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GeneMATRIX PCR / DNA Clean-Up Purification Kit
GeneMATRIX PCR / DNA Clean-Up Purification Kit

Chapter 20: Biotechnology AP Biology Reading Guide Overview 1
Chapter 20: Biotechnology AP Biology Reading Guide Overview 1

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Chapter 09 Lecture PowerPoint - McGraw Hill Higher Education

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A document that can help for writing your lab report: www
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... and produced via DNA cloning • Add radioactive phosphorus to DNA • Divide DNA into 4 groups that undergo different chemical treatments that break DNA into pieces • DNA pieces are separated by gel electrophoresis and banding patterns are analyzed to reveal sequence of bases ...
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Replisome



The replisome is a complex molecular machine that carries out replication of DNA. The replisome first unwinds double stranded DNA into two single strands. For each of the resulting single strands, a new complementary sequence of DNA is synthesized. The net result is formation of two new double stranded DNA sequences that are exact copies of the original double stranded DNA sequence.In terms of structure, the replisome is composed of two replicative polymerase complexes, one of which synthesizes the leading strand, while the other synthesizes the lagging strand. The replisome is composed of a number of proteins including helicase, RFC, PCNA, gyrase/topoisomerase, SSB/RPA, primase, DNA polymerase I, RNAse H, and ligase.
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