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The Chromosome
The Chromosome

...  Involves exchange between much smaller regions of DNA sequence (approximately 20 - 200 base pairs) and requires the recognition of specific sequences by the proteins involved in the recombination process.  Site-specific recombination events occur primarily as a mechanism to alter the program of g ...
What is DNA Fingerprinting
What is DNA Fingerprinting

... 2. Go to the following site: http://www.pbs.org/wgbh/nova/sheppard/analyze.html Before doing the activity, read the following background information: Background Essay: Create a DNA Fingerprint In the last 15 years, DNA has played an increasingly important role in our legal system. Tissue evidence is ...
Wednesday, September 5
Wednesday, September 5

... Some human genes are too large to be incorporated into bacterial plasmids. Bacterial cells lack the means to process RNA transcripts, and even if the need for RNA processing is avoided by using cDNA, bacteria lack enzymes to catalyze the post-translational processing that many human proteins undergo ...
DNA Transcription / Translation
DNA Transcription / Translation

... Which of the following does not affect the final outcome of gene expression? A. the environment of the cells  B. the number of amino acids in the protein ...
What is Genetic Engineering?
What is Genetic Engineering?

... ancestral teosinte (left). ...
1-3 - PLOS
1-3 - PLOS

... is then linearized by double restriction at the Selection site. The linearized double stranded device is then subjected to heat renaturation, randomly annealing single strands from different devices in the library and exposing the mismatched bases between their input modules. As discussed earlier, o ...
Document
Document

... Sickle cell anaemia is caused due to substitution of: (1) Valine at 6th position of alpha globin chain by glutamic acid. (2) Valine at 6th position of beta globin chain by glutamin. (3) Glutamic acid at the 6th position of beta globin chain by valine. (4) Glycine at the 6th position of alpha globin ...
Recombinant DNA and Genetic Engineering
Recombinant DNA and Genetic Engineering

... genetics of other species for thousands ...
Central Dogma WebQuest - Life Science
Central Dogma WebQuest - Life Science

... Answer each of the questions as you travel to the webpages below. Links can be found here: mvhslifescience.weebly.com → Biology → DNA → WebQuest (bottom of the page) From Gene to Protein: Transcription Complete the tutorial by clicking “Next Concept” and reading each page. Answer the questions and f ...
Bio 309F
Bio 309F

... 19. The size of a gene is defined by A. start codon B. stop codon C. Introns D. A and B E. A, B, and C are correct 20. Biological functions of proteins/polypeptides (mechanism whereby the genetic information of a gene is carried out by proteins) are determined by A. number of amino acids B. ratio of ...
Glossary of Terms – Molecular Biology, Genetics, Clinical Neurology
Glossary of Terms – Molecular Biology, Genetics, Clinical Neurology

... Chromosomes: The self-copying genetic structures of cells containing the cellular DNA that bears in its nucleotide sequence the genes or blueprints for cell function. In prokaryotes (cells that do not have a nucleus), chromosomal DNA is circular, and the entire genome (all the genes in an organism) ...
NAME Date DNA Structure Review Figure 1 The untwisted form of
NAME Date DNA Structure Review Figure 1 The untwisted form of

... there is a relationship between DNA and _________________________. 20. Only the bases, which form the steps of the DNA ladder, control inheritance. There are thousands of genes in any one organism, such as a human being. Since there are only four bases, then one base ______________________ (could / ...
Café DNA - www .alexandria .k12 .mn .us
Café DNA - www .alexandria .k12 .mn .us

... The job of DNA is to give information for protein synthesis to the mRNA. The DNA resides in the cell’s nucleus – in this case, the hallway. The DNA is to look carefully at the blueprint (model) for their group’s protein. They may NOT show the model to anyone else in their group! When visited by the ...
Homework Assignment #7
Homework Assignment #7

... identify how they relate to each other but also how they are distinct. 1a) What are HbA and HbS and how do they differ from each other? Focus on the molecules! (10 Points) ...
DNA Technology
DNA Technology

