Glossary Excerpted with modification from the Glossary in Genes V
... RNA polymerase. Attenuation describes the regulation of termination of transcription that is involved in controlling the expression of some bacterial operons. Attenuator is the terminator sequence at which attenuation occurs. Autoradiography detects radioactively labeled molecules by their effect in ...
... RNA polymerase. Attenuation describes the regulation of termination of transcription that is involved in controlling the expression of some bacterial operons. Attenuator is the terminator sequence at which attenuation occurs. Autoradiography detects radioactively labeled molecules by their effect in ...
The amount of DNA, # of genes and DNA per gene in various
... nuclease cutting sequence at a particular site creates DNA fragments of different sizes. In a homozygous organism the fragments are of the same size. In a heterozygous organism, the fragments are of different sizes. ...
... nuclease cutting sequence at a particular site creates DNA fragments of different sizes. In a homozygous organism the fragments are of the same size. In a heterozygous organism, the fragments are of different sizes. ...
Chapter 8: Cell Division
... 3. Understand the concepts of: semi-conservative replication. 4. Understand leading and lagging strand synthesis in DNA replication (and that lagging strand synthesis is due to the nature of DNA polymerase in that it only works in the 5’ to 3’ direction). 5. Understand the concept of DNA proofreadin ...
... 3. Understand the concepts of: semi-conservative replication. 4. Understand leading and lagging strand synthesis in DNA replication (and that lagging strand synthesis is due to the nature of DNA polymerase in that it only works in the 5’ to 3’ direction). 5. Understand the concept of DNA proofreadin ...
M0290Datasheet-Lot0601204
... the 5´ phosphoryl termini required by ligases, they cannot self-ligate (1). This property can be used to decrease the vector background in cloning strategies. Source: Calf intestinal mucosa Molecular Weight: 69 kDa ...
... the 5´ phosphoryl termini required by ligases, they cannot self-ligate (1). This property can be used to decrease the vector background in cloning strategies. Source: Calf intestinal mucosa Molecular Weight: 69 kDa ...
Southern Transfer
... To determine the copy number of Myb transcription factor genes in the genome of the model plant Arabidopsis thaliana ...
... To determine the copy number of Myb transcription factor genes in the genome of the model plant Arabidopsis thaliana ...
DNA - The Double Helix
... "unzip" when it needs to replicate - or make a copy of itself. DNA needs to copy itself when a cell divides, so that the new cells each contain a copy of the DNA. Without these instructions, the new cells wouldn't have the correct information. The hydrogen bonds are represented by small circles. Col ...
... "unzip" when it needs to replicate - or make a copy of itself. DNA needs to copy itself when a cell divides, so that the new cells each contain a copy of the DNA. Without these instructions, the new cells wouldn't have the correct information. The hydrogen bonds are represented by small circles. Col ...
Some Products Made Using Biotechnology
... c.) Combine the “sticky ends” of the two DNA pieces together with DNA ligase (enzyme) – also known as gene splicing d.) Insert vector into bacteria. e.) The bacteria can now reproduce the recombinant DNA and the foreign genes will be expressed in the bacteria. ...
... c.) Combine the “sticky ends” of the two DNA pieces together with DNA ligase (enzyme) – also known as gene splicing d.) Insert vector into bacteria. e.) The bacteria can now reproduce the recombinant DNA and the foreign genes will be expressed in the bacteria. ...
DNA - The Double Helix
... hydrogen bonds. The DNA can actually "unzip" when it needs to replicate or make a copy of itself. DNA needs to copy itself when a cell divides, so that the new cells each contain a copy of the DNA. Without these instructions, the new cells wouldn't have the correct information. The hydrogen bonds ar ...
... hydrogen bonds. The DNA can actually "unzip" when it needs to replicate or make a copy of itself. DNA needs to copy itself when a cell divides, so that the new cells each contain a copy of the DNA. Without these instructions, the new cells wouldn't have the correct information. The hydrogen bonds ar ...
heredity (b)
... The monomer is made of what parts? DNA replication is crucial to the advancement of a cell in the cell cycle. In what stage does a cells chromosomal DNA go through replication? What is the principal enzyme that is responsible for DNA replication? DNA replication is directional. In what direction doe ...
... The monomer is made of what parts? DNA replication is crucial to the advancement of a cell in the cell cycle. In what stage does a cells chromosomal DNA go through replication? What is the principal enzyme that is responsible for DNA replication? DNA replication is directional. In what direction doe ...
DNA - The Double Helix
... hydrogen bonds. The DNA can actually "unzip" when it needs to replicate or make a copy of itself. DNA needs to copy itself when a cell divides, so that the new cells each contain a copy of the DNA. Without these instructions, the new cells wouldn't have the correct information. The hydrogen bonds ar ...
... hydrogen bonds. The DNA can actually "unzip" when it needs to replicate or make a copy of itself. DNA needs to copy itself when a cell divides, so that the new cells each contain a copy of the DNA. Without these instructions, the new cells wouldn't have the correct information. The hydrogen bonds ar ...
