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Ch. 13.1: BIOTECHNOLOGY
Ch. 13.1: BIOTECHNOLOGY

... they h-bond w/ complimentary bases in other fragments. Helps to “sew” fragments together & make recomb. DNA. ...
Study guide for exam 2 Spring 2017
Study guide for exam 2 Spring 2017

... What are the major phases of the cell cycle? What happens during those stages? Remember the importance of the S-phase. Be familiar with the major stages of mitosis. What occurs during each of these stages? How many daughter cells result from mitosis? Understand the significance of mitosis. How does ...
Something`s Fishy
Something`s Fishy

... guanine, and cytosine. This sequence of A, T, G, and C is unique to each individual. Restriction enzymes cut DNA. Each restriction enzyme recognizes a specific group of “target” base pairs and makes a cut within this area. The resulting fragments are called restriction fragment length polymorphisms ...
Determination of the pH Scale by the Method of
Determination of the pH Scale by the Method of

... common binding mode, in which the molecule sticks into a groove of DNA. The binding is especially interesting if it is “sequence specific”, such that the molecule binds only to specific sequences of DNA base pairs. Such molecules can be used as diagnostics, to indicate if a certain type of DNA is pr ...
Controlling the Code: molecules at work
Controlling the Code: molecules at work

... then that the repressor is released from the operator and no longer blocks the attachment of RNA polymerase to the promoter. This allows transcription to begin. ...
Biotech 06
Biotech 06

CP Final Exam Study Guide 2015KEY
CP Final Exam Study Guide 2015KEY

... 2. What technology led to the discovery of cells? Microscope 3. What is the difference between an inference, hypothesis, and theory? 4. What are independent and dependent variables? (Explain) Independent variables include the factor that is changed in an experiment. Dependent variables change based ...
06. Nucleic acids
06. Nucleic acids

Plasmid w/ kanamycin resistance (pKAN)
Plasmid w/ kanamycin resistance (pKAN)

... let’s predict the A+ fragments ...
Introduction Presentation
Introduction Presentation

... Coding (Genes) versus Non-Coding DNA • However, only a very small % of the genome is actually made up of genes (segments of DNA that directly code for the amino acids plus adjacent segments that influence gene expression) • Most of the genome (approx. 98%) DNA is noncoding; this DNA was presumed to ...
DNA Conductivity: Our Most Recent Results
DNA Conductivity: Our Most Recent Results

DNA, RNA, and Protein Synthesis Note Packet
DNA, RNA, and Protein Synthesis Note Packet

... 2. Messenger RNA is the _____________________ between the DNA in the nucleus and the ribosomes in the cytoplasm. 3. The instructions are carried in the form of _____________. The first codon is called the __________________. This is the point at which mRNA will attach to the ribosome. This tells the ...
DNA Knex modelling lab (1)
DNA Knex modelling lab (1)

... help of Rosalind Franklin, Maurice Wilkens and Erwin Chargraff. The shape of DNA is a double stranded helix, which is like a twisted ladder. The two strands run antiparallel to one another, or in opposite directions. The sides of the ladder are made of alternating deoxyribose sugar and phosphate mol ...
Transduction
Transduction

... Figure 32 : Generalized transduction [ Dale and Park ,2004] ...
Chapter 17 Notes File
Chapter 17 Notes File

... only (New DNA)! In order for DNA polymerase to know where to begin, a small piece of RNA Primase called a primer shows it where to begin. Once begun, the addition of nucleotides continues at a steady pace along the leading strand. ...
View PDF
View PDF

... DNA and the Genetic Code The DNA molecule takes the shape of a double-stranded spiral, which, as you can see from the diagram, looks something like a twisted ladder. In Chapter 2, you read about different subunits that make up the molecules found in cells. Nucleotide subunits make up each of the two ...
Gene Linkage
Gene Linkage

... • Selective Breeding: allowing only those organisms with desired characteristics to reproduce. – How could you use selective breeding to develop dogs with more intelligence? ...
dna adducts - dr
dna adducts - dr

... either exogenous (xenobiotic) or endogenous (including metabolites of xenobiotics). The effect of an adduct depends on it’s location on DNA: • An adduct on a gene will usually reduces or blocks that gene’s expression. • An adduct near (on the promotor region of) a gene may lead to over-expression of ...
DNA: The stuff of Inheritance
DNA: The stuff of Inheritance

... Chargaff analyzed the DNA from a number of different organisms and found that the composition of DNA differs from species to species, thus showing molecular diversity. He also found that in the DNA of a species, adenine and thymine always appear in equal amounts and cytosine and guanine also appear ...
TOPIC 4: GENETICS - Doctor Golub`s Living Environment
TOPIC 4: GENETICS - Doctor Golub`s Living Environment

... The flounder is a species of fish that can live in very cold water. The fish produces an “antifreeze” protein that prevents ice crystals from forming in its blood. The DNA for this protein has been identified. An enzyme is used to cut and remove this section of flounder DNA that is then spliced into ...
DNA EXTRACTION
DNA EXTRACTION

Review sheet for test B5 – B8
Review sheet for test B5 – B8

... 46. Which of the following is not true about RNA? a) RNA transfers messages from DNA to ribosomes b) RNA contains the sugar ribose c) RNA forms a double helix d) RNA is single stranded 47. Prior to protein synthesis, the DNA a) Attracts tRNA’s with appropriate amino acids b) Serves as a template fo ...
Chapter 20 Notes
Chapter 20 Notes

... gene into a bacterial plasmid?  Introns are not spliced in prokaryotes  How can this problem be solved?  Reverse Transcription of mRNA ...
Plasmid w/ kanamycin resistance (pKAN)
Plasmid w/ kanamycin resistance (pKAN)

direct genetic testing
direct genetic testing

... Electropherogram of a second-generation multiplex ‘SGM Plus’ profile from a male ...
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Replisome



The replisome is a complex molecular machine that carries out replication of DNA. The replisome first unwinds double stranded DNA into two single strands. For each of the resulting single strands, a new complementary sequence of DNA is synthesized. The net result is formation of two new double stranded DNA sequences that are exact copies of the original double stranded DNA sequence.In terms of structure, the replisome is composed of two replicative polymerase complexes, one of which synthesizes the leading strand, while the other synthesizes the lagging strand. The replisome is composed of a number of proteins including helicase, RFC, PCNA, gyrase/topoisomerase, SSB/RPA, primase, DNA polymerase I, RNAse H, and ligase.
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