Visualization of oligonucleotide probes and point mutations in
... ODNs, P1 ‘‘anchor’’ probes, AD-ODNs, circles, and decorator probes for RCA reactions are listed in Tables 1 and 2. The probe-primer ODNs and the AD-P2-ODNs were designed to have two 3⬘ ends. The synthesis of these ODNs was initiated from the 3⬘ end of the primer sequence with reverse amidites and ch ...
... ODNs, P1 ‘‘anchor’’ probes, AD-ODNs, circles, and decorator probes for RCA reactions are listed in Tables 1 and 2. The probe-primer ODNs and the AD-P2-ODNs were designed to have two 3⬘ ends. The synthesis of these ODNs was initiated from the 3⬘ end of the primer sequence with reverse amidites and ch ...
Replication timing and transcriptional control: beyond
... assays for replication [29]. There are only two mammalian loci for which both replication-timing switches and origin localization have been studied. At the mouse immunoglobulin IgH locus, new origins are activated in pre-B cells when the promoter region of this locus becomes transcriptionally active ...
... assays for replication [29]. There are only two mammalian loci for which both replication-timing switches and origin localization have been studied. At the mouse immunoglobulin IgH locus, new origins are activated in pre-B cells when the promoter region of this locus becomes transcriptionally active ...
Chromosome mapping of the sweet potato little leaf
... To further understand the genomic diversity and genetic architecture of phytoplasmas, a physical and genetic map of the sweet potato little leaf (SPLL) strain V4 phytoplasma chromosome was determined. PFGE was used to determine the size of the SPLL-V4 genome, which was estimated to be 622 kb. A phys ...
... To further understand the genomic diversity and genetic architecture of phytoplasmas, a physical and genetic map of the sweet potato little leaf (SPLL) strain V4 phytoplasma chromosome was determined. PFGE was used to determine the size of the SPLL-V4 genome, which was estimated to be 622 kb. A phys ...
CHAPTER 5 Gene Expression: Transcription
... • 1. Transcription is divided into three steps for both prokaryotes and eukaryotes. They are initiation, elongation and termination. The process of elongation is highly conserved between prokaryotes and eukaryotes, but initiation and termination are somewhat different. ...
... • 1. Transcription is divided into three steps for both prokaryotes and eukaryotes. They are initiation, elongation and termination. The process of elongation is highly conserved between prokaryotes and eukaryotes, but initiation and termination are somewhat different. ...
Lecture-Mic 623-Plasmids-Corynebacterium - Home
... involved in the utilization of haemin and haemoglobin as iron sources by a Gram- ...
... involved in the utilization of haemin and haemoglobin as iron sources by a Gram- ...
Transcription - Shippensburg University
... • Transcription factors mediate the binding of RNA polymerase and the initiation of transcription • The completed assembly of transcription factors and RNA polymerase II bound to a promoter is called a transcription initiation complex • A promoter called a TATA box is crucial in forming the initiati ...
... • Transcription factors mediate the binding of RNA polymerase and the initiation of transcription • The completed assembly of transcription factors and RNA polymerase II bound to a promoter is called a transcription initiation complex • A promoter called a TATA box is crucial in forming the initiati ...
DNA cloning
... for cleavage of the DNA. The complex also has topoisomerase activity. The DNA sequence recognized by Type I enzymes is also complex. The sequence is asymmetric and split into two parts (Fig. 14.1a). The first part is a 3 bp sequence, next is a 6–8 bp spacer of nonspecific sequence, and finally there ...
... for cleavage of the DNA. The complex also has topoisomerase activity. The DNA sequence recognized by Type I enzymes is also complex. The sequence is asymmetric and split into two parts (Fig. 14.1a). The first part is a 3 bp sequence, next is a 6–8 bp spacer of nonspecific sequence, and finally there ...
RNA and Protein Synthesis Powerpoint
... nucleotides to one side of the DNA molecule/the sense side of DNA. -DNA is a double strand of nucleotides BUT only one side of the DNA molecule actually codes for making proteins. ...
