Supplementary Notes
... Figure 6a, lanes 5–23). One exception was Gly, which was not incorporated into GFP at all (lanes 4 and 24). Because it was confirmed that Gly was attached to the 3'-end of tRNA like the other amino acids, the complete lack of incorporation was unanticipated. We thus suspected that unknown mechanisms ...
... Figure 6a, lanes 5–23). One exception was Gly, which was not incorporated into GFP at all (lanes 4 and 24). Because it was confirmed that Gly was attached to the 3'-end of tRNA like the other amino acids, the complete lack of incorporation was unanticipated. We thus suspected that unknown mechanisms ...
Text Book of Molecular Biology
... then link together the two ends,the twisting formed deformation is locked into the system. This deformation is shown as supercoiling, since it manifests itself as a coiling of the DNA axis around itself in a higher-order coil, and corresponds to a change in linking number from the relaxed circular ...
... then link together the two ends,the twisting formed deformation is locked into the system. This deformation is shown as supercoiling, since it manifests itself as a coiling of the DNA axis around itself in a higher-order coil, and corresponds to a change in linking number from the relaxed circular ...
Presentation - University of Warwick
... • Can be used in the following ways: 1.To ensure superfluous parameters have minimal effect on gene expression (eg batching effects) 2.To find interesting parameters ...
... • Can be used in the following ways: 1.To ensure superfluous parameters have minimal effect on gene expression (eg batching effects) 2.To find interesting parameters ...
AutoMotif server: prediction of single residue post-translational
... the LFM. The negative instances were chosen randomly from those that do not include experimentally verified PTM of any type. These two datasets (positive and negative instances) were projected as sets of points in a multidimensional space (http://automotif.bioinfo.pl/embedding.htm). The SVM (Yu-Dong ...
... the LFM. The negative instances were chosen randomly from those that do not include experimentally verified PTM of any type. These two datasets (positive and negative instances) were projected as sets of points in a multidimensional space (http://automotif.bioinfo.pl/embedding.htm). The SVM (Yu-Dong ...
1 - chem.msu.su
... The addition of a phosphate group to a Ser, Thr, or Tyr residue introduces a bulky, highly charged group into a region that was only moderately polar. When the modified side chain is located in a region of the protein critical to its three-dimensional structure, phosphorylation can be expected to ha ...
... The addition of a phosphate group to a Ser, Thr, or Tyr residue introduces a bulky, highly charged group into a region that was only moderately polar. When the modified side chain is located in a region of the protein critical to its three-dimensional structure, phosphorylation can be expected to ha ...
Differential Expression of Genes of the Calvin–Benson Cycle and its
... The mRNA levels of the Rubisco small subunit gene (RBCS) reach a maximum during leaf expansion but decrease rapidly with leaf age (Nikolau and Klessig 1987, Loza-Tavera et al. 1990, Glick et al. 1995, Miller et al. 2000, Suzuki et al. 2001, Suzuki et al. 2010). The genes of the A and B subunits of G ...
... The mRNA levels of the Rubisco small subunit gene (RBCS) reach a maximum during leaf expansion but decrease rapidly with leaf age (Nikolau and Klessig 1987, Loza-Tavera et al. 1990, Glick et al. 1995, Miller et al. 2000, Suzuki et al. 2001, Suzuki et al. 2010). The genes of the A and B subunits of G ...
Information. How to bring your samples
... concentration of nucleic acid. In traditional PCR, which is based on end-point detection, results are collected after the reaction is complete, making it impossible to determine the starting concentration of nucleic acid.Every real-time PCR contains a fluorescent reporter molecule—a TaqMan®probe or ...
... concentration of nucleic acid. In traditional PCR, which is based on end-point detection, results are collected after the reaction is complete, making it impossible to determine the starting concentration of nucleic acid.Every real-time PCR contains a fluorescent reporter molecule—a TaqMan®probe or ...
CHEMISTRY 112 - LECTURE NOTES
... amino acid sequence - DNA unwinds in nucleus; one DNA strand is preserved “as is” (coding strand) - the other DNA strand is used as pattern (template) to make mRNA strand which is complementary to template strand and identical to coding strand b) translation – mRNA code “rewritten” as amino acid seq ...
... amino acid sequence - DNA unwinds in nucleus; one DNA strand is preserved “as is” (coding strand) - the other DNA strand is used as pattern (template) to make mRNA strand which is complementary to template strand and identical to coding strand b) translation – mRNA code “rewritten” as amino acid seq ...
Biochemistry
... expression of genetic information must be cued to extrinsic signals and respond only when necessary. Mammalian cells possess about 1000 times more genetic information than does the bacterium Escherichia coli. Much of this additional genetic information is probably involved in regulation of gene expr ...
