Solid Tumour Section Carcinoma with t(15;19) translocation Atlas of Genetics and Cytogenetics
... The BRD4-NUT fusion protein is composed of the Nterminal of BRD4 (amino acids 1-720 out of 1372) and almost the entire protein sequence of NUT (amino acids 6-1127). The N-terminal of BRD4 includes bromodomains 1 and 2 and other, less well characterized functional domains. Oncogenesis It has been sug ...
... The BRD4-NUT fusion protein is composed of the Nterminal of BRD4 (amino acids 1-720 out of 1372) and almost the entire protein sequence of NUT (amino acids 6-1127). The N-terminal of BRD4 includes bromodomains 1 and 2 and other, less well characterized functional domains. Oncogenesis It has been sug ...
Protocol
... a. gel electrophoresis to analyze the RT-PCR products Part I: Oocyte RNA with Stratagene’s “Absolutely RNA Miniprep Kit” The objective of this part of the experiment is to isolate high quality RNA, which means RNA that is not degraded and is free from DNA contamination. The Stratagene kit uses a spi ...
... a. gel electrophoresis to analyze the RT-PCR products Part I: Oocyte RNA with Stratagene’s “Absolutely RNA Miniprep Kit” The objective of this part of the experiment is to isolate high quality RNA, which means RNA that is not degraded and is free from DNA contamination. The Stratagene kit uses a spi ...
article in press
... Therefore, it seems more and more certain that these 98% participate in the coding of the structure of the human body and contain genetic information encoded in a way that we are still unable to decipher. The model of genetic regulation based on genes and DNA regulatory proteins being insufficient fo ...
... Therefore, it seems more and more certain that these 98% participate in the coding of the structure of the human body and contain genetic information encoded in a way that we are still unable to decipher. The model of genetic regulation based on genes and DNA regulatory proteins being insufficient fo ...
Dali: A Protein Structural Comparison Algorithm
... • 1D sequence comparisons has traditionally been (and still is) used to determine degree of relatedness, although a low degree of sequence homology may yield surprisingly similar structures. • 3D structural alignment is aimed at providing more information about the structure-function similarities be ...
... • 1D sequence comparisons has traditionally been (and still is) used to determine degree of relatedness, although a low degree of sequence homology may yield surprisingly similar structures. • 3D structural alignment is aimed at providing more information about the structure-function similarities be ...
Protein Modification, targeting and degradation Protein modification
... Ran helps move importins and exportins and their cargo in and out of the nucleus • RanGTP enhances binding between an exportin and its cargo but stimulates release of importin's cargo; RanGDT has the opposite effect, namely, it stimulates the release of exportin's cargo, but enhances the binding be ...
... Ran helps move importins and exportins and their cargo in and out of the nucleus • RanGTP enhances binding between an exportin and its cargo but stimulates release of importin's cargo; RanGDT has the opposite effect, namely, it stimulates the release of exportin's cargo, but enhances the binding be ...
Restriction fragment length polymorphism
... Polymerase chain reaction (PCR) • The polymerase chain reaction is an extremely versatile technique for copying DNA. • PCR allows a single DNA sequence to be copied (millions of times), or altered in predetermined ways. • PCR has many variations, like reverse transcription PCR (RT-PCR) for amplific ...
... Polymerase chain reaction (PCR) • The polymerase chain reaction is an extremely versatile technique for copying DNA. • PCR allows a single DNA sequence to be copied (millions of times), or altered in predetermined ways. • PCR has many variations, like reverse transcription PCR (RT-PCR) for amplific ...
BiochemLecture07
... • RanGTP enhances binding between an exportin and its cargo but stimulates release of importin's cargo; RanGDT has the opposite effect, namely, it stimulates the release of exportin's cargo, but enhances the binding between an importin and its cargo. Therefore, the exportin and its cargo may move t ...
... • RanGTP enhances binding between an exportin and its cargo but stimulates release of importin's cargo; RanGDT has the opposite effect, namely, it stimulates the release of exportin's cargo, but enhances the binding between an importin and its cargo. Therefore, the exportin and its cargo may move t ...
2013 - Barley World
... b. the telomere is reached. c. a specific sequence in the DNA template is reached that serves as a signal for termination. d. the polymerase encounters the promoter. 12. mRNA processing in eukaryotes refers to a. 3’ caps, 5’ tails, and exon removal. b. 5’ caps, 3’ tails, and intron removal. c. trans ...
... b. the telomere is reached. c. a specific sequence in the DNA template is reached that serves as a signal for termination. d. the polymerase encounters the promoter. 12. mRNA processing in eukaryotes refers to a. 3’ caps, 5’ tails, and exon removal. b. 5’ caps, 3’ tails, and intron removal. c. trans ...
