Exam 2a - web.biosci.utexas.edu
... 1. (4 points) Bacterial cells can synthesize ATP by 3 different mechanisms. Some of these mechanisms depend on the regeneration of NAD + (oxidized form of NADH). Mention 1 mechanism by which a cell can regenerate NAD + in the absence of oxygen. For this mechanism, is the final electron acceptor an i ...
... 1. (4 points) Bacterial cells can synthesize ATP by 3 different mechanisms. Some of these mechanisms depend on the regeneration of NAD + (oxidized form of NADH). Mention 1 mechanism by which a cell can regenerate NAD + in the absence of oxygen. For this mechanism, is the final electron acceptor an i ...
The Basics: In Vitro Translation
... transcripts. Typically, eukaryotic mRNAs are characterized by two post-transcriptional modifications: a 5'-7 methyl-GTP cap and a 3' poly(A) tail. Both modifications contribute to the stability of the mRNA by preventing degradation. Additionally, the 5' cap structure enhances the translation of mRNA ...
... transcripts. Typically, eukaryotic mRNAs are characterized by two post-transcriptional modifications: a 5'-7 methyl-GTP cap and a 3' poly(A) tail. Both modifications contribute to the stability of the mRNA by preventing degradation. Additionally, the 5' cap structure enhances the translation of mRNA ...
LOYOLA COLLEGE (AUTONOMOUS), CHENNAI – 600 034
... 12. Describe any two inborn errors of carbohydrate metabolism. 13. Explain the role of different types of complexes involved in electron transport chain. 14. Explain the biosynthesis of saturated fatty acids. 15. Describe the Watson and Crick model of DNA. 16. Explain diagrammatically how the replic ...
... 12. Describe any two inborn errors of carbohydrate metabolism. 13. Explain the role of different types of complexes involved in electron transport chain. 14. Explain the biosynthesis of saturated fatty acids. 15. Describe the Watson and Crick model of DNA. 16. Explain diagrammatically how the replic ...
Final Review Answer Key - Mercer Island School District
... first step occurs in the nucleus . The second step is translation where the protein is actually produced. The process occurs in the ribosome. In this process each 3 nucleotide sequence in the mRNA, called a codon is paired with another type of RNA called tRNA which has a complementary anticodon . Ea ...
... first step occurs in the nucleus . The second step is translation where the protein is actually produced. The process occurs in the ribosome. In this process each 3 nucleotide sequence in the mRNA, called a codon is paired with another type of RNA called tRNA which has a complementary anticodon . Ea ...
AP Exam 5 Study Guide
... Frameshift Mutation- Occurs when there is an addition or deletion. Remember the frame is read in three letter chunks called codons. One additional, or one less throws the frame off and leads to the complete protein not being made. Point Mutation- Happens at a point in the DNA strand where one or a f ...
... Frameshift Mutation- Occurs when there is an addition or deletion. Remember the frame is read in three letter chunks called codons. One additional, or one less throws the frame off and leads to the complete protein not being made. Point Mutation- Happens at a point in the DNA strand where one or a f ...
Bchm 2000 Problem Set 3 Spring 2008 1. You
... the Michaelis-Menten equation: v0 = kcat [Etotal] [S] / (KM + [S]). Here, kcat = 10 s-1, [Etotal] = 1 µM and KM = 50 µM. Inserting the different values for [S] yields the following values for v0: for [S] = 25 µM (i.e. KM/2), v0 = 3.33 µM s-1; for [S] = 50 µM (i.e. KM), v0 = 5 µM s-1 = vmax/2 = kcat ...
... the Michaelis-Menten equation: v0 = kcat [Etotal] [S] / (KM + [S]). Here, kcat = 10 s-1, [Etotal] = 1 µM and KM = 50 µM. Inserting the different values for [S] yields the following values for v0: for [S] = 25 µM (i.e. KM/2), v0 = 3.33 µM s-1; for [S] = 50 µM (i.e. KM), v0 = 5 µM s-1 = vmax/2 = kcat ...
Chapter 5: Biological Molecules Molecules of Life • All life made up
... o Most go thru several states on way to a stable structure o Chaperonin – protein that assists in proper folding of other proteins Separate proteins from ‘bad’ influences in cytoplasm during folding Nucleic Acids DNA o Deoxyribonucleic acid o Made of genes, which form chromosomes o Direct amino ...
... o Most go thru several states on way to a stable structure o Chaperonin – protein that assists in proper folding of other proteins Separate proteins from ‘bad’ influences in cytoplasm during folding Nucleic Acids DNA o Deoxyribonucleic acid o Made of genes, which form chromosomes o Direct amino ...
Genetics - Purdue Physics
... DNA Stores information, and is replicated RNA contains information in DNA RNA is used to direct synthesis of ...
... DNA Stores information, and is replicated RNA contains information in DNA RNA is used to direct synthesis of ...
Lecture 2 - Cell assembly
... • Cytoplasm – everything inside the membrane • Nucleoid/Chromosome – DNA of the organism – it is not contained by a nuclear membrane (as eukaryote cell) • Ribosomes – made of ribosomal RNA and protein these are responsible for making proteins • Vacuoles or vesicles – spaces in the cytoplasm that c ...
