The Basics of RT-PCR
... than that obtained from cDNA. (Note: since introns can be large, frequently ⬎ 1 kb, intron-spanning primers frequently do not amplify the larger genomic sequence efficiently, and no product is obtained from genomic DNA). In the early days of PCR, primers were often selected on a fairly random basis ...
... than that obtained from cDNA. (Note: since introns can be large, frequently ⬎ 1 kb, intron-spanning primers frequently do not amplify the larger genomic sequence efficiently, and no product is obtained from genomic DNA). In the early days of PCR, primers were often selected on a fairly random basis ...
Biomolecular chemistry 3. Translating the genetic code
... harmful and sometimes beneficial). Thus, degeneracy minimizes the deleterious effects of mutations. • Degeneracy of the code may also be significant in permitting DNA base composition to vary over a wide range without altering the amino acid sequence of the proteins encoded by the DNA. The G + C con ...
... harmful and sometimes beneficial). Thus, degeneracy minimizes the deleterious effects of mutations. • Degeneracy of the code may also be significant in permitting DNA base composition to vary over a wide range without altering the amino acid sequence of the proteins encoded by the DNA. The G + C con ...
Metagenomic analysis of a soil enrichment cultures
... 16s rRNA gene of virtually all species because they hybridize to a highly conserved region. However, an important difference here is that the template is a pool of mixed genomic DNA, so there are potentially 1000s of different 16s rRNA genes present. Each one is amplified in the reaction, yielding a ...
... 16s rRNA gene of virtually all species because they hybridize to a highly conserved region. However, an important difference here is that the template is a pool of mixed genomic DNA, so there are potentially 1000s of different 16s rRNA genes present. Each one is amplified in the reaction, yielding a ...
Introduction to 3D-Structure Visualization and Homology Modeling
... An InterPro family is a group of evolutionarily related proteins that share similar domain (or repeat) architecture. 2. Domain: An InterPro domain is an independent structural unit, which can be found alone or in conjunction with other domains or repeats. Domains are evolutionarily related. 3. Repea ...
... An InterPro family is a group of evolutionarily related proteins that share similar domain (or repeat) architecture. 2. Domain: An InterPro domain is an independent structural unit, which can be found alone or in conjunction with other domains or repeats. Domains are evolutionarily related. 3. Repea ...
REAL-TIME PCR
... agent, SYBR green. Although ethidium bromide was the first dye to be used as a DNA-binding fluorophore (Higuchi et al., 1992), SYBR green is the preferred dye because its binding affinity to doublestranded DNA (dsDNA) is more than 100 times higher than that of ethidium bromide and it does not interf ...
... agent, SYBR green. Although ethidium bromide was the first dye to be used as a DNA-binding fluorophore (Higuchi et al., 1992), SYBR green is the preferred dye because its binding affinity to doublestranded DNA (dsDNA) is more than 100 times higher than that of ethidium bromide and it does not interf ...
Brooker Chapter 15
... They can respond to changes in nutrient availability They can respond to environmental stresses ...
... They can respond to changes in nutrient availability They can respond to environmental stresses ...
PCR Optimization: Reaction Conditions and
... The PCR sample may be single- or double-stranded DNA or RNA. If the starting sample is RNA, total RNA, poly (A+) RNA, viral RNA or rRNA can be used. A reverse transcriptase (for example MuLV or rTth DNA Polymerase) is used to prepare first-strand cDNA prior to conventional PCR amplification. For RNA ...
... The PCR sample may be single- or double-stranded DNA or RNA. If the starting sample is RNA, total RNA, poly (A+) RNA, viral RNA or rRNA can be used. A reverse transcriptase (for example MuLV or rTth DNA Polymerase) is used to prepare first-strand cDNA prior to conventional PCR amplification. For RNA ...
Unit IX: Identification of a Gram
... and sodium citrate are included in them. For Salmonella typhi, bismuth sulfite agar, or SalmonellaShigella (SS) agar, appears to be the best media. Colonies of S. typhi on these media appear black due to the reduction of sulfite to sulfide. Other widely used media are ENDO agar, MacConkey agar, Hekt ...
