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Build whatever you want - Hicksville Public Schools / Homepage
Build whatever you want - Hicksville Public Schools / Homepage

... protein. Therefore it is the genetic code: DNA base sequence that ultimately determine a protein’s sequence of amino acids. ...
Genetics of Viruses and Bacteria
Genetics of Viruses and Bacteria

... Conjugation • Direct transfer of genetic material (usually plasmid DNA) from two bacterial cells that are temporarily joined by a sex pili. • Plasmid genes are not required for survival, but they tend to code for genes that increase fitness (ex. antibiotic resistance) video ...
BINF 4445/5445
BINF 4445/5445

The Secret Code of Life: - Richmond School District
The Secret Code of Life: - Richmond School District

... the 4 nucleotides, A,C,G and T. Only 3 nucleotides form a triplet which, when in a gene, codes for a part of a protein. There are 34 total different triplets that can be created but only 20 different amino acids. (Would a doublet code work just as well?? i.e. only 2 nucleotides to represent 20 amino ...
Gene!
Gene!

... was originally produced by the action of proflavins. We@ have previously argued that acridines such aa pro5vin act as mutagens because they add or dslsts a base or bases. The most striking evidence in favour of this is that mutants produced by a&dines are seldom ‘leaky’ ; they are almost always comp ...
Key concepts_chromatin
Key concepts_chromatin

... Chromatin fibers tend to fold into a compact 30-nm fiber at physiological salt concentrations. The fine structure of the 30-nm fiber remains a matter or controversy. Individual chromosomes appear to inhabit unique territories in the interphase nucleus. In mitosis, sister chromatids condense into the ...
English - iGEM 2016
English - iGEM 2016

... What is synthetic biology? Modifying cells and bacteria, so they can fulfill certain tasks ...
Multiple Choice
Multiple Choice

... c. Proteins that bind to regulatory sites on DNA determine whether a gene is expressed. d. RNA polymerase regulates gene expression. ____13. A lac repressor turns off the lac genes by binding to a. the promoter. c. the operator. b. tRNA. d. the lac genes. ____14. Gene regulation in eukaryotes a. usu ...
Strawberry DNA Extraction Lab
Strawberry DNA Extraction Lab

... bilayers of the cell membrane and organelles. The salt is used to break up protein chains that bind around the nucleic acids. Finally, ethanol is added. DNA is soluble in water, but not ethanol. The colder the ethanol, the less soluble the DNA will be. The alcohol causes DNA to precipitate, or settl ...
Slides
Slides

... Complementary Base Pairing ...
Lab 12
Lab 12

... Humans: 46 chromosomes 23 homologus pairs (one of each from mom, one of each from dad) -2 copies of each gene, each is 1 allele -allele = a version of a gene -the 2 alleles could be the same or they could be different: same = homozygous (GG or gg) different = heterozygous (Gg) genotype = ones geneti ...
Bacterial plasmids
Bacterial plasmids

WELCOME TO BIOLOGY 2002 - University of Indianapolis
WELCOME TO BIOLOGY 2002 - University of Indianapolis

... A single strand of nucleotides is made when a phosphodiester bond is formed between the 3’ C of one nucleotide and the 5’ C of ...
Cloning and Sequencing
Cloning and Sequencing

... Background ...
Part II: Recombinant DNA Technology
Part II: Recombinant DNA Technology

... Restriction enzymes recognize a specific sequence of nucleotides and produce a double-stranded cut in the DNA. While recognition sequences vary between 4 and 8 nucleotides, many of them are palindromic, which correspond to nitrogenous base sequences that read the same backwards and forwards. In the ...
BAC vectors (Bacterial Artificial Chromosome)
BAC vectors (Bacterial Artificial Chromosome)

... not essential for viral growth are removed from the viral DNA and replaced with the DNA to be cloned. Up to ~25 kb of foreign DNA can be inserted into the λ genome, resulting in a recombinant DNA that can be packaged in vitro to form virions capable of replicating and forming plagues on E. coli host ...
Genes get around
Genes get around

...  Host cell DNA is chopped up, phage makes copies of its own DNA and proteins  New parts of virus assemble, cell lyses, viruses escape to infect other cells. ...
BACTERIA TRANSFORMATION LAB (ACTIVITY)
BACTERIA TRANSFORMATION LAB (ACTIVITY)

... are small, circular pieces DNA that can be exchanged naturally between bacteria. Plasmids may contain genes, and when these genes are expressed they can provide bacteria with special traits such as antibiotic resistance. Molecular biologists have developed procedures to take advantage of the natural ...
Chapter 14: DNA Technologies
Chapter 14: DNA Technologies

... (1) The Southern blot can be used to diagnose genetic disorders c) Blots used for RNA, separated by electrophoresis, are called Northern blots; the Western blot is used for protein or polypeptide molecules (One well known use is to detect antibodies, such as antibodies to HIV: the test for AIDS) G. ...
Notes
Notes

... DNA code to the ribosome to direct the making of protein; occurs in nucleus • Translation - the process of building proteins, the sequence of bases of mRNA is “translated” into a sequence of amino acids; occurs in ribosome ...
EOC PRACTICE QUESTIONS #2
EOC PRACTICE QUESTIONS #2

... 109. Genetic disorder characterized by abnormal shape of red blood cell that make them unable to carry oxygen is ______. People who are heterozygous are immune to the mosquito carrying disease called ______________. ...
DNA to Eye Color? Just How does it Happen?
DNA to Eye Color? Just How does it Happen?

... long sequences of bases • 30,000 genes in humans –3 billion base pairs • Base pairs make up code for amino acid sequence, which ...
GENETIC TERMINOLOGY
GENETIC TERMINOLOGY

... (frequency ÷ total class) ...
E. coli HST08 Premium Electro
E. coli HST08 Premium Electro

Unit 3 Biotechnology
Unit 3 Biotechnology

... • Genes: small sections of DNA responsible for traits – Chromosomes: rod-like structures – Occur in pairs of linked strands (twisted ladder) – Bases: chemicals that connect strands—adenine (A), guanine (G), cytosine (C), and thymine (T) – Order of bases between the strands: controls genetic traits ...
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Cre-Lox recombination



In the field of genetics, Cre-Lox recombination is known as a site-specific recombinase technology, and is widely used to carry out deletions, insertions, translocations and inversions at specific sites in the DNA of cells. It allows the DNA modification to be targeted to a specific cell type or be triggered by a specific external stimulus. It is implemented both in eukaryotic and prokaryotic systems.The system consists of a single enzyme, Cre recombinase, that recombines a pair of short target sequences called the Lox sequences. This system can be implemented without inserting any extra supporting proteins or sequences. The Cre enzyme and the original Lox site called the LoxP sequence are derived from bacteriophage P1.Placing Lox sequences appropriately allows genes to be activated, repressed, or exchanged for other genes. At a DNA level many types of manipulations can be carried out. The activity of the Cre enzyme can be controlled so that it is expressed in a particular cell type or triggered by an external stimulus like a chemical signal or a heat shock. These targeted DNA changes are useful in cell lineage tracing and when mutants are lethal if expressed globally.The Cre-Lox system is very similar in action and in usage to the FLP-FRT recombination system.
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