COMPUTATIONAL MODELING OF CHARGE TRANSFER IN NUCLEOBASE-AROMATIC AMINO ACID COMPLEXES Cristina BUTCHOSA ROBLES
... states are also called electron ”holes”. Electron holes can migrate long distances through the nucleobases stack, due to conductivity properties of DNA. Finally, the cationic charge could be trapped and most probably a mutagenic lesion will be initiated. However, if DNA interacts with a protein or p ...
... states are also called electron ”holes”. Electron holes can migrate long distances through the nucleobases stack, due to conductivity properties of DNA. Finally, the cationic charge could be trapped and most probably a mutagenic lesion will be initiated. However, if DNA interacts with a protein or p ...
ARTICLES - Weizmann Institute of Science
... expressed genes. Consistent with this expectation, the highly expressed ribosomal RNA and transfer RNA genes stood out as having markedly low predicted nucleosome occupancy. In contrast to the ubiquitously expressed tRNAs, many other genes vary their expression between high and low levels in differe ...
... expressed genes. Consistent with this expectation, the highly expressed ribosomal RNA and transfer RNA genes stood out as having markedly low predicted nucleosome occupancy. In contrast to the ubiquitously expressed tRNAs, many other genes vary their expression between high and low levels in differe ...
The demonstration of nickel in the urease of Helicobacter pylori by
... urease is greater than 500 kDa, then the suggested structure is hexameric. This would indicate that the probable number of nickel atoms is six. These results support the model of six copies of each of the two polypeptides in the native enzyme suggested by Hu and Mobley [11]. The detection of nickel ...
... urease is greater than 500 kDa, then the suggested structure is hexameric. This would indicate that the probable number of nickel atoms is six. These results support the model of six copies of each of the two polypeptides in the native enzyme suggested by Hu and Mobley [11]. The detection of nickel ...
Rabbit Reticulocyte Lysate Technical Manual
... poorly. Usually such preparations yield no better than 20–30% of the maximum incorporation attainable, and high final concentrations of 100–200µg/ml of RNA are needed to stimulate translation. In contrast, viral RNAs and poly(A)+ mRNAs (including mRNA transcribed in vitro) can be used at much lower ...
... poorly. Usually such preparations yield no better than 20–30% of the maximum incorporation attainable, and high final concentrations of 100–200µg/ml of RNA are needed to stimulate translation. In contrast, viral RNAs and poly(A)+ mRNAs (including mRNA transcribed in vitro) can be used at much lower ...
Prediction and investigation of novel proteins in DNA double
... Ottawa-Carleton Institute of Biology Carleton University Ottawa, Ontario ...
... Ottawa-Carleton Institute of Biology Carleton University Ottawa, Ontario ...
2004-011: Draft Annex to ISPM 27:2006 – Xanthomonas citri subsp
... Aliquots of 25 µl of each bacterial preparation or plant sample to be tested are pipetted onto a plastic-coated multi-window microscope slide, allowed to air dry and then gently heat-fixed over a flame. Separate slides are set up for each test bacterium, and also for positive and negative controls a ...
... Aliquots of 25 µl of each bacterial preparation or plant sample to be tested are pipetted onto a plastic-coated multi-window microscope slide, allowed to air dry and then gently heat-fixed over a flame. Separate slides are set up for each test bacterium, and also for positive and negative controls a ...
Polymerase Chain Reaction In Ophthalmology
... • PCR cannot detect the organism for which primers have not been provided. So a narrow and well defined differential diagnosis is required for PCR to be effectively useful. PCR and Koch's postulates 6, 7 Koch's postulates include isolation of suspected pathogen from all cases of a disease, successfu ...
... • PCR cannot detect the organism for which primers have not been provided. So a narrow and well defined differential diagnosis is required for PCR to be effectively useful. PCR and Koch's postulates 6, 7 Koch's postulates include isolation of suspected pathogen from all cases of a disease, successfu ...
Maintenance of genomic integrity by p53: complementary
... result, DNA damage will be repaired during a growth arrest, or the damaged cells will be eliminated, thereby preventing ®xation of DNA damage as mutations. This function of p53 led to the now famous coining of p53 as the `guardian of the genome' by Lane (1992). Although the main features of p53's ro ...