... and sodium citrate are included in them. For Salmonella typhi, bismuth sulfite agar, or SalmonellaShigella (SS) agar, appears to be the best media. Colonies of S. typhi on these media appear black due to the reduction of sulfite to sulfide. Other widely used media are ENDO agar, MacConkey agar, Hekt ...
Capillary Electrophoresis of Oligonucleotides
... Electrophoresis, operationally defined as the migration of analytes within an electrolyte solution under the influence of an electrical field, was first described by Tiselius in the 1930s [1]. Since its introduction, electrophoresis has become a primary tool of biochemical separation and has spawned ...
... Electrophoresis, operationally defined as the migration of analytes within an electrolyte solution under the influence of an electrical field, was first described by Tiselius in the 1930s [1]. Since its introduction, electrophoresis has become a primary tool of biochemical separation and has spawned ...
simultaneous detection of four food borne bacterial pathogens by
... Conventional methods of the detection of bacterial pathogens in foods generally based on the identification of bacteria using selective culture media by their morphological, biochemical and immunological characteristics (Wang et al. 2007). These methods are tedious, time consuming, and possible to h ...
... Conventional methods of the detection of bacterial pathogens in foods generally based on the identification of bacteria using selective culture media by their morphological, biochemical and immunological characteristics (Wang et al. 2007). These methods are tedious, time consuming, and possible to h ...
Popped Secret: The Mysterious Origin of Corn HHMI BioInteractive
... 3. Dr. Doebley and his team compared the DNA sequence of maize to that of a number of teosinte varieties from throughout Mexico. What did their analysis reveal? Select all that apply. A. That teosinte and maize have the same number of chromosomes. B. That maize originated from a variety of teosinte ...
... 3. Dr. Doebley and his team compared the DNA sequence of maize to that of a number of teosinte varieties from throughout Mexico. What did their analysis reveal? Select all that apply. A. That teosinte and maize have the same number of chromosomes. B. That maize originated from a variety of teosinte ...
Genetics Class- Ch. 10 Notes
... Transcription Factors • In bacteria, operons control gene expression • In more complex organisms transcription factors control gene expression and link genome to environment • Over 2,000 • Mutations in transcription factors may cause a wide range of effects ...
... Transcription Factors • In bacteria, operons control gene expression • In more complex organisms transcription factors control gene expression and link genome to environment • Over 2,000 • Mutations in transcription factors may cause a wide range of effects ...
PINK1 positively regulates HDAC3 to suppress p53
... suppressing p53-dependent apoptosis has been recently emphasized; however, the molecular basis of modulation of p53 function by HDAC3 remains unclear. Here, we show that cytoplasmic PTEN-induced putative kinase 1 (PINK1) directly binds to and phosphorylates HDAC3 at Ser-424 to enhance its histone de ...
... suppressing p53-dependent apoptosis has been recently emphasized; however, the molecular basis of modulation of p53 function by HDAC3 remains unclear. Here, we show that cytoplasmic PTEN-induced putative kinase 1 (PINK1) directly binds to and phosphorylates HDAC3 at Ser-424 to enhance its histone de ...
Evolution of hard proteins in the sauropsid integument in relation to
... Hard skin appendages in amniotes comprise scales, feathers and hairs. The cell organization of these appendages probably derived from the localization of specialized areas of dermal–epidermal interaction in the integument. The horny scales and the other derivatives were formed from large areas of de ...
... Hard skin appendages in amniotes comprise scales, feathers and hairs. The cell organization of these appendages probably derived from the localization of specialized areas of dermal–epidermal interaction in the integument. The horny scales and the other derivatives were formed from large areas of de ...
060506-Meeting
... plasmids • Conjugative plasmids are very large, from 60k – 100k basepairs long •The TraJ protein is a regulatory protein responsible for initiating the DNA transfer cascade •DNA transfer during conjugation always begins at a specific sequence on the plasmid, OriT, the Origin of Transfer. ...
... plasmids • Conjugative plasmids are very large, from 60k – 100k basepairs long •The TraJ protein is a regulatory protein responsible for initiating the DNA transfer cascade •DNA transfer during conjugation always begins at a specific sequence on the plasmid, OriT, the Origin of Transfer. ...
