oL-Amylase of Clostridium thermosulfurogenes EMi:
... enzymatically functioning regions. The tentative Ca2+-binding site (consensus region I) of this Ca2+independent enzyme showed only limited homology. The deduced amino acid sequence of a second obviously truncated open reading frame showed significant homology to the malG gene product of E. coli. Com ...
... enzymatically functioning regions. The tentative Ca2+-binding site (consensus region I) of this Ca2+independent enzyme showed only limited homology. The deduced amino acid sequence of a second obviously truncated open reading frame showed significant homology to the malG gene product of E. coli. Com ...
local alignments - LSU School of Medicine
... 2) Sequences above a certain threshold (< specified E-value) are included. Assumed to be related proteins. This group of sequences is used to define a “profile” that contains the sequence essence of the “family”. 3) Now with the important sequence positions highlighted, can look for more distantly r ...
... 2) Sequences above a certain threshold (< specified E-value) are included. Assumed to be related proteins. This group of sequences is used to define a “profile” that contains the sequence essence of the “family”. 3) Now with the important sequence positions highlighted, can look for more distantly r ...
Follow Monty Python's Footsteps: Towards the Holy Grail of Protein Structure Prediction
... beta strands means that adjacent residues have their side chains pointing in oppposite directions. Beta strands that are half buried in the protein core will tend to have hydrophobic residues at positions i, i+2, i+4, i+8 etc, and polar residues at positions i+1, i+3, i+5, etc. For example, this be ...
... beta strands means that adjacent residues have their side chains pointing in oppposite directions. Beta strands that are half buried in the protein core will tend to have hydrophobic residues at positions i, i+2, i+4, i+8 etc, and polar residues at positions i+1, i+3, i+5, etc. For example, this be ...
De Novo Mutation Found in the Porphobilinogen Deaminase Gene
... symptomatology, we identified de novo mutation 966insA within the PBGD gene leading to a stop codon after 36 completely different amino acids compared to the wt-sequence. To establish the effects of this mutation on the protein structure, we expressed mutant constructs with described mutation in E. ...
... symptomatology, we identified de novo mutation 966insA within the PBGD gene leading to a stop codon after 36 completely different amino acids compared to the wt-sequence. To establish the effects of this mutation on the protein structure, we expressed mutant constructs with described mutation in E. ...
Neutrophil-specific granule deficiency: homozygous recessive
... Patient, materials, and methods SGD proband and genomic DNA isolation The proband is a 27-year old female who has suffered from recurrent pyogenic infections, such as otitis media, skin abscesses, and pneumonia, since infancy. As previously reported,3,8 the neutrophils of this individual exhibit uni ...
... Patient, materials, and methods SGD proband and genomic DNA isolation The proband is a 27-year old female who has suffered from recurrent pyogenic infections, such as otitis media, skin abscesses, and pneumonia, since infancy. As previously reported,3,8 the neutrophils of this individual exhibit uni ...
protein metabolism - cmb
... plot of isotope remaining in a protein versus time is linear. Thus, we can determine the metabolic half-life of a particular protein. In a rat the average protein has a half-life of 1 or 2 days. ...
... plot of isotope remaining in a protein versus time is linear. Thus, we can determine the metabolic half-life of a particular protein. In a rat the average protein has a half-life of 1 or 2 days. ...
... balanced amino acid profile in accordance with the specific human pattern. All living organisms, including humans, have a characteristic amino acid pattern – known as the “Master Amino Acid Pattern.” MAP® provides the eight essential amino acids in a unique ratio for human nutrition. This is how MAP ...
Nuclear and mitochondrial forms of human uracil
... (13,14). They are, however, unrelated to the group of homologous uracil-DNA glycosylases, as well as to each other. The nuclear form of uracil-DNA glycosylase is far more abundant than the mitochondrial form (7,15,16). Transcription of the UNG gene is up-regulated in the late Gl-phase, accounting fo ...
... (13,14). They are, however, unrelated to the group of homologous uracil-DNA glycosylases, as well as to each other. The nuclear form of uracil-DNA glycosylase is far more abundant than the mitochondrial form (7,15,16). Transcription of the UNG gene is up-regulated in the late Gl-phase, accounting fo ...
Genetic Code
... this tRNA, and which amino acid also bound in the active site of that enzyme, then you would know which amino acid will be found on this tRNA. And then you'd know what amino acid would go into the polypeptide when the mRNA had the codon UGG, which is complementary to this tRNA's anticodon. To make t ...
