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Gene Section RAD52 (RAD52 homolog (S. cerevisiae)) Atlas of Genetics and Cytogenetics
Gene Section RAD52 (RAD52 homolog (S. cerevisiae)) Atlas of Genetics and Cytogenetics

... ability to stimulate homologous pairing by hRAD51 (Benson et al., 1998). The interaction of ScRAD52 and hRAD52 with replication protein A (RPA) is important for the binding with ssDNA by RAD52 (Hays et al., 1998; Shinohara et al., 1998; Jackson et al., 2002). hRAD52 binds directly to DSBs, protects ...
Chromosomal Genetics
Chromosomal Genetics

... If these two genes were on different chromosomes, the alleles from the F1 dihybrid would sort into gametes independently, and we would expect to see equal numbers of the four types of offspring. If these two genes were on the same chromosome, we would expect each allele combination, B+ vg+ and b vg, ...
Use of a single primer to fluorescently label selective amplified
Use of a single primer to fluorescently label selective amplified

... polymorphism (AFLP) markers are used for a variety of genetic applications including population genetic studies (1,2), mapping (3), and gene discovery (4). Genomic DNA or cDNA is digested with restriction enzymes in the presence of synthetic adaptors in a “restriction/ligation” reaction that produce ...
The cytogenetics of homologous chromosome pairing in meiosis in
The cytogenetics of homologous chromosome pairing in meiosis in

... meiotic prophase I and coincides with two other major meiotic processes: recombination and synapsis. Recombination starts by formation of double-strand breaks (DSBs) in chromosomal DNA, which are later repaired, leading to crossovers between a single sister chromatid of each homologous chromosome. S ...
A Genetic Linkage Map for the Zebrafish
A Genetic Linkage Map for the Zebrafish

The cytogenetics of homologous chromosome pairing in meiosis in plants Meiosis
The cytogenetics of homologous chromosome pairing in meiosis in plants Meiosis

... meiotic prophase I and coincides with two other major meiotic processes: recombination and synapsis. Recombination starts by formation of double-strand breaks (DSBs) in chromosomal DNA, which are later repaired, leading to crossovers between a single sister chromatid of each homologous chromosome. S ...
For example, Gall diseases on the roots of tobacco plants were first
For example, Gall diseases on the roots of tobacco plants were first

... same orientation. A number of housekeeping genes are located between the linear chromosome's rRNA operons, and one might expect frequent recombination resulting in lethal events. The linear chromosome is covalently closed linear molecule so the telomeres of the linear chromosome are covantly closed. ...
8-Cell and Molecular Biology (Transcription)
8-Cell and Molecular Biology (Transcription)

...  While proteins that have little to do with each other in the cells, their genes are adjacent  Therefore, in brief decoding genomes is not a simple matter  Even with the aid of powerful computers, it is still difficult for researchers • to locate definitively the beginning and end of genes in the ...
grade 12 life sciences learner notes
grade 12 life sciences learner notes

... GAUTENG DEPARTMENT OF EDUCATION SENIOR SECONDARY INTERVENTION PROGRAMME ...
Chapter 12
Chapter 12

... In the absence of ß-galactosides, the lac operon is expressed only at a very low (basal) level. Addition of specific ß-galactosides induces transcription of all three genes of the operon. The lac mRNA is extremely unstable; as a result, induction can be rapidly reversed. The same types of systems th ...
Cytogenetics to Cytogenomics: An Introduction to Genomic
Cytogenetics to Cytogenomics: An Introduction to Genomic

... monosomies, trisomies, chromosomal rearrangements, and large deletions or duplications. However, these methods are limited by low resolution or narrow target range. For example, karyotyping is capable of detecting only large chromosomal changes (typically > 5 Mb).2 It is a subjective technique, and ...
Biochemistry
Biochemistry

... Messenger RNA is synthesized in the cell nucleus by transcription of DNA, a process similar to DNA replication. As in replication, a small section of the DNA double helix unwinds, and the bases on the two strands are exposed. RNA nucleotides (ribonucleotides) line up in the proper order by hydrogen- ...
Identification and analysis of functional elements in 1% of the human
Identification and analysis of functional elements in 1% of the human

... • A group of genes encoding transcription factors that are critical in driving islet cell development in pancreas are newly discovered. • Sequence counts for this group are modest but comfortably above the threshold of 13. • The authors are able to provide strong arguments on the significance of thi ...
Solving Multiple Sequence Alignment Problems using Various E
Solving Multiple Sequence Alignment Problems using Various E

