Topic 6 – Making Recombinant DNA Recombinant DNA – fragment
... § Taq DNA polymerase is found in the bacterium Thermos aquaticus, which lives at extremely high temperatures ...
... § Taq DNA polymerase is found in the bacterium Thermos aquaticus, which lives at extremely high temperatures ...
NAME: CLASS:______ DNA - The Double Helix Recall that the
... activities of the cell including cell reproduction, and heredity. How does it do this? The nucleus controls these activities by the chromosomes. Chromosomes are microscopic, threadlike strands composed of the chemical DNA (short for deoxyribonucleic acid. In simple terms, DNA controls the production ...
... activities of the cell including cell reproduction, and heredity. How does it do this? The nucleus controls these activities by the chromosomes. Chromosomes are microscopic, threadlike strands composed of the chemical DNA (short for deoxyribonucleic acid. In simple terms, DNA controls the production ...
highly repetitive DNA
... the length into which it’s packaged • Smallest human chromosome (21) has 4x107 bp of DNA, 10 times size of E. coli genome • Equivalent to 14mm of extended DNA • In most condensed state the chromosome is about 2mm long • Packing ratio = 14000/2 = 7000 • So, there must be an efficient packaging mechan ...
... the length into which it’s packaged • Smallest human chromosome (21) has 4x107 bp of DNA, 10 times size of E. coli genome • Equivalent to 14mm of extended DNA • In most condensed state the chromosome is about 2mm long • Packing ratio = 14000/2 = 7000 • So, there must be an efficient packaging mechan ...
Mutations - Choteau Schools
... problems in the cells or the organism. At times, the problem is so severe that the organism does not survive. ...
... problems in the cells or the organism. At times, the problem is so severe that the organism does not survive. ...
Regulation of Gene Expression – Part III
... ___________, may “jump” from location to another, disrupting one or more genes…leading to an abnormal product Interrupted DNA sequence ...
... ___________, may “jump” from location to another, disrupting one or more genes…leading to an abnormal product Interrupted DNA sequence ...
Protein Synthesis Bead Activity
... mRNA strand are called a ______________. A codon codes for one ____________________. We need these monomers because we are making _____________________. Now that we have the place to build the protein and the copied instructions on how to make the protein, the parts (amino acids) need to be brought ...
... mRNA strand are called a ______________. A codon codes for one ____________________. We need these monomers because we are making _____________________. Now that we have the place to build the protein and the copied instructions on how to make the protein, the parts (amino acids) need to be brought ...
BI 200 – Final Exam
... 43. A piece of double stranded DNA has 14% Adenine bases. Which of the following would not be true? a. there would be 36% cytosine in the DNA b. there would be 14% uracil in the DNA c. there would be 36% guanine in the DNA d. none of the above, all are true in the DNA 44. The inability to ferment l ...
... 43. A piece of double stranded DNA has 14% Adenine bases. Which of the following would not be true? a. there would be 36% cytosine in the DNA b. there would be 14% uracil in the DNA c. there would be 36% guanine in the DNA d. none of the above, all are true in the DNA 44. The inability to ferment l ...
Protocol for T4 Polynucleotide Kinase, Cloned
... T4 Polynucleotide Kinase (T4 PNK) catalyzes the transfer of the γ-phosphate of ATP to the 5′ terminus of single- and double-stranded DNA or RNA molecules that have a 5′ hydroxyl. The enzyme also removes the 3′ phosphate from 3′-phosphoryl polynucleotides, deoxyribonucleoside 3′-monophosphates, and d ...
... T4 Polynucleotide Kinase (T4 PNK) catalyzes the transfer of the γ-phosphate of ATP to the 5′ terminus of single- and double-stranded DNA or RNA molecules that have a 5′ hydroxyl. The enzyme also removes the 3′ phosphate from 3′-phosphoryl polynucleotides, deoxyribonucleoside 3′-monophosphates, and d ...
word - marric
... 5b. Students know how to apply base-pairing rules to explain precise copying of DNA during semiconservative replication and transcription of information from DNA into mRNA. Enzymes initiate DNA replication by unwinding and unzipping, the double helix to separate the two parental strands. Each strand ...
... 5b. Students know how to apply base-pairing rules to explain precise copying of DNA during semiconservative replication and transcription of information from DNA into mRNA. Enzymes initiate DNA replication by unwinding and unzipping, the double helix to separate the two parental strands. Each strand ...
4. Protein Synthesis and Biotechnology
... Enzymes initiate DNA replication by unzipping, or unwinding, the double helix to separate the two parental strands. Each strand acts as a template to form a complementary daughter strand of DNA. The new daughter strands are formed when complementary new nucleotides are added to the bases of the nucl ...
... Enzymes initiate DNA replication by unzipping, or unwinding, the double helix to separate the two parental strands. Each strand acts as a template to form a complementary daughter strand of DNA. The new daughter strands are formed when complementary new nucleotides are added to the bases of the nucl ...
投影片 1
... RNA strand does not remain H bond to the DNA template and quickly releases from the DNA as it synthesize for efficient RNA production. RNA polymerase can catalyze the formation of phosphodiester bond( RNA’s sugar-phosphate backbone). But it dose not check the correctness of previous base-pair, which ...
... RNA strand does not remain H bond to the DNA template and quickly releases from the DNA as it synthesize for efficient RNA production. RNA polymerase can catalyze the formation of phosphodiester bond( RNA’s sugar-phosphate backbone). But it dose not check the correctness of previous base-pair, which ...
Biocatalysis - Chatham University
... ESSENTIALS STEPS IN DNA CLONING 1. Cutting target DNA at precise locations. Sequence-specific endonucleases (restriction endonucleases) provide the necessary molecular scissors 2. Selecting a small carrier molecule of DNA capable of selfreplication. These DNAs are called cloning vectors (typically ...
... ESSENTIALS STEPS IN DNA CLONING 1. Cutting target DNA at precise locations. Sequence-specific endonucleases (restriction endonucleases) provide the necessary molecular scissors 2. Selecting a small carrier molecule of DNA capable of selfreplication. These DNAs are called cloning vectors (typically ...
Epigenetics.ppt
... Epigenetics and DNA Methylation: • Differential gene expression allows different cell types to adopt different fates. • Mistakes in gene regulation are associated with a variety of diseases, especially cancer. ...
... Epigenetics and DNA Methylation: • Differential gene expression allows different cell types to adopt different fates. • Mistakes in gene regulation are associated with a variety of diseases, especially cancer. ...
Sample Exam 3 Questions
... nascent protein chain from the carboxyl to the amino terminus. Ribosomes read mRNA from the 3' to the 5' end and synthesize the nascent protein chain from the amino to the carboxyl terminus. Ribosomes read mRNA from the 5' to the 3' end and synthesize the nascent protein chain from the amino to the ...
... nascent protein chain from the carboxyl to the amino terminus. Ribosomes read mRNA from the 3' to the 5' end and synthesize the nascent protein chain from the amino to the carboxyl terminus. Ribosomes read mRNA from the 5' to the 3' end and synthesize the nascent protein chain from the amino to the ...
DNA STRUCTURE
... DIVALENT CATIONS ARE GOOD SHIELDING AGENTS MONOVALENT CATIONS INTERACT NON-SPECIFICALLY ...
... DIVALENT CATIONS ARE GOOD SHIELDING AGENTS MONOVALENT CATIONS INTERACT NON-SPECIFICALLY ...
Microbial Genetics and Taxonomy
... • Genes – specific sequences of nucleotides that code for polypeptides • Genomes - sum of all genetic material in a cell or virus • Prokaryotic and eukaryotic cells – use DNA as genetic materials • Viruses – use either DNA or RNA ...
... • Genes – specific sequences of nucleotides that code for polypeptides • Genomes - sum of all genetic material in a cell or virus • Prokaryotic and eukaryotic cells – use DNA as genetic materials • Viruses – use either DNA or RNA ...
Let`s make some animal proteins using DNA!!
... What is “transcription?” • A part of the DNA double helix within the nucleus is unzipped, cut by enzymes, and then copied onto a new single strand, called mRNA. This process is called “transcription.” • Once the DNA is transcribed, the single strand moves from the nucleus to a ribosome in the cytop ...
... What is “transcription?” • A part of the DNA double helix within the nucleus is unzipped, cut by enzymes, and then copied onto a new single strand, called mRNA. This process is called “transcription.” • Once the DNA is transcribed, the single strand moves from the nucleus to a ribosome in the cytop ...
Lecture 6: DNA and molecular genetics
... (antisense). • Bases pair up by weak bonds allowing for easy copying and replication of the DNA strands: the strands unzip and build two more by addiing bases to each strand. The template also acts as quality control to check for correct pairing and error correction if necessary • DNA is transcribed ...
... (antisense). • Bases pair up by weak bonds allowing for easy copying and replication of the DNA strands: the strands unzip and build two more by addiing bases to each strand. The template also acts as quality control to check for correct pairing and error correction if necessary • DNA is transcribed ...
How are animal proteins made from DNA?
... What is “transcription?” • A part of the DNA double helix within the nucleus is unzipped, cut by enzymes, and then copied onto a new single strand, called mRNA. This process is called “transcription.” • Once the DNA is transcribed, the single strand moves from the nucleus to a ribosome in the cytop ...
... What is “transcription?” • A part of the DNA double helix within the nucleus is unzipped, cut by enzymes, and then copied onto a new single strand, called mRNA. This process is called “transcription.” • Once the DNA is transcribed, the single strand moves from the nucleus to a ribosome in the cytop ...
Replisome
The replisome is a complex molecular machine that carries out replication of DNA. The replisome first unwinds double stranded DNA into two single strands. For each of the resulting single strands, a new complementary sequence of DNA is synthesized. The net result is formation of two new double stranded DNA sequences that are exact copies of the original double stranded DNA sequence.In terms of structure, the replisome is composed of two replicative polymerase complexes, one of which synthesizes the leading strand, while the other synthesizes the lagging strand. The replisome is composed of a number of proteins including helicase, RFC, PCNA, gyrase/topoisomerase, SSB/RPA, primase, DNA polymerase I, RNAse H, and ligase.