... nucleotides to one side of the DNA molecule/the sense side of DNA. -DNA is a double strand of nucleotides BUT only one side of the DNA molecule actually codes for making proteins. ...
w + gene is silenced in some cells
... Consist of satellite DNAs, which are repetitive, noncoding sequences • Tandem repeats of 5 – 300 bp long, can extend over megabases of DNA • Have different chromatin structure and higher-order packaging than other chromosomal regions • Predominant human satellite DNA is a-satellite 171 bp repeat, ...
... Consist of satellite DNAs, which are repetitive, noncoding sequences • Tandem repeats of 5 – 300 bp long, can extend over megabases of DNA • Have different chromatin structure and higher-order packaging than other chromosomal regions • Predominant human satellite DNA is a-satellite 171 bp repeat, ...
Chapter 22: SV40 and Polyomavirus DNA Replication
... also influenced by the nature and composition of the spacer region between pentanucleotide repeats (DeLucia et al. 1983; Dean et al. 1987; Deb et al. 1987). Large T antigen also binds to single-stranded DNA; this activity may be integral to its ability to unwind DNA at the replication fork (for revi ...
... also influenced by the nature and composition of the spacer region between pentanucleotide repeats (DeLucia et al. 1983; Dean et al. 1987; Deb et al. 1987). Large T antigen also binds to single-stranded DNA; this activity may be integral to its ability to unwind DNA at the replication fork (for revi ...
The effect of human serum DNAases on the ability to detect
... In¯uence of human DNAases on the detection rate of puri®ed E. coli DNA by PCR Twenty samples of serum (0.1 ml) from a healthy individual were each inoculated with 30 pg of puri®ed E. coli DNA (Amersham Pharmacia Biotech, Freiburg, Germany). Native human serum was prepared from venous blood as descri ...
... In¯uence of human DNAases on the detection rate of puri®ed E. coli DNA by PCR Twenty samples of serum (0.1 ml) from a healthy individual were each inoculated with 30 pg of puri®ed E. coli DNA (Amersham Pharmacia Biotech, Freiburg, Germany). Native human serum was prepared from venous blood as descri ...
rna interference
... molecular biology where the presence of certain fragments of double-stranded RNA (dsRNA) interferes with the expression of a particular gene. RNAi appears to be a highly potent and specific process which is actively carried out by special mechanisms in the cell, known as the RNA interference machine ...
... molecular biology where the presence of certain fragments of double-stranded RNA (dsRNA) interferes with the expression of a particular gene. RNAi appears to be a highly potent and specific process which is actively carried out by special mechanisms in the cell, known as the RNA interference machine ...
chromosomes_nice
... A prokaryotic chromosome consists of a single molecule of DNA in the form of a closed loop. The chromosome is described as circular. A prokaryotic cell has only one chromosome. A eukaryotic chromosome is linear, not circular, in other words it has two ends, like a sausage. Each chromosome contains ...
... A prokaryotic chromosome consists of a single molecule of DNA in the form of a closed loop. The chromosome is described as circular. A prokaryotic cell has only one chromosome. A eukaryotic chromosome is linear, not circular, in other words it has two ends, like a sausage. Each chromosome contains ...
LECTURE 1 Human Chromosomes Human Karyotype
... By the end of this lecture, the students should be able to: Describe the number, structure, and classification of human chromosomes. Explain what a Karyotype is and how it is obtained. Describe chromosomal banding and explain its use. Describe the process of in situ hybridization and the infor ...
... By the end of this lecture, the students should be able to: Describe the number, structure, and classification of human chromosomes. Explain what a Karyotype is and how it is obtained. Describe chromosomal banding and explain its use. Describe the process of in situ hybridization and the infor ...
Replisome
The replisome is a complex molecular machine that carries out replication of DNA. The replisome first unwinds double stranded DNA into two single strands. For each of the resulting single strands, a new complementary sequence of DNA is synthesized. The net result is formation of two new double stranded DNA sequences that are exact copies of the original double stranded DNA sequence.In terms of structure, the replisome is composed of two replicative polymerase complexes, one of which synthesizes the leading strand, while the other synthesizes the lagging strand. The replisome is composed of a number of proteins including helicase, RFC, PCNA, gyrase/topoisomerase, SSB/RPA, primase, DNA polymerase I, RNAse H, and ligase.