... • Cytoplasm – everything inside the membrane • Nucleoid/Chromosome – DNA of the organism – it is not contained by a nuclear membrane (as eukaryote cell) • Ribosomes – made of ribosomal RNA and protein these are responsible for making proteins • Vacuoles or vesicles – spaces in the cytoplasm that c ...
Science - IIS E. Fermi
... The process by which DNA achieves its control of cell life and function through protein synthesis is called gene expression. A gene is a DNA sequence that contains genetic information for one functional protein. Proteins are essential for the modulation and maintenance of cellular activities. The am ...
... The process by which DNA achieves its control of cell life and function through protein synthesis is called gene expression. A gene is a DNA sequence that contains genetic information for one functional protein. Proteins are essential for the modulation and maintenance of cellular activities. The am ...
The Nucleolus
... The nucleolus is a bundle of chromatin, RNA, and proteins It has two distinct parts: Fibrillar and Granular Nucleoli form at Nucleolus Organizer Regions (NORs), ...
... The nucleolus is a bundle of chromatin, RNA, and proteins It has two distinct parts: Fibrillar and Granular Nucleoli form at Nucleolus Organizer Regions (NORs), ...
Ribosome Profiling Enables Comprehensive Translation
... profiling by examining protein synthesis in Saccharomyces cerevisiae that were grown under normal conditions and under the stress of amino acid starvation. The authors observed extensive translational control influencing protein abundance, differences in ribosome density between early and late pepti ...
... profiling by examining protein synthesis in Saccharomyces cerevisiae that were grown under normal conditions and under the stress of amino acid starvation. The authors observed extensive translational control influencing protein abundance, differences in ribosome density between early and late pepti ...
Example: search for regulatory binding sites
... the so-called promoter region (TATA-box; or -10, -35) – Regulated by some (regulatory) proteins on DNA “near” the promoter region. – These binding sites on DNA are often “similar” in composition. Enhancers and repressors ...
... the so-called promoter region (TATA-box; or -10, -35) – Regulated by some (regulatory) proteins on DNA “near” the promoter region. – These binding sites on DNA are often “similar” in composition. Enhancers and repressors ...
Ribosome structural studies
... highly energetic electrons is directed at the sample to provide up to 500,000X magnification. Invaluable topological, morphological, compositional, and crystallographic insight can be obtained. ...
... highly energetic electrons is directed at the sample to provide up to 500,000X magnification. Invaluable topological, morphological, compositional, and crystallographic insight can be obtained. ...
17_Learning_Objectives
... 6. Distinguish between transcription and translation. 7. Compare where transcription and translation occur in bacteria and in eukaryotes. 8. Define “codon” and explain the relationship between the linear sequence of codons on mRNA and the linear sequence of amino acids in a polypeptide. 9. Explain t ...
... 6. Distinguish between transcription and translation. 7. Compare where transcription and translation occur in bacteria and in eukaryotes. 8. Define “codon” and explain the relationship between the linear sequence of codons on mRNA and the linear sequence of amino acids in a polypeptide. 9. Explain t ...
DNA (Deoxyribonucleic Acid)
... This process is called Transcription. 2. On the factory floor, mRNA moves to the assembly line, a ribosome. ...
... This process is called Transcription. 2. On the factory floor, mRNA moves to the assembly line, a ribosome. ...
Identification of Target Messenger RNA Substrates for the Murine
... technique recently developed in our lab, we identified mRNAs from testis that were specifically bound by mDAZL. One mRNA encoded the Tpx-1 protein, a testicular cell adhesion protein essential for the progression of spermatogenesis. A 26-nucleotide region necessary and sufficient to bind mDAZL was f ...
... technique recently developed in our lab, we identified mRNAs from testis that were specifically bound by mDAZL. One mRNA encoded the Tpx-1 protein, a testicular cell adhesion protein essential for the progression of spermatogenesis. A 26-nucleotide region necessary and sufficient to bind mDAZL was f ...
Mutations Activity
... Introduction: DNA is genetic material made of nucleotides. Last unit we saw how proteins were created through transcription (DNAmRNA) and translation (mRNAlinked amino acids). However, in this unit we want to see how those processes can “go wrong” and create mutations. In this activity you will in ...
... Introduction: DNA is genetic material made of nucleotides. Last unit we saw how proteins were created through transcription (DNAmRNA) and translation (mRNAlinked amino acids). However, in this unit we want to see how those processes can “go wrong” and create mutations. In this activity you will in ...
Protein Structure - FAU College of Engineering
... Intron – DNA transcribed into mRNA but later removed Untranslated region (UTR) – DNA not expressed ...
... Intron – DNA transcribed into mRNA but later removed Untranslated region (UTR) – DNA not expressed ...
Introduction to Next Generation Sequencing
... • Determine the target genome sequence (i.e., repeat classes) • Mapping options • Number of allowed mis-matches (as function of position) • Number of mapped loci (e.g., 1 = unique read sequence) • Generate Consensus Sequence and identify SNPs • Generate Read Enrichment Profile (e.g., Wald Lab tool) ...
... • Determine the target genome sequence (i.e., repeat classes) • Mapping options • Number of allowed mis-matches (as function of position) • Number of mapped loci (e.g., 1 = unique read sequence) • Generate Consensus Sequence and identify SNPs • Generate Read Enrichment Profile (e.g., Wald Lab tool) ...