... result, DNA damage will be repaired during a growth arrest, or the damaged cells will be eliminated, thereby preventing ®xation of DNA damage as mutations. This function of p53 led to the now famous coining of p53 as the `guardian of the genome' by Lane (1992). Although the main features of p53's ro ...
File
... In 1952, Alfred D. Hershey and Martha Chase provided further evidence to support Avery et al.'s claims by demonstrating that DNA is the genetic material of a phage known as T2. T2 has a very simple structure, consisting of little more than DNA surrounded by a protein coat. T2 reproduces by inserting ...
... In 1952, Alfred D. Hershey and Martha Chase provided further evidence to support Avery et al.'s claims by demonstrating that DNA is the genetic material of a phage known as T2. T2 has a very simple structure, consisting of little more than DNA surrounded by a protein coat. T2 reproduces by inserting ...
Post-PCR sterilization: a method to control carryover contamination
... reagent should have additional properties to be used conveniently with the PCR process. First, the non-activated reagent must not interfere with primer annealing or Taq polymerase activity, and must be thermally stable to the temperatures encountered with the PCR technique. This permits the reagent ...
... reagent should have additional properties to be used conveniently with the PCR process. First, the non-activated reagent must not interfere with primer annealing or Taq polymerase activity, and must be thermally stable to the temperatures encountered with the PCR technique. This permits the reagent ...
Specialized Transduction by Bacteriophage P22 in Salmonella typhimurium: Genetic and Physical Structure of the Transducing Genomes and the Prophage Attachment Site.
... we found that DNA molecules from P22pro-I and P22pro-3 each contain a substitution which adds length to the composite genome making the intracellular replicated genome too long to fit into a single phage particle. In this respect, and in many of their biological properties, the proline-transducing p ...
... we found that DNA molecules from P22pro-I and P22pro-3 each contain a substitution which adds length to the composite genome making the intracellular replicated genome too long to fit into a single phage particle. In this respect, and in many of their biological properties, the proline-transducing p ...
DNA
... More Terminology • The genome is an organism’s complete set of DNA • a bacteria contains about 600,000 DNA base pairs • human and mouse genomes have some 3 billion ...
... More Terminology • The genome is an organism’s complete set of DNA • a bacteria contains about 600,000 DNA base pairs • human and mouse genomes have some 3 billion ...
"Electromagnetic Interactions Between Fluorescent Dipoles and Metal Nanoparticles"
... coefficients of the peak absorption at 520 and slightly red of the peak at 570nm .........................34 Figure 2.10 Reaction mechanism for citrate reduction and subsequent ligand exchange of AuNP.................................................................................................... ...
... coefficients of the peak absorption at 520 and slightly red of the peak at 570nm .........................34 Figure 2.10 Reaction mechanism for citrate reduction and subsequent ligand exchange of AuNP.................................................................................................... ...
Molecular mechanics of the interactions of spermine with DNA: DNA
... absolute values of energy for each sequence and each complex cannot be compared directly. For each case, both the DNA/spermine complex and the DNA from the complex after energy minimization were compared with DNA energy minimized in the absence of spermine; this allows both the stability of the comp ...
... absolute values of energy for each sequence and each complex cannot be compared directly. For each case, both the DNA/spermine complex and the DNA from the complex after energy minimization were compared with DNA energy minimized in the absence of spermine; this allows both the stability of the comp ...
New peptide and gene coding for same
... column. The poly (A) RNA were used to prepare a cDNA library according to the Okayama-Berg method (Mol. Cell. Biol. 2, 161-170, 1982). The library was screened with a mixture of probes consisting of synthesized 14 meroligonucleotides labeled with 32P coding for an amino acid sequence of from 12 to 1 ...
... column. The poly (A) RNA were used to prepare a cDNA library according to the Okayama-Berg method (Mol. Cell. Biol. 2, 161-170, 1982). The library was screened with a mixture of probes consisting of synthesized 14 meroligonucleotides labeled with 32P coding for an amino acid sequence of from 12 to 1 ...
Quantitative Analysis of the Kinetics of End
... structure. The ATP concentration is maintained at high levels so that each DNA-bound RecA monomer is functioning at its kcat. The kcat employed is that observed in the presence of longer random sequence ssDNA molecules. Under conditions used in this series of experiments, the kcat for ATP hydrolysis ...
