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Extranuclear Inheritance
Extranuclear Inheritance

... Ÿ Size: 16kb for Humans & other mammals 18kb for Xenopus & Drosophila 80kb for Yeast 500kb for Corn Ÿ Shape: Circular, with supercoiling (like Prokaryote) Ÿ Replication: w Normal DNA Replication Process w Uses its own DNA polymerase w Occurs at any time in the cell cycle w Single origin of replicati ...
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4.4 Genetic engineering and biotechnology – summary of mark

... Outline a basic technique used for gene transfer involving plasmids, a host cell (bacterium, yeast or other cell), restriction enzymes (endonucleases) and DNA ligase. Mark Scheme A. B. C. D. E. F. G. H. I. J. K. L. ...
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Recap of 8.1 and 8.2
Recap of 8.1 and 8.2

... So how is the structure of DNA linked to its function? 1. DNA is very stable: It passes from generation to generation without changing. 2. The two strands are linked only by hydrogen bonds: During DNA replication and protein synthesis, the strands can separate easily. 3. It’s a huge molecule: It ca ...
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Spring 2005 - Antelope Valley College
Spring 2005 - Antelope Valley College

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7.014 Problem Set 3
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... 1. DNA replication (a) Why is DNA replication an essential process? In order for an organism to grow, its’ cells need to divide. For each round of cell division, DNA has to be replicated such that both the parental cell and daughter cell receive a copy of DNA after division. (b) You have created an ...
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Ch13DNA08 - ChemistryVCE

... ability to produce an identical replica molecule. By alternately heating and cooling the sample containing DNA. PCR has enabled forensic scientists to work with extremely small samples of DNA. PCR is used to duplicate DNA fragments: 1.Denaturation: the sample is heated to 95 C for 1 to 5 minutes. Hy ...
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Lecture 10: Nucleic acids (DNA & RNA)
Lecture 10: Nucleic acids (DNA & RNA)

... most organisms (humans, animals, bacteria, plants, and some viruses). 2) Ribonucleic acid (RNA): in some viruses, RNA serves as the genetic material.  Nucleic acids store and transmit genetic information ‫المعلومات الوراثية‬  Organisms inherit ‫ ترث‬DNA from their parents.  Each DNA molecule is v ...
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... 15. Explain what DNA polymerase is by breaking the word into its parts. _______________________________________________________________ 16. Write a short analogy to explain what replication is. _______________________________________________________________ ...
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... effect on enzyme activity would you expect under the following conditions: a mutation in the trpR gene, encoding TrpR the tryptophan repressor, such that TrpR can bind DNA without the co-repressor A. constitutive, high-level activity B. no activity in the absence of tryptophan, high-level activity i ...
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Chapter 10 - Power Point Presentation

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PowerPoint Notes on Chapter 9 - DNA: The Genetic Material (Video

... Summarize the process of DNA replication. Describe how errors are corrected during DNA replication. Compare the number of replication forks in prokaryotic and eukaryotic DNA. Roles of Enzymes in DNA Replication The complementary structure of DNA is used as a basis to make exact copies of the DNA eac ...
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... A. Glycolysis is the breakdown of one molecule of glucose into two molecules of pyruvate. B. Glycolysis occurs in the ...
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Replisome



The replisome is a complex molecular machine that carries out replication of DNA. The replisome first unwinds double stranded DNA into two single strands. For each of the resulting single strands, a new complementary sequence of DNA is synthesized. The net result is formation of two new double stranded DNA sequences that are exact copies of the original double stranded DNA sequence.In terms of structure, the replisome is composed of two replicative polymerase complexes, one of which synthesizes the leading strand, while the other synthesizes the lagging strand. The replisome is composed of a number of proteins including helicase, RFC, PCNA, gyrase/topoisomerase, SSB/RPA, primase, DNA polymerase I, RNAse H, and ligase.
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