From Gene To You
... DNA Level Genome is plastic (changeable) in ways that affect availability of specific genes for expression Some genes only work in certain cells, at certain time, in certain ...
... DNA Level Genome is plastic (changeable) in ways that affect availability of specific genes for expression Some genes only work in certain cells, at certain time, in certain ...
Lab 5 minipreps
... There are a number of techniques for isolating plasmid DNA. Most labs have adopted one of the spin column kits on the market. These are fast and reliable. For DNA purification, we will use anion-exchange resin/ spin column technique available through Qiagen (Santa Clarita, CA). It is based on the al ...
... There are a number of techniques for isolating plasmid DNA. Most labs have adopted one of the spin column kits on the market. These are fast and reliable. For DNA purification, we will use anion-exchange resin/ spin column technique available through Qiagen (Santa Clarita, CA). It is based on the al ...
DNA, RNA and Proteins
... Proteins called DNA polymerases catalyze the formation of the DNA molecule. The polymerases add nucleotides that pair with each base to form two new double helixes. DNA polymerases also have a “proofreading” function. During DNA replication, errors sometimes occur, and the wrong nucleotide is added ...
... Proteins called DNA polymerases catalyze the formation of the DNA molecule. The polymerases add nucleotides that pair with each base to form two new double helixes. DNA polymerases also have a “proofreading” function. During DNA replication, errors sometimes occur, and the wrong nucleotide is added ...
pdb-d.eng.uiowa.edu
... Other sequences around CAAT box probably influence what actually gets bound to the CAAT box A conserved DNA element does not necessarily imply that the same regulatory element is binding. ...
... Other sequences around CAAT box probably influence what actually gets bound to the CAAT box A conserved DNA element does not necessarily imply that the same regulatory element is binding. ...
Gene Cloning
... • Restriction enzymes cut covalent phosphodiester bonds of both strands, often in a staggered way creating single-stranded ends, sticky ends. – These extensions will form hydrogen-bonded base pairs with complementary single-stranded stretches on other DNA molecules cut with the same restriction enzy ...
... • Restriction enzymes cut covalent phosphodiester bonds of both strands, often in a staggered way creating single-stranded ends, sticky ends. – These extensions will form hydrogen-bonded base pairs with complementary single-stranded stretches on other DNA molecules cut with the same restriction enzy ...
DNA to RNA practice
... Using the base pairing rules, find the anticodons for the template strand. A T C G TA G C Practice: Use these top strands of DNA and convert them into two strands. ...
... Using the base pairing rules, find the anticodons for the template strand. A T C G TA G C Practice: Use these top strands of DNA and convert them into two strands. ...
BIO 132: Genes and People
... generated from those sequences (some will change the protein, others will not) Exam 3: Problems DNA sequences will be provided and students will have to illustrate the protein product from the sequence ...
... generated from those sequences (some will change the protein, others will not) Exam 3: Problems DNA sequences will be provided and students will have to illustrate the protein product from the sequence ...
Welcome to the Chapter 12 Test!
... • Discuss each answer before writing! • During the discussion, decide which concepts from the notes to include in your answer. • Remember to underline each concept. • If you can’t whisper, you will work alone. ...
... • Discuss each answer before writing! • During the discussion, decide which concepts from the notes to include in your answer. • Remember to underline each concept. • If you can’t whisper, you will work alone. ...
Spring Semester - Final Exam Review Guide (BIO I Version)
... 62. Which blood type is the universal receiver? Donor? 63. A man who is homozygous for blue eyes marries a women who is heterozygous for brown eyes. What are the phenotypic and genotypic ratios of their offspring? 64. Black fur is dominant to white fur in Cocker Spaniel dogs, however a heterozygous ...
... 62. Which blood type is the universal receiver? Donor? 63. A man who is homozygous for blue eyes marries a women who is heterozygous for brown eyes. What are the phenotypic and genotypic ratios of their offspring? 64. Black fur is dominant to white fur in Cocker Spaniel dogs, however a heterozygous ...
5-5-17-Cloning_Plasmids_with_Paper
... These are needed to transcribe the gene properly when it is read. In addition, the HindIII & EcoR1 restriction enzyme cutting sites (sequences of bases) are marked in bold on the Jellyfish Glo gene DNA. The two restriction enzymes and their respective restriction sites are listed below. These enzyme ...
... These are needed to transcribe the gene properly when it is read. In addition, the HindIII & EcoR1 restriction enzyme cutting sites (sequences of bases) are marked in bold on the Jellyfish Glo gene DNA. The two restriction enzymes and their respective restriction sites are listed below. These enzyme ...
10/02 Chromatin and Chromosome structure
... Concept Check 3 Neutralizing their positive charges would have which effect on the histone proteins? a. b. c. d. ...
... Concept Check 3 Neutralizing their positive charges would have which effect on the histone proteins? a. b. c. d. ...
level two biology: gene expression
... I can define DNA in terms of the information it encodes. I can define RNA by using the terms ‘single-stranded’, ‘copy’ and ‘gene’. I can explain why RNA is necessary for protein synthesis by comparing the size and importance of RNA and DNA. I can show that I understand the processes of transcription ...
... I can define DNA in terms of the information it encodes. I can define RNA by using the terms ‘single-stranded’, ‘copy’ and ‘gene’. I can explain why RNA is necessary for protein synthesis by comparing the size and importance of RNA and DNA. I can show that I understand the processes of transcription ...
Opportunities for Theory in Biological Physics. 1) Chromosome
... How is the “open” architecture of the nucleus maintained and controlled under the osmotic pressure of de-condensed, active DNA sections. Equation of State of DNA bundles is known. Dynamics: Chromosome dynamics driven by DNA condensation/de-condensation events triggered by local gene expression:”gene ...
... How is the “open” architecture of the nucleus maintained and controlled under the osmotic pressure of de-condensed, active DNA sections. Equation of State of DNA bundles is known. Dynamics: Chromosome dynamics driven by DNA condensation/de-condensation events triggered by local gene expression:”gene ...
make a mammal project
... Standards: Cell Biology: 1. Life depends on many chemical reactions that happen in specific parts of cells called Organelles. To understand this, you must know: CB1d: the main idea (Central Dogma) of molecular biology shows how information moves from DNA to RNA during Transcription, and Translates i ...
... Standards: Cell Biology: 1. Life depends on many chemical reactions that happen in specific parts of cells called Organelles. To understand this, you must know: CB1d: the main idea (Central Dogma) of molecular biology shows how information moves from DNA to RNA during Transcription, and Translates i ...
DNA, RNA and Protein
... This process produces two exact DNA molecules (Chromosomes) that are the same DNA never leaves the nucleus ...
... This process produces two exact DNA molecules (Chromosomes) that are the same DNA never leaves the nucleus ...
PLASMID ISOLATIONS (MINIPREPS)
... To obtain highly purified plasmid DNA, there are two basic methods to use. First is to use column chromatography, many commercial columns are available (HPLC can also be used). The second is to use ethidium bromide in cesium chloride gradients. Ethidium bromide can fit between the stacked bases in D ...
... To obtain highly purified plasmid DNA, there are two basic methods to use. First is to use column chromatography, many commercial columns are available (HPLC can also be used). The second is to use ethidium bromide in cesium chloride gradients. Ethidium bromide can fit between the stacked bases in D ...
Ch. 10 DNA Review Questions
... 9. True or False: Changes in chromatin structure and histone-DNA binding are associated with changes in gene activity. ____________ 10. What do nucleosomes do? ____________________________________________________ 11. What occurs during the process of replication? __________________________________ 1 ...
... 9. True or False: Changes in chromatin structure and histone-DNA binding are associated with changes in gene activity. ____________ 10. What do nucleosomes do? ____________________________________________________ 11. What occurs during the process of replication? __________________________________ 1 ...
Genetics and DNA Replication Notes
... Deletions, insertions and substitutions (see packet for illustrations and examples) Gene mutations involve a single base, or a few bases Substitution – “point mutation” One base changed Ex. ATA becomes AAA Insertion – base added Deletion – base is missing *“frameshift” = caused by insertion and dele ...
... Deletions, insertions and substitutions (see packet for illustrations and examples) Gene mutations involve a single base, or a few bases Substitution – “point mutation” One base changed Ex. ATA becomes AAA Insertion – base added Deletion – base is missing *“frameshift” = caused by insertion and dele ...
Molecular genetics
... site of translation. PolyA tailing is the process by which a long tail of Adenine residue is added to the 3’ end of m-RNA during splicing. Ribozymes are RNA molecules act as enzymes. RNase P is a Ribozyme. 9. Recombinant DNA technology involves manipulation of DNA which involves DNA cloning, DNA pro ...
... site of translation. PolyA tailing is the process by which a long tail of Adenine residue is added to the 3’ end of m-RNA during splicing. Ribozymes are RNA molecules act as enzymes. RNase P is a Ribozyme. 9. Recombinant DNA technology involves manipulation of DNA which involves DNA cloning, DNA pro ...
No Slide Title
... chromosome pairs are pulled away from each other towards opposite ends of the cell Anaphase II: sister chromatids are split apart at the centromere and move to the opposite pole. ...
... chromosome pairs are pulled away from each other towards opposite ends of the cell Anaphase II: sister chromatids are split apart at the centromere and move to the opposite pole. ...
Unit 4
... information – much like the letters of the alphabet. In DNA or RNA, the monomers are the four types of nucleotides, which differ in their nitrogenous bases. Genes are hundreds of thousands of nucleotides long – each gene with a different specific sequence. A protein also has monomers arranged in a p ...
... information – much like the letters of the alphabet. In DNA or RNA, the monomers are the four types of nucleotides, which differ in their nitrogenous bases. Genes are hundreds of thousands of nucleotides long – each gene with a different specific sequence. A protein also has monomers arranged in a p ...
Cre-Lox recombination
In the field of genetics, Cre-Lox recombination is known as a site-specific recombinase technology, and is widely used to carry out deletions, insertions, translocations and inversions at specific sites in the DNA of cells. It allows the DNA modification to be targeted to a specific cell type or be triggered by a specific external stimulus. It is implemented both in eukaryotic and prokaryotic systems.The system consists of a single enzyme, Cre recombinase, that recombines a pair of short target sequences called the Lox sequences. This system can be implemented without inserting any extra supporting proteins or sequences. The Cre enzyme and the original Lox site called the LoxP sequence are derived from bacteriophage P1.Placing Lox sequences appropriately allows genes to be activated, repressed, or exchanged for other genes. At a DNA level many types of manipulations can be carried out. The activity of the Cre enzyme can be controlled so that it is expressed in a particular cell type or triggered by an external stimulus like a chemical signal or a heat shock. These targeted DNA changes are useful in cell lineage tracing and when mutants are lethal if expressed globally.The Cre-Lox system is very similar in action and in usage to the FLP-FRT recombination system.