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CHIP-seq and RNA-seq
CHIP-seq and RNA-seq

... • Goal: to measure RNA levels of all genes in a genome under various experimental conditions • RNA levels vary with: ...
DNA and the Genome
DNA and the Genome

... to form a continuous sequence. This is called the mature transcript. The mature transcript then leaves the nucleus to travel to the cytoplasm. CFE Higher Biology ...
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... Viruses are not living and therefore viral diseases cannot be treated with antibiotics. Antiviral drugs such as AZT function by interfering with DNA replication. Viral genes (oncogenes) can cause some kinds of cancer. ...
AS 90729 version 2 Describe genetic processes Level 3 Credits 4
AS 90729 version 2 Describe genetic processes Level 3 Credits 4

... mechanisms for ensuring DNA stability o the effect of point mutations on gene expression DNA needs to be accurately replicated, as it codes for all the polypeptides a cell needs to function. It contains genes, which result in a sequence of amino acids and therefore gives the polypeptides their uniqu ...
PRE-AP Stage 3 – Learning Plan
PRE-AP Stage 3 – Learning Plan

... SCAFFOLD: Students will identify the components of DNA and describe how genetic information is carried in DNA. After identifying the components of the structure of DNA, students will explain how DNA is transcribed and translated into amino acids to make proteins. ACCELERATE: PREAP – purines, pyrimid ...
Learning Objectives
Learning Objectives

... 15. Explain the general process of transcription, including the three major steps of initiation, elongation, and termination. 16. Explain how RNA is modified after transcription in eukaryotic cells. 17. Define and explain the role of ribozymes. What three properties allow some RNA molecules to funct ...
Nucleic Acids and Protein Synthesis
Nucleic Acids and Protein Synthesis

... DNA using the complementary bases found in the nucleus of the cell –The fact that A only bonds with T and G only bonds with C means the new strand will be identical to the old separated strand • Each new DNA molecule consists of one old strand and one new strand ...
Learning Objectives
Learning Objectives

... 15. Explain the general process of transcription, including the three major steps of initiation, elongation, and termination. 16. Explain how RNA is modified after transcription in eukaryotic cells. 17. Define and explain the role of ribozymes. What three properties allow some RNA molecules to funct ...
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... with tRNA and radiolabeled RNA, then incubated with nuclear extract or recombinant proteins. For the Ran competition assay, 170 ng/l BSA or GST-Ran was pre-incubated with 50 ng/l RanBP2 ZFD, then incubated with labeled RNA and tRNA as described previously. EMSA was performed by native polyacrylami ...
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...  This allows the correct amino acid to be attached to the 3’ terminal by an enzyme called the tRNA activating enzyme.  There are 20 different tRNA activating enzymes (one for each of the 20 amino acids).  Each enzyme attaches one particular amino acids to all of the tRNA molecules that have an an ...
Biology Chapters 8 and 9 Test Review
Biology Chapters 8 and 9 Test Review

... o Once you find out what a human body’s genetic code sequence is, you can get cells to make things for you that can cure diseases. o Stem Cells are used for research and have no specialized function at the time. o AUG is a ‘start code’ that also stands for methionine. o UUU—phenylalanine. o UAA, UAG ...
Transcription and Translation
Transcription and Translation

... don’t appear in the final mRNA molecule. Protein-coding sections of a gene (called exons) are interrupted by introns. • The function of introns remains unclear. They may help is RNA transport or in control of gene expression in some cases, and they may make it easier for sections of genes to be shuf ...
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... BICH/GENE 431 KNOWLEDGE OBJECTIVES Chapter 13 – RNA Splicing Basic terminology: exon, intron, 5’ splice site, 3’ splice site Ave # of introns per gene increases from single-cell eukaryotes up through mammals In humans, ave. size of exon is 150 nt and ave. size of intron is 3000 nt Discovery of intro ...
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... Prions are the agents that cause mad cow disease (bovine spongiform encephalopathy), chronic wasting disease in deer and elk, scrapie in sheep, and Creutzfeld-Jakob syndrome in humans. These diseases cause neural degeneration. In humans, the symptoms are approximately those of Alzheimer’s syndrome a ...
Lecture genes to proteins translation - IIT
Lecture genes to proteins translation - IIT

... 1 When a ribosome reaches a stop 2 The release factor hydrolyzes 3 The two ribosomal subunits codon on mRNA, the A site of the the bond between the tRNA in and the other components of ribosome accepts a protein called the P site and the last amino the assembly dissociate. a release factor instead of ...
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... Chapt 8 Student learning outcomes Because proteins are the active players in most cell processes ...
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... It was observed that antisense or sense RNA could equally effect silencing (knock-down) of target gene expression ...
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Master Entrance Exam

... (B) In the presence of malonate, one would expect succinate to accumulate. (C) Oxaloacetate is used as a substrate but is not consumed in the cycle. (D) Succinate dehydrogenase channels electrons directly into the electron transfer chain. (E) The condensing enzyme is subject to allosteric regulation ...
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...  Genes: DNA segments that carry this information  Intron: part of gene not translated into protein, spliced out of mRNA (messenger RNA – conveys genetic info from DNA to ribosome where proteins are made)  Exon: mRNA translated into protein; protein consists only of exonderived sequences ...
THE DISCOVERY OF REVERSE TRANSCRIPTASE
THE DISCOVERY OF REVERSE TRANSCRIPTASE

... Temin firmly believed the activity existed. For him, it was the process of doing biochemical experiments on purified virions, rather than on infected cells, that allowed him to prove to the world what he knew. Baltimore, on the other hand, believed that viruses carried their polymerase activities wi ...
Genetics Module B, Anchor 2 Basic Mendelian Genetics: 1. Different
Genetics Module B, Anchor 2 Basic Mendelian Genetics: 1. Different

... Multple codons code for the same amino acid. Therefore, a mutation may change a base without changing the amino acid for which that codon codes. This would result in no change in the protein function. 7. One difference between a gene mutation and a chromosomal mutation is C. A chromosomal mutation ...
CHAPTER 15
CHAPTER 15

... C11. Answer: An anticodon that was 3–UUG–5 would recognize the two codons. To recognize 5–AAA–3, it would have to be modified to 3–UUI–5. C12. Answer: All tRNA molecules have some basic features in common. They all have a cloverleaf structure with three stem-loop structures. The second stem-lo ...
SUNY-ESF Web
SUNY-ESF Web

... 16S rRNA.. TATA Box– A conserved nucleotide sequence found in many eukaryotic promoters of structural genes found -35 of the initiation nucleotide. Unlike the Pribnow box, it is not necessary for RNA transcription but rather defines the initation codon for the mRNA.. Wobble Hypothesis (define it - ...
The Origin of Eukaryotic Cells
The Origin of Eukaryotic Cells

... colleages at the University of Illinois began a series of studies on different organisms, comparing the nucleotide sequence of the RNA molecule that resides in the small subunit of the ribosome. This RNA—which is called the 16S rRNA in prokaryotes or the 18S rRNA in eukaryotes—was chosen because it ...
practice exam 3_answer key
practice exam 3_answer key

... a. The 3 prime carbon is attached to a hydroxyl group b. The 3 prime carbon is attached to a phosphate group c. During DNA replication, neither strand grows from the 3 prime end d. The 3 prime end of one strand is next to (across from) the 3 prime end of the other strand e. None of the above are tru ...
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