Application of a Real Time Polymerase Chain Reaction Method to
... a better choice if the processing time is critical. The DNA Stool method took half the time for DNA extraction, and the increase in Ct value was only 1-2 cycles. For spiked egg samples, the Ct values from the CTAB method were 23 for the ricin and 18S rRNA genes, but the Ct values for both ricin and ...
... a better choice if the processing time is critical. The DNA Stool method took half the time for DNA extraction, and the increase in Ct value was only 1-2 cycles. For spiked egg samples, the Ct values from the CTAB method were 23 for the ricin and 18S rRNA genes, but the Ct values for both ricin and ...
PowerPoint 演示文稿
... The terminator of the ρ dependent termination is not typical. It contains CA-rich region which ρ factor can recognize. ρ factor is a homo-hexamer protein factor , can bind the newly synthesized RNA and moves to the RNA-DNA hybrid region. ρ factor has helicase activity and unwinds the RNA-DNA helix d ...
... The terminator of the ρ dependent termination is not typical. It contains CA-rich region which ρ factor can recognize. ρ factor is a homo-hexamer protein factor , can bind the newly synthesized RNA and moves to the RNA-DNA hybrid region. ρ factor has helicase activity and unwinds the RNA-DNA helix d ...
Protein–Nucleic Acid Interactions
... 10.1a). The major groove of the A-form is comparatively narrower than the B-form, so that the bases of the Aform are more deeply buried within the body of the duplex and are consequently less accessible from that side. In contrast, the minor groove of the A-form is widened and flattened relative to ...
... 10.1a). The major groove of the A-form is comparatively narrower than the B-form, so that the bases of the Aform are more deeply buried within the body of the duplex and are consequently less accessible from that side. In contrast, the minor groove of the A-form is widened and flattened relative to ...
Structure, stability and function of 5-chlorouracil
... base pair geometries of ClU with A and G in more detail. To date, no crystallographic study of a DNA duplex comprising ClU:A or ClU:G base pairs has been reported. A nuclear magnetic resonance (NMR) investigation by Theruvathu et al. (14) did not bring to light any substantial difference in the geom ...
... base pair geometries of ClU with A and G in more detail. To date, no crystallographic study of a DNA duplex comprising ClU:A or ClU:G base pairs has been reported. A nuclear magnetic resonance (NMR) investigation by Theruvathu et al. (14) did not bring to light any substantial difference in the geom ...
REAL-TIME PCR
... fluorophore emits light of a longer wavelength, which is detected by the real-time PCR system at a channel specific for that wavelength. The first dye, linked to the donor probe, is a fluorescent dye with an excitation peak of a shorter-wavelength (~ 480-530 nm) while the second, linked to the accep ...
... fluorophore emits light of a longer wavelength, which is detected by the real-time PCR system at a channel specific for that wavelength. The first dye, linked to the donor probe, is a fluorescent dye with an excitation peak of a shorter-wavelength (~ 480-530 nm) while the second, linked to the accep ...
Chapter 2: Introduction to Molecular Genetics
... This structure refers to how DNA is stored in a confined space to form the chromosomes. It varies depending on whether the organisms prokaryotes and eukaryotes: - In prokaryotes the DNA is folded like a super-helix, usually in circular shape and associated with a small amount of protein. The sam ...
... This structure refers to how DNA is stored in a confined space to form the chromosomes. It varies depending on whether the organisms prokaryotes and eukaryotes: - In prokaryotes the DNA is folded like a super-helix, usually in circular shape and associated with a small amount of protein. The sam ...
Construction of recombinant plasmid carrying the λ DNA fragment
... cells is one of the major problems of recombinant DNA research. The stabilization of foreign DNA sequences in the cell can be achieved by integration of these sequences into host chromosome. It may be carried out by means of A vectors containing the genes necessary for stable lysogenization. However ...
... cells is one of the major problems of recombinant DNA research. The stabilization of foreign DNA sequences in the cell can be achieved by integration of these sequences into host chromosome. It may be carried out by means of A vectors containing the genes necessary for stable lysogenization. However ...
