Microbial Models: Genetics of Viruses and Bacteria
... plasmids which is also a ring • mutations are rare events but they impact genetic diversity in bacteria because of their rapid reproductive rate • Mutation is a major source of genetic variation in bacteria but not in more slowly reproducing organisms which have sexual reproduction ...
... plasmids which is also a ring • mutations are rare events but they impact genetic diversity in bacteria because of their rapid reproductive rate • Mutation is a major source of genetic variation in bacteria but not in more slowly reproducing organisms which have sexual reproduction ...
Ch. 9: Presentation Slides
... complementary DNA (cDNA). Like other DNA polymerases, reverse transcriptase requires a primer • The stretch of A nucleotides found at the 3' end of eukaryotic mRNA serves as a priming site. The primer can be a poly-T oligonucleotide • The resulting full-length cDNA contains an uninterrupted by intro ...
... complementary DNA (cDNA). Like other DNA polymerases, reverse transcriptase requires a primer • The stretch of A nucleotides found at the 3' end of eukaryotic mRNA serves as a priming site. The primer can be a poly-T oligonucleotide • The resulting full-length cDNA contains an uninterrupted by intro ...
Mutations and Gene Regulation
... • Remember: In Eukaryotes, DNA is found in the nucleus. • For the most part, all of the cells in an organism’s body have the same DNA. However, we have learned in the past that cells are specialized for specific tasks and parts of the body. • How can they be specialized but have the exact same DNA, ...
... • Remember: In Eukaryotes, DNA is found in the nucleus. • For the most part, all of the cells in an organism’s body have the same DNA. However, we have learned in the past that cells are specialized for specific tasks and parts of the body. • How can they be specialized but have the exact same DNA, ...
Plasmids - winterk
... Plasmids have been key to the development of molecular biotechnology. They act as delivery vehicles, or vectors, to introduce foreign DNA into bacteria. Using plasmids for DNA delivery began in the 1970s when DNA from other organisms was first ‘cut and pasted’ into specific sites within the plasmid ...
... Plasmids have been key to the development of molecular biotechnology. They act as delivery vehicles, or vectors, to introduce foreign DNA into bacteria. Using plasmids for DNA delivery began in the 1970s when DNA from other organisms was first ‘cut and pasted’ into specific sites within the plasmid ...
Competence
... requires breakage of double-stranded DNA and degradation of one of the two strands so that a linear single strand can enter the cells. 2. They must recyclize. However, pieces of plasmid or phage DNA can not recyclize if there are no repeated or ...
... requires breakage of double-stranded DNA and degradation of one of the two strands so that a linear single strand can enter the cells. 2. They must recyclize. However, pieces of plasmid or phage DNA can not recyclize if there are no repeated or ...
Oscar D. Kirstein*, Ibrahim Abassi, Araya Gebre Selassie •, Asrat
... Although only female sand flies transmit Leishmania parasites when they suck blood required for maturation of the egg, both sexes feed on plant-derived sugars by imbibing plant sap, nectar and/or honeydew. Importantly, components of the plant meals can influence the vector potential of sand fly fema ...
... Although only female sand flies transmit Leishmania parasites when they suck blood required for maturation of the egg, both sexes feed on plant-derived sugars by imbibing plant sap, nectar and/or honeydew. Importantly, components of the plant meals can influence the vector potential of sand fly fema ...
Honor Genetics DNA structure and replication
... – Begins at special sites called origins of replication, where the two strands are separated ...
... – Begins at special sites called origins of replication, where the two strands are separated ...
4.4 PCR, Electrophoresis, DNA profiling
... 4.4.2 State that, in gel electrophoresis, fragments of DNA move in an electric field and are separated according to their size. ...
... 4.4.2 State that, in gel electrophoresis, fragments of DNA move in an electric field and are separated according to their size. ...
Lesson Overview
... The Work of Watson and Crick At the same time, James Watson, an American biologist, and Francis Crick, a British physicist, were also trying to understand the structure of DNA. They built three-dimensional models of the molecule. ...
... The Work of Watson and Crick At the same time, James Watson, an American biologist, and Francis Crick, a British physicist, were also trying to understand the structure of DNA. They built three-dimensional models of the molecule. ...
10 Useful RNA Facts
... short chain of nucleotides. RNA can be shaped like a single helix, a straight molecule, or may be bet or twisted upon itself. DNA, in comparison, is double-stranded and consists of a very long chain of nucleotides. ...
... short chain of nucleotides. RNA can be shaped like a single helix, a straight molecule, or may be bet or twisted upon itself. DNA, in comparison, is double-stranded and consists of a very long chain of nucleotides. ...
3` Untranslated Regions
... Roles of Non-coding DNA Expressed as RNA Introns - May contain genes expressed independently of the exons they fall between. Many introns code for small nuclear RNAs (snoRNAs). These accumulate in the nucleolus, and may play a role in ribosome assembly. Thus the introns cut out of pre-mRNA may play ...
... Roles of Non-coding DNA Expressed as RNA Introns - May contain genes expressed independently of the exons they fall between. Many introns code for small nuclear RNAs (snoRNAs). These accumulate in the nucleolus, and may play a role in ribosome assembly. Thus the introns cut out of pre-mRNA may play ...