Ashley, CT, Wilkinson, KD, Reines, D and Warren, ST: FMR1 protein: Conserved RNP family domains and selective RNA binding. Science 262:563-566 (1993).
... repetitive domains in the yeast protein HX and 14 domains of the chicken genevigillin (VIG) (10). Alignments of these amino acid sequencesand a resulting profile search revealed a number of proteins containing 1 to 14 repeats of an uninterrupted, 30amino acid domain (Fig. 1A). Proteins containing th ...
... repetitive domains in the yeast protein HX and 14 domains of the chicken genevigillin (VIG) (10). Alignments of these amino acid sequencesand a resulting profile search revealed a number of proteins containing 1 to 14 repeats of an uninterrupted, 30amino acid domain (Fig. 1A). Proteins containing th ...
Detection of alien viruses and viroids in plants by siRNA
... ds secondary structures of single-stranded RNA virus genomes ds secondary structures of RNA transcripts produced by DNA viruses ...
... ds secondary structures of single-stranded RNA virus genomes ds secondary structures of RNA transcripts produced by DNA viruses ...
Chemistry of Life Chapter 3 ORGANIC CHEMISTRY Introduction
... leading to a helical coil or sheet like array (such as mica or fools gold). This structure results from hydrogen bonding of side groups on the amino acid chains. c. Tertiary structure: refers to the further folding due to interactions among R groups along the polypeptide chain. d. Quaternary structu ...
... leading to a helical coil or sheet like array (such as mica or fools gold). This structure results from hydrogen bonding of side groups on the amino acid chains. c. Tertiary structure: refers to the further folding due to interactions among R groups along the polypeptide chain. d. Quaternary structu ...
(1) Identify the secondary structure described in each of the
... a. Proteins possess quaternary structure only if they consist of two or more polypeptide chains (subunits). ...
... a. Proteins possess quaternary structure only if they consist of two or more polypeptide chains (subunits). ...
Gel electrophoresis
... organelle preparation is ready, a variety are available for separation of proteins. Ionexchange chromatography can be used to separate proteins with different charges (similar to the way amino acids are separated). Other chromatographic methods take advantage of differences in size, binding affinit ...
... organelle preparation is ready, a variety are available for separation of proteins. Ionexchange chromatography can be used to separate proteins with different charges (similar to the way amino acids are separated). Other chromatographic methods take advantage of differences in size, binding affinit ...
DNA to Protein Overview
... During protein translation amino acids are linked together to form a polypeptide chain which will later be folded into a protein. The ribosome is the workbench or factory for protein translation. It consists of a large and a small subunit (see graphic – Protein Translation). A ribosome can use any m ...
... During protein translation amino acids are linked together to form a polypeptide chain which will later be folded into a protein. The ribosome is the workbench or factory for protein translation. It consists of a large and a small subunit (see graphic – Protein Translation). A ribosome can use any m ...
A tale of two functions: enzymatic activity and
... The Zn finger of CT is a member of the Cys4-type family, with the divalent metal coordinated in a tetrahedral fashion by four cysteine sulfhydryl groups (Figure 1). Each of the cysteines (residues 27, 30, 46 and 49) coordinating the zinc atom were mutated to alanine individually followed by multiple ...
... The Zn finger of CT is a member of the Cys4-type family, with the divalent metal coordinated in a tetrahedral fashion by four cysteine sulfhydryl groups (Figure 1). Each of the cysteines (residues 27, 30, 46 and 49) coordinating the zinc atom were mutated to alanine individually followed by multiple ...
Macromolecules of the Cell
... Starch is found in plants. It occurs either as branched (amylopectin) or unbranched (amylase). Starch deposits are 10-30% amylase and 70-90% amylopectin. Starch is stored as starch grains within plastids-either chloroplasts (sites for sugar synthesis) or within amyloplasts (specialized for starch st ...
... Starch is found in plants. It occurs either as branched (amylopectin) or unbranched (amylase). Starch deposits are 10-30% amylase and 70-90% amylopectin. Starch is stored as starch grains within plastids-either chloroplasts (sites for sugar synthesis) or within amyloplasts (specialized for starch st ...
Unit 1 LE - SchneiderSBI4U
... respect to such properties as solubility, “electron-mobility” and relative stability with the presence of double- and triple-bonded carbons within their structures; ...
... respect to such properties as solubility, “electron-mobility” and relative stability with the presence of double- and triple-bonded carbons within their structures; ...
