DNA Microarray - School of Biotechnology
... • Linear amplification methods can be used to increase the amount of RNA so that microarray experiments can be performed using very small numbers of cells. It’s not clear to what degree this affects results, especially with respect to rare transcripts, but seems to be generally OK if done correctly ...
... • Linear amplification methods can be used to increase the amount of RNA so that microarray experiments can be performed using very small numbers of cells. It’s not clear to what degree this affects results, especially with respect to rare transcripts, but seems to be generally OK if done correctly ...
Transcription Coactivator Family Proteins
... [0003] Activation of transcription in eukaryotes depends upon the interplay between sequence specific transcriptional activators and general transcription factors. While direct contacts between activators and general factors have been demonstrated in vitro, an additional class of proteins, termed co ...
... [0003] Activation of transcription in eukaryotes depends upon the interplay between sequence specific transcriptional activators and general transcription factors. While direct contacts between activators and general factors have been demonstrated in vitro, an additional class of proteins, termed co ...
Lecture 17 Expanded Genetic Code
... But there is a problem: When you change the anticodon to CUA, the identity uniqueness is lost, and in addition to the Tyr . aaRS (archae) recognizing it, some E. coli aaRS also recognize and load the tRNA. The solution is to create a large library of tRNAs and use an in vitro selection scheme to id ...
... But there is a problem: When you change the anticodon to CUA, the identity uniqueness is lost, and in addition to the Tyr . aaRS (archae) recognizing it, some E. coli aaRS also recognize and load the tRNA. The solution is to create a large library of tRNAs and use an in vitro selection scheme to id ...
Topic 3 – The Chemistry of Life
... lower activity above and below optimum pH / graph showing this too acidic / base pH can determine enzyme change shape of active site / tertiary structure altered substrate cannot bind to active site / enzyme-substrate complex cannot hydrogen / ionic bonds in the enzyme / active site are br ...
... lower activity above and below optimum pH / graph showing this too acidic / base pH can determine enzyme change shape of active site / tertiary structure altered substrate cannot bind to active site / enzyme-substrate complex cannot hydrogen / ionic bonds in the enzyme / active site are br ...
Number 49, 2002 13 Balázs Csóka , Tamás Zeke
... BimG11 was described as a temperature-sensitive recessive mutation that causes the block of m itosis accompanied with the overphosphorylation of nuclear proteins and distinct morphological changes in Aspergillus nidulans (Doonan and Morris, 1989 Cell 57: 987996). In addition the mutant was defective ...
... BimG11 was described as a temperature-sensitive recessive mutation that causes the block of m itosis accompanied with the overphosphorylation of nuclear proteins and distinct morphological changes in Aspergillus nidulans (Doonan and Morris, 1989 Cell 57: 987996). In addition the mutant was defective ...
S4 Text
... Concentration of aa i (not incorporated in protein) Concentration of tRNA charged with aa i Concentration of free tRNA conjugate to aa i Total concentration of tRNA conjugate to aa i Total concentration of ribosome with an A-site for aa i Ribosomes with uncharged tRNA in an A-site for aa i Concentra ...
... Concentration of aa i (not incorporated in protein) Concentration of tRNA charged with aa i Concentration of free tRNA conjugate to aa i Total concentration of tRNA conjugate to aa i Total concentration of ribosome with an A-site for aa i Ribosomes with uncharged tRNA in an A-site for aa i Concentra ...
Help Wanted
... maintain genetic continuity through replication and control cellular activity by regulation of enzyme production. Limited number of openings. All benefits. Supervisor of production of proteins—all shifts. Must be able to follow exact directions from double-stranded template. Travel from nucleus to t ...
... maintain genetic continuity through replication and control cellular activity by regulation of enzyme production. Limited number of openings. All benefits. Supervisor of production of proteins—all shifts. Must be able to follow exact directions from double-stranded template. Travel from nucleus to t ...
RNA polymerase I
... • 8 - 14 distinct subunits; visible in EM • differ in sensitivities to a-amanitin, ...
... • 8 - 14 distinct subunits; visible in EM • differ in sensitivities to a-amanitin, ...
