Bubble dynamics in DNA
... ssDNA-binding T4 gene 32 protein has been probed, and a kinetic barrier for the single-strand binders identified [5]. Denaturation bubbles can also be induced by under-winding the DNA double helix [6]. A recent study of the dynamics of these twist-induced bubbles in a random DNA sequence shows that ...
... ssDNA-binding T4 gene 32 protein has been probed, and a kinetic barrier for the single-strand binders identified [5]. Denaturation bubbles can also be induced by under-winding the DNA double helix [6]. A recent study of the dynamics of these twist-induced bubbles in a random DNA sequence shows that ...
DSHIFT: a web server for predicting DNA chemical shifts
... if residual structures are present in DNA sequences. Higher temperatures tend to eliminate residual structures. Since temperature coefficients of random coil chemical shifts have been found to be quite small and they are in the same order of magnitudes as the measurement uncertainties (1–3), the predi ...
... if residual structures are present in DNA sequences. Higher temperatures tend to eliminate residual structures. Since temperature coefficients of random coil chemical shifts have been found to be quite small and they are in the same order of magnitudes as the measurement uncertainties (1–3), the predi ...
51 - Lab Times
... might say but where is the connection to epigenetic research? The answer is quite obvious, if you imagine what happens to symmetrical modified sites, such as fully methylated CpG sites during MspJI restriction. Actually, MspJI cleaves on both 3’ sides and cuts out a DNA fragment with a fixed length ...
... might say but where is the connection to epigenetic research? The answer is quite obvious, if you imagine what happens to symmetrical modified sites, such as fully methylated CpG sites during MspJI restriction. Actually, MspJI cleaves on both 3’ sides and cuts out a DNA fragment with a fixed length ...
Recent Advances in Developing Small Molecules Targeting Nucleic
... Nucleic acids play significant roles in variety kinds of biological processes [1–5]. According to the differences on sugar scaffold, nucleic acids can be classified into two categories. DNAs and RNAs participate in gene storage, replication, transcription and other important biological activities. T ...
... Nucleic acids play significant roles in variety kinds of biological processes [1–5]. According to the differences on sugar scaffold, nucleic acids can be classified into two categories. DNAs and RNAs participate in gene storage, replication, transcription and other important biological activities. T ...
Two-Dimensional DNA Gel Electrophoresis Mapping: a Novel
... DNA extraction and PCR amplification DNA extraction from an environmental sample is important in almost all molecular-based methods. Thus, the yield and purification of DNA are always recommended for capturing the entire repertoire of environmental bacteria. In this study, we used mechanical lysis b ...
... DNA extraction and PCR amplification DNA extraction from an environmental sample is important in almost all molecular-based methods. Thus, the yield and purification of DNA are always recommended for capturing the entire repertoire of environmental bacteria. In this study, we used mechanical lysis b ...
R - Genetics
... either with the specific DNA (from a capsule-deficient strain) responsible for their production or with the DNA extracted from cells of their own strain (clone). This finding suggests genotypic identity of the capsule-deficient transformants with the capsule-deficient donor cells. The spontaneous ca ...
... either with the specific DNA (from a capsule-deficient strain) responsible for their production or with the DNA extracted from cells of their own strain (clone). This finding suggests genotypic identity of the capsule-deficient transformants with the capsule-deficient donor cells. The spontaneous ca ...
light - Microbiology
... Ultraviolet irradiation of Escherichia coli K 12 bacteria, carrying an autonomous F factor or an F-prime factor, enhances the fertility of the population by increasing the number of cells which can transfer the bacterial chromosome. In contrast, under similar conditions the fertility of irradiated H ...
... Ultraviolet irradiation of Escherichia coli K 12 bacteria, carrying an autonomous F factor or an F-prime factor, enhances the fertility of the population by increasing the number of cells which can transfer the bacterial chromosome. In contrast, under similar conditions the fertility of irradiated H ...
Read the Nobel Lecture
... First we needed an assay – a way to detect if telomere elongation was happening. The first assay we tried explored whether a piece of DNA that included a telomere would incorporate DNA precursors more readily than a piece of DNA containing non-telomeric sequences. The idea was that if there was an ...
... First we needed an assay – a way to detect if telomere elongation was happening. The first assay we tried explored whether a piece of DNA that included a telomere would incorporate DNA precursors more readily than a piece of DNA containing non-telomeric sequences. The idea was that if there was an ...
Ultraviolet Induction of Chromosome Transfer by
... Ultraviolet irradiation of Escherichia coli K 12 bacteria, carrying an autonomous F factor or an F-prime factor, enhances the fertility of the population by increasing the number of cells which can transfer the bacterial chromosome. In contrast, under similar conditions the fertility of irradiated H ...
... Ultraviolet irradiation of Escherichia coli K 12 bacteria, carrying an autonomous F factor or an F-prime factor, enhances the fertility of the population by increasing the number of cells which can transfer the bacterial chromosome. In contrast, under similar conditions the fertility of irradiated H ...
The splicing factor SR45 affects the RNA
... pathway.18 This suggests SR45, and perhaps other spliceosome factors, may indeed play a direct role in siRNA accumulation as well. We screened a number of known or putative spliceosome factors as part of our screen; however, sr45 was the only one with Figure 2. Analysis of the FWA transgene methylat ...