Genetic engineering
... 2) A primer is added to get DNA synthesis started 3) Nucleotides are added along with DNA polymerase so that each template strand is used to build a complementary strad. But some of the nucleotides are modified so that they terminate DNA synthesis – these bases are tagged so that they can be easily ...
... 2) A primer is added to get DNA synthesis started 3) Nucleotides are added along with DNA polymerase so that each template strand is used to build a complementary strad. But some of the nucleotides are modified so that they terminate DNA synthesis – these bases are tagged so that they can be easily ...
zChap11_140901 - Online Open Genetics
... 11.1 -omics techniques allow you to examine thousands of genes or protein at the same time. On a per-gene basis, this is more cost effective than examining a single gene at a time. However, the total cost of –omics technologies can be quite high (especially the instrumentation). Besides being more e ...
... 11.1 -omics techniques allow you to examine thousands of genes or protein at the same time. On a per-gene basis, this is more cost effective than examining a single gene at a time. However, the total cost of –omics technologies can be quite high (especially the instrumentation). Besides being more e ...
2013 Training Handout
... DNA polymerase (III) proceeds along a single-stranded molecule of DNA, recruiting free dNTP's (deoxy-nucleotide-triphosphates) to hydrogen bond with their appropriate complementary dNTP on the single strand (A with T and G with C), and to form a covalent phosphodiester bond with the previous nucleot ...
... DNA polymerase (III) proceeds along a single-stranded molecule of DNA, recruiting free dNTP's (deoxy-nucleotide-triphosphates) to hydrogen bond with their appropriate complementary dNTP on the single strand (A with T and G with C), and to form a covalent phosphodiester bond with the previous nucleot ...
DNA Profiling - Miss Jan`s Science Wikispace
... same restriction enzyme must be used to cut both the gene and the plasmid as this will produce the same sticky ends that can then be joined together by DNA ligase.. HOW – Use of specific restriction enzymes has enabled the cutting of DNA at a recognition site specific to that enzyme. For example the ...
... same restriction enzyme must be used to cut both the gene and the plasmid as this will produce the same sticky ends that can then be joined together by DNA ligase.. HOW – Use of specific restriction enzymes has enabled the cutting of DNA at a recognition site specific to that enzyme. For example the ...
6 Possible Alleles
... The TH01 locus contains repeats of TCAT. CCC TCAT TCAT TCAT TCAT TCAT TCAT AAA This example has 6 TCAT repeats. ...
... The TH01 locus contains repeats of TCAT. CCC TCAT TCAT TCAT TCAT TCAT TCAT AAA This example has 6 TCAT repeats. ...
mutations
... separate from its homologue during meiosis; resulting in one gamete receiving an extra copy of the chromosome (3 total) & another ...
... separate from its homologue during meiosis; resulting in one gamete receiving an extra copy of the chromosome (3 total) & another ...
Overview: The Flow of Genetic Information • The information content
... Overview: The Flow of Genetic Information • The information content of DNA is in the form of specific sequences of nucleotides • The DNA inherited by an organism leads to specific traits by dictating the synthesis of proteins • Proteins are the links between genotype and phenotype • Gene expression, ...
... Overview: The Flow of Genetic Information • The information content of DNA is in the form of specific sequences of nucleotides • The DNA inherited by an organism leads to specific traits by dictating the synthesis of proteins • Proteins are the links between genotype and phenotype • Gene expression, ...
Cauliflower mosaic virus: still in the news
... transcription start site (Sanfaçon and Hohn, 1990). The polyadenylation signal consists of an AAUAAA sequence which determines the cleavage of the CaMV transcripts 13 nt downstream and cis-acting upstream elements that increase the efficiency of the 3′ processing (Sanfaçon et al., 1991). The 35S RNA ...
... transcription start site (Sanfaçon and Hohn, 1990). The polyadenylation signal consists of an AAUAAA sequence which determines the cleavage of the CaMV transcripts 13 nt downstream and cis-acting upstream elements that increase the efficiency of the 3′ processing (Sanfaçon et al., 1991). The 35S RNA ...
CHAPTER 14 LECTURE NOTES: RECOMBINANT DNA
... B. Restriction enzyme mapping – Frequently it is important to have a restriction enzyme site map of a cloned gene for further manipulations of the gene. This is accomplished by digestion of the gene singly with several enzymes and then in combinations. The fragments are subjected to gel electrophore ...
... B. Restriction enzyme mapping – Frequently it is important to have a restriction enzyme site map of a cloned gene for further manipulations of the gene. This is accomplished by digestion of the gene singly with several enzymes and then in combinations. The fragments are subjected to gel electrophore ...
Replisome
The replisome is a complex molecular machine that carries out replication of DNA. The replisome first unwinds double stranded DNA into two single strands. For each of the resulting single strands, a new complementary sequence of DNA is synthesized. The net result is formation of two new double stranded DNA sequences that are exact copies of the original double stranded DNA sequence.In terms of structure, the replisome is composed of two replicative polymerase complexes, one of which synthesizes the leading strand, while the other synthesizes the lagging strand. The replisome is composed of a number of proteins including helicase, RFC, PCNA, gyrase/topoisomerase, SSB/RPA, primase, DNA polymerase I, RNAse H, and ligase.