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Chapter 5 - Scranton Prep Biology
Chapter 5 - Scranton Prep Biology

... Phospholipiils Phospholipids consist of a glycerol linked to two fatty acids and a negatively charged phosphate group, to which other small moleculesmay be attached. The phosphate head of this molecule is hydrophilic and water soluble, whereas the two fatty acid chains are hydrophobic. The unique st ...
L2 Protein structure - e
L2 Protein structure - e

...  A domain is a basic structural unit within a protein molecule.  Part of protein that can fold into a stable structure independently.  Different domains can possess different functions.  Proteins can have one to many domains depending on protein size.  A polypeptide with 200 amino acids consist ...
Biology Dictionary
Biology Dictionary

... Adaptive radiation. The evolution of new species or sub-species to fill unoccupied ecological niches. Aerobe. A microorganism that grows in the presence of oxygen. See Anaerobe. Agarose gel electrophoresis. A matrix composed of a highly purified form of agar that is used to separate larger DNA and R ...
chapter 5 the structure and function of macromolecules
chapter 5 the structure and function of macromolecules

... almost everything that an organism does. ° Protein functions include structural support, storage, transport, cellular signaling, movement, and defense against foreign substances. ° Most important, protein enzymes function as catalysts in cells, regulating metabolism by selectively accelerating chemi ...
Uniikki kuitu
Uniikki kuitu

... Proteins or protein domains belonging to a particular family generally share functional attributes and are derived from a common ancestor.It is apparent, when studying protein sequence families, that some regions have been better conserved than others during evolution. ...
Fill in blank notes - Cathkin High School
Fill in blank notes - Cathkin High School

... removed along with the intron(s) next to them. [All the ___________ are always removed from the primary RNA transcript]. This is called _______________ RNA splicing. This means that the same primary mRNA transcript has the potential to produce several different mRNA molecules. Each mRNA molecule wil ...
Strong association between mRNA folding strength and protein
Strong association between mRNA folding strength and protein

... The mF strength of a transcript (or a part of it) was defined as the mean PARS score over the sequence; higher values of this measure correspond to stronger folding. We found the correlation between PA (the mean of four data sets, Methods) and mF strength to be 0.68 (P ¼ 10200; Fig 1A); thus, excep ...
MOL WS 2016 Handout T3 Metabolism RNA world
MOL WS 2016 Handout T3 Metabolism RNA world

... It is hypothesized that pre-mRNA splicing may have evolved from group II introns, due to the similar catalytic mechanism as well as the structural similarity of the Domain V substructure to the U6/U2 extended snRNA ...
Usha`s presentation - The University of Texas at Dallas
Usha`s presentation - The University of Texas at Dallas

... Holm L., Sander C(1993 a) Protein Structure Comparison by Alignment of Distance Matrices. Journal of Molecular Biol. 233(1): 123-138 Holm L., Park J(2000) DaliLite workbench for protein structure comparison. Bioinformatics 16, 566-567 Holm L., Sander C(1996) Mapping the protein ...
Powerpoint on Proteins
Powerpoint on Proteins

... describing protein signaling. • Proteins have to be transported either out of the cell, or to the different compartments - the organelles - within the cell. ...
Working concentrations and stock solutions
Working concentrations and stock solutions

... 1. Prepare and autoclave/sterilize stock media. Be sure that the flask contains a stir-bar. 2. The solution must cool before adding antibiotics as the heat may inactive them. Let the flask equilibrate in the water bath set at 55-60o C for a minimum of 30 min. At this point, agar solutions should be ...
Basics of BLAST - GEP Community Server
Basics of BLAST - GEP Community Server

... - Protein function exploration - Novel gene  make parameters more sensitive ...
$doc.title

... Empty Vector ...
Translation
Translation

... – Charged tRNA enters. Hbonding established between codon (mRNA) and anticodon of (tRNA). Sites include regions of large and small subunits of ribosome. ...
Mass Spectrometry of Peptides
Mass Spectrometry of Peptides

