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103 Lecture Ch22a
103 Lecture Ch22a

... synthesized, the ribosome reaches the the “stop” codon: UGA, UAA, or UAG • There is no tRNA with an anticodon for the “stop” codons • Therefore, protein synthesis ends (termination) • The polypeptide is released from the ribosome and the protein can take on it’s 3-D structure (some proteins begin fo ...
Ribonucleic acids are found in both the nucleus and the cytoplasm
Ribonucleic acids are found in both the nucleus and the cytoplasm

... work by Chargaff and other workers led Crick and Watson to propose that the three dimensional structure of DNA consisted of two single molecule polymer chains held together in the form of a double helix by hydrogen bonding between the same pairs of bases, namely the adenine–thymine and cytosine–guan ...
From Gene to Protein
From Gene to Protein

... tRNA= carries a specific amino acid to ribosome based on its anticodon to mRNA codon rRNA= makes up 60% of the ribosome; site of protein synthesis snRNA=small nuclear RNA; part of a spliceosome. Has structural and catalytic roles srpRNA=a signal recognition particle that binds to signal peptides RNA ...
CRISPR/Cas9.
CRISPR/Cas9.

... homology to the regions flanking the DSB, leading to incorporation of the template sequence into the genome [4]. 5. What applications is this technique employed for? CRISPR/Cas9 is used as a genome editing tool for a variety of purposes in research. The technique is less expensive and far more preci ...
From DNA To Protein
From DNA To Protein

... • Bases in DNA and bases in mRNA strands are same in all organisms • Only sequence of bases differs • Each codon of mRNA “codes” for a particular amino acid or a stop codon • Genetic code can be translated to determine amino acid sequence for proteins from any section of DNA • Since all organisms us ...
Schematic courtesy of B. Crump Quantitative (Real Time) PCR
Schematic courtesy of B. Crump Quantitative (Real Time) PCR

... •Very few genomes reconstructed •Sequenced 6.3 billion DNA base pairs (Human genome is ~3.2) from top 5 m of ocean •Discovered more than 6 million genes… and they are only halfway done! ...
Some transcription factors ("Enhancer
Some transcription factors ("Enhancer

... particular cell type. Specifically, the promoter dictates where transcription starts and is located just upstream of that site. Enhancers influence the level of transcription as well as the cell-type specificity. Enhancers can be located at varying distances and upstream, within, or downstream, of t ...
DNA and Genes
DNA and Genes

... Everyone acquires some changes to their DNA during the course of their lives. I These changes occur in a number of ways. Sometimes there are simple copying errors that are introduced when DNA replicates itself. (Every time a cell divides, all of its DNA is duplicated so that the each of the two resu ...
Big Idea 3
Big Idea 3

... simple and elegant solution for the transmission of heritable information to the next generation; by using each strand as a template, existing information can be preserved and duplicated with high fidelity within the replication process. However, the process of replication is imperfect, and errors o ...
Notes with questions
Notes with questions

Nucleic Acids - Somma Science
Nucleic Acids - Somma Science

... DNA is also known as the hereditary material or genetic information. It is found in genes, and its sequence of bases makes up a code. Between "starts" and "stops," the code carries instructions for the correct sequence of amino acids in a protein (see Figure 1.3). DNA and RNA have different function ...
DNA Technology
DNA Technology

... • __________________________ is the manipulation of DNA for practical purposes such as: o ________________________________ using DNA fingerprinting o _______________________________________ o Identifying ______________________________________ before symptoms appear o _________________________ for cu ...
Name: Protein Synthesis PRICE DNA DNA contains ______
Name: Protein Synthesis PRICE DNA DNA contains ______

... • Copies DNA & leaves through __________ pores • Contains the Nitrogen Bases A, G, C, ____ ( no T ) • Carries the information for a ___________ protein • Made up of 500 to 1000 nucleotides long • Sequence of ____ bases called codon • AUG – methionine or start ________ • UAA, UAG, or UGA – ______ cod ...
CHAPTER 4, PART 2
CHAPTER 4, PART 2

