA (Phyto-Ribonucleic Acid) What is RNA? Why do we need it

... to link up with. This is where transfer RNA comes in. 2) Transfer RNA seeks out and brings each individual amino acid to the chain and parks it in its particular slot where it will do the most good and be the most beneficial. Now enters rRNA (ribosomal RNA). 3) Ribosomal RNA provides the machinery o ...
MS Word
MS Word

... Capsid forms and budding forms bullet envelope Zoonotic infection-from bite of animal (or other close association) Canine problem throughout the world-no vaccination of dogs In US skunks, coyotes, and raccoons are becoming bigger problem Last 12 fatalities in US associated with bats (no bites eviden ...
What is RNA splicing?
What is RNA splicing?

... Genetic information is transferred from genes to the proteins they encode via a “messenger” RNA ...
Understanding Genetic Control Elements
Understanding Genetic Control Elements

... serving as a binding site for specific proteins. Enhancers may be physically separated from the promoter, but still influence it. Duplicating enhancers or modifying their DNA sequences can dramatically alter activity. ...
Datasheet Blank Template - Santa Cruz Biotechnology
Datasheet Blank Template - Santa Cruz Biotechnology

... family of multifunctional proteins that regulate both transcription and translation. Y-box proteins interact with a wide variety of nucleic acid structures to act as transcription factors and mRNA masking proteins. The modular structure of Y-box proteins includes a highly conserved N-terminal coldsh ...
RNA and Protein Synthesis
RNA and Protein Synthesis

... Create a strand of mRNA ...
7.2.A1 The promoter as an example of non
7.2.A1 The promoter as an example of non

... operator. RNA polymerase binds to the _____________ allowing the genes that produce proteins involved in lactose metabolism ___________ be transcribed. ...
Protein Synthesis Quick Questions
Protein Synthesis Quick Questions

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RNA-binding protein

RNA-binding proteins (often abbreviated as RBPs) are proteins that bind to the double or single stranded RNA in cells and participate in forming ribonucleoprotein complexes.RBPs contain various structural motifs, such as RNA recognition motif (RRM), dsRNA binding domain, zinc finger and others.They are cytoplasmic and nuclear proteins. However, since most mature RNA is exported from the nucleus relatively quickly, most RBPs in the nucleus exist as complexes of protein and pre-mRNA called heterogeneous ribonucleoprotein particles (hnRNPs).RBPs have crucial roles in various cellular processes such as: cellular function, transport and localization. They especially play a major role in post- transcriptional control of RNAs, such as: splicing, polyadenylation, mRNA stabilization, mRNA localization and translation. Eukaryotic cells encode diverse RBPs, approximately 500 genes, with unique RNA-binding activity and protein-protein interaction. During evolution, the diversity of RBPs greatly increased with the increase in the number of introns. Diversity enabled eukaryotic cells to utilize RNA exons in various arrangements, giving rise to a unique RNP (ribonucleoprotein) for each RNA. Although RBPs have a crucial role in post-transcriptional regulation in gene expression, relatively few RBPs have been studied systematically.
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