... MS/MS plays important role in protein identification (fast and sensitive) Derivation of peptide sequence an important task in proteomics Derivation without help from a protein database (“de novo sequencing”), especially important in identification of unknown protein ...
Manipulating DNA - tools and techniques 2012
Manipulating DNA - tools and techniques 2012

...  6 x 109 base pairs of DNA  20,00 – 25,000 genes  Sequences of non-coding DNA ...
Lecture 27
Lecture 27

Proteins
Proteins

... Steps of Chaperonin Action: An unfolded polypeptide enters the cylinder from one end. ...
Slide 1
Slide 1

...  Post-translational modifications (PTMs)  Covalent processing events that change the properties of a protein  proteolytic cleavage  addition of a modifying group to one or more amino acids  Determine its activity state, localization, turnover, interactions with other proteins  Mass spectrometr ...
Chapter 10 Protein Synthesis
Chapter 10 Protein Synthesis

... leaving the nucleus as mRNA  Messenger RNA (mRNA) – encodes amino acid sequences and – conveys genetic messages from DNA to the translation machinery of the cell, which in – prokaryotes, occurs in the same place that mRNA is made, but in – eukaryotes, mRNA must exit the nucleus via nuclear pores to ...
ppt - Chair of Computational Biology
ppt - Chair of Computational Biology

... They are transcribed by RNA polymerase II or RNA polymerase III and are involved in a variety of important processes such as - RNA splicing, - regulation of transcription factors or RNA polymerase II, and - maintaining the telomeres. snRNAs are always associated with specific proteins. The snRNA:pro ...
QTL analysis of yield traits in an advanced backcross
QTL analysis of yield traits in an advanced backcross

... 413 bp ORF, starting with an initiating codon at 238 bp and ending with a stop codon at 1 650 bp (accession number FJ768729). The predicted protein product of AhKASⅠ comprises 470 amino acids with the calculated molecular mass of 49.958 9 kD and a pI of 8.46. Prediction of subcellular location sugge ...
DNA
DNA

... – Transcription is the process of creating an RNA strand from a template of DNA nucleotides ...
Gene - CS273a
Gene - CS273a

... Insulators are DNA sequences that when placed between target gene and enhancer prevent enhancer from acting on the gene. •The handful known insulators contain binding sites for a specific DNA binding protein (CTCF) that is involved in DNA 3D conformation. •However, CTCF fulfills additional roles bes ...
w0506_tutorial8
w0506_tutorial8

... Can BLAST help us to predict its SS? 2. Use any secondary structure prediction method to predict the secondary structure of 1O8V and compare it to the solved structure. NOTICE! The secondary structure definition in PDB is given in a 7 letter code instead of 3 letter code (H, E, C). For comparison pu ...
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Gene expression



Gene expression is the process by which information from a gene is used in the synthesis of a functional gene product. These products are often proteins, but in non-protein coding genes such as transfer RNA (tRNA) or small nuclear RNA (snRNA) genes, the product is a functional RNA.The process of gene expression is used by all known life - eukaryotes (including multicellular organisms), prokaryotes (bacteria and archaea), and utilized by viruses - to generate the macromolecular machinery for life.Several steps in the gene expression process may be modulated, including the transcription, RNA splicing, translation, and post-translational modification of a protein. Gene regulation gives the cell control over structure and function, and is the basis for cellular differentiation, morphogenesis and the versatility and adaptability of any organism. Gene regulation may also serve as a substrate for evolutionary change, since control of the timing, location, and amount of gene expression can have a profound effect on the functions (actions) of the gene in a cell or in a multicellular organism.In genetics, gene expression is the most fundamental level at which the genotype gives rise to the phenotype, i.e. observable trait. The genetic code stored in DNA is ""interpreted"" by gene expression, and the properties of the expression give rise to the organism's phenotype. Such phenotypes are often expressed by the synthesis of proteins that control the organism's shape, or that act as enzymes catalysing specific metabolic pathways characterising the organism.
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