... one strand of a DNA double helix, it is a simple matter to figure out the sequence of bases on the other strand. DNA's unique structure enables the molecule to copy itself during cell division. When a cell prepares to divide, the DNA helix splits down the middle and becomes two single strands. These ...
Educator's Resource Guide 4226  Biology 1 s 4-5
Educator's Resource Guide 4226 Biology 1 s 4-5

... ▶ Fertilization is the process in which reproductive cells (egg from the female and sperm from the male) join to produce a new cell. ▶ A trait is a specific characteristic, such as (in peas) seed color or plant height. ▶ Mendel prevented self-pollination in the peas. He controlled fertilization so h ...
Gene expression: Transcription
Gene expression: Transcription

... One of their primary attributes is that they enable the differential splicing of different exons, result in different protein variants with different functional properties. ...
Chapter 1 Powerpoint SCC
Chapter 1 Powerpoint SCC

... Genomics: Large-Scale Analysis of DNA Sequences •  An organism’s genome is its entire set of genetic instructions •  The human genome and those of many other organisms have been sequenced using DNAsequencing machines •  Genomics is the study of sets of genes within and between species ...
08 May 21st Wang:07 Dummy - Plant Transformation Facility
08 May 21st Wang:07 Dummy - Plant Transformation Facility

... traits of interest in parent lines and incorporates them into a new variety through crosses (or hybridization). The resulting product can be further crossed to itself or backcrossed to another line of interest, thus further integrating the trait in the genetic background of choice. This approach, ho ...
Efficient TALEN-mediated gene targeting of chicken
Efficient TALEN-mediated gene targeting of chicken

... 2003)). Chicken vasa homologue (CVH) (DDX4) marks the chicken germ cell lineage at the earliest stages of embryonic development and therefore is hypothesised to be a maternal determinant for formation of the germ cell lineage (Tsunekawa et al., 2000). As a consequence, we would expect vasa to play ...
Additional file 4 - Springer Static Content Server
Additional file 4 - Springer Static Content Server

... Typically a tetraploid cell arises from a normal diploid 46,XX or 46,XY cell with AA, AB, or BB single nucleotide polymorphic sites. This diploid cell fails to divide resulting in a tetraploid cell with a 92,XXXX or 92,XXYY karyotype and AAAA, AABB, or BBBB single nucleotide polymorphic sites. All t ...
A-level Human Biology Question paper Unit 2 - Making Use of
A-level Human Biology Question paper Unit 2 - Making Use of

... (iii) Name the type of bond found at Y. ...
Exam 2 Review Guide November 8, 2014, 12:30 pm to 2:30 pm
Exam 2 Review Guide November 8, 2014, 12:30 pm to 2:30 pm

... Know what metabolism is Know the difference between catabolic and anabolic reactions Understand where in a biological molecule that energy is stored Know what ATP is including what it stands for, and where high level energy is kept in the molecules Understand the connection between ADP and ATP Know ...
Chapter 2
Chapter 2

... Chromosomal rearrangements can occur after chromosomes break. Which of the following statements are most accurate with respect to alterations in chromosome structure? a) Chromosomal rearrangements are more likely to occur in mammals than in other vertebrates. b) Translocations and inversions are no ...
Genetic Code and Transcription
Genetic Code and Transcription

... Met Phe His Asn Gly Lys Val (stop) ...
Regulatory sequences
Regulatory sequences

... /gene="text" /label=feature_label /map="text" /note="text" /number=unquoted /phenotype="text" /product="text" /pseudo /standard_name="text" /usedin=accnum:feature_label Comments this key should not be used when the need is merely to mark a region in order to comment on it or to use it in another fea ...
< 1 ... 91 92 93 94 95 96 97 98 99 ... 766 >

Cre-Lox recombination



In the field of genetics, Cre-Lox recombination is known as a site-specific recombinase technology, and is widely used to carry out deletions, insertions, translocations and inversions at specific sites in the DNA of cells. It allows the DNA modification to be targeted to a specific cell type or be triggered by a specific external stimulus. It is implemented both in eukaryotic and prokaryotic systems.The system consists of a single enzyme, Cre recombinase, that recombines a pair of short target sequences called the Lox sequences. This system can be implemented without inserting any extra supporting proteins or sequences. The Cre enzyme and the original Lox site called the LoxP sequence are derived from bacteriophage P1.Placing Lox sequences appropriately allows genes to be activated, repressed, or exchanged for other genes. At a DNA level many types of manipulations can be carried out. The activity of the Cre enzyme can be controlled so that it is expressed in a particular cell type or triggered by an external stimulus like a chemical signal or a heat shock. These targeted DNA changes are useful in cell lineage tracing and when mutants are lethal if expressed globally.The Cre-Lox system is very similar in action and in usage to the FLP-FRT recombination system.
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