TNT SP6 High-Yield Wheat Germ Protein Expression System
... Flexi® Vectors (Cat.# L5671 and L5681, respectively) are designed for expression of proteins in wheat germ extract. These vectors incorporate sequences from the barley yellow dwarf virus (BYDV) upstream and downstream of the protein-coding region of interest. The vectors contain SgfI and PmeI sites ...
... Flexi® Vectors (Cat.# L5671 and L5681, respectively) are designed for expression of proteins in wheat germ extract. These vectors incorporate sequences from the barley yellow dwarf virus (BYDV) upstream and downstream of the protein-coding region of interest. The vectors contain SgfI and PmeI sites ...
To Know Ourselves
... helix—held together by weak bonds between complementary bases, adenine (A) in one strand to thymine (T) in the other, and cytosine to guanine (C–G). In the language of molecular genetics, each of these linkages constitutes a base pair. All told, if we count only one of each pair of chromosomes, the ...
... helix—held together by weak bonds between complementary bases, adenine (A) in one strand to thymine (T) in the other, and cytosine to guanine (C–G). In the language of molecular genetics, each of these linkages constitutes a base pair. All told, if we count only one of each pair of chromosomes, the ...
Uniikki kuitu
... Proteins or protein domains belonging to a particular family generally share functional attributes and are derived from a common ancestor.It is apparent, when studying protein sequence families, that some regions have been better conserved than others during evolution. ...
... Proteins or protein domains belonging to a particular family generally share functional attributes and are derived from a common ancestor.It is apparent, when studying protein sequence families, that some regions have been better conserved than others during evolution. ...
Detection of DNA molecules by SERS spectroscopy with silvered
... guanine, adenine), 1490 cm1 (guanine, adenine), and 1581 cm1 (guanine, adenine) are the characteristic Raman bands of the herring sperm DNA molecules [10]. SERS spectra were collected in 10 random points of the silvered PS. Remarkably, the silvered PS demonstrates SERS activity for all lasers prov ...
... guanine, adenine), 1490 cm1 (guanine, adenine), and 1581 cm1 (guanine, adenine) are the characteristic Raman bands of the herring sperm DNA molecules [10]. SERS spectra were collected in 10 random points of the silvered PS. Remarkably, the silvered PS demonstrates SERS activity for all lasers prov ...
Disrupting antibiotic resistance propagation by inhibiting
... We then considered models for conjugative DNA transfer that would require the observed 2⫹ metal ion and two catalytically competent tyrosine residues (Fig. 1B). In the case of simple transfer (Fig. 1B, green), a single catalytic tyrosine is sufficient for successful intercellular DNA transfer. Howev ...
... We then considered models for conjugative DNA transfer that would require the observed 2⫹ metal ion and two catalytically competent tyrosine residues (Fig. 1B). In the case of simple transfer (Fig. 1B, green), a single catalytic tyrosine is sufficient for successful intercellular DNA transfer. Howev ...
Characterization of DNA Primary Sequences Based on the Average
... information on nucleic acid bases separated by different distances.17 In this paper we will consider the distances between pairs of nucleic acid bases rather than the frequency of the various pairs. We will show how the average distances between pairs of bases lead to a set of novel numerical invari ...
... information on nucleic acid bases separated by different distances.17 In this paper we will consider the distances between pairs of nucleic acid bases rather than the frequency of the various pairs. We will show how the average distances between pairs of bases lead to a set of novel numerical invari ...
Replisome
The replisome is a complex molecular machine that carries out replication of DNA. The replisome first unwinds double stranded DNA into two single strands. For each of the resulting single strands, a new complementary sequence of DNA is synthesized. The net result is formation of two new double stranded DNA sequences that are exact copies of the original double stranded DNA sequence.In terms of structure, the replisome is composed of two replicative polymerase complexes, one of which synthesizes the leading strand, while the other synthesizes the lagging strand. The replisome is composed of a number of proteins including helicase, RFC, PCNA, gyrase/topoisomerase, SSB/RPA, primase, DNA polymerase I, RNAse H, and ligase.