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Brooker Chapter 10
Brooker Chapter 10

... • Introduces negative supercoils using energy from ATP • It can also relax positive supercoils when they occur ...
Heredity and Genes
Heredity and Genes

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Replication - University of Idaho
Replication - University of Idaho

... Origins of Replication Site where replication begins on a circular or linear piece of DNA. Site is rich in A-T base pairs to facilitate separation of the DNA strands. With circular DNA (prokaryotes), there is usually a single origin of replication. ...
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N6-(6-Aminohexyl)dATP - Thermo Fisher Scientific

... 125 µl of 100 mM Tris-HC1 (pH 7.5), 0.1 mM EDTA. It is a dATP analog that contains a primary amino group attached via a 6-carbon linker at the N6position of the purine base1. AHdATP can be incorporated into DNA by nick translation in the presence of dTTP, dGTP, and dCTP. Life Technologies recommends ...
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... 5. Which statement describes a cell process that is common to both eukaryotic and prokaryotic cells? A. Both cell types carry out transcription in the nucleus. B. Both cell types use ribosomes to carry out translation. C. Both cell types assemble amino acids to carry out transcription. D. Both cell ...
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Replisome



The replisome is a complex molecular machine that carries out replication of DNA. The replisome first unwinds double stranded DNA into two single strands. For each of the resulting single strands, a new complementary sequence of DNA is synthesized. The net result is formation of two new double stranded DNA sequences that are exact copies of the original double stranded DNA sequence.In terms of structure, the replisome is composed of two replicative polymerase complexes, one of which synthesizes the leading strand, while the other synthesizes the lagging strand. The replisome is composed of a number of proteins including helicase, RFC, PCNA, gyrase/topoisomerase, SSB/RPA, primase, DNA polymerase I, RNAse H, and ligase.
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