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Lecture 10: Nucleic acids (DNA & RNA)
Lecture 10: Nucleic acids (DNA & RNA)

... viruses). 2) Ribonucleic acid (RNA): in some viruses, RNA serves as the genetic material.  Nucleic acids store and transmit genetic information ‫المعلومات الوراثية‬  Organisms inherit ‫ ترث‬DNA from their parents.  Each DNA molecule is very long and usually consists of hundreds to thousands of ge ...
answers
answers

... __JAMES WATSON____ & _FRANCIS CRICK_____ used _Rosalind Franklin’s X-ray images to help them figure out the structure of DNA. SUBUNIT PROTEINS ...
PPT
PPT

...  Strand which is complementary to the template strand  Strand of which the sequence is the same as that of the RNA transcript ...


... Steps • DNA Unzips (Hydrogen bonds break) • Each side acts as a template • New DNA nucleotides are added according to base-pairing rules • Two new molecules of DNA result – each with one old and one new strand. Happens in INTERPHASE (before mitosis or meiosis) ...
You Asked for it….. - Mr. Smith’s Science Page
You Asked for it….. - Mr. Smith’s Science Page

... Steps • DNA Unzips (Hydrogen bonds break) • Each side acts as a template • New DNA nucleotides are added according to base-pairing rules • Two new molecules of DNA result – each with one old and one new strand. Happens in INTERPHASE (before mitosis or meiosis) ...
No Slide Title
No Slide Title

... data points in multidimensional space. SOMS impose structure on a data set, clustering like data in “nodes”. GENECLUSTER: program developed to produce SOMS from microarray data:and available from these authors ...
Identification of the factors that interact with NCBP, an 80 kDa
Identification of the factors that interact with NCBP, an 80 kDa

... EGY48 was used some shifted bands were observed due to yeast endogenous proteins, however none of them were strictly dependent on the cap structure of the probe (lanes 1-3). We could not detect any additional band with extracts from yeast cells expressing only NCBP or NIPI (lanes 7-12). It is worth ...
when glucose is scarce
when glucose is scarce

... 1. A repressible operon is one that is usually on; binding of a repressor to the operator shuts off transcription. 1. The trp operon is a repressible operon. trp operon ...
QPCR Helpful Hints
QPCR Helpful Hints

... Once a standard curve has been generated it should be tested prior to running any unknown samples. The standard curve (run in duplicate) should generate an R2 value close to 1.0 and efficiency close to 100% (+/- 10%). The Nelson Lab typically uses 50 nM of forward and reverse primer the first time a ...
New Computational Tools Help Solve Puzzle of RNA Structure
New Computational Tools Help Solve Puzzle of RNA Structure

Information- Part 1 Study Guide
Information- Part 1 Study Guide

... via the lytic cycle. (C) Virus replication allows for mutations to occur through usual host pathways. (D) RNA viruses lack replication error-checking mechanisms, and thus have higher rates of mutation. (E) Related viruses can combine/recombine information if they infect the same host cell. (F) Genet ...
A1983RE63700001
A1983RE63700001

... quality of protein synthesis, is controlled by various metabolites which act at special regulatory genetic sites. “So when I spent most of my 1961 sabbatical leave with Sydney Brenner at the Cambridge Medical Research Council (MRC) Unit for Molecular Biology, it seemed an obvious question to ask whe ...
ch 17 from gene to protein
ch 17 from gene to protein

... • Enzymes in the eukaryotic nucleus modify premRNA (RNA processing) before the genetic messages are dispatched to the cytoplasm • During RNA processing, both ends of the primary transcript are usually altered • Also, usually some interior parts of the molecule are cut out, and the other parts splice ...
Gene Section RASL11B (RAS-like, family 11, member B) in Oncology and Haematology
Gene Section RASL11B (RAS-like, family 11, member B) in Oncology and Haematology

... size. Sizes of introns are 618 bp (intron 1), 1153 bp (intron 2), and 780 bp (exon 3). All splice sites have canonical boundaries, starting the intron with 'gt' and ending with 'ag'. A polyadenylation signal in the untranslated region of exon 4 is located at nucleotide position 1947. ...
ANSWERS - midterm study guide
ANSWERS - midterm study guide

... 12. What is a carrier? ______________________________________ Give an example of the genotype of a carrier.___ 13. What disease does someone have if they have Trisomy 21. ______________________________________________ What causes it? (Think about meiosis.) ___________________________________________ ...
Questions - Vanier College
Questions - Vanier College

... A) starts when the pathway's substrate is present. B) stops when the pathway's product is present. C) occurs continuously in the cell. D) does not result in the production of enzymes. E) starts when the pathway's product is present. 4. Gene expression might be altered at the level of post-transcript ...
II. Amino acid SEQUENCE
II. Amino acid SEQUENCE

... A. Amino acids do not have direct affinity for nucleic acids 1. Therefore, mRNA can not directly serve as a template for protein synthesis 2. There must exist “adapter molecules” which can read the RNA sequence (codons) and bring with it the correct amino acids a) This is the function of tRNA molecu ...
Ch27 PowerPoint LN
Ch27 PowerPoint LN

... salt concentration and DNA binding proteins ...
Pre-mRNA splicing: life at the centre of the central dogma
Pre-mRNA splicing: life at the centre of the central dogma

... apparent direct connection to splicing, still await functional characterization. Some of the additional proteins identified in these complexes have known associations with other aspects of RNA processing, including transcription and mRNA export. This is consistent with the intimate coupling of diffe ...
Part 1
Part 1

... sugar-phosphate backbones oriented in antiparallel fashion, result in DNA having the unique structural conformation often referred to as a “twisted ladder” (see Figure 2-2, B). Additionally, the dedicated base pairs provide the format essential for consistent replication and expression of the geneti ...
Chapter 17
Chapter 17

... • Transcription factors mediate the binding of RNA polymerase and the initiation of transcription • The completed assembly of transcription factors and RNA polymerase II bound to a promoter is called a transcription initiation complex • A promoter called a TATA box is crucial in forming the initiati ...
biochemistry-micromolecules
biochemistry-micromolecules

... • In DNA its DEOXYribose sugar • In RNA it’s Ribose sugar ...
problem set
problem set

... expression of the gene (Fig. 5.31). One common method by which expressed proteins are purified is via the attachment of an amino acid sequence such as a polyhistidine sequence (Histag) that serves as a tag for affinity purification. Mammalian cell expression systems offer the advantage that posttran ...
PowerPoint 演示文稿
PowerPoint 演示文稿

... Examples of gene action at the molecular levels ...
Presentation
Presentation

... RNA is the other type of nucleic acid. RNA stands for ribonucleic acid Its structure is a single strand of nucleotides RNA’s function is to decode genes within the DNA to make proteins 5. Like DNA, it has 4 nitrogenous bases – guanine and cytosine adenine and uracil ...
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