Techniques
... • The mRNA is then tagged with a fluorescent dye and incubated overnight with the microarray. • mRNA hybridize to spots on the microarray that contain complementary DNA sequences. • Microarray is washed and scanned by a laser that cause the mRNA hybridized to the microarray to fluoresce. • Tell you ...
... • The mRNA is then tagged with a fluorescent dye and incubated overnight with the microarray. • mRNA hybridize to spots on the microarray that contain complementary DNA sequences. • Microarray is washed and scanned by a laser that cause the mRNA hybridized to the microarray to fluoresce. • Tell you ...
Protein Structure and Function
... Folding, modification, and degradation of proteins The life of a protein can briefly be described as: synthesis, folding, modification, function, degradation. a A newly synthesized polypeptide chain must undergo folding and often chemical modification to generate the final protein a All molecules o ...
... Folding, modification, and degradation of proteins The life of a protein can briefly be described as: synthesis, folding, modification, function, degradation. a A newly synthesized polypeptide chain must undergo folding and often chemical modification to generate the final protein a All molecules o ...
Translation - Phillipsburg School District
... • Converts/transfers information from mRNA into amino acids • Amino acids are the monomers of proteins • String amino acids together and a protein is made • 3 RNAs needed – mRNA (messenger—from nucleus to ribosome) – rRNA (ribosomal—used in the ribosome) – tRNA (transfer—transfers the codons into am ...
... • Converts/transfers information from mRNA into amino acids • Amino acids are the monomers of proteins • String amino acids together and a protein is made • 3 RNAs needed – mRNA (messenger—from nucleus to ribosome) – rRNA (ribosomal—used in the ribosome) – tRNA (transfer—transfers the codons into am ...
proteins
... 1. A polypeptide is a polymer of amino acids connected in a specific sequence • Amino acids consist of four components attached to a central carbon, the alpha carbon. • These components include a hydrogen atom, a carboxyl group, an amino group, and a variable R group (or side chain). • Differences ...
... 1. A polypeptide is a polymer of amino acids connected in a specific sequence • Amino acids consist of four components attached to a central carbon, the alpha carbon. • These components include a hydrogen atom, a carboxyl group, an amino group, and a variable R group (or side chain). • Differences ...
RNA interference: the new somatic cell genetics?
... sequence of 27 nt from the U6 snRNA improves expression (Paul et al., 2002). Third, there is in vitro data to suggest that the mouse U6 promoter may be more active in human cells, given the strong affinity of the SNAP-c complex for the proximal sequence element (Chong et al., 2001). However, given t ...
... sequence of 27 nt from the U6 snRNA improves expression (Paul et al., 2002). Third, there is in vitro data to suggest that the mouse U6 promoter may be more active in human cells, given the strong affinity of the SNAP-c complex for the proximal sequence element (Chong et al., 2001). However, given t ...
Chapter 8
... • Translation is the process that converts a mRNA message into a protein. • The language of nucleic acids is A,C,T,G, & U’s, but the language of proteins is amino acids (remember these are the monomers of proteins) • The A,C,T,G, &U’s of a nucleic acid are in a very ...
... • Translation is the process that converts a mRNA message into a protein. • The language of nucleic acids is A,C,T,G, & U’s, but the language of proteins is amino acids (remember these are the monomers of proteins) • The A,C,T,G, &U’s of a nucleic acid are in a very ...
Gene expression
Gene expression is the process by which information from a gene is used in the synthesis of a functional gene product. These products are often proteins, but in non-protein coding genes such as transfer RNA (tRNA) or small nuclear RNA (snRNA) genes, the product is a functional RNA.The process of gene expression is used by all known life - eukaryotes (including multicellular organisms), prokaryotes (bacteria and archaea), and utilized by viruses - to generate the macromolecular machinery for life.Several steps in the gene expression process may be modulated, including the transcription, RNA splicing, translation, and post-translational modification of a protein. Gene regulation gives the cell control over structure and function, and is the basis for cellular differentiation, morphogenesis and the versatility and adaptability of any organism. Gene regulation may also serve as a substrate for evolutionary change, since control of the timing, location, and amount of gene expression can have a profound effect on the functions (actions) of the gene in a cell or in a multicellular organism.In genetics, gene expression is the most fundamental level at which the genotype gives rise to the phenotype, i.e. observable trait. The genetic code stored in DNA is ""interpreted"" by gene expression, and the properties of the expression give rise to the organism's phenotype. Such phenotypes are often expressed by the synthesis of proteins that control the organism's shape, or that act as enzymes catalysing specific metabolic pathways characterising the organism.