... structure. The ATP concentration is maintained at high levels so that each DNA-bound RecA monomer is functioning at its kcat. The kcat employed is that observed in the presence of longer random sequence ssDNA molecules. Under conditions used in this series of experiments, the kcat for ATP hydrolysis ...
Analysis of Cross Sequence Similarities for Multiple - PolyU
... referencing to each other. We explore similarities between different chromosomes of the sequence ‘Saccharomyces cerevisiae’. These similarities are characterised by the existence of similar subsequences among different chromosomes. The longer the similar subsequences are, the higher the cross-simila ...
... referencing to each other. We explore similarities between different chromosomes of the sequence ‘Saccharomyces cerevisiae’. These similarities are characterised by the existence of similar subsequences among different chromosomes. The longer the similar subsequences are, the higher the cross-simila ...
Corning® Epoxide Coated Slides Instruction Manual
... be used in human diagnostics or for drug purposes, nor is it to be administered to humans in any way. This product contains chemicals that may be harmful if misused. Proper care should be exercised with this product to prevent human contact. Corning products are guaranteed to perform as described wh ...
... be used in human diagnostics or for drug purposes, nor is it to be administered to humans in any way. This product contains chemicals that may be harmful if misused. Proper care should be exercised with this product to prevent human contact. Corning products are guaranteed to perform as described wh ...
Gel electrophoresis of nucleic acids
Nucleic acid electrophoresis is an analytical technique used to separate DNA or RNA fragments by size and reactivity. Nucleic acid molecules which are to be analyzed are set upon a viscous medium, the gel, where an electric field induces the nucleic acids to migrate toward the anode, due to the net negative charge of the sugar-phosphate backbone of the nucleic acid chain. The separation of these fragments is accomplished by exploiting the mobilities with which different sized molecules are able to pass through the gel. Longer molecules migrate more slowly because they experience more resistance within the gel. Because the size of the molecule affects its mobility, smaller fragments end up nearer to the anode than longer ones in a given period. After some time, the voltage is removed and the fragmentation gradient is analyzed. For larger separations between similar sized fragments, either the voltage or run time can be increased. Extended runs across a low voltage gel yield the most accurate resolution. Voltage is, however, not the sole factor in determining electrophoresis of nucleic acids.The nucleic acid to be separated can be prepared in several ways before separation by electrophoresis. In the case of large DNA molecules, the DNA is frequently cut into smaller fragments using a DNA restriction endonuclease (or restriction enzyme). In other instances, such as PCR amplified samples, enzymes present in the sample that might affect the separation of the molecules are removed through various means before analysis. Once the nucleic acid is properly prepared, the samples of the nucleic acid solution are placed in the wells of the gel and a voltage is applied across the gel for a specified amount of time.The DNA fragments of different lengths are visualized using a fluorescent dye specific for DNA, such as ethidium bromide. The gel shows bands corresponding to different nucleic acid molecules populations with different molecular weight. Fragment size is usually reported in ""nucleotides"", ""base pairs"" or ""kb"" (for thousands of base pairs) depending upon whether single- or double-stranded nucleic acid has been separated. Fragment size determination is typically done by comparison to commercially available DNA markers containing linear DNA fragments of known length.The types of gel most commonly used for nucleic acid electrophoresis are agarose (for relatively long DNA molecules) and polyacrylamide (for high resolution of short DNA molecules, for example in DNA sequencing). Gels have conventionally been run in a ""slab"" format such as that shown in the figure, but capillary electrophoresis has become important for applications such as high-throughput DNA sequencing. Electrophoresis techniques used in the assessment of DNA damage include alkaline gel electrophoresis and pulsed field gel electrophoresis.For short DNA segments such as 20 to 60 bp double stranded DNA, running them in Polyacrylamide gel (PAGE) will give better resolution(native condition). Similarly, RNA and single stranded DNA can be run and visualised by PAGE gels containing denaturing agents such as Urea. PAGE gels are widely used in techniques such as DNA foot printing, EMSA and other DNA-protein interaction techniques.The measurement and analysis are mostly done with a specialized gel analysis software. Capillary electrophoresis results are typically displayed in a trace view called an electropherogram.