...  Problems due to differences in how prokaryotic & ...
DNA and RNA are nucleic acids that carry out cellular
DNA and RNA are nucleic acids that carry out cellular

... In prokaryotes, the DNA is not enclosed in a membranous envelope, but rather free-floating within the cytoplasm. The entire genetic content of a cell is known as its genome and the study of genomes is genomics. In eukaryotic cells, but not in prokaryotes, DNA forms a complex with histone proteins to ...
The polymerase chain reaction (PCR)
The polymerase chain reaction (PCR)

... commence. The heating cycle is complicated, but includes temperatures briefly ranging almost all the way to the boiling point, so durability in the polymerase is essential. The basic steps of the polymerase chain reaction are as follows. All the ingredients are mixed together in a small vial, usuall ...
G3: Genes, Genomes and Genetics Whole organism genome
G3: Genes, Genomes and Genetics Whole organism genome

... chromosomes, mutations or deletions that are usually not available in most organisms. With ObLiGaRe, the integration efficiency into the germline genome was high enough (7%) for us to recover eight antibiotic resistant individual lines (from different G0 males) out of 116 successful G0 crosses. More ...
Lect-7
Lect-7

... cause mutations. It is known to cause mutations at high rate.  The mutations proved to be insertions to Mu at random sites in the host genome disrupting the functioning of different genes. ...
DNA and RNA are nucleic acids that carry out cellular
DNA and RNA are nucleic acids that carry out cellular

... DNA and RNA A nucleotide is made up of three components: a nitrogenous base, a pentose sugar, and one or more phosphate groups. Carbon residues in the pentose are numbered 1′ through 5′ (the prime distinguishes these residues from those in the base, which are numbered without using a prime notation ...
Document
Document

... Structure Determines Function When genes are changed, the proteins they code for may change and this can affect cell structure and function,which changes a phenotype. ...
Genomes 1
Genomes 1

... recombinant DNA, there are small gaps or nicks in the sugar phosphate backbone.  These are sealed by DNA ligase: ...
Biochemistry ± DNA Chemistry and Analysis DNA o Adenosine
Biochemistry ± DNA Chemistry and Analysis DNA o Adenosine

... ƒ Endonuclease: eat the interior RIDSRO\QXFOHRWLGHVSHFLILFIRU¶RU¶VLGHRISKRVphodiester bond x Can cut ssDNA or dsDNA depending on nuclease o Restriction Endonuclease: recog specific base sequence in foreign DNA for degradation & cleave both strands ƒ Found in bacteria and not in eukaryot ...
Protein Synthesis Notes
Protein Synthesis Notes

... Gene mutations: a change in the sequence of nucleotides within a gene. When do mutations occur? - most often during DNA replication. ...
mutation PP
mutation PP

... • The information carried by mRNA is translated into a sequence of amino acids. This requires a ribosome (containing rRNA) and tRNA to bring the correct amino acids to the mRNA ...
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Cre-Lox recombination



In the field of genetics, Cre-Lox recombination is known as a site-specific recombinase technology, and is widely used to carry out deletions, insertions, translocations and inversions at specific sites in the DNA of cells. It allows the DNA modification to be targeted to a specific cell type or be triggered by a specific external stimulus. It is implemented both in eukaryotic and prokaryotic systems.The system consists of a single enzyme, Cre recombinase, that recombines a pair of short target sequences called the Lox sequences. This system can be implemented without inserting any extra supporting proteins or sequences. The Cre enzyme and the original Lox site called the LoxP sequence are derived from bacteriophage P1.Placing Lox sequences appropriately allows genes to be activated, repressed, or exchanged for other genes. At a DNA level many types of manipulations can be carried out. The activity of the Cre enzyme can be controlled so that it is expressed in a particular cell type or triggered by an external stimulus like a chemical signal or a heat shock. These targeted DNA changes are useful in cell lineage tracing and when mutants are lethal if expressed globally.The Cre-Lox system is very similar in action and in usage to the FLP-FRT recombination system.
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