... expression of genetic information must be cued to extrinsic signals and respond only when necessary. Mammalian cells possess about 1000 times more genetic information than does the bacterium Escherichia coli. Much of this additional genetic information is probably involved in regulation of gene expr ...
A picorna-like virus from the red imported fire ant, Solenopsis invicta
... (h and U), and queens. The virus, tentatively named S. invicta virus (SINV-1), appears to belong to the picorna-like viruses. We did not observe any perceptible symptoms among infected nests in the field. However, in every case where an SINV-1-infected colony was excavated from the field with an ins ...
... (h and U), and queens. The virus, tentatively named S. invicta virus (SINV-1), appears to belong to the picorna-like viruses. We did not observe any perceptible symptoms among infected nests in the field. However, in every case where an SINV-1-infected colony was excavated from the field with an ins ...
Supplementary Material Legends
... Table S1. T-DNA insertion sites of target transgenes are defined by NCBI accession (acc.) numbers for left border or right border T-DNA - A. thaliana chromosomal DNA fusion sequences. Positions of nucleotides of A. thaliana genomic BAC sequences adjacent to left border and right border T-DNA sequenc ...
... Table S1. T-DNA insertion sites of target transgenes are defined by NCBI accession (acc.) numbers for left border or right border T-DNA - A. thaliana chromosomal DNA fusion sequences. Positions of nucleotides of A. thaliana genomic BAC sequences adjacent to left border and right border T-DNA sequenc ...
Selection at the Wobble Position of Codons Read by the Same tRNA
... carried out with the iterative use of the program FASTA (Pearson and Lipman 1988) set to standard parameters; a dedicated C language program was employed to conduct pairwise homology searches and to eliminate all the sequences having nucleotide sequence similarity scores with other yeast genes highe ...
... carried out with the iterative use of the program FASTA (Pearson and Lipman 1988) set to standard parameters; a dedicated C language program was employed to conduct pairwise homology searches and to eliminate all the sequences having nucleotide sequence similarity scores with other yeast genes highe ...
Appendices 1-5
... chains of cytochrome C oxidase, the terminal oxidase in mitochondrial electron transport. 8) Cytochrome C oxidase polypeptide VIII (Cox8b) is other of the nuclear-coded polypeptide chains of cytochrome C oxidase, the terminal oxidase in mitochondrial electron transport, and catalyzes the transfer of ...
... chains of cytochrome C oxidase, the terminal oxidase in mitochondrial electron transport. 8) Cytochrome C oxidase polypeptide VIII (Cox8b) is other of the nuclear-coded polypeptide chains of cytochrome C oxidase, the terminal oxidase in mitochondrial electron transport, and catalyzes the transfer of ...
The pk-1 gene of Autographa californica multinucleocapsid nuclear
... Lescott, 1984) and IE1 (J. Choi, personal communication; L. A. Guarino, unpublished results). Protein kinase activities are associated with baculovirus particles (Miller et al., 1983) and may even be essential for release of viral DNA from virions (Wilson & Consigli, 1985 a, b). In addition, the bac ...
... Lescott, 1984) and IE1 (J. Choi, personal communication; L. A. Guarino, unpublished results). Protein kinase activities are associated with baculovirus particles (Miller et al., 1983) and may even be essential for release of viral DNA from virions (Wilson & Consigli, 1985 a, b). In addition, the bac ...
Supplement Figures
... mainly based on optimizing the translation efficiency of highly express genes. Two recent papers [15, 16] used evolutionary selection for translation efficiency as a means to learn the efficiency and coefficients of codon-anticodon interactions. They found that some non-standard combinations seem to ...
... mainly based on optimizing the translation efficiency of highly express genes. Two recent papers [15, 16] used evolutionary selection for translation efficiency as a means to learn the efficiency and coefficients of codon-anticodon interactions. They found that some non-standard combinations seem to ...
Supplementary Methods - Word file (146 KB )
... A computer program was developed to select mRNA activation and deactivation tags, which were then realized using ssDNA molecules in most of our experiments. It accepts a set of mRNA sequences of the disease markers for a particular disease and provides the two most unique short subsequences for each ...
... A computer program was developed to select mRNA activation and deactivation tags, which were then realized using ssDNA molecules in most of our experiments. It accepts a set of mRNA sequences of the disease markers for a particular disease and provides the two most unique short subsequences for each ...
Figure S1
... Figure S4. Smyd2 regulates p53 responsive genes. a, Realtime PCR analysis of relative mRNA level of Smyd2 from BJ and BJ-DNp53 cells transfected with control (grey bar) or Smyd2 (striped bar) siRNA followed by adriamycin treatment for 8 hours. Results were the average from two independent experiment ...