... pathway.18 This suggests SR45, and perhaps other spliceosome factors, may indeed play a direct role in siRNA accumulation as well. We screened a number of known or putative spliceosome factors as part of our screen; however, sr45 was the only one with Figure 2. Analysis of the FWA transgene methylat ...
Isolation of a Transforming Sequence from a Human Bladder
... These tertiary foci were picked and grown into mass cultures. The DNAs of these cultures were cleaved by endonuclease Pvu II prior to Southern blot analysis as above. (Lane a) DNA of secondary focus EJ-6-2. (Lanes b-f) DNAs of tertiary foci, termed EJ-6-2$W5. EJ-62-(Rl)4, EJ-6-2-
... These tertiary foci were picked and grown into mass cultures. The DNAs of these cultures were cleaved by endonuclease Pvu II prior to Southern blot analysis as above. (Lane a) DNA of secondary focus EJ-6-2. (Lanes b-f) DNAs of tertiary foci, termed EJ-6-2$W5. EJ-62-(Rl)4, EJ-6-2-
Protamine-mediated DNA coating remarkably improves
... co-delivered transgenes in the cell (Torney et al. 2007). Various devices for accelerating small particles (microprojectiles) have been designed and reported to successfully deliver DNA into plant cells (Christou et al. 1991; Finer et al. 1992; Klein et al. 1987; McCabe and Christou 1993; Sanford et ...
... co-delivered transgenes in the cell (Torney et al. 2007). Various devices for accelerating small particles (microprojectiles) have been designed and reported to successfully deliver DNA into plant cells (Christou et al. 1991; Finer et al. 1992; Klein et al. 1987; McCabe and Christou 1993; Sanford et ...
BIOLOGY (Theory) 57/2 SECTION – A 1. Name the two gases
... 7. State one reason why breast-feeding the baby acts as a natural contraceptive for the mother. Ans. Breast-feeding prevents ovulation during lactation/absence of menstruation ...
... 7. State one reason why breast-feeding the baby acts as a natural contraceptive for the mother. Ans. Breast-feeding prevents ovulation during lactation/absence of menstruation ...
Lecture NoteIV
... In addition to DNA, a cell extract contains significant quantities of protein and RNA which can be further purified by following methods4-1.2.3.1. Organic extraction and enzymatic digestion for the removal of contaminants It involves the addition of a mixture of phenol and chloroform (1:1) to the ce ...
... In addition to DNA, a cell extract contains significant quantities of protein and RNA which can be further purified by following methods4-1.2.3.1. Organic extraction and enzymatic digestion for the removal of contaminants It involves the addition of a mixture of phenol and chloroform (1:1) to the ce ...
Chapter 28. Heterocycles and Nucleic Acids
... amino acid residues are to be joined Codons are sequences of three ribonucleotides that specify a particular amino acid For example, UUC on mRNA is a codon that directs incorporation of phenylalanine into the growing protein Based on McMurry, Organic Chemistry, Chapter 28, 6th edition, (c) 2003 ...
... amino acid residues are to be joined Codons are sequences of three ribonucleotides that specify a particular amino acid For example, UUC on mRNA is a codon that directs incorporation of phenylalanine into the growing protein Based on McMurry, Organic Chemistry, Chapter 28, 6th edition, (c) 2003 ...
Slide 1
... Large numbers of identical bacteria, each containing the inserted DNA molecules, can be produced through a process called cloning. ...
... Large numbers of identical bacteria, each containing the inserted DNA molecules, can be produced through a process called cloning. ...
qRT-PCR Primer Design Using IDT Primer Quest Dr. Ray Enke Bio
... You now have 2 ApE files that we will use to ID the exon/intron junctions. This is important because the primers we design will span one of these junctions so that only cDNAs and not contaminating genomic DNA will be amplified by our primer sets. II. Annotating mRNA exon junctions in ApE Sequence Ed ...
... You now have 2 ApE files that we will use to ID the exon/intron junctions. This is important because the primers we design will span one of these junctions so that only cDNAs and not contaminating genomic DNA will be amplified by our primer sets. II. Annotating mRNA exon junctions in ApE Sequence Ed ...
Finding Data in DNA: Computer Forensic Investigations of Living
... automatically creates a backup copy of the data contained in its DNA. In addition, selective pressure and DNA error correction reduce the risk of the data being destroyed by random mutations. It has been suggested to use cockroaches, which are known for their resilience and high reproduction rate, a ...
... automatically creates a backup copy of the data contained in its DNA. In addition, selective pressure and DNA error correction reduce the risk of the data being destroyed by random mutations. It has been suggested to use cockroaches, which are known for their resilience and high reproduction rate, a ...
Replisome
The replisome is a complex molecular machine that carries out replication of DNA. The replisome first unwinds double stranded DNA into two single strands. For each of the resulting single strands, a new complementary sequence of DNA is synthesized. The net result is formation of two new double stranded DNA sequences that are exact copies of the original double stranded DNA sequence.In terms of structure, the replisome is composed of two replicative polymerase complexes, one of which synthesizes the leading strand, while the other synthesizes the lagging strand. The replisome is composed of a number of proteins including helicase, RFC, PCNA, gyrase/topoisomerase, SSB/RPA, primase, DNA polymerase I, RNAse H, and ligase.