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Go-ChIP-Grade™ Purified anti-Histone H3 (C-terminus

Class 10 Heredity and Evolution CBSE Solved Test paper-2
Class 10 Heredity and Evolution CBSE Solved Test paper-2

... chromosomes. If a sperm carrying x-chromosome fertilizes with the ovum, then sex of the baby will be female. If a sperm carrying y-chromosome fuses with the ovum, the sex of the baby will be male. 3. By comparing the similarity of nucleotide sequences in DNA of different kinds of organisms, evoluti ...
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Chromatin structure - U of L Class Index

Topic Definition 3` Refers to the third carbon of the nucleic acid
Topic Definition 3` Refers to the third carbon of the nucleic acid

... A procedure to separate molecules in an electric field. DNA migration takes place through a gel matrix (agarose or polyacrylamide) that acts as a molecular sieve. Since DNA is negatively charged, when placed in an electric field, it is attracted toward the positive electrode. Because the negative ch ...
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Transcription and Translation

... for ONE amino acid  Each three-letter unit on mRNA is called a codon  Most amino acids have more than one codon!  There are 20 amino acids with a possible 64 different triplets  The code is nearly universal among living organisms ...
GENETICS AND PARENTAGE TESTING CELL The unit from which
GENETICS AND PARENTAGE TESTING CELL The unit from which

... contains many genes, and each gene is located at a particular site, or locus, on the chromosome. The chromosome contains a very long single strand of the nucleic acid DNA, which is divided into small units called genes. The genes determine the hereditary characteristics, such as colour and size, of ...
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... The knowledge of gene activity arose from the experiments of several investigators. Garrod reasoned the cause for inborn errors of metabolism. Beadle and Tatum, working with red bread mold, suggested the one gene— one enzyme hypothesis. Pauling and Itano refined this to the one gene—one polypeptide ...
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... The zinc finger is a common structural motif in many protein DNA binding domains. It is found in roughly 3%(!) of all proteins and performs a diverse range of functions not limited to enhancing or inhibiting one specific gene. They are one of several domains that evolved to solve the problem of sequ ...
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Introduction of an Active DNA Microarray Fabrication for Medical

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... convective mixing caused by small differences in temperature or solute concentration, the tube contains a continuous shallow gradient of sucrose that increases in concentration toward the bottom of the tube (typically from 5% to 20% sucrose). Following centrifugation, the different components can be ...
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Automation of Genomic DNA Isolation with Nucleic

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The crystal structure of the complex between a disaccharide
The crystal structure of the complex between a disaccharide

... However, with respect to the position observed in doxorubicin complexes, a rotation of ~45°around the O7-C1¢ bond brings it away from the base pairs. The sugar is in chair conformation as in doxorubicin complexes, with the hydroxyl group in the 3¢ position still in van der Waals contact with the gua ...
Protein Synthesis - Pascack Valley Regional High School
Protein Synthesis - Pascack Valley Regional High School

... • Messenger RNA (mRNA) copies DNA’s code & carries the genetic information to the ribosomes • Ribosomal RNA (rRNA), along with protein, makes up the ribosomes • Transfer RNA (tRNA) transfers amino acids to the ribosomes where proteins are synthesized copyright cmassengale ...
Recombinant DNA
Recombinant DNA

... DNA that gets into the bacterial cell) 2. Restriction enzymes or endonucleases from bacterial cells are key a. Recognize and cleave only very specific sequence of DNA b. Some make a staggered cut of the DNA yielding sticky ends (single stranded nucleotides bases capable of binding with complimentary ...
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... strands to separate (unzip).  Cool the mixture and add a primer, a short sequence of base pairs that will add to its complementary sequence on the DNA strand.  Finally, add a DNA polymerase and a mixture of free nucleotides to the separated strands. Heat again to around 75° C for the completion. C ...
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... 2. The two subunits of the ribosome come together 3. The first amino acid with its attached tRNA ...
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Microbial Genetics Lecture PowerPoint

... Understanding how beta-lactamase gene is turned on/off, can help us to design a drug to disable that gene’s expression (turn off the gene), making penicillin-resistant strains of Staph again vulnerable to penicillin. ...
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... had been designed as part of an eort to promote contact between dierent MRC research groups. By 1962, when Watson, Crick, and Wilkins won the Nobel Prize for physiology/medicine, Franklin had died. The Nobel Prize only goes to living recipients, and can only be shared among three winners. Were she ...
recombinant dna
recombinant dna

... terminate in mortise-like sticky ends.  Each sticky end is composed ...
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Replisome



The replisome is a complex molecular machine that carries out replication of DNA. The replisome first unwinds double stranded DNA into two single strands. For each of the resulting single strands, a new complementary sequence of DNA is synthesized. The net result is formation of two new double stranded DNA sequences that are exact copies of the original double stranded DNA sequence.In terms of structure, the replisome is composed of two replicative polymerase complexes, one of which synthesizes the leading strand, while the other synthesizes the lagging strand. The replisome is composed of a number of proteins including helicase, RFC, PCNA, gyrase/topoisomerase, SSB/RPA, primase, DNA polymerase I, RNAse H, and ligase.
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