... this tRNA, and which amino acid also bound in the active site of that enzyme, then you would know which amino acid will be found on this tRNA. And then you'd know what amino acid would go into the polypeptide when the mRNA had the codon UGG, which is complementary to this tRNA's anticodon. To make t ...
Protein Structure
... • A beta strand is a region of a polypeptide that is composed of the Vshaped N-C-C backbone of the amino acids alternating up and down. The R groups also alternate up and down. • Two or more beta strands next to each other is a beta pleated sheet. • Beta sheets are held together by hydrogen bonds be ...
... • A beta strand is a region of a polypeptide that is composed of the Vshaped N-C-C backbone of the amino acids alternating up and down. The R groups also alternate up and down. • Two or more beta strands next to each other is a beta pleated sheet. • Beta sheets are held together by hydrogen bonds be ...
Powerpoint Slides
... CONCENTRATION of nucleic acid. •Plots of this are called Cot curves, which are much like ...
... CONCENTRATION of nucleic acid. •Plots of this are called Cot curves, which are much like ...
Investigation of the starch-binding properties of wheat friabilin
... 15kDa (under reducing conditions). The molecular weight calculated from the DNA sequences is 14kDa for both PA and GSPP. The difference between the calculated and apparent molecular weights of PA and GSPP might be caused by the large number of basic amino acid residues present in these polypeptides. ...
... 15kDa (under reducing conditions). The molecular weight calculated from the DNA sequences is 14kDa for both PA and GSPP. The difference between the calculated and apparent molecular weights of PA and GSPP might be caused by the large number of basic amino acid residues present in these polypeptides. ...
Proteomics_12-6
... - scores from decoy comparison are included, which can provide some idea of the distribution of ‘incorrect’ scores -EM or Bayesian approaches can then estimate the proportion of correct vs. incorrect PSM … based on each PSM score, a posterior probability is calculated ...
... - scores from decoy comparison are included, which can provide some idea of the distribution of ‘incorrect’ scores -EM or Bayesian approaches can then estimate the proportion of correct vs. incorrect PSM … based on each PSM score, a posterior probability is calculated ...
A Drosophila Third Chromosome Minute Locus Encodes
... M i n u t e s are non-additive in their phenotypic effect, i. e., the phenotype of a M I / + ; M 2 / + fly is not more extreme than the phenotypeof any ofthe single mutants. He concluded that the genes code for proteins with similar function (s) . The non-additive property of this type of mutations ...
... M i n u t e s are non-additive in their phenotypic effect, i. e., the phenotype of a M I / + ; M 2 / + fly is not more extreme than the phenotypeof any ofthe single mutants. He concluded that the genes code for proteins with similar function (s) . The non-additive property of this type of mutations ...
lecture10_15_new
... Modeling of a sequence based on known structures Consist of four major steps : 1. Finding a known structure(s) related to the sequence to be modeled (template), using sequence comparison methods such as PSI-BLAST 2. Aligning sequence with the templates 3. Building a model 4. Assessing the model ...
... Modeling of a sequence based on known structures Consist of four major steps : 1. Finding a known structure(s) related to the sequence to be modeled (template), using sequence comparison methods such as PSI-BLAST 2. Aligning sequence with the templates 3. Building a model 4. Assessing the model ...
Genomics Core, Dr. Yuannan Xia
... Arabidopsis mitochondrial genome (S. Mackenzie) Small RNA sequencing Several UNL faculty have expressed strong interest. (Y.Bin, H. Cerutti, J. Mower, J. Alfano) ...
... Arabidopsis mitochondrial genome (S. Mackenzie) Small RNA sequencing Several UNL faculty have expressed strong interest. (Y.Bin, H. Cerutti, J. Mower, J. Alfano) ...
Problem Sets / Exams - Department of Chemistry ::: CALTECH
... a. (5 points) If a 50 amino acid polypeptide were to sample all of its possible conformations in order to fold, how long would this process take? Assume that each amino acid residue can have three different conformations and it takes one picosecond (10-12) to convert between structures. b. (5 points ...
... a. (5 points) If a 50 amino acid polypeptide were to sample all of its possible conformations in order to fold, how long would this process take? Assume that each amino acid residue can have three different conformations and it takes one picosecond (10-12) to convert between structures. b. (5 points ...
Complete nucleotide sequences of two soybean
... Department of Plant Pathology, Iowa State University, Ames, Iowa 50011, U.S.A. ...
... Department of Plant Pathology, Iowa State University, Ames, Iowa 50011, U.S.A. ...
