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PCRBIO Taq DNA Polymerase
PCRBIO Taq DNA Polymerase

... specificity. The enzyme and buffer system allow for superior PCR performance on complex templates such as mammalian genomic DNA. PCRBIO Taq DNA Polymerase is a robust enzyme for all your everyday PCR applications including genotyping, screening and library construction. PCRBIO Taq DNA Polymerase per ...
Learning outcomes and competences
Learning outcomes and competences

... The Danish and global plant production is facing major challenges. The rapid population growth, the increased consumption of animal products and the use of crops for energy purposes demand an increased production. In addition, climate change is expected to have pronounced effects on plant production ...
DNA
DNA

... - In eukaryotes, DNA does not exist in isolation but rather it is wound/wrapped (or negatively supercoiled) around bead-like protein complexes called “histones” to form what has come to be known as a “nucleosome” - Nucleosomes represent the first building blocks for packaging/folding DNA into a high ...
exam 2 summary
exam 2 summary

... >Pcr can be used to amplify short polymorphisms. The procedures for all >three detection systems are similar. The PCR product is loaded into a gel >but this gel is made out of a different substance called polyacrylamide. >This gel is more appropriate for analyzing the small size PCR products. The >f ...
EOC 3
EOC 3

... Framingham Study that smoking by itself increases the risk of heart disease. Which of the following types of scientific investigation would provide the best evidence against this scientist’s genetic explanation of the link between smoking and heart disease? A. Interviews with smokers indicating that ...
Transcription, RNA Processing, and
Transcription, RNA Processing, and

... Most bacteria possess several different sigma factors, each of which mediate transcription from several hundred genes … ...
"Preparation of Genomic DNA from Bacteria". In: Current Protocols in
"Preparation of Genomic DNA from Bacteria". In: Current Protocols in

Translation Details
Translation Details

... 3) How many nucleotides make a codon? 4) The anticodon can be found on which object? 5) The anticodon must match the ______. 6) Place the following steps in order from start to finish: a. Proteins are assembled b. Ribosome reads a codon one at a time c. mRNA arrives at the ribosome d. tRNA delivers ...
Level 2 Biology (90464) 2011
Level 2 Biology (90464) 2011

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Other genomic arrays: Methylation, chIP on chip…

Crystal structure of Cas9 in complex with guide RNA and target DNA
Crystal structure of Cas9 in complex with guide RNA and target DNA

... [3], the Cas9–sgRNA system has been attracting much attention as a new, versatile genome-editing technology, which works effectively in various types of cells and organisms. Catalytically dead or inactive Cas9 (referred to as dCas9) can serve as an RNAguided genome-targeting platform, and dCas9-base ...
Comparative Genomics IV
Comparative Genomics IV

... The X and Y chromosomes of human and other mammalian species are heteromorphic. The human X chromosome is a submetacentric chromosome which contains about 165 Mb of DNA, whereas the Y chromosome is acrocentric and is much smaller – about 60 Mb of DNA. The human X chromosome contains numerous importa ...
Gene Expression and Regulation
Gene Expression and Regulation

... changing conditions. For eukaryotes, gene regulation is tied to maintaining homeostasis – a consistent internal environment in the face of ever-changing external conditions. Multicellular organisms require different genes at different times of growth and development in different tissues. We have mor ...
PlayMais 3-D DNA Model
PlayMais 3-D DNA Model

DNA repair - Journal of Cell Science
DNA repair - Journal of Cell Science

... global genome repair (GGR), which removes damage in the genome overall and transcription-coupled repair (TCR), which specifically repairs the transcribed strand of active genes. The main difference between GGR and TCR is the requirement for different factors during the initial recognition steps. UV- ...
Cha. 3 Cell structure
Cha. 3 Cell structure

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Barth Syndrome Service at BGL

Transformation
Transformation

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... Modularity as an Organizing Principle in Protein Structure ...
5.2. Protocol for PCR
5.2. Protocol for PCR

... passing the ER membrane through the protein translocation complex, the nascent polypeptide encounters multiple aspects that facilitate the protein to achieve its native conformation. The ER harbors an oxidative environment and a diverse set of folding factors which together enhance the rate in whic ...
The ocean as regional enabler A Pacific Northwest success
The ocean as regional enabler A Pacific Northwest success

... present in the samples. • Second, low-cost biosensors to assay the selected analytes in time series across chemical and physical gradients in different habitats. Like planes and helicopters: both are used to fly, but have very different applications. ...
molecular biology
molecular biology

... discovery of DNA structure by Watson and Crick in 1952 saw fast growth in our understanding of the biological processes at the molecular level. As the basics of various processes became clear and details of their regulation were being revealed, the molecular biologist started visualizing a scenario ...
Pupko_pairwise
Pupko_pairwise

... Query sequence: DNA or protein? • For coding sequences, we can use the DNA sequence or the protein sequence to search for similar sequences. • Which is preferable? ...
Molecular cloning and tissue distribution of the Toll
Molecular cloning and tissue distribution of the Toll

... and some of their functions in shrimp innate immunity against foreign molecules have been determined. The objective of the present study was to clone the full-length Toll receptor from P. monodon, PmToll, to compare its sequence with other invertebrate organisms, and to study its tissue distribution ...
Successive Losses of Central Immune Genes
Successive Losses of Central Immune Genes

... depleted of fish (Rogers 2000; Priede and Froese 2013). This is supported by higher extinction rates in the same period (Takashima et al. 2006; Harnik et al. 2012). The anoxic scenario fits with one of several mechanisms proposed to promote loss of MHCII—metabolic cost (Star and Jentoft 2012). Never ...
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Molecular evolution

Molecular evolution is a change in the sequence composition of cellular molecules such as DNA, RNA, and proteins across generations. The field of molecular evolution uses principles of evolutionary biology and population genetics to explain patterns in these changes. Major topics in molecular evolution concern the rates and impacts of single nucleotide changes, neutral evolution vs. natural selection, origins of new genes, the genetic nature of complex traits, the genetic basis of speciation, evolution of development, and ways that evolutionary forces influence genomic and phenotypic changes.
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