Gene Section RNF11 (ring finger protein 11) Atlas of Genetics and Cytogenetics
... anchoring via acylation is necessary in order for RNF11 to be post-translationally modified by the addition of several ubiquitin moieties (Santonico et al., 2010). Four serine (Ser) residues have been identified as murine RNF11 phosphorylation sites (Ser7, Ser14, Ser25, Ser54) with differences betwe ...
... anchoring via acylation is necessary in order for RNF11 to be post-translationally modified by the addition of several ubiquitin moieties (Santonico et al., 2010). Four serine (Ser) residues have been identified as murine RNF11 phosphorylation sites (Ser7, Ser14, Ser25, Ser54) with differences betwe ...
14 - Lab Times
... The Primordial Soup So, what kind of chemistry could have produced life? In 1924, Aleksandr Oparin, a young Soviet biochemist, argued that a primeval soup of organic molecules could have been created under the oxygen-less atmosphere of the early earth through the action of sunlight. The important po ...
... The Primordial Soup So, what kind of chemistry could have produced life? In 1924, Aleksandr Oparin, a young Soviet biochemist, argued that a primeval soup of organic molecules could have been created under the oxygen-less atmosphere of the early earth through the action of sunlight. The important po ...
Amiito acid sequence of the testosterone
... submaxillary gland are 1350, 1450, and 1950 nt In length (2,9). These same three mRNAs are expressed at low levels in brain and muscle, but the more abundant RP2 mRNAs in these tissues are 1050, 1150, and 1650 nt long (2, D.K., unpublished observations). All of these different mRNAs are produced by ...
... submaxillary gland are 1350, 1450, and 1950 nt In length (2,9). These same three mRNAs are expressed at low levels in brain and muscle, but the more abundant RP2 mRNAs in these tissues are 1050, 1150, and 1650 nt long (2, D.K., unpublished observations). All of these different mRNAs are produced by ...
How do viruses differ?
... a. averages 20 - 40 minutes b. the number of phage particles released from a single cell is referred to burst size Ranging from 50 to 200 particles D. Life cycle of a lysogenic phage Lysogeny is a state of cell chromosome where a bacteriophage genome has been inserted into the bacterial chromosome b ...
... a. averages 20 - 40 minutes b. the number of phage particles released from a single cell is referred to burst size Ranging from 50 to 200 particles D. Life cycle of a lysogenic phage Lysogeny is a state of cell chromosome where a bacteriophage genome has been inserted into the bacterial chromosome b ...
insight review articles
... with a frame shift of ~10 nucleotides. The second step of the degradation probably takes place exclusively in the cytoplasm, as silencing does not reduce the full-length transcript levels in the nucleus27. However, the first step could occur in both the cytoplasm and the nucleus. Many mRNA degradati ...
... with a frame shift of ~10 nucleotides. The second step of the degradation probably takes place exclusively in the cytoplasm, as silencing does not reduce the full-length transcript levels in the nucleus27. However, the first step could occur in both the cytoplasm and the nucleus. Many mRNA degradati ...
Measuring the Rates of Transcriptional Elongation in the Female
... the nuclear run-on assay readily detected an approximately 1.5-fold change in the transcriptional rate of a housekeeping gene in D. melanogaster ovaries. A central assumption in this assay is that only transcripts initiated by RNA pol II prior to cell lysis are extended during the radiolabeling reac ...
... the nuclear run-on assay readily detected an approximately 1.5-fold change in the transcriptional rate of a housekeeping gene in D. melanogaster ovaries. A central assumption in this assay is that only transcripts initiated by RNA pol II prior to cell lysis are extended during the radiolabeling reac ...
MagNA Lyser Instrument
... Rarely expressed targets in small numbers of target cells, as seen in experiments about minimal residual diseases, are difficult to detect. Increasing the cell number can improve sensitivity and lead to accurate results. Without the MagNA Lyser preprocessing, the MagNA Pure mRNA HS Kit can efficient ...
... Rarely expressed targets in small numbers of target cells, as seen in experiments about minimal residual diseases, are difficult to detect. Increasing the cell number can improve sensitivity and lead to accurate results. Without the MagNA Lyser preprocessing, the MagNA Pure mRNA HS Kit can efficient ...
blank worksheet
... Specialist software: Which (if any) additional software was used? Self-developed algorithms, or other (please specify) ...
... Specialist software: Which (if any) additional software was used? Self-developed algorithms, or other (please specify) ...