... A. A codon (3 bases) specifies an amino acid B. Sequential and non-overlapping C. Degenerate (more than one codon/amino acid) D. Some codons are start and stop signals E. The code is nearly universal (see differences in human mitochondrial code) F. Sequences of bases in genes and amino acids in thei ...
protein synthesis
protein synthesis

... • single stranded • sugar = ribose • bases = AUCG • carries code for making proteins from DNA in nucleus to ribosomes in cytoplasm 2 subunits small large ...
A land plant-specific multigene family in the unicellular
A land plant-specific multigene family in the unicellular

... (which suggests that multiple coding regions are also present in these lineages). The large number of functional gene copies and the overall level of divergence among both Mesostigma and land plant BIP2-like sequences (supplementary figs. 1 and 2 [fig.S1_pdf and fig_S2.pdf] in Supplementary Material ...
BDOL Interactive Chalkboard
BDOL Interactive Chalkboard

... The Genetic Code • The nucleotide sequence transcribed from DNA to a strand of messenger RNA acts as a genetic message, the complete information for the building of a protein. • As you know, proteins contain chains of amino acids. You could say that the language of proteins uses an alphabet of amin ...
From Gene to Protein
From Gene to Protein

... tRNA= carries a specific amino acid to ribosome based on its anticodon to mRNA codon rRNA= makes up 60% of the ribosome; site of protein synthesis snRNA=small nuclear RNA; part of a spliceosome. Has structural and catalytic roles srpRNA=a signal recognition particle that binds to signal peptides RNA ...
Key Area 2 – Pupil Booklet
Key Area 2 – Pupil Booklet

... 7. give the opposite bases in a genetic code with the letters A,T C and G 8. explain that by testing a DNA sample, this information can be used to identify a person’s father, or can be used to link people to a crime scene. 10. state that testing for a particular gene can identify a genetic disorder. ...
PRACTICE EXAM ANSWERS 2007 1. A. Essentially
PRACTICE EXAM ANSWERS 2007 1. A. Essentially

... 3. Drug testing / looking for side effects and/or drug specificity C. KO animals are more technically demanding. The procedure is more complicated, and success relies on achieving and detecting a very rare event – targeted homologous recombination as well as germ line transmission. D. The overexpres ...
HL-SAN for DNA removal in protein purification - A4
HL-SAN for DNA removal in protein purification - A4

... HL-SAN for DNA removal in protein purification Nucleic acids, and especially genomic DNA, often pose a problem in purification of DNA-binding proteins as they interfere with purification, downstream analysis or applications. Nucleases activity is usually difficult to remove while HL-SAN is easily in ...
Ligation and Transformation
Ligation and Transformation

... • Endonucleases: – in nature, they protect bacteria from intruding DNA – cut up (restrict) the viral DNA – cut only at very specific nucleotide sequences • Restriction site: recognition sequence for a particular restriction enzyme • Restriction fragments: segments of DNA cut by restriction enzymes i ...
03 Nucleic Acids
03 Nucleic Acids

... DNA is also known as the hereditary material or genetic information. It is found in genes, and its sequence of bases makes up a code. Between "starts" and "stops," the code carries instructions for the correct sequence of amino acids in a protein (see Figure 1.3). DNA and RNA have different function ...
DNA, and in some cases RNA, is the primary source of heritable
DNA, and in some cases RNA, is the primary source of heritable

... transcript. By selective removing different parts of an RNA transcript, different mRNA's can be produced, each coding for a different protein product. Thus the number of different proteins an organism can produce is much greater than its number of genes. Check out the mRNA processing activity in you ...
No Slide Title
No Slide Title

... Pease et al (1994) Proc. Natl. Acad. Sci. U.S.A. 91, 5022. ...
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Molecular evolution

Molecular evolution is a change in the sequence composition of cellular molecules such as DNA, RNA, and proteins across generations. The field of molecular evolution uses principles of evolutionary biology and population genetics to explain patterns in these changes. Major topics in molecular evolution concern the rates and impacts of single nucleotide changes, neutral evolution vs. natural selection, origins of new genes, the genetic nature of complex traits, the genetic basis of speciation, evolution of development, and ways that evolutionary forces influence genomic and phenotypic changes.
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