... Figure S4. Smyd2 regulates p53 responsive genes. a, Realtime PCR analysis of relative mRNA level of Smyd2 from BJ and BJ-DNp53 cells transfected with control (grey bar) or Smyd2 (striped bar) siRNA followed by adriamycin treatment for 8 hours. Results were the average from two independent experiment ...
Replication-Coupled Packaging Mechanism in Positive
... FIG. 1. (A) Characteristic features of T-DNA constructs of FHV RNA. F1 and F2 constructs contain full-length cDNA copies of FHV genomic RNAs 1 (F1) and 2 (F2), respectively. Single lines and stippled boxes represent noncoding and coding regions, respectively. The position of the double 35S promoters ...
... FIG. 1. (A) Characteristic features of T-DNA constructs of FHV RNA. F1 and F2 constructs contain full-length cDNA copies of FHV genomic RNAs 1 (F1) and 2 (F2), respectively. Single lines and stippled boxes represent noncoding and coding regions, respectively. The position of the double 35S promoters ...
Re-identification of the N-terminal amino acid residue and its
... interpreted in terms of stability of proteins in a living cell. According to the N-end rule (Tobias et al. 1991; Varshavsky 1992), both Ala and Glu in bacteria are stabilizing residues that protect proteins against intracellular proteolytic degradation. The Ala residue does not prevent the removal o ...
... interpreted in terms of stability of proteins in a living cell. According to the N-end rule (Tobias et al. 1991; Varshavsky 1992), both Ala and Glu in bacteria are stabilizing residues that protect proteins against intracellular proteolytic degradation. The Ala residue does not prevent the removal o ...
Aphelenchoides besseyi
... sustain their life activities, plant parasitic nematodes have to obtain these metabolites from their hosts and the environment through the lipid binding proteins (LBPs) [4,5]. Nematodes have been found to produce a series of unusual proteins that exhibit high affinity binding to lipid, and these pro ...
... sustain their life activities, plant parasitic nematodes have to obtain these metabolites from their hosts and the environment through the lipid binding proteins (LBPs) [4,5]. Nematodes have been found to produce a series of unusual proteins that exhibit high affinity binding to lipid, and these pro ...
exam2review_s09.cwk (WP)
... Practice: The phosphoanhydride bonds of ATP are high in energy because a. of charge / charge repulsions between the negatively charged oxygen atoms of the phosphoanhydride groups. b. the products of hydrolysis are better solvated than ATP itself. c. the products of hydrolysis are more stable than AT ...
... Practice: The phosphoanhydride bonds of ATP are high in energy because a. of charge / charge repulsions between the negatively charged oxygen atoms of the phosphoanhydride groups. b. the products of hydrolysis are better solvated than ATP itself. c. the products of hydrolysis are more stable than AT ...
Minireview Shifty Ciliates: Frequent Programmed
... signal in euplotids. At first glance, this does not appear to be the case. Based on the analysis of 58 non-transposon, protein-coding genes, 87.9% of the Euplotes open reading frames (mainly predicted) have UAA as a terminator, and 37.9% end in UAA-A (Table 1). This raises the following conundrum: i ...
... signal in euplotids. At first glance, this does not appear to be the case. Based on the analysis of 58 non-transposon, protein-coding genes, 87.9% of the Euplotes open reading frames (mainly predicted) have UAA as a terminator, and 37.9% end in UAA-A (Table 1). This raises the following conundrum: i ...
Document
... Biochemical and Structural Characterization of RNA Modifying Enzymes RNA Uridine methyltransferase, RumA – Biochemical characterization of a RNA Uridine Methylatransfearse, RumA was done. This Methyltransferase contains a 4Fe-4S cluster and we are currently studying the role of this cluster. We have ...
... Biochemical and Structural Characterization of RNA Modifying Enzymes RNA Uridine methyltransferase, RumA – Biochemical characterization of a RNA Uridine Methylatransfearse, RumA was done. This Methyltransferase contains a 4Fe-4S cluster and we are currently studying the role of this cluster. We have ...
LIVER GENE EXPRESSION DURING THE TRANSITION DURING THE DRY PERIOD
... evidence implicates subclinical ketosis and fatty liver as predisposing factors for energyassociated disease as well as other metabolic and infectious problems. Function of the adult liver as well as the performance of essential functions in the body is controlled through the coordinated expression ...
... evidence implicates subclinical ketosis and fatty liver as predisposing factors for energyassociated disease as well as other metabolic and infectious problems. Function of the adult liver as well as the performance of essential functions in the body is controlled through the coordinated expression ...