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Slides
Slides

... • left = bi-partite graph for GO terms (yellow -> red scale) and miRNA (grey) • edge is enrichment of the GO term in the set of genes most correlated with the miRNA. • right = GO summary network for GO terms in the left enrichment network. Each GO nodes color-coded by cumulative enrichment score for ...
PDF
PDF

... influences many aspects of mRNA metabolism: transcription termination by RNAP II, mRNA stability, mRNA export to the cytoplasm and the efficiency of translation are all dependent on 39 processing. In recent years it has become increasingly evident that APA regulates gene expression [2]. In some case ...
Human Monoclonal Antibodies
Human Monoclonal Antibodies

... region is in blue. These are linked by an alpha helix (purple). Disulfide bonds were added at three locations in T4 lysozyme to increase the stability. The first disulfide was between positions 9 and 164. This links the first alpha helix at the N terminus with the C-terminal tail. The second disulfi ...
Gene Expression in Prokaryotes
Gene Expression in Prokaryotes

... Actvators ...
2 Pyruvic Acid
2 Pyruvic Acid

... During respiration electrons are removed from glucose and transported to the ETC by electron carriers. Energy from the electrons is used to synthesize ATP in the ETC. ...
The parasitic plant dodder (Cuscuta pentagona) establishes a
The parasitic plant dodder (Cuscuta pentagona) establishes a

... The parasitic plant dodder (Cuscuta pentagona) establishes a continuous vascular system through which water and nutrients are drawn. To discover the route of mRNA transportation, insitu RT-PCR technique was used to locally amplifiy host transcript within parasitic tissue. The stability of host mRNA ...
Nucleic acid enzymes
Nucleic acid enzymes

... observed. Unfortunately, sequence generality was seriously hampered by the minimal requirement of five specific RNA nucleotides around the ligation site and, for optimal activity, as many as eight RNA nucleotides had to be conserved. The development of 30 –50 RNA ligase DNAzymes with broad generalit ...
Human fetal normal cDNA panel
Human fetal normal cDNA panel

... MgCl2, 10mM DTT). The estimated cDNA concentration is about 5.5ng/µl. 1µl cDNA is good enough for one PCR reaction. The 5' end of human clathrin cDNA (a 6 kb gene) has been amplified by PCR from all of these cDNAs. ...
Genome Annotation
Genome Annotation

... After a good ORF is found, it is extended 5’ to find possible start codons (but only allowing 6 bases of overlap with another gene). Ribosome binding sites are then defined, based on genes that have only 1 possible start codon. Twenty bases upstream from the start sites are aligned. ...
File
File

... The three enzymes involved in the metabolism of lactose are transcribed and expressed cAMP binds to CAP regulatory protein, causing it to bind to the promoter of the lac operon The enzymes needed for lactose metabolism must be transcribed when lactose is present cAMP levels increase because glucose ...
Name __ DNA, RNA, and PROTEINS TEST (2 points each
Name __ DNA, RNA, and PROTEINS TEST (2 points each

... _____ The Hershey-Chase blender experiments and Avery’s enzyme digestion experiments helped to prove that _____________________ A. DNA is a double helix. B. pneumonia causes dead mice. C. transformation is caused by proteins D. the genetic material is made of DNA _____ The series of genes that contr ...
MICRO. 555 (555 Microbial Molecular Genetics) Dr.Afaf Ibrahim
MICRO. 555 (555 Microbial Molecular Genetics) Dr.Afaf Ibrahim

... existence of 19,599 protein-coding genes in the human genome and identified another 2,188 DNA segments that are predicted to be protein-coding genes = 21,787 genes mtDNA & Y-chromosome DNA aid in search for our human ancestry Practical Applications of DNA Technology - Some examples of What's been Do ...
DNA-binding proteins
DNA-binding proteins

... © 2015 Pearson Education, Inc. ...
View Poster - Technology Networks
View Poster - Technology Networks

... We found 68 candidate miRNA precursors Four miRNA candidates out of 8 tested by northern blot showed differential expression in gametes and vegetative cells Potential targets for four out of 18 tested candidate miRNAs could be verified by 5’ RACE analysis, showing cleavage in the centre of the predi ...
Probability in transcriptional regulation and its
Probability in transcriptional regulation and its

... range. This increase was shown to result from increased transcription initiation using a nuclear run on transcription assay.1 We concluded that LPS induces PAI-2 mRNA transcription, the implication being that each cell starts transcribing the gene more rapidly. However, using single-cell assays, we ...
Slide 1
Slide 1

... using some of the Cn3D features that allow you to: – spin the structure using your mouse – use the control+left mouse button combination to zoom in and out of the structure – use the shift+left mouse button combination to move the structure across the viewing window – use the Style menu to render th ...
Biology 2250 - Memorial University
Biology 2250 - Memorial University

... without any clear understanding of the molecular nature of the gene. The ontogeny of most courses follows this phylogeny. However, a certain pretense is required: when we talk about round and wrinkled peas, we pretend you don't know about DNA, because Mendel didn't. This approach works well through ...
Example 2. - the Gene Ontology Consortium Wiki
Example 2. - the Gene Ontology Consortium Wiki

... Given the crucial role of BMPs in osteoblastic differentiation, we tested whether CYR61 mediated alteration of osteoblast proliferation and differentiation through regulation of BMP expression. According to Cheng et al. (43), BMP-2, -6, and -9 may be the most potent molecules that induce osteoblast ...
Spotted
Spotted

... - Gene Ontology is a “controlled vocabulary that can be applied to all eukaryotes “. Each gene product is classified in one or more categories. - Is distribution of missexpressed genes significantly different from the one of our initial set of genes? - maybe ash2 acts predominantly upon a group of g ...
Lec 10 - Regulation of Gene
Lec 10 - Regulation of Gene

... In lac operon of E. coli, the promotor gene is located next to operator. This is located between operator gene and regulator gene. The promotor segment is a place where mRNA polymerase enzyme binds with DNA. The recent investigations (Pribnow, 19715) suggested that promotor segment has three sub reg ...
PowerPoint プレゼンテーション
PowerPoint プレゼンテーション

... in yellow. Note that a TALEN is considered valid if any transcript’s second exon is targeted. Targeting the second exon is likely to introduce knockout mutations in the Pten gene. ...
Slides PPT - The University of Sydney
Slides PPT - The University of Sydney

... • Let’s consider the glucocorticoid response first. • Cortisol is the active glucocorticoid hormone. • Pharmaceutical analogues are cortisone (converted to cortisol by a dehydrogenase) and the synthetic analogues prednisone and ...
A Survey of Intron Research in Genetics
A Survey of Intron Research in Genetics

... The existence of the intron-exon structure has been particularly intriguing. Introns are only found in eukaryotic genomes and make up a large portion of the DNA in eukaryotic genomes. In humans, for example, approximately 30% of the human genome is made up of introns [1]. Only about 3% consists of c ...
Biol120 Mock Final Examination (v2.0)
Biol120 Mock Final Examination (v2.0)

... 19. There are 4 alleles for a given gene and an individual is heterozygous for that gene. How many alleles for that gene will he/she possess in each of their gametes? a) 1 b) 2 c) 3 d) 4 20. What is/are the fate(s) of electrons in a pigment being raised to a higher energy level or excited state? a) ...
Supplemental Data High Coding Density on the Largest
Supplemental Data High Coding Density on the Largest

... The frequencies of dinucleotides for the megabase chromosome sequence were calculated taking into account the frequencies of each individual nucleotide and are given as the ratio of observed to expected frequencies, fobs/exp. We found both CpG and TpA dinucleotides to be underrepresented with respec ...
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