Molecular biology Tools
... Technique based on antigen-antibody reaction Examples: HIV tests &PGE2 ...
... Technique based on antigen-antibody reaction Examples: HIV tests &PGE2 ...
DNA - Cloudfront.net
... identical DNA organized into linear chromosomes. – Different organisms have different numbers of chromosomes – Humans 46, Drosophila 8, Sequoia Tree 22 • On chromosome 1 of human DNA there are 249 million base pairs coding for approximately 4000 genes. • An average eukaryotic cell (100µm) has over 6 ...
... identical DNA organized into linear chromosomes. – Different organisms have different numbers of chromosomes – Humans 46, Drosophila 8, Sequoia Tree 22 • On chromosome 1 of human DNA there are 249 million base pairs coding for approximately 4000 genes. • An average eukaryotic cell (100µm) has over 6 ...
Slide 1
... many freshwaters, yet substantial numbers of microorganisms exist there. Many of these use light to drive ATP synthesis. In terms of prokaryotes, species of the domain Bacteria tend to predominate in oceanic surface waters whereas Archaea are more prevalent in ...
... many freshwaters, yet substantial numbers of microorganisms exist there. Many of these use light to drive ATP synthesis. In terms of prokaryotes, species of the domain Bacteria tend to predominate in oceanic surface waters whereas Archaea are more prevalent in ...
Protein Synthesis
... i. Gene = a segment of DNA coding for a RNA segment. These RNA segments will be used to produce a polypeptide (structural or enzymatic protein) ii. Each strand of DNA can contain thousands of genes iii. Each gene has a beginning and an end b. DNA is used as the blueprint to direct the production of ...
... i. Gene = a segment of DNA coding for a RNA segment. These RNA segments will be used to produce a polypeptide (structural or enzymatic protein) ii. Each strand of DNA can contain thousands of genes iii. Each gene has a beginning and an end b. DNA is used as the blueprint to direct the production of ...
Chapter 1 – The nature of science
... The difference between eukaryotes and prokaryotes. Structure and function of the following cellular organelles o Mitochondria o Chloroplast o Ribosomes o Rough endoplasmic reticulum o Smooth endoplasmic reticulum o Golgi apparatus o Lysosomes o Vacuoles o Plastids o Cytoskeleton o Cilia and flag ...
... The difference between eukaryotes and prokaryotes. Structure and function of the following cellular organelles o Mitochondria o Chloroplast o Ribosomes o Rough endoplasmic reticulum o Smooth endoplasmic reticulum o Golgi apparatus o Lysosomes o Vacuoles o Plastids o Cytoskeleton o Cilia and flag ...
Chapter 16
... is absent. If operator is bound, promoter region is partially blocked-genes can not be transcribed. • This two switch control mechanism thus causes the cell to produce only what the cell needs, when it needs it. ...
... is absent. If operator is bound, promoter region is partially blocked-genes can not be transcribed. • This two switch control mechanism thus causes the cell to produce only what the cell needs, when it needs it. ...
BACTERIAL GENETICS
... from one type of bacteria and inject it into genetically different bacteria. When purified DNA is injected into the nucleus of a eukaryotic cell, the process is called transfection. Transfection is frequently used in genetic engineering procedures ...
... from one type of bacteria and inject it into genetically different bacteria. When purified DNA is injected into the nucleus of a eukaryotic cell, the process is called transfection. Transfection is frequently used in genetic engineering procedures ...
Molecular Pathology - Fahd Al
... • A PCR (Polymerase Chain Reaction) is performed in order to make a large number of copies of a gene. Otherwise, the quantity of DNA is insufficient and cannot be used for other methods such as sequencing. • A PCR is performed on an automated cycler, which heats and cools the tubes with the reaction ...
... • A PCR (Polymerase Chain Reaction) is performed in order to make a large number of copies of a gene. Otherwise, the quantity of DNA is insufficient and cannot be used for other methods such as sequencing. • A PCR is performed on an automated cycler, which heats and cools the tubes with the reaction ...
Introduction to DNA Function and transcription
... • Where is protein made in a cell? • How does the information stored in DNA determine which proteins can be made in a cell? • How is protein made in a cell? • How does the environment influence which proteins are made in cell? ...
... • Where is protein made in a cell? • How does the information stored in DNA determine which proteins can be made in a cell? • How is protein made in a cell? • How does the environment influence which proteins are made in cell? ...
Replisome
The replisome is a complex molecular machine that carries out replication of DNA. The replisome first unwinds double stranded DNA into two single strands. For each of the resulting single strands, a new complementary sequence of DNA is synthesized. The net result is formation of two new double stranded DNA sequences that are exact copies of the original double stranded DNA sequence.In terms of structure, the replisome is composed of two replicative polymerase complexes, one of which synthesizes the leading strand, while the other synthesizes the lagging strand. The replisome is composed of a number of proteins including helicase, RFC, PCNA, gyrase/topoisomerase, SSB/RPA, primase, DNA polymerase I, RNAse H, and ligase.