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MF011_fhs_lnt_008b_May10 - mf011
MF011_fhs_lnt_008b_May10 - mf011

... “Slide Sorter” views. All animations will appear after viewing in Presentation Mode and playing each animation. Most animations will require the latest version of the Flash Player, which is available at http://get.adobe.com/flashplayer. ...
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... none of the genes required for the lambda life cycle. There is also a drug selection gene to maintain bacteria that carry the plasmid. What substrate used in chain termination sequencing causes the chain termination? The dideoxynucleotides How are the four bases distinguished in current fluorescence ...
Synthesis and properties of modified oligonucleotides
Synthesis and properties of modified oligonucleotides

... 5-aza-2’-deoxyctyidine, and therefore it is not efficiently incorporated into DNA. In collaboration with Victor Márquez (NIH-National Institutes of Health, USA) and Allen Yang (USC-University of Southern California, USA), we have prepared oligonucleotides carrying 2’-deoxyzebularine in order to meas ...
Understanding DNA
Understanding DNA

... A. mRNA enters the ribosome B. 3 mRNA nucleotides (codons) pair up with 3 tRNA nucleotides (anticodons) C. amino acids are added until the “stop” message is reached ...
DNA MUTATIONS AND THEIR REPAIR
DNA MUTATIONS AND THEIR REPAIR

... very useful during double strand damage. When damage occurs to both DNA strands, the only way that it can be repaired is by homologous recombination using the intact chromosome copy. This allows a damaged chromosome to be replaced, using the sister of the chromosome pair as the template. DNA repair ...
Build-a-Bug - Wando High School
Build-a-Bug - Wando High School

10/14/04 8:25 am
10/14/04 8:25 am

... bonds, not actual covalent bonds. this is so you can unzip the dna during mytosis. Every other bond in the dna is a covalent bond. phosphodiester bonds: COPOC. From theh three carbon, to the oxygen, to the phosphorus, to the oxygen, to the five prime carbon. The two nucleotides that make up one seci ...
Slide
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... • This may lead to the cloning of only part of the gene, as happened for Factor VIII. • Often more work has to be done to piece together the whole gene, introns and exons. • Can you think of a way to take the DNA sequence you have and “go fishing” for all the exons that encode Factor VIII without t ...
Name Class Date RNA and Transcription Make Up #1 RNA Lesson
Name Class Date RNA and Transcription Make Up #1 RNA Lesson

... transcription, segments of DNA serve as templates to produce complementary RNA molecules. In prokaryotes, RNA synthesis and protein synthesis takes place in the cytoplasm. In eukaryotes, RNA is produced in the cell’s nucleus and then moves to the cytoplasm to play a role in the production of protein ...
Build-a-Bug - Wando High School
Build-a-Bug - Wando High School

... During this step, mRNA goes through different types of maturation including one called splicing, when the non-coding sequences are eliminated. The coding mRNA sequence can be described as a unit of three nucleotides called a codon. 2. In Table 1., transcribe your DNA strand into the “language” of mR ...
Genetic Engineering
Genetic Engineering

... A small, circular DNA molecule that carries genes separate from the main DNA of a bacterial cell ...
Big Idea 3 Test Review - Class Pages
Big Idea 3 Test Review - Class Pages

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ACADEMIC BIOLOGY MIDTERM REVIEW GUIDE
ACADEMIC BIOLOGY MIDTERM REVIEW GUIDE

... 10. What are sex-linked traits? 11. Who shows more sex linked traits? 12. What is a carrier? Can a male be a carrier? 13. Be able to solve sex-linked punnett squares and give genotypic and phenotypic ratio of offspring. 14. Name two sex linked traits 15. What can be learned by looking at a karyotype ...
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Chapter 12 - Cloudfront.net
Chapter 12 - Cloudfront.net

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Practical Application of DNA Technology

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Molecular Biology Fourth Edition
Molecular Biology Fourth Edition

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Genetic Engineering

***********X***********X*******X*******X***X***X***X***X***X***X
***********X***********X*******X*******X***X***X***X***X***X***X

... Most genes are uninterrupted in yeast, but most genes are interrupted in flies and mammals. ...
BCH 550 Chromosome - Home - KSU Faculty Member websites
BCH 550 Chromosome - Home - KSU Faculty Member websites

... chromosomes into short (p i.e. petit) and long (q e.g. g=grand) arms. The tip of each chromosome is called telomere. • The exact function of the centromere is not clear, but it is known to be responsible for the movement of the chromosomes at cell division. ...
7.03 Fall 2003 Problem Set #3 Solutions
7.03 Fall 2003 Problem Set #3 Solutions

... (a) We are analyzing mutagens that specifically induce G·C A·T mutations in DNA. Therefore, we must determine the potential double stranded DNA sequences that will encode stop codons after going through this specific mutation. We will start with 5'UAG3'. The double stranded DNA that corresponds to 5 ...
Polymerase Chain Reaction
Polymerase Chain Reaction

Lab: DNA Extraction from Human Cheek Cells
Lab: DNA Extraction from Human Cheek Cells

... specially packaged through a series of events to fit easily in the cell’s nucleus. The structure of DNA, the double helix, is wrapped around proteins, folded back onto itself, and coiled into a compact chromosome. Individual chromosomes can be studied using microscopes, but the double helix of a chr ...
Genetics 2
Genetics 2

... It is by using this technology that scientists can determine if one person is related to another person, or if cells left at the scene of a crime may or may not correspond with the DNA of a suspect. The DNA from a person=s cells can be isolated and subjected to a restriction enzyme that is associat ...
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Replisome



The replisome is a complex molecular machine that carries out replication of DNA. The replisome first unwinds double stranded DNA into two single strands. For each of the resulting single strands, a new complementary sequence of DNA is synthesized. The net result is formation of two new double stranded DNA sequences that are exact copies of the original double stranded DNA sequence.In terms of structure, the replisome is composed of two replicative polymerase complexes, one of which synthesizes the leading strand, while the other synthesizes the lagging strand. The replisome is composed of a number of proteins including helicase, RFC, PCNA, gyrase/topoisomerase, SSB/RPA, primase, DNA polymerase I, RNAse H, and ligase.
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