The HSP90 family of genes in the human genome
... Introduction HSP90 proteins, named according to the 90-kDa average molecular mass of their members, are highly conserved molecular chaperones that account for 1 – 2% of all cellular proteins in most cells under non-stress conditions [1]. HSP90 proteins have key roles in signal transduction, protein ...
... Introduction HSP90 proteins, named according to the 90-kDa average molecular mass of their members, are highly conserved molecular chaperones that account for 1 – 2% of all cellular proteins in most cells under non-stress conditions [1]. HSP90 proteins have key roles in signal transduction, protein ...
Experiment 2 Determination of DNA Concentration and - RIT
... It is clear from the above table that single-stranded DNA absorbs more UV than double stranded DNA. This is due to interactions between the stacked bases in double-stranded DNA. The difference can be also demonstrated directly by comparing the OD’s of double-stranded DNA and DNA that has been denatu ...
... It is clear from the above table that single-stranded DNA absorbs more UV than double stranded DNA. This is due to interactions between the stacked bases in double-stranded DNA. The difference can be also demonstrated directly by comparing the OD’s of double-stranded DNA and DNA that has been denatu ...
Expression and purification of proteins using Strep
... promoter/operator region from the tetA resistance gene and are the state-of-the-art solution for such an inducible expression system [4, 5]. The strength of the tetA promoter is comparable with that of the lac-UV5 promoter. The protein expression can be induced by adding anhydrotetracycline at a con ...
... promoter/operator region from the tetA resistance gene and are the state-of-the-art solution for such an inducible expression system [4, 5]. The strength of the tetA promoter is comparable with that of the lac-UV5 promoter. The protein expression can be induced by adding anhydrotetracycline at a con ...
1 Lecture 20: Analysis of Enzyme Inhibition
... In most cases chromatography is performed in long glass tubes filled with a matrix or resin (particle size similar to a fine sand) that is completely immersed in a buffered salt solution. The mixture of proteins is added to the top of this column and buffer is allowed to flow through the column. As ...
... In most cases chromatography is performed in long glass tubes filled with a matrix or resin (particle size similar to a fine sand) that is completely immersed in a buffered salt solution. The mixture of proteins is added to the top of this column and buffer is allowed to flow through the column. As ...
N-terminal amino acid sequences of chloroform/methanol
... valúes of about 2) of castor bean (Ricinus communis) [10,11] and oilseed rape (Brassica napus) [12] (see fig.l). Both proteins are composed of two subunits which are associated by disulphide bonds [10,32]. The small subunits (Mr valúes about 4000) are homologous with the N-terminal regions of the « ...
... valúes of about 2) of castor bean (Ricinus communis) [10,11] and oilseed rape (Brassica napus) [12] (see fig.l). Both proteins are composed of two subunits which are associated by disulphide bonds [10,32]. The small subunits (Mr valúes about 4000) are homologous with the N-terminal regions of the « ...
Slides
... • The amino acid sequence determines the structure which determines the function. • Proteins make up over 50% of the cell by dry weight. • Proteins give the cell its shape, they form receptors, enzymes, hormones and growth factors, toxins, transporters and antibodies. ...
... • The amino acid sequence determines the structure which determines the function. • Proteins make up over 50% of the cell by dry weight. • Proteins give the cell its shape, they form receptors, enzymes, hormones and growth factors, toxins, transporters and antibodies. ...
Gene expression
Gene expression is the process by which information from a gene is used in the synthesis of a functional gene product. These products are often proteins, but in non-protein coding genes such as transfer RNA (tRNA) or small nuclear RNA (snRNA) genes, the product is a functional RNA.The process of gene expression is used by all known life - eukaryotes (including multicellular organisms), prokaryotes (bacteria and archaea), and utilized by viruses - to generate the macromolecular machinery for life.Several steps in the gene expression process may be modulated, including the transcription, RNA splicing, translation, and post-translational modification of a protein. Gene regulation gives the cell control over structure and function, and is the basis for cellular differentiation, morphogenesis and the versatility and adaptability of any organism. Gene regulation may also serve as a substrate for evolutionary change, since control of the timing, location, and amount of gene expression can have a profound effect on the functions (actions) of the gene in a cell or in a multicellular organism.In genetics, gene expression is the most fundamental level at which the genotype gives rise to the phenotype, i.e. observable trait. The genetic code stored in DNA is ""interpreted"" by gene expression, and the properties of the expression give rise to the organism's phenotype. Such phenotypes are often expressed by the synthesis of proteins that control the organism's shape, or that act as enzymes catalysing specific metabolic pathways characterising the organism.