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Molecular Genetics of Viruses
Molecular Genetics of Viruses

... place fo some the viral DNA. – When this aberrant virus infects another cell, the bacterial DNA that it delivers can recombine with the resident DNA. ...
MUTATIONS
MUTATIONS

Homework Assignment #7
Homework Assignment #7

... the top strand are labeled. Use the lines to illustrate a eukaryotic gene that has two introns. Include the following in your drawing: promoter, transcription start site, all exons, both introns, the 5’ and 3’ splice site of the introns, a reasonable location for the ATG start codon and a TAA stop c ...
Chapter 11 ~ DNA and the Language of Life
Chapter 11 ~ DNA and the Language of Life

... • While DNA has the information for all the cell’s activities, it is not directly involved in the day to day operations of the cell. – Proteins are responsible for implementing the instructions contained in DNA ...
DNA and Protein Synthesis Review Questions w/answers
DNA and Protein Synthesis Review Questions w/answers

... e. Once there, the mRNA will be read in groups of 3 bases called __________. ...
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... Seals (repairs) single-strand nicks remaining D B. Primase between adjacent Okazaki fragments in discontinuous replication on lagging strand Stabilizes DNA single-strands/prevents them G C. Helicase from re-forming double-stranded DNA Removes ("relaxes") the positive supercoiling A D. DNA Ligase int ...
9.1 Manipulating DNA
9.1 Manipulating DNA

... 9.1 Manipulating DNA Restriction enzymes cut DNA. • Restriction enzymes act as “molecular scissors.” – come from various types of bacteria – allow scientists to more easily study and manipulate genes – cut DNA at a specific nucleotide sequence called a restriction site ...
9.1 Manipulating DNA KEY CONCEPT Biotechnology relies on cutting DNA at specific places.
9.1 Manipulating DNA KEY CONCEPT Biotechnology relies on cutting DNA at specific places.

... 9.1 Manipulating DNA Restriction enzymes cut DNA. • Restriction enzymes act as “molecular scissors.” – come from various types of bacteria – allow scientists to more easily study and manipulate genes – cut DNA at a specific nucleotide sequence called a restriction site ...
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... ____ 18. A mutant form of E. coli has been discovered in which replication is unsuccessful because the RNA primer is not released from the template strand. Which enzyme do you think is nonfunctional? a. DNA polymerase III d. Helicase b. Primase e. DNA polymerase I c. Ligase ____ 19. What was the rol ...
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... 7. In an experiment, a group that receives no treatment is called a(n) __control____ group. 8. In an experiment, the factor that I change/manipulate/vary is called the __independent________________ variable. 9. In an experiment, the factor that is measured is called the _dependent______ variable. 10 ...
Sample Examination Questions for Exam 3 Material
Sample Examination Questions for Exam 3 Material

... nascent protein chain from the carboxyl to the amino terminus. Ribosomes read mRNA from the 3' to the 5' end and synthesize the nascent protein chain from the amino to the carboxyl terminus. Ribosomes read mRNA from the 5' to the 3' end and synthesize the nascent protein chain from the amino to the ...
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... From scale invariance to deterministic chaos in DNA sequences : towards a deterministic description of gene organization in the human genome S. NICOLAY, E.B. BRODIE OF BRODIE, M. TOUCHON, Y. D’AUBENTON-CARAFA, THERMES & A. ARNEODO, Physica A (2004), to appear ...
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The Structure of a DNA Molecule
The Structure of a DNA Molecule

... Steps in the process of Replication Enzyme Helicase unwinds the DNA helix (1A) A Y-shaped Replication Fork results (1B) Single stranded DNA binding proteins prevent the strands from recombining (1C) Topoisomerase removes any twists or knots that form (1D) RNA Primase initiates DNA replication at spe ...
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... 1. __________________: The DNA molecule ‘Unzips’ as the _________________ between the base pairs are ______________. The enzyme __________________ causes this unzipping to occur. 2. _____________________ _______________________: Complementary __________ _____________________ to bond with the complem ...
Abstract-Template-2016
Abstract-Template-2016

... During analysis of the promoter region of the human MEST gene, we noted apparent nonMendelian behaviour of three closely linked single nucleotide polymorphisms (SNPs) 1. MEST plays a role in mammalian development and maternal behaviour. It is genomically imprinted, with the maternally inherited alle ...
Introduction to Molecular Genetics
Introduction to Molecular Genetics

... contained a highly viscous substance having the properties of DNA  Incubatyion with trypsin or chymotrypsin, enzymes that catalzye protein hydrolysis or with ribonuclease( RNase), an enzyme that catalyzes RNA hydrolysis did not affect the transforming principle Incubation with DNase, an enzyme that ...
Abstract-Template-2017 - Queenstown Research Week
Abstract-Template-2017 - Queenstown Research Week

Genética Molecular em Medicina Transfusional
Genética Molecular em Medicina Transfusional

... • The DNA sequencing rxn is similar to the PCR rxn. • The rxn mix includes the template DNA, Taq polymerase, dNTPs, ddNTPs, and a primer: a small piece of single-stranded DNA 20-30 nt long that hybridizes to one strand of the template DNA. • The rxn is intitiated by heating until the two strands of ...
GEL ELECTROPHORESIS
GEL ELECTROPHORESIS

... 1.) DNA is extracted by opening the cells and separating the DNA from other cell parts. 2.) DNA molecules are cut precisely into smaller fragments using restriction enzymes. Each restriction enzyme cuts DNA at a specific sequence of nucleotide bases. The R.E. will only cut a DNA sequence of if match ...
Chapter 25: Molecular Basis of Inheritance
Chapter 25: Molecular Basis of Inheritance

... complementary positions are joined by DNA polymerase. The process is semiconservative because each new double helix is composed of an old strand of nucleotides from the parent molecule and one newly-formed strand. Some cancer treatments are aimed at stopping DNA replication in rapidlydividing cancer ...
Tutorial What is DNA? http://learn.genetics.utah.edu/content
Tutorial What is DNA? http://learn.genetics.utah.edu/content

... 1. Click ‘Next’ to enter the inner ear, read and follow along.  2. Where do the instructions for a cell come from? _____________________________________  3. What does DNA stand for? _________________________________  4. What type of shape is a DNA molecule? __________________________________  5. Wha ...
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Replisome



The replisome is a complex molecular machine that carries out replication of DNA. The replisome first unwinds double stranded DNA into two single strands. For each of the resulting single strands, a new complementary sequence of DNA is synthesized. The net result is formation of two new double stranded DNA sequences that are exact copies of the original double stranded DNA sequence.In terms of structure, the replisome is composed of two replicative polymerase complexes, one of which synthesizes the leading strand, while the other synthesizes the lagging strand. The replisome is composed of a number of proteins including helicase, RFC, PCNA, gyrase/topoisomerase, SSB/RPA, primase, DNA polymerase I, RNAse H, and ligase.
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