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Experimental Ecology
Experimental Ecology

... Environmental Gene Transfer • DNA can be tranferred between microbes in a number of different ways: via DNA uptake from dead cells (A), Plasmids (B) and phage infection (C) • Plasmids are circular, mobile genetic elements capable of replication and transfer from one bacterium to another ...
Introduction and review Lecture 1: Jan. 18, 2006
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... Genotype- The genetic constitution of an organism. Phenotype- The visible appearance of an organism. Homologous chromosomes- in a diploid organism, the 2 copies of a chromosome inherited from the mother and the father. Locus- Location of a gene on a chromosome. Allelomorph (allele)- different versio ...
Biology Honors Final Review
Biology Honors Final Review

... 3. What organelle regulates what gets into the cell? 4. Describe exocytosis and endocytosis. Why are these processes important to a cell? Unit: 5 1. What types of organisms use photosynthesis? 2. Where does photosynthesis occur? 3. What is the cell’s energy currency? 4. Why is photosynthesis importa ...
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... 1. What is the goal of population genetics? What forces drive changes in genetic diversity? 2. What is the value of gene trees? 3. What does a significant deviation from Tajima’s D imply with regards to the neutrality of the evolution of a gene? 4. Describe the relationship between diversity and rec ...
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... Proteases block building of Virus Proteins Integrases block integration of vDNA into host DNA  AZT prematurely ends viral DNA replication  Natural Immunities (CD-4 altered or super ...
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Cre-Lox recombination



In the field of genetics, Cre-Lox recombination is known as a site-specific recombinase technology, and is widely used to carry out deletions, insertions, translocations and inversions at specific sites in the DNA of cells. It allows the DNA modification to be targeted to a specific cell type or be triggered by a specific external stimulus. It is implemented both in eukaryotic and prokaryotic systems.The system consists of a single enzyme, Cre recombinase, that recombines a pair of short target sequences called the Lox sequences. This system can be implemented without inserting any extra supporting proteins or sequences. The Cre enzyme and the original Lox site called the LoxP sequence are derived from bacteriophage P1.Placing Lox sequences appropriately allows genes to be activated, repressed, or exchanged for other genes. At a DNA level many types of manipulations can be carried out. The activity of the Cre enzyme can be controlled so that it is expressed in a particular cell type or triggered by an external stimulus like a chemical signal or a heat shock. These targeted DNA changes are useful in cell lineage tracing and when mutants are lethal if expressed globally.The Cre-Lox system is very similar in action and in usage to